Abstract：
:The coat protein (CP) gene of Prunus necrotic ringspot virus (PNRSV) was cloned into pET 16b vector and expressed in Escherichia coli. CP-enriched fractions were prepared from whole cell lysate by differential centrifugation. The fraction sedimenting at 20,000 x g for 30 mins was used for preparation of a rabbit antiserum to CP. This antiserum had a titer of 1:2048 and reacted in a double-antibody sandwich ELISA (DAS-ELISA).
journal_name
Acta Viroljournal_title
Acta virologicaauthors
Petrzik K,Mráz I,Kubelková Dsubject
Has Abstractpub_date
2001-02-01 00:00:00pages
61-3issue
1eissn
0001-723Xissn
1336-2305journal_volume
45pub_type
杂志文章相关文献
ACTA VIROLOGICA文献大全abstract：:Five monoclonal antibodies (MoAbs) against Indian reference/vaccine strain of foot-and-mouth disease (FMD) virus subtype A22 (IND17/77) and a guinea pig antibody against a synthetic peptide representing amino acids (aa) 136-151 of VP1 polypeptide of A22 virus were used in the study. All the antibodies either failed to...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1997-06-01 00:00:00
abstract：:Some differences were established between mopyridone-sensitive (MCU-s) wild strain and mopyridone-resistant (MCU-r) mutant progenies of influenza virus A/Hong Kong/1/68 (H3N2). The virions of MCU-r mutant had a lower buoyant density in linear sucrose gradient as compared to those of MCU-s strain, and an increased abil...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1995-04-01 00:00:00
abstract：:Monoclonal antibodies (MAbs) were prepared against ICP4 of Marek's disease virus (MDV). Mice were inoculated with ICP4 obtained from High-Five insect cells infected with a recombinant baculovirus expressing ICP4. MAbs were selected by enzyme-linked immunosorbent assay (ELISA) using MDV-infected and control chick kidne...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1999-04-01 00:00:00
abstract：:Cytotoxic T lymphocyte (CTL) activity of draining lymph node (DLN) cells isolated from BALB/c mice infected with ectromelia virus (EV) was examined using a fluorometric cell-mediated cytotoxicity (CMC) assay. Specific lysis of target cells A20 and EMT-6 primed with EV was demonstrated. The classical CD8+ cytolytic pat...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1996-11-01 00:00:00
abstract：:The identification and genetic characterization of bovine viral diarrhea virus (BVDV) isolate 17237 detected in western Slovakia is described. The analysis of 5'-untranslated region (5'-UTR), autoprotease (Npro) gene, and structural genes (C, Erns, E1, E2) was carried out. The percentage of nucleotide and deduced amin...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：2008-01-01 00:00:00
abstract：:After passaging the NWS influenza virus at increasing temperatures in the hamster embryo fibroblast (HEF) cell line, a temperature-resistant (tr) mutant which grew well at 39 degrees C was isolated. Attempts were made to isolate temperature-sensitive (ts) mutants from the tr virus. After growth in the presence of 5-fl...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1976-08-01 00:00:00
abstract：:Haemocytes of laboratory bred half-engorged Hyalomma dromedarii ticks phagocytized intracoelomally inoculated Coxiella burnetii organisms. Significantly higher phagocytosis of phase II than phase I C. burnetii was observed, irrespective of whether live, killed untreated or killed organisms treated with chloroform-meth...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1984-01-01 00:00:00
abstract：:Structural proteins V1, V2 and V3 of Japanese encephalitis virus (JEV) were isolated and purified by means of polyacrylamide gel electrophoresis (PAGE) in the presence of sodium dodecyl sulfate (SDS) and extracted from the sliced gel. The purified and renatured V3 envelope protein was found to bind to receptors for JE...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1982-09-01 00:00:00
abstract：:In 1986 five avian paramyxovirus (PMV) strains were isolated in embryonated chicken eggs from sick children with influenza. The strains were identified as PMV-2 serotype due to the close antigenic relationships between their HN-proteins and of the reference PMV-2 strains isolated from different birds all over the worl...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1990-04-01 00:00:00
