Abstract:
:We reported previously that Lixelle, which was used for beta-2 microglobulin (BMG) adsorption columns, could adsorb not only BMG but also inflammatory cytokines. We then were interested in the application of Lixelle to patients with systemic inflammatory response syndrome (SIRS) and tried to find out its ability to adsorb microorganism components in vitro using lipopolysaccharide (LPS) (E. coli: B8), endotoxin (ET) containing water, and peptidoglycan (PG: Micrococcus luteus). The initial concentrations of each solution were LPS (ET: 29,135 EU/L), contaminated water (ET: 3,523 EU/L), and PG (67.1 ng/ml) and 2.5 ml of each of the stock solutions and adjusted diluted solutions contained 0.5 ml of Lixelle beads. After shaking at 37 degrees C for 2 h, ET in the solutions was determined by the ET specific-limulus amebocyte lysate (ES-LAL) method and PG by the silkworm larbae plasma (SLP) method. The results revealed that even when ET concentrations in LPS and contaminated water were high, the samples containing Lixelle beads showed significant decreases. There was some adsorption of PG but no significant differences. Thus, Lixelle beads can adsorb not only BMG but also microorganism components such as ET and PG. These findings, together with the ability to adsorb inflammatory cytokines by Lixelle, show the possibility of application for the treatment of infectious SIRS.
journal_name
Artif Organsjournal_title
Artificial organsauthors
Tsuchida K,Takemoto Y,Nakatani T,Nakamura T,Sugimura K,Yamamoto Kdoi
10.1046/j.1525-1594.2000.06502-2.xsubject
Has Abstractpub_date
2000-07-01 00:00:00pages
577-80issue
7eissn
0160-564Xissn
1525-1594pii
aor6502-2journal_volume
24pub_type
杂志文章abstract::Oxidative stress is crucial in red blood cell (RBC) damage induced by activated neutrophils in in vitro experiments. The aim of the study was to evaluate whether the bioincompatibility phenomena occurring during hemodialysis (HD) (where neutrophil activation with increased free radical production is well documented) m...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1046/j.1525-1594.2000.06432.x
更新日期:2000-01-01 00:00:00
abstract::Short-term diabetes was induced in male Wistar rats with streptozotocin injection. The effects of diabetes on latissimus dorsi (LD) muscle contractile and biochemical properties and acute cardiomyoplasty (CDM) were assessed and compared with data from 16 control rats. Isometric force, contractile properties, and fatig...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/j.1525-1594.2004.47349.x
更新日期:2004-04-01 00:00:00
abstract::The effectiveness of the blood substitute neo red cells (NRC) on hemorrhagic shock was evaluated in dogs. Shock was induced by bloodletting, and NRC was infused immediately until the dogs recovered from shock; the process was repeated 3 times (mild shock), or NRC was infused after shock was maintained for 30 min, and ...
journal_title:Artificial organs
pub_type: 杂志文章
doi:
更新日期:1998-02-01 00:00:00
abstract::In clinical settings, no method has been established to examine the fatigue of a latissimus dorsi muscle (LDM) preconditioned for cardiomyoplasty. We examined the feasibility of measuring muscle stiffness (tactile stiffness) to evaluate muscle fatigue in situ using our tactile sensor. We stimulated canine LDM with bur...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1046/j.1525-1594.2000.06551.x
更新日期:2000-10-01 00:00:00
abstract::Central extracorporeal life support (ECLS) is an effective treatment method of cardiogenic shock patients with or without lung failure. However, complications like infection and bleeding are common. The classical implantation approach requires full sternotomy, mobilization of the heart, with the well-known risks of bl...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/aor.12344
更新日期:2015-02-01 00:00:00
abstract::The flow of blood as a viscous, Newtonian, incompressible liquid in an expanding spherical cavity with a ball-type inlet valve was examined. This type of flow can serve as a model of the phase of ventricular diastole in a sac-type artificial heart. The critical values of Reynolds numbers at which flow interruption beh...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/j.1525-1594.1983.tb04170.x
更新日期:1983-02-01 00:00:00
abstract::A multi-compartment capillary membrane culture model with independently perfused three-dimensionally woven capillaries was developed for immobilization of hepatocytes in bioreactors. This enables spatial restructuring of cells and enhanced mass transfer performance with more efficient oxygenation and metabolite exchan...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/j.1525-1594.1994.tb02181.x
更新日期:1994-03-01 00:00:00
abstract::The aim of this study was to verify if dialysis solution volumes used in patients on continuous ambulatory peritoneal dialysis (CAPD) interfere with pulmonary function and if the pulmonary volumes interfere with the lymphatic absorption of the peritoneal cavity. We submitted 10 CAPD patients with a mean age of 48 +/- ...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1046/j.1525-1594.2001.06904.x
