Abstract：
:Cytotoxic T lymphocyte (CTL) activity of draining lymph node (DLN) cells isolated from BALB/c mice infected with ectromelia virus (EV) was examined using a fluorometric cell-mediated cytotoxicity (CMC) assay. Specific lysis of target cells A20 and EMT-6 primed with EV was demonstrated. The classical CD8+ cytolytic pathway dominated (72.7%) as compared to that of CD4+ (27.3%) in the cellular response during acute EV infection. Also an alternative method for determining CMC, employing a bisbenzamide dye for labelling target cells, is described. Coefficient variations of relative fluorescence were below 6%, that makes the method sensitive and reliable.
journal_name
Acta Viroljournal_title
Acta virologicaauthors
Toka FN,Niemiałtowski MG,Spohr de Faundez I,Gieryńska Msubject
Has Abstractpub_date
1996-11-01 00:00:00pages
239-44issue
5-6eissn
0001-723Xissn
1336-2305journal_volume
40pub_type
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