abstract：:A rickettsia-like organism (RLO) was detected in the oocytes of Haemaphysalis inermis ticks. The RLOs were about 5 microns long bent rods with a definite inner structure. They were Gram-negative and could be visualized by Giemsa but not by Gimenez staining. Attempts to cultivate the RLO in chick embryo yolk sacs, vari...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1981-09-01 00:00:00
abstract：:A limited epidemic, with important mortality among the initial human cases, occured in a forest region in the north of Gabon by the end of 1994. It was identified as yellow fever according to first serological and reverse transcription/polymerase chain reaction (RT-PCR) results, but no virus was isolated. We received ...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1996-04-01 00:00:00
abstract：:Current isolates of the subtypes H7N3 and H7N7 from 1979 to 1981 were examined and compared with the reference strains with regard to their antigenic variability and to their pathogenicity for birds and mammals in order to establish the potentiality of influenza A/H7 virus (Hav1) transmission from birds to mammals. Th...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1986-01-01 00:00:00
abstract：:The presence and the level of hepatitis B surface antigen (HBsAg)/IgM complexes were determined in 54 chronic HBsAg carriers in relation to receptors for polymerized human serum albumin (pHSA-R) tested by specific radioimmunoassay, and to hepatitis B virus-DNA polymerase (HBV-DNAp). HBsAg/IgM complexes, correlated sig...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1987-03-01 00:00:00
abstract：:Restriction endonuclease fragment length polymorphism (RFLP) analysis and polymerase chain reaction (PCR) were used to characterize the genotypic diversity of 7 strains of Rickettsia conorii from South Africa, Ethiopia, Morocco, India and Russia. The strains of R. conorii were divided into four genotypes by Rsa I or P...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1994-02-01 00:00:00
abstract：:Tick-borne viruses are causative agents of several important human diseases. Tick-borne encephalitis virus (TBEV) is the most prominent representative considered medically to be the most important arbovirus (arthropod-borne virus) in Europe and northern Asia. Tick-borne virus transmission cycles are determined by the ...
journal_title：Acta virologica
pub_type： 杂志文章,评审
doi：10.4149/av_2013_02_123
更新日期：2013-01-01 00:00:00
abstract：:Herpes simplex virus 1 (HSV-1) is one of the most prevalent human pathogens in both industrialized and developing countries. This study was performed to analyze the antiviral activity of purified flavonoid from Polygonum perfoliatum L. against HSV-1 infection in vitro and in vivo. Flavonoid showed no inhibitory effect...
journal_title：Acta virologica
pub_type： 杂志文章
doi：10.4149/av_2014_04_368
更新日期：2014-01-01 00:00:00
abstract：:In this study, DNA vaccines consisting of vector IRES-gD expressing Herpes simplex virus 1 (HSV-1) glycoprotein D (gD) and vector IRES-gD-IL-2 coexpressing HSV-1 gD and interleukin-2 (IL-2), respectively, were constructed. After intramuscular inoculation, both vaccines induced in BALB/c mice antibodies as assayed by E...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：2006-01-01 00:00:00
abstract：:Results of the direct immunofluorescence (IF) test in 152 clinical specimens (throat swabs) were compared with those of respiratory syncytial virus (RSV) isolation. The prevalence of RSV infections in the upper and lower respiratory tract was high especially in infants until 12 months of age. The average RSV isolation...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1987-09-01 00:00:00
abstract：:Eperythrozoon was detected in suckling mice inoculated with a suspension of nymphal Ixodes ricinus ticks. The organism was filterable through 220 nm Millipore membranes, moderately sensitive to diethyl ether, pathogenic to suckling but not to adult mice when given intracerebrally, intraperitoneally or subcutaneously, ...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1993-12-01 00:00:00
abstract：:Although most pathogenic rickettsiae are obligate intracellular parasites, it is clear that they are bacteria. As such, form and function in rickettsiae are closely similar to form and function found in their free-living counterparts. This review of rickettsiae as bacteria portrays the broad similarities of rickettsia...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1985-09-01 00:00:00
