Abstract：
:Eight isolates of potato virus Y NTN strain (PVY-NTN) of different origin were studied by means of monoclonal antibodies (MAbs) in non-competitive and competitive enzyme-linked immunosorbent assay (ELISA), and by immunoblot analysis of the viral coat protein (CP). As the MAbs reacted with the denatured viral CP, their epitopes must be continuous. The ELISA data demonstrate that the epitopes are topologically different. The epitopes may be located on the N-terminal part of CP as showed its partial amino acid sequencing and the immunoblot analysis.
journal_name
Acta Viroljournal_title
Acta virologicaauthors
Cerovská N,Moravec T,Filigarová M,Ryslavá H,Grosclaude Jsubject
Has Abstractpub_date
1999-12-01 00:00:00pages
391-3issue
6eissn
0001-723Xissn
1336-2305journal_volume
43pub_type
杂志文章相关文献
ACTA VIROLOGICA文献大全abstract：:Reproduction of Hazara virus in intracerebrally (ic) inoculated newborn white mice was studied by virological, serological and histological methods, electron microscopy and the direct fluorescent antibody technique. In the course of infection the virus was detectable in the blood, brain and some viscera, the largest a...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1977-03-01 00:00:00
abstract：:The present paper describes first full genome sequence of the Garlic virus D (GarV-D) from northern India with a genome size of 8425 bp long ssRNA. The infected leaves and bulbs of garlic variety Yamuna Safed (G-282) plants suspected for GarV-D infection were collected with the aim to identify contagion virus during M...
journal_title：Acta virologica
pub_type： 杂志文章
doi：10.4149/av_2020_405
更新日期：2020-01-01 00:00:00
abstract：:We characterized leukocytes in peripheral blood of BALB/c mice infected with mouse herpesvirus isolate 72 (MHV-72) representing an isolate of mouse herpesvirus strain 68 (MHV-68, species Murid herpesvirus 4, genus Rhadinovirus, subfamily Gammaherpesvirinae, family Herpesviridae) (van Regenmortel et al., 2000). In acut...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：2002-01-01 00:00:00
abstract：:This study reports on our experience with whole herpes simplex vaccines in Bulgaria for a period of 17 years. More than 1,500 immunized patients with herpes ophtalmicus showed a reduction of the recovery period, reduced number of the recurrences and reduced risk of visual damage. More than 14,000 patients suffering fr...
journal_title：Acta virologica
pub_type： 临床试验,杂志文章
doi：
更新日期：1994-08-01 00:00:00
abstract：:In 1986 five avian paramyxovirus (PMV) strains were isolated in embryonated chicken eggs from sick children with influenza. The strains were identified as PMV-2 serotype due to the close antigenic relationships between their HN-proteins and of the reference PMV-2 strains isolated from different birds all over the worl...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1990-04-01 00:00:00
abstract：:To investigate whether Epstein-Barr virus (EBV) is associated with ordinary gastric carcinoma and to determine its genotype, samples from ordinary gastric carcinoma from 65 patients (40 males, 20 females) and 21 endoscopic biopsies from 7 individuals with non-neoplastic mucosa were analysed using one-stage and nested ...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1998-12-01 00:00:00
abstract：:The chemical nature of BK virus receptors on human erythrocytes was investigated. Glycosidases, KIO4, proteases, phospholipases and other substances acting specifically on different structures of plasma membranes were used to ascertain the role of surface cell components in the interaction with BK virus. The results o...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1981-07-01 00:00:00
abstract：:Human influenza virus A/Krasnodar/101/59 (H2N2) was passaged in chick fibroblast cultures in the presence of trypsin at suboptimal temperature. The virus which underwent 16 passages at 28 degrees C possessed cold-adapted (ca) and temperature sensitive (ts) phenotypes and formed larger plaques at the optimal temperatur...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1984-09-01 00:00:00
abstract：:Tick-borne viruses are causative agents of several important human diseases. Tick-borne encephalitis virus (TBEV) is the most prominent representative considered medically to be the most important arbovirus (arthropod-borne virus) in Europe and northern Asia. Tick-borne virus transmission cycles are determined by the ...
