Abstract：
:Hemagglutinin-neuraminidase (HN) protein of Newcastle disease virus (NDV) plays a crucial role in induction of immune response against Newcastle disease infection. Mutation in residues 347 and 362 of HN linear antigenic site has been identified to be responsible for antigenic variations. Hence we studied antigenic difference between sub-genotype VIIj isolates and vaccine strains by the use of polyclonal serum against LaSota strain in hemagglutination inhibition test. Furthermore, epitope patterns of the isolates under study were analyzed and compared to HN sequences in GenBank. The results demonstrated that new Newcastle disease isolates (Mazandaran and Behshahr) had hemagglutination inhibition (HI) titer three and five, respectively while LaSota strain titer was eight. In addition, observation of sequences and epitope patterns revealed three unique amino acid substitutions (D144N, E347Q and G362K) in HN protein. E347Q and G362K mutations were located in neutralization antigenic site. Thus, we suggest that, these two novel amino acid substitutions in major linear epitope might be responsible for antigenic variation and decrease of HI activity.
journal_name
Acta Viroljournal_title
Acta virologicaauthors
Mayahi V,Esmaelizad Mdoi
10.4149/av_2017_405subject
Has Abstractpub_date
2017-01-01 00:00:00pages
438-444issue
4eissn
0001-723Xissn
1336-2305journal_volume
61pub_type
杂志文章相关文献
ACTA VIROLOGICA文献大全abstract：:Tick-borne encephalitis (TBE), a disease caused by tick-borne encephalitis virus (TBEV), represents a serious neural infection of humans in Europe and Asia. The main reservoir hosts of TBEV are ticks, rodents and insectivores, but domestic animals may also be infected with the virus. This review summarizes what is kno...
journal_title：Acta virologica
pub_type： 杂志文章
doi：10.4149/av_2020_212
更新日期：2020-01-01 00:00:00
abstract：:Immobilization stress (hypokinesis) in Balb/c mice may aggravate asymptomatic infection with Langat virus (strain TP-21) as evidenced by 4-fold increased lethality in comparison with control animals. The virus levels in the spleen and brain of stressed and infected mice and the in vitro yield of the virus in immunocom...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1990-05-01 00:00:00
abstract：:An Indian isolate of Goatpox virus (GTPV) was adapted and propagated in Vero cells for development of an attenuated virus. The virus was initially passaged in primary lamb testes cells and subsequently in Vero cells. At the 55th passage, the virus showed evidence of attenuation when tested for safety in seronegative g...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：2004-01-01 00:00:00
abstract：:For the quantitative assay of selected togaviruses, suspensions of BHK cells were inoculated with virus and grown in spinner cultures. At intervals, dependent on the growth characteristics of the viruses, about 10(3) cells were centrifuged on to microscope slides and then stained with fluorescent antibody. For rapid d...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1979-07-01 00:00:00
abstract：:Mouse polyclonal antibodies were prepared against broad bean stain virus (BBSV, Comovirus group) and its coat protein subunits, large (L) and small (S) protein. These subunits were less immunogenic than native virus. Antibodies against L protein (Anti-L) and native virus (Anti-BBSV) did not react in immunoblots with S...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1994-06-01 00:00:00
abstract：:Structural proteins V1, V2 and V3 of Japanese encephalitis virus (JEV) were isolated and purified by means of polyacrylamide gel electrophoresis (PAGE) in the presence of sodium dodecyl sulfate (SDS) and extracted from the sliced gel. The purified and renatured V3 envelope protein was found to bind to receptors for JE...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1982-09-01 00:00:00
abstract：:To elucidate epidemiological interference between respiratory syncytial (RSV) and influenza viruses, the influence of influenza A (HlN1) virus on the growth of RSV was examined. Although RSV grew in MDCK cells, coinfection with influenza A virus led to a reduction of progeny RSV. The degree of growth interference depe...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：2000-04-01 00:00:00
abstract：:A mathematical model of the pathogenesis of experimental Mengo virus infection in mice has been developed and fitted using kinetic data of both virus multiplication in different organs and mortality. The behaviour of the model proved to be bistable. In contrast to the widely accepted hypothesis that an acutely virus-i...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1987-08-01 00:00:00