更新日期:2001-11-01 00:00:00
abstract::Microcapsules containing insulin crystals or islets of Langerhans were made by extruding a mixture of insulin crystals or islets and sodium alginate into a calcium chloride solution, and then coating it with poly-l-lysine. When these microcapsules were incubated at 37 degrees C, insulin could be detected readily in th...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/j.1525-1594.1983.tb04187.x
更新日期:1983-05-01 00:00:00
abstract::For introduction of a left atrial (LA) cannula by the transseptal puncture technique, we examined the feasibility of using biplane transesophageal echocardiography (B-TEE). A transseptal puncture was performed on 15 patients (3 male; 12 female; mean age, 48.9 +/- 11.2 years) by B-TEE guide during percutaneous transven...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/j.1525-1594.1992.tb00537.x
更新日期:1992-08-01 00:00:00
abstract::Atrial connections in a single-unit total artificial heart (TAH) may be difficult to make because of the rigidity of the device and the fixed position of the atrial inlets. We developed a technique to separate the natural atrial borders in an experimental implantation of a unitary TAH. In this technique, the interatri...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/j.1525-1594.1995.tb02442.x
更新日期:1995-08-01 00:00:00
abstract::Thousands of ventricular assist devices (VADs) currently provide circulatory support to patients worldwide, and dozens of heart pump designs for adults and pediatric patients are under various stages of development in preparation for translation to clinical use. The successful bench-to-bedside development of a VAD inv...
journal_title:Artificial organs
pub_type: 杂志文章,评审
doi:10.1111/aor.12600
更新日期:2016-06-01 00:00:00
abstract::A comparative study of the thrombogenicity of several microporous membranes was performed using an ex vivo system simulating the conditions in an artificial lung. A study of platelet adhesion and adsorbed protein layer indicated that the membranes separated into three groups. Copolyurethane membranes adsorbed very few...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/j.1525-1594.1985.tb04343.x
更新日期:1985-02-01 00:00:00
abstract::Cardiopulmonary bypass (CPB), an integral part of valve surgery, is known to cause hemodilution, which can lead to a need for blood transfusion as well as a systemic inflammatory reaction. Our aim was to evaluate a reduced priming (RP) system for CPB with regard to clinical outcomes in patients undergoing valve surger...
journal_title:Artificial organs
pub_type: 杂志文章,随机对照试验
doi:10.1111/j.1525-1594.2006.00275.x
更新日期:2006-08-01 00:00:00
abstract::The use of recombinant human erythropoietin (rHuEpo) has revolutionized anemia management of early and late stages of chronic kidney disease. Darbopoietin is also now available for the treatment of anemia of chronic kidney disease. In addition, rHuEpo has been used for the treatment of anemia observed in critical illn...
journal_title:Artificial organs
pub_type: 杂志文章,评审
doi:10.1046/j.1525-1594.2003.07287.x
更新日期:2003-09-01 00:00:00
abstract::Aortic wall thinness was one of the most characteristic changes observed in experimental animals under prolonged continuous flow left heart bypass. The goal of this study was to determine the roles of smooth muscle cells in the vascular remodeling process in cases demonstrating aortic wall thinness under prolonged con...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1046/j.1525-1594.2003.00022.x
更新日期:2003-10-01 00:00:00
abstract::We compared in vitro rates of hemolysis for a recently developed centrifugal pump with a conventional roller pump (10-10-00; Stöckert, Munich, Germany). Flow rates of 0.3 L/min and 1 L/min and a pressure of 200 mm Hg were chosen to simulate conditions during neonatal extracorporeal membrane oxygenation (ECMO). There w...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/j.1525-1594.1996.tb04485.x
更新日期:1996-05-01 00:00:00
abstract::Many patients all over the world suffer from acute wounds caused by traumas or burns. In most crucial cases, skin regeneration cannot be promoted spontaneously, and skin grafts are applied as the main treatment. However, this therapy has some drawbacks which motivate researchers to develop wound dressings. In this stu...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/aor.12682
更新日期:2016-08-01 00:00:00
abstract::Existing peritoneal dialysis (PD) solutions were formulated for the maintenance of fluid and electrolyte balance, correction of metabolic acidosis, and the removal of metabolic waste products. Solutions currently in development are designed to improve biocompatibility by neutralizing pH and reducing glucose degradatio...
