Abstract:
:The H9N2 influenza virus has been frequently endemic in poultry, infected mammals and humans and has threatened public health. It is therefore imperative to understand the molecular mechanism enabling this virus to jump from avian to mammalian species. In this study, two H9N2 influenza viruses were isolated from the same region in eastern China but from different hosts; one was isolated from mink and named A/Mink/Shandong/WM01/2014(H9N2)(WM01), while the other was isolated from chicken and named A/Chicken/Shandong/LX830/2014(H9N2)(LX830). Sequencing and phylogenetic analysis showed that both H9N2 influenza viruses had similar genetic backgrounds. The results of infection in minks suggested that both viruses caused significant weight loss and pathological changes in the lungs. Mouse infection showed that LX830 was nonpathogenic in mice, but WM01 resulted in 25% mortality and pathological changes in the lungs, such as severe edema and diffused inflammation of the interalveolar septa. Comparison of the full genomes of both H9N2 influenza viruses showed 52-nucleotide-synonym mutations in 8 gene segments and 7-nucleotide-antonym mutations, resulting in 7 amino acid (AA) substitutions distributed in the PB1, PA, NA and M gene segments. None of these mutations did affect splicing of the M and NS gene segments at the nucleotide level or minor open reading frames (ORFs), such as PB1-F2 and PA-X. Phylogenetic analysis showed that both H9N2 influenza viruses belong to the prevalent epidemic genotype in Asia. Keywords: H9N2 influenza virus; chicken; minks; pathogenicity; phylogenetic.
journal_name
Acta Viroljournal_title
Acta virologicaauthors
Ren W,Zhang CH,Li G,Liu G,Shan H,Li Jdoi
10.4149/av_2020_109subject
Has Abstractpub_date
2020-01-01 00:00:00pages
67-77issue
1eissn
0001-723Xissn
1336-2305journal_volume
64pub_type
杂志文章相关文献
ACTA VIROLOGICA文献大全abstract::A strain of rickettsiae, designated Crimea-108, was isolated from ticks Dermacentor marginatus in the Crimea in 1977. Its immunobiological characteristics involve low pathogenicity for experimental animals, moderate infectivity for chick embryos, and antigenic relatedness to spotted fever group (SFG) rickettsiae (R. s...
journal_title:Acta virologica
pub_type: 杂志文章
doi:
更新日期:1993-12-01 00:00:00
abstract::The currently used Plowright's tissue culture rinderpest vaccine (RBOK strain) gives full protection and lifelong immunity, but it is highly thermolabile and requires maintenance of cold chain from vaccine production till delivery. Keeping in view the need for a thermostabile vaccine in tropical developing countries w...
journal_title:Acta virologica
pub_type: 杂志文章
doi:
更新日期:2001-01-01 00:00:00
abstract::The presence and the level of hepatitis B surface antigen (HBsAg)/IgM complexes were determined in 54 chronic HBsAg carriers in relation to receptors for polymerized human serum albumin (pHSA-R) tested by specific radioimmunoassay, and to hepatitis B virus-DNA polymerase (HBV-DNAp). HBsAg/IgM complexes, correlated sig...
journal_title:Acta virologica
pub_type: 杂志文章
doi:
更新日期:1987-03-01 00:00:00
abstract::Protective effects of different types of Q fever vaccines, namely untreated Coxiella burnetii phase I cells (Cb I) or Cb I cells treated with chloroform-methanol (CM) mixture (Cb I-CM) and of a Q fever chemovaccine obtained by trichloroacetic acid extraction (TCAE) from intact Cb I cells, were compared in mice and gui...
journal_title:Acta virologica
pub_type: 杂志文章
doi:
更新日期:1986-11-01 00:00:00
abstract::An indirect enzyme-linked immunosorbent assay (ELISA) was developed for the detection of antibodies against equine herpesvirus type 2 (EHV-2) in equine sera. The optimal conditions of antigen concentration, and serum and conjugate dilutions were established by chequerboard titrations. When the standard ELISA test was ...
journal_title:Acta virologica
pub_type: 杂志文章
doi:
更新日期:1987-11-01 00:00:00
abstract::To elucidate epidemiological interference between respiratory syncytial (RSV) and influenza viruses, the influence of influenza A (HlN1) virus on the growth of RSV was examined. Although RSV grew in MDCK cells, coinfection with influenza A virus led to a reduction of progeny RSV. The degree of growth interference depe...
journal_title:Acta virologica
pub_type: 杂志文章
doi:
更新日期:2000-04-01 00:00:00
abstract::Purified lipopolysaccharide-protein complex (LPS-PC) extracted by trichloroacetic acid from phase I Coxiella burnetii organisms induced in mice and rabbits fair levels of antibodies directed to antigen 1 and antigen 2, as detected by complement-fixation (CF), microagglutination (MA), opsonization-phagocytosis (OP) and...
