Partial replacement of serum with peptone and lactalbumin hydrolysate for the production of foot-and-mouth disease vaccine in BHK-21 cells.

Abstract:

:Two batches of experimental media were prepared with Difco-Peptone and Centron-Peptone in combination with lactalbumin hydrolysate (LAH) in Eagle's salts containing 3 amino acids, vitamins and 1% bovine serum. Both medium batches supported the growth of Razi BHK-21 cells in serial passages and the replication of foot-and-mouth disease (FMD) virus type "A". The infectivity and complement fixing antigen (CFU) titres of the virus were comparable with those in Eagle's medium. The protection indices "C" of the experimental batches of vaccine were highly satisfactory. The experimental media saved up to 90% of serum in comparison with Eagle's medium.

journal_name

Acta Virol

journal_title

Acta virologica

authors

Saha SN,Sen AK

subject

Has Abstract

pub_date

1989-08-01 00:00:00

pages

338-43

issue

4

eissn

0001-723X

issn

1336-2305

journal_volume

33

pub_type

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