Abstract：
:An assay protocol based on exploiting the polymerase chain reaction (PCR) for the direct detection of influenza B virus in throat swabs is described. By the use of PCR with nested primers, it was possible to detect the virus in throat swabs. Dilution experiments showed that as little as 1 plaque forming unit of virus was sufficient for detecting the HA gene by the PCR. All throat swab samples from which influenza B virus had been isolated by conventional methods were also positive by the PCR method.
journal_name
Acta Viroljournal_title
Acta virologicaauthors
Yamada A,Imanishi Jsubject
Has Abstractpub_date
1992-05-01 00:00:00pages
320-5issue
3eissn
0001-723Xissn
1336-2305journal_volume
36pub_type
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