journal_title:Acta virologica
pub_type: 杂志文章
doi:
更新日期:1978-07-01 00:00:00
abstract::Peanut stripe virus (PStV) is one of the most common viruses infecting peanut that causes great economic losses every year. The 3ʹ-terminal 1082 bp of 74 PStV isolates collected from 12 districts of Shandong province, China were sequenced. Their coat protein (CP) genes were 864 bp in length and shared identities of 98...
journal_title:Acta virologica
pub_type: 杂志文章
doi:10.4149/av_2011_02_123
更新日期:2011-01-01 00:00:00
abstract::Chronic infection of HeLa cells was induced by an attenuated variant of Japanese encephalitis (JE) virus (HeLa-K3 cell line). The chronic infection was characterized by alternating phases of degeneration and recovery of the cell monolayer. JE virus was regularly released into the medium of chronically infected cell cu...
journal_title:Acta virologica
pub_type: 杂志文章
doi:
更新日期:1980-12-01 00:00:00
abstract::Chromosomal DNAs isolated from eight individuals from the Slovak population and from lymphoblastoid Namalwa cells were analyzed for the presence of genes coding for three subvariants of human interferon-alpha 2 (IFN-alpha 2), namely a, b, and c. The respective genes are regarded allelic, because they differ in the cod...
journal_title:Acta virologica
pub_type: 杂志文章
doi:
更新日期:1994-04-01 00:00:00
abstract::Histological changes were studied in the central nervous system (CNS) of 58 Macaca rhesus monkeys, infected intracerebrally (i.c.) and subcutaneously (s.c.) with tick-borne encephalitis (TBE) virus. Subacute degenerative process in the CNS occurred in 7 monkeys on days 15-24 post-infection (p.i.), while chronic degene...
journal_title:Acta virologica
pub_type: 杂志文章
doi:
更新日期:1984-05-01 00:00:00
abstract::In order to determine the definitive importance of T- and B-lymphocytes in immunity to Rickettsia conorii, mice genetically deficient in T-cells, B-cells, or both T- and B-cells were infected experimentally. T-lymphocytes rather than humoral antibodies were crucial to rickettsial clearance and a reduced mortality rate...
journal_title:Acta virologica
pub_type: 杂志文章
doi:
更新日期:1984-11-01 00:00:00
abstract::Cytotoxic T lymphocyte (CTL) activity of draining lymph node (DLN) cells isolated from BALB/c mice infected with ectromelia virus (EV) was examined using a fluorometric cell-mediated cytotoxicity (CMC) assay. Specific lysis of target cells A20 and EMT-6 primed with EV was demonstrated. The classical CD8+ cytolytic pat...
journal_title:Acta virologica
pub_type: 杂志文章
doi:
更新日期:1996-11-01 00:00:00
abstract::Identification of structural protein relationships is likely to be important for virus assembly and anti-WSSV strategies. In this paper, VP90 of white spot syndrome virus (WSSV) was characterized. Temporal-transcription analysis showed that VP90 is expressed in the late stage of WSSV infection. Far-western and matrix-...
journal_title:Acta virologica
pub_type: 杂志文章
doi:10.4149/av_2012_01_57
更新日期:2012-01-01 00:00:00
abstract::Whitefly-transmitted viruses of the genus Begomovirus (the family Geminiviridae) have become a limiting factor for agricultural productivity in many warmer parts of the world. The economies of Pakistan and India have, since the early 1990s, suffered losses due to cotton leaf curl disease (CLCuD). The disease is caused...
journal_title:Acta virologica
pub_type: 杂志文章
doi:10.4149/av_2019_101
更新日期:2019-01-01 00:00:00
abstract::Cauliflower mosaic virus (CaMV) - a plant pararetrovirus that naturally causes diseases in Brassicaceae and Solanaceae plant hosts worldwide - has been detected by PCR for the first time in herbarium samples of Usnea sp. lichens. The virus's presence in these lichens did not result in any micro- or macromorphological ...
journal_title:Acta virologica
pub_type: 杂志文章
doi:10.4149/av_2016_02_196
更新日期:2016-06-01 00:00:00
abstract::The successive injection of non-immunomodulating doses of Tahyna virus (100 LD50) and non-immunomodulating doses of immunomodulating drugs, such as purified staphylococcal toxoid or glucosaminylmuramyldipeptide (Likopid), to mice were accompanied by a decrease in the IgM plaque-forming cell response to sheep red blood...
journal_title:Acta virologica
pub_type: 杂志文章
doi:
更新日期:1997-04-01 00:00:00
abstract::Acute gastroenteritis (AGE) is one of the most frequently occuring illnesses in children and adults worldwide. In February 2017, two AGE outbreaks occurred in two adjacent schools in Huzhou city, Zhenjiang province of China. We detected high percentages of recombinant norovirus GII.P16/GII.2 in one school and chicken ...
journal_title:Acta virologica
pub_type: 杂志文章
doi:10.4149/av_2019_312
更新日期:2019-01-01 00:00:00
abstract::Homologous interference in vitro is defined as the ability of primary viral infection to prevent secondary homologous superinfection. Non-cytopathic bovine viral diarrhea virus (ncp BVDV) has been classified according to the exaltation of Newcastle disease phenomenon (END) as END positive (E+) and END negative (E-) st...