abstract：:Cell-mediated reactions in measles cases, in direct contacts and healthy children were tested. Indices of phytohaemagglutinin-stimulated blastic transformation and leukocyte migration inhibition were evaluated. Positive reactions were found in the first and second weeks after manifestation of clinical symptoms. The ap...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1980-03-01 00:00:00
abstract：:Four isolates of measles virus (Gag, Il, Buk and Shed) were obtained from suspensions of mononuclear cells from patients at the active stage of the disease. Vero cells were used for the virus isolation. All the isolates caused in the infected cell culture the appearance of symplasts of differently sized, star- or spin...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1992-12-01 00:00:00
abstract：:CVI988/Rispens strain of Marek's disease virus type I (MDV-1) is widely used as efficient vaccine to control Marek's disease (MD) in chicken flocks. Similarly to other live MD vaccine viruses it is propagated in freshly prepared chicken embryo fibroblasts (CEF). In this study, MDV-1 CVI988/Rispens strain was adapted t...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：2001-04-01 00:00:00
abstract：:The densities of purified herpes simplex virus (HSV) particles prepared from infected rabbit lung (ZP) and baby hamster kidney (BHK-21) cells were investigated in potassium tartrate (PT) and potassium citrate (PC) density gradients. Virions obtained from ZP cells exhibited a higher density than those from BHK-21 cells...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1976-12-01 00:00:00
abstract：:Evidence has been obtained indicating the association of certain biological properties of Rickettsia prowazekii with their capacity for persistence. Highly virulent rickettsial cultures inducing increased levels of complement-fixing antibodies were shown to cause a high percentage of rickettsial carriers in infected c...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1983-11-01 00:00:00
abstract：:Two different radio-labeled nucleic acid probes, prepared from reverse transcription-polymerase chain reaction (RT-PCR) amplified variable region of VP2 and VP1 gene sequences of a highly virulent infectious bursal disease virus (IBDV), were tested for their ability to detect field isolates of IBDV directly in clinica...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：2000-10-01 00:00:00
abstract：:Antigenic relationships between the four subgroups of the Kemerovo serogroup of tick-borne orbiviruses were examined. Kemerovo (KEM subgroup), Broadhaven (BRD) and Wexford (WEX) [Great Island (GI) sub-group], Chenuda (CNU sub-group), and Wad Medani (WM sub-group) viruses cross-reacted in immunofluorescence tests. Comp...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1989-01-01 00:00:00
abstract：:The interferon system represents one of the components of the first line defence against influenza virus infection. Interferon omega (IFN-ω) is antigenetically different from IFN-α and IFN-β and can affect patients who are resistant to these IFNs. To improve the biological characterization of IFN-ω, we compared its ac...
journal_title：Acta virologica
pub_type： 杂志文章
doi：10.4149/av_2015_04_413
更新日期：2015-12-01 00:00:00
abstract：:In vivo phagocytosis activity of macrophages (PAM) was temporarily suppressed in mice by application of a suspension of microscopic from particles. As demonstrated, a reversible block of 70% of PAM was accompanied by a marked increase of the lethality during the acute tick-borne encephalitis (TBE) virus infection. Asy...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1985-05-01 00:00:00
abstract：:While the main interest in the pathogenesis of herpes simplex virus (HSV) in the sixties had been focussed on acute infections, in the seventies latent infection has become the main foal of investigation. Despite of overwhelming literature, the HSV persistence has remained a continuing problem from the practical as we...
journal_title：Acta virologica
pub_type： 杂志文章,评审
doi：
更新日期：1983-09-01 00:00:00
abstract：:Nine samples of diseased Malvastrum coromandelianum plants collected from the fields in Yunnan province of China were found to be infected with Malvastrum yellow vein Yunnan virus (MYVYNV), when tested by PCR using specific primers. The results of PCR and Southern blot analysis showed that only 4 samples out of 9 were...