journal_title：Acta virologica
pub_type： 杂志文章,评审
doi：10.4149/av_2013_02_123
更新日期：2013-01-01 00:00:00
abstract：:Molecular analysis of histological hallmarks (neurofibrillary tangles, neuritic plaques, neuropil threads and dystrophic neurites) of Alzheimer diseased brain tissues showed that these lesions contain paired helical filaments. Their major constituent is microtubule associated protein tau that is in an abnormally hyper...
journal_title：Acta virologica
pub_type： 杂志文章,评审
doi：
更新日期：1994-06-01 00:00:00
abstract：:In the sera of 17 patients with nasopharyngeal carcinoma (NPC) and of 19 patients with tonsillar carcinoma (TC) the titres of IgA, IgG and IgM antibodies to EBV VCA (viral capsid antigen) and of IgG antibodies to EBV EA (early antigen) were determined by the indirect immunofluorescence (IF) method. Significant differe...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1983-05-01 00:00:00
abstract：:The dsRNA profiles of the Czechoslovakian and Siberian serotypes of the Kemerovo serocomplex viruses examined were similar in agarose, while their dsRNA profiles were distinct in polyacrylamide gel. Blot hybridization studies of the Kemerovo serocomplex viruses demonstrated that the genes were highly conserved among t...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1988-09-01 00:00:00
abstract：:2-0-hexadecyl-glycero-3-phosphocholine (GPC) 1 mmol/l solution reduced the infectivity of red clover mottle virus (RCMV) by over 90%. The binding of GPC molecules to RCMV particles was investigated with 1H-nmr spectroscopy, which showed a striking interaction between virus particles and GPC molecules. In 10 mmol/l GPC...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1987-03-01 00:00:00
abstract：UNLABELLED:Influenza A (H1N1) virus is an important pathogen that can be transmitted in the enzootic cycle involved in influenza epidemics. In this study, we analyzed natural selection on the 260 sequences of subtype 1 hemagglutinin (HA1) gene of H1N1 viruses isolated from humans and swine in Asia. Nonsynonymous and sy...
journal_title：Acta virologica
pub_type： 杂志文章
doi：10.4149/av_2010_02_113
更新日期：2010-01-01 00:00:00
abstract：:Structural proteins V1, V2 and V3 of Japanese encephalitis virus (JEV) were isolated and purified by means of polyacrylamide gel electrophoresis (PAGE) in the presence of sodium dodecyl sulfate (SDS) and extracted from the sliced gel. The purified and renatured V3 envelope protein was found to bind to receptors for JE...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1982-09-01 00:00:00
abstract：:Phages are excellent models for studying the mechanism of DNA replication in prokaryotes. Identification of phage proteins involved in phage DNA replication is the first prerequisite for elucidation of the phage replication module. We focused on replication proteins gp41 (a putative helicase from SF2 superfamily), gp4...