journal_title:Acta virologica
pub_type: 杂志文章
doi:10.4149/av_2018_228
更新日期:2018-01-01 00:00:00
abstract::Human rotavirus strain 649 was isolated from a child with clinical picture of acute intestinal infection in the West European part of the U.S.S.R. This strain was adapted to MA-104 (rhesus monkey kidney) cells. By electrophoresis and haemagglutination it differed from the human rotavirus strain Wa and from the simian ...
journal_title:Acta virologica
pub_type: 杂志文章
doi:
更新日期:1991-01-01 00:00:00
abstract::14C-labelled Lassa virus was purified by isopycnic centrifugation and split to subvirion fractions. The purified virus was treated with nonionic detergents Nonidet P-40 (NP-40) and with octylglycoside. After ultracentrifugation in urographin density gradient, two subvirion fractions with buoyant density of 1.24-1.26 a...
journal_title:Acta virologica
pub_type: 杂志文章
doi:
更新日期:1989-03-01 00:00:00
abstract::The identification and genetic characterization of bovine viral diarrhea virus (BVDV) isolate 17237 detected in western Slovakia is described. The analysis of 5'-untranslated region (5'-UTR), autoprotease (Npro) gene, and structural genes (C, Erns, E1, E2) was carried out. The percentage of nucleotide and deduced amin...
journal_title:Acta virologica
pub_type: 杂志文章
doi:
更新日期:2008-01-01 00:00:00
abstract::Twenty three strains of simian viruses isolated from nasopharyngeal swabs and respiratory tissues of cynomolgus monkeys showing mild symptoms of respiratory disorders were classified into three groups by their biological, serological and morphological characteristics. Thirteen strains classified as group 1 were judged...
journal_title:Acta virologica
pub_type: 杂志文章
doi:
更新日期:1984-07-01 00:00:00
abstract::Over a 5-year observation period in a children-institution community, the severity of respiratory-syncytial (RS) virus infection varied within a broad range depending on biological properties of the circulating virus population. The type of virus population was characterized by a complex of biological features, namely...
journal_title:Acta virologica
pub_type: 杂志文章
doi:
更新日期:1985-07-01 00:00:00
abstract::Hemagglutinin-neuraminidase (HN) protein of Newcastle disease virus (NDV) plays a crucial role in induction of immune response against Newcastle disease infection. Mutation in residues 347 and 362 of HN linear antigenic site has been identified to be responsible for antigenic variations. Hence we studied antigenic dif...
journal_title:Acta virologica
pub_type: 杂志文章
doi:10.4149/av_2017_405
更新日期:2017-01-01 00:00:00
abstract::In this study, DNA vaccines consisting of vector IRES-gD expressing Herpes simplex virus 1 (HSV-1) glycoprotein D (gD) and vector IRES-gD-IL-2 coexpressing HSV-1 gD and interleukin-2 (IL-2), respectively, were constructed. After intramuscular inoculation, both vaccines induced in BALB/c mice antibodies as assayed by E...
journal_title:Acta virologica
pub_type: 杂志文章
doi:
更新日期:2006-01-01 00:00:00
abstract::Homologs of Bombyx mori nuclear polyhedrosis virus (BmNPV) Bm67 gene ORF67 have been found in the genome of all lepidopteran nuclear polyhedrosis viruses, but their function is still not very clear. In order to analyze it we employed a bacmid harboring the complete BmNPV genome including the Bm67 gene and expressing ...
journal_title:Acta virologica
pub_type: 杂志文章
doi:10.4149/av_2015_01_40
更新日期:2015-03-01 00:00:00
abstract::Hepatitis C virus (HCV) infection represents a serious public health problem worldwide. Development of new therapeutics against HCV has been hampered by the lack of a small-animal model. Until now, it has been unclear which host factors influence the HCV infection. It was known that human CD81 (hCD81) or low-density l...
journal_title:Acta virologica
pub_type: 杂志文章
doi:10.4149/av_2009_03_185
更新日期:2009-01-01 00:00:00
abstract::Structural proteins V1, V2 and V3 of Japanese encephalitis virus (JEV) were isolated and purified by means of polyacrylamide gel electrophoresis (PAGE) in the presence of sodium dodecyl sulfate (SDS) and extracted from the sliced gel. The purified and renatured V3 envelope protein was found to bind to receptors for JE...
journal_title:Acta virologica
pub_type: 杂志文章
doi:
更新日期:1982-09-01 00:00:00
abstract::Results of the direct immunofluorescence (IF) test in 152 clinical specimens (throat swabs) were compared with those of respiratory syncytial virus (RSV) isolation. The prevalence of RSV infections in the upper and lower respiratory tract was high especially in infants until 12 months of age. The average RSV isolation...
journal_title:Acta virologica
pub_type: 杂志文章
doi:
更新日期:1987-09-01 00:00:00