Dexamethasone blocks astroglial differentiation from neural precursor cells.

Abstract:

:In previous studies, we demonstrated functional neuronal and dopaminergic differentiation of fetal mesencephalic neural precursor cells. The major factors for orienting their progeny towards a dopaminergic phenotype are forskolin and interleukin-1beta. Here, we investigated the effects of dexamethasone (10 microM) on neuronal and glial differentiation. Exposure of mesencephalic neural precursor cells to dexamethasone significantly reduces the amount of glial fibrillary acidic protein astroglia, but not of galactocerebrosidase C oligodendroglia, MAP2ab neurons and tyrosine hydroxylase dopaminergic cells. Presuming a possible involvement of the nuclear factor-kappaB pathway, we examined the effects of wortmannin (phosphatidylinositol 3'-kinase inhibitor) and SN50 (nuclear factor-kappaB inhibitor) on gliogenesis. Both wortmannin and SN50 mimicked the effects of dexamethasone suggesting that dexamethasone specifically blocks astroglial differentiation from mesencephalic neural precursor cells most likely via inhibition of the nuclear factor-kappaB pathway.

journal_name

Neuroreport

journal_title

Neuroreport

authors

Sabolek M,Herborg A,Schwarz J,Storch A

doi

10.1097/01.wnr.0000236862.08834.50

subject

Has Abstract

pub_date

2006-11-06 00:00:00

pages

1719-23

issue

16

eissn

0959-4965

issn

1473-558X

pii

00001756-200611060-00012

journal_volume

17

pub_type

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