journal_title:Artificial organs
pub_type: 杂志文章,评审
doi:10.1046/j.1525-1594.1998.06022.x
更新日期:1998-01-01 00:00:00
abstract::For some time now researchers have argued the efficacy of pulsatile versus nonpulsatile hemodynamic support. Pulsatile systems, while providing a more physiological pumping source, are burdened with a multiparametric output function that can greatly affect the utility of such ventricular assist device (VAD) support. A...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/j.1525-1594.1992.tb00299.x
更新日期:1992-04-01 00:00:00
abstract::Giant cerebral aneurysms may be untreatable by conventional neurosurgical techniques. Early attempts to use circulatory assistance and deep hypothermia were abandoned due to hemorrhagic complications. More recently, interest in circulatory support for complex neurosurgical procedures has been renewed. A consecutive se...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1046/j.1525-1594.2000.06594.x
更新日期:2000-06-01 00:00:00
abstract::Pyridoxalated-hemoglobin-polyoxyethylene conjugate (PHP), which is made from out-dated human red blood cells by two major chemical modifications, namely pyridoxalation and conjugation with polyoxyethylene (POE), is currently under development as a physiological oxygen carrier. This study assessed the effects of PHP-88...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/j.1525-1594.1993.tb00424.x
更新日期:1993-03-01 00:00:00
abstract::Although the debate still continues over the effectiveness of pulsatile versus nonpulsatile perfusion, it has been clearly proven that there are several significant physiological benefits of pulsatile perfusion during cardiopulmonary bypass (CPB) compared to nonpulsatile perfusion. However, the components of the extra...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1046/j.1525-1594.1998.06017.x
更新日期:1998-08-01 00:00:00
abstract::The implementation of simultaneous mechanical cavopulmonary assistance having blood pumps located in both of the vena cavae is investigated as an approach to treating patients with an ailing Fontan physiology. Identical intravascular blood pumps are employed to model the hemodynamic support of a patient-specific Fonta...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/aor.12039
更新日期:2013-06-01 00:00:00
abstract::A review and analysis of 5,001 neonatal venoarterial (VA) extracorporeal membrane oxygenation (ECMO) cases showed that bacterial and fungal infection occurred in 147 (2.9%) and 26 (0.6%) patients, respectively, with an overall incidence of 3.5%. Bivariate analysis was used to compare infected infants with controls, ba...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/j.1525-1594.1996.tb04428.x
更新日期:1996-03-01 00:00:00
abstract::The heparin-conjugated fibrin (HCF) system has been developed to deliver bone morphogenetic proteins (BMPs) for a long-term period and thus enhance bone regeneration. In the present study, we tested the effectiveness of the delivery system for spinal fusion with a very low dose of BMP-2. A total of 15 rabbits underwen...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/j.1525-1594.2012.01578.x
更新日期:2013-05-01 00:00:00
abstract::We have developed a novel method to simultaneously evaluate thromboresistance of two different types of drip chambers with a filter used in extracorporeal blood circuits for hemodialysis using canine arteriovenous shunt models. Bilateral bypasses between the femoral artery and vein on both sides of the animal were for...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/j.1525-1594.1997.tb00354.x
更新日期:1997-02-01 00:00:00
abstract::There is increasing evidence showing that adult stem cells are useful for tissue regeneration. Bone marrow mesenchymal stem cells (MSCs) are self-renewing and are potent in differentiating into multiple cells and tissues. To investigate the practicability of repairing burn wounds with tissue-engineered (TE) skin combi...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/j.1525-1594.2008.00654.x
更新日期:2008-12-01 00:00:00
abstract::A surgical method for the implantation of a mechanical right ventricular assist device which avoids resternotomy/thoracotomy and related complications at the time of explantation is presented. In order to support the failing right heart, a Dacron vascular graft was sewn to the pulmonary artery. For venous drainage, a ...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/j.1525-1594.2012.01520.x
更新日期:2012-08-01 00:00:00
abstract::Liposome-encapsulated hemoglobin (LEH) was proven to be protective in cerebral ischemia/reperfusion injury. The present study evaluated LEH in a rat model of permanent middle cerebral artery (MCA) occlusion to clarify its effect during ischemia and reperfusion. Five minutes after thread occlusion of the MCA, rats were...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/j.1525-1594.2008.00700.x
更新日期:2009-02-01 00:00:00