journal_title：Acta virologica
pub_type： 杂志文章
doi：10.4149/av_2010_01_21
更新日期：2010-01-01 00:00:00
abstract：:Human choriocarcinoma cells of the JAR line, with no demonstrable surface CD4 receptor were infected with human immunodeficiency virus type 1 (HIV-1), strain RF. Primer-directed enzymatic DNA amplification (polymerase chain reaction, PCR) detected the presence of viral DNA when the cultures were investigated at day 5 ...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1991-09-01 00:00:00
abstract：:Substance P, a neuropeptide of the tachykinin group, inhibits measles virus replication in cell culture and partially blocks viral fusion activity assayed in the haemolysis system. The ID50 for the inhibition of measles virus single-cycle replication is 0.6 mumol/l, and the effect is fully reversible. The antiviral ac...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1986-09-01 00:00:00
abstract：:Tick-borne viruses are causative agents of several important human diseases. Tick-borne encephalitis virus (TBEV) is the most prominent representative considered medically to be the most important arbovirus (arthropod-borne virus) in Europe and northern Asia. Tick-borne virus transmission cycles are determined by the ...
journal_title：Acta virologica
pub_type： 杂志文章,评审
doi：10.4149/av_2013_02_123
更新日期：2013-01-01 00:00:00
abstract：:The rate of the synthesis of tick-borne encephalitis (TBE) virus-specified proteins at high multiplicity of infection (MOI of 100 PFU per cell) was the highest by 8-14 hr post-infection (p.i.) as compared to the lower MOI of 4 PFU per cell (14 to 17 hr p.i.). The first virus-specific proteins appeared in cells from 2 ...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1986-07-01 00:00:00
abstract：:The results of the study on the combined antiviral activity of the SHS-174 preparation (a lyophylized infusion from three higher plants) and three amantadine derivatives (rimantadine, amantadine glucuronide and its derivative) are presented. The antiviral effect of the drugs on the reproduction of influenza virus stra...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1993-08-01 00:00:00
abstract：:Overweight and obesity is becoming widespread enough to generate an acceptable and misleading social status. By 2030, in the USA up to 86 % of adults will be overweight or obese. Some selected statistical data based on the body mass index (BMI) indicated that overweight was not associated with increased mortality, thi...
journal_title：Acta virologica
pub_type： 杂志文章
doi：10.4149/av_2014_03_253
更新日期：2014-01-01 00:00:00
abstract：:The generally accepted concept that the replication of Rous sarcoma virus (RSV) is dependent on host cell DNA synthesis was reexamined. As the host we used terminally differentiated myotubes (MT), in which no cellular DNA synthesis is observed. As an extension of our previous study which indicated that RSV-infected MT...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1995-09-01 00:00:00
abstract：:Interferon (IFN) was induced in chicken peripheral blood leukocytes by exposure to Concanavalin A (Con A). It has reached a maximum level 96 hr after stimulation with the optimum dose of 10 micrograms/ml. The IFN was partially purified by chromatography on controlled-pore glass adsorbent and was characterized as a fai...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1986-03-01 00:00:00
abstract：:The plaque size and distribution of prototype La Crosse (LAC) and Jamestown Canyon (JC) viruses were investigated in Vero cell cultures. The effect of serial passage of the viruses in their natural vertebrate hosts - the chipmunk and grey squirrel for LAC and the white-tailed deer for JC virus - was studied. Prototype...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1975-07-01 00:00:00
abstract：:Invasiveness of Shigella flexneri M90T in HeLa cells was significantly increased when cells were preinfected with poliovirus 1, coxsackievirus B3 and echovirus 6. This effect was dependent on the dose of virus used, evident at early stages of viral infection and lasted hours before the appearance of a cytopathic effec...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1992-10-01 00:00:00
abstract：:Two batches of experimental media were prepared with Difco-Peptone and Centron-Peptone in combination with lactalbumin hydrolysate (LAH) in Eagle's salts containing 3 amino acids, vitamins and 1% bovine serum. Both medium batches supported the growth of Razi BHK-21 cells in serial passages and the replication of foot-...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1989-08-01 00:00:00
