Abstract：
:The generally accepted concept that the replication of Rous sarcoma virus (RSV) is dependent on host cell DNA synthesis was reexamined. As the host we used terminally differentiated myotubes (MT), in which no cellular DNA synthesis is observed. As an extension of our previous study which indicated that RSV-infected MT produce various virus components, we examined viral particles produced by infected MT. Electron microscopy showed presence of viral particles released from infected MT. Immunoprecipitation analysis revealed that these particles contained an equal amount of the gag but a decreased amount of the env proteins as compared with the particles from infected chicken embryo fibroblasts (CEF). Consequently, viral particles from infected MT had an infectivity only 6% of that of particles from infected CEF cells. In a parallel experiment, we microinjected molecularly cloned RSV DNA into MT. In contrast to the infection mediated by viral particles, both MT and CEF cells produced the same amount of infectious particles when microinjected with viral DNA. We conclude that RSV replicates in the complete absence of host DNA synthesis, though infectivity of the progeny virus depends on the initial condition of the infection.
journal_name
Acta Viroljournal_title
Acta virologicaauthors
Park HT,Hara H,Fujihara M,Hsia KJ,Tanaka A,Furuse T,Iwasaki Y,Kaji Asubject
Has Abstractpub_date
1995-09-01 00:00:00pages
197-204issue
4eissn
0001-723Xissn
1336-2305journal_volume
39pub_type
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