journal_title：Acta virologica
pub_type： 杂志文章
doi：10.4149/av_2014_02_152
更新日期：2014-01-01 00:00:00
abstract：:In the summer 1999, a measles outbreak occurred in Uruguai. During this outbreak 58 cases were recorded, 36 of which were laboratory confirmed as positive for measles virus (MV) IgM. The cases occurred in touristic places (Montevideo and Maldonado) predominantly among health facilities and tourist service personnel. U...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：2000-02-01 00:00:00
abstract：:Transport properties of 9-(S)-(2,3-dihydroxypropyl) adenine (DHPA) in cell cultures were studied. Transport of DHPA into chick embryo (CE), ZP (a cell line derived from rabbit lungs) and HeLa cells reached equilibrium values after 10 min incubation. The concentration of intracellular DHPA varied from 30 to 50 per cent...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1983-03-01 00:00:00
abstract：:The coat protein (CP) gene of Prunus necrotic ringspot virus (PNRSV) was cloned into pET 16b vector and expressed in Escherichia coli. CP-enriched fractions were prepared from whole cell lysate by differential centrifugation. The fraction sedimenting at 20,000 x g for 30 mins was used for preparation of a rabbit antis...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：2001-02-01 00:00:00
abstract：:RNA interference (RNAi) is a biological phenomenon in which introduction of a small, double-stranded interfering RNAs (siRNAs) into a cell causes a specific degradation of homologous single-stranded RNA. siRNA can be delivered into the cell by different approaches including synthetic RNA, in vitro transcribed RNA and ...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：2005-01-01 00:00:00
abstract：:A herpes simplex virus type 1 (HSV-1)-specific transfer factor (TF), was separated and purified from the leukocyte dialysate of goats immunized with HSV-1 using affinity chromatography on antigen-sorbent and reversed phase high performance liquid chromatography (RP-HPLC). The antigen-specific activities of the startin...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1992-05-01 00:00:00
abstract：:To elucidate epidemiological interference between respiratory syncytial (RSV) and influenza viruses, the influence of influenza A (HlN1) virus on the growth of RSV was examined. Although RSV grew in MDCK cells, coinfection with influenza A virus led to a reduction of progeny RSV. The degree of growth interference depe...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：2000-04-01 00:00:00
abstract：:14C-labelled Lassa virus was purified by isopycnic centrifugation and split to subvirion fractions. The purified virus was treated with nonionic detergents Nonidet P-40 (NP-40) and with octylglycoside. After ultracentrifugation in urographin density gradient, two subvirion fractions with buoyant density of 1.24-1.26 a...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1989-03-01 00:00:00
abstract：:The activities, the temperature and pH optima of in vitro functioning and stability upon heating of virion transcriptase of 10 human influenza virus A strains differing in reactogenicity and isolated in different epidemiological situations, and of fowl plague virus (FVP) were compared. As compared with virion transcri...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1978-03-01 00:00:00
abstract：:The presence of interferon and type A immunoglobulins (IgA) was followed up in the nasopharyngeal washings collected from volunteers immunized intranasally with an inactivated influenza vaccine [strain A/Rom 1/73 (H3N2)]. Interferon was detected 24 hours after vaccine administration, its incidence being similar to tha...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1975-05-01 00:00:00
abstract：:Nine samples of diseased Malvastrum coromandelianum plants collected from the fields in Yunnan province of China were found to be infected with Malvastrum yellow vein Yunnan virus (MYVYNV), when tested by PCR using specific primers. The results of PCR and Southern blot analysis showed that only 4 samples out of 9 were...
journal_title：Acta virologica
pub_type： 杂志文章
doi：10.4149/av_2010_01_21
更新日期：2010-01-01 00:00:00
abstract：:Restriction endonuclease fragment length polymorphism (RFLP) analysis and polymerase chain reaction (PCR) were used to characterize the genotypic diversity of 7 strains of Rickettsia conorii from South Africa, Ethiopia, Morocco, India and Russia. The strains of R. conorii were divided into four genotypes by Rsa I or P...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1994-02-01 00:00:00
abstract：:Defective viral particles (DVP) were isolated from the medium of chicken myeloblasts transformed by avian myeloblastosis virus (AMV) in the absence of helper viruses (non-producer myeloblasts). A 60S genomic RNA complex of AMV was isolated from DVP and translated in vitro. The predominant translation product was the g...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1993-04-01 00:00:00
abstract：:A limited epidemic, with important mortality among the initial human cases, occured in a forest region in the north of Gabon by the end of 1994. It was identified as yellow fever according to first serological and reverse transcription/polymerase chain reaction (RT-PCR) results, but no virus was isolated. We received ...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1996-04-01 00:00:00
abstract：:Cell-mediated reactions in measles cases, in direct contacts and healthy children were tested. Indices of phytohaemagglutinin-stimulated blastic transformation and leukocyte migration inhibition were evaluated. Positive reactions were found in the first and second weeks after manifestation of clinical symptoms. The ap...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1980-03-01 00:00:00