abstract：:Several putative cell surface receptors have been identified in the Hepatitis C virus (HCV) infection including CD81, low-density lipoprotein (LDL) receptor, scavenger receptor class B type I (SR-BI), and highly sulfated heparan sulfate (HS). In this study, we showed that the binding of soluble heparin to the envelope...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：2008-01-01 00:00:00
abstract：:A peculiar grouping of Creutzfeldt-Jacob disease (CJD) and of other organic presenile dementia (OPD) cases in a small, prevalently rural area in Czechoslovakia to the east of 19 degrees 35' E and to the north of 48 degrees 15' N was studied. Between August, 1972 and November, 1976, three histologically proven CJD case...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1978-03-01 00:00:00
abstract：:Human papillomavirus (HPV) E6 and E7 gene products play a central role in the induction of benign proliferation and malignant transformation by interacting with several cellular regulatory proteins such as p53, p16(INK4a), and nuclear factor kappaB (NF-kappaB). In this study, HPV DNA was detected by in situ hybridizat...
journal_title：Acta virologica
pub_type： 杂志文章
doi：10.4149/av_2009_01_43
更新日期：2009-01-01 00:00:00
abstract：:The present paper describes first full genome sequence of the Garlic virus D (GarV-D) from northern India with a genome size of 8425 bp long ssRNA. The infected leaves and bulbs of garlic variety Yamuna Safed (G-282) plants suspected for GarV-D infection were collected with the aim to identify contagion virus during M...
journal_title：Acta virologica
pub_type： 杂志文章
doi：10.4149/av_2020_405
更新日期：2020-01-01 00:00:00
abstract：:The chemical nature of BK virus receptors on human erythrocytes was investigated. Glycosidases, KIO4, proteases, phospholipases and other substances acting specifically on different structures of plasma membranes were used to ascertain the role of surface cell components in the interaction with BK virus. The results o...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1981-07-01 00:00:00
abstract：:One-step real-time RT-PCR assay was developed for quantification of the immediate-early (IE), namely IE1 and IE2 transcripts of Rat cytomegalovirus (RCMV), strain ALL-03 in rat embryonic fibroblast cells (REF). This in-house SYBR Green I based RT-PCR was shown to have higher amplification efficiency and detection limi...
journal_title：Acta virologica
pub_type： 杂志文章
doi：10.4149/av_2009_04_261
更新日期：2009-01-01 00:00:00
abstract：:Eight isolates of potato virus Y NTN strain (PVY-NTN) of different origin were studied by means of monoclonal antibodies (MAbs) in non-competitive and competitive enzyme-linked immunosorbent assay (ELISA), and by immunoblot analysis of the viral coat protein (CP). As the MAbs reacted with the denatured viral CP, their...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1999-12-01 00:00:00
abstract：:The presence of interferon and type A immunoglobulins (IgA) was followed up in the nasopharyngeal washings collected from volunteers immunized intranasally with an inactivated influenza vaccine [strain A/Rom 1/73 (H3N2)]. Interferon was detected 24 hours after vaccine administration, its incidence being similar to tha...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1975-05-01 00:00:00
abstract：:Immobilization stress (hypokinesis) in Balb/c mice may aggravate asymptomatic infection with Langat virus (strain TP-21) as evidenced by 4-fold increased lethality in comparison with control animals. The virus levels in the spleen and brain of stressed and infected mice and the in vitro yield of the virus in immunocom...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1990-05-01 00:00:00
abstract：:Variation of antibody level against West Nile (WN) virus depending on the season of birth was followed among 151 paired serum samples of healthy Pakistani persons in Karachi, collected twice in July and October, 1985. The persons born during the months between February and June had lower positive antibody rate and low...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1988-03-01 00:00:00
