Development of probes for differentiation of infectious bursal disease virus strains of various virulence by dot-blot hybridization.

Abstract:

:Two different radio-labeled nucleic acid probes, prepared from reverse transcription-polymerase chain reaction (RT-PCR) amplified variable region of VP2 and VP1 gene sequences of a highly virulent infectious bursal disease virus (IBDV), were tested for their ability to detect field isolates of IBDV directly in clinical bursal tissue specimens and vaccine strains of IBDV in tissue cultures. The VP2 gene probe was able to detect both field isolates and vaccine strains of IBDV under high as well as low stringency while the VP1 gene probe could differentiate under high stringency field isolates from vaccine strains, hybridizing only with RNA of field isolates. The sensitivity of both the probes was found to be 4 ng of purified viral RNA.

journal_name

Acta Virol

journal_title

Acta virologica

authors

Katari RS,Tiwari AK,Butchaiah G,Kataria JM

subject

Has Abstract

pub_date

2000-10-01 00:00:00

pages

259-63

issue

5

eissn

0001-723X

issn

1336-2305

journal_volume

44

pub_type

杂志文章
  • Effects of edeine, hygromycin B and alpha-Sarcin on influenza virus reproduction.

    abstract::The effects of edeine, hygromycin B and alpha-sarcin on the synthesis of virus-specific proteins and formation of infectious virions was studied in cells infected with fowl plague virus (FPV). The manifestation of the antiviral effect of edeine depended on the peculiarities of the FPV strains and the host-cell systems...

    journal_title:Acta virologica

    pub_type: 杂志文章

    doi:

    authors: Ghendon YuZ,Klimov AI

    更新日期:1981-05-01 00:00:00

  • Virion proteins and the perspectives of gene manipulations in vaccine preparation.

    abstract::The achievements and perspectives of genetic manipulations are described aiming at preparation of first generation subunit vaccines based on the synthesis in bacterial and eukaryotic cells of full-sized virion proteins expressing the main antigenic determinants. The preparation of such vaccines in bacterial cells seem...

    journal_title:Acta virologica

    pub_type: 杂志文章,评审

    doi:

    authors: Tickhonenko TI

    更新日期:1985-05-01 00:00:00

  • Antiviral activity of hypothiocyanite produced by lactoperoxidase against influenza A and B viruses and mode of its antiviral action.

    abstract::Hypothiocyanite (OSCN-) is a natural component of human saliva and is produced by the lactoperoxidase (LPO)/thiocyanate/hydrogen peroxide (H2O2) system. OSCN- has been previously shown to exhibit antiviral activity against influenza viruses (IFV) A/H1N1/2009 and A/H1N2/2009 in vitro as well as antimicrobial and antifu...

    journal_title:Acta virologica

    pub_type: 杂志文章

    doi:10.4149/av_2018_408

    authors: Sugita C,Shin K,Wakabayashi H,Tsuhako R,Yoshida H,Watanabe W,Kurokawa M

    更新日期:2018-01-01 00:00:00

  • Quantitative and rapid interference of bovine viral diarrhea virus BVDV/END- and BVDV/END+ strains.

    abstract::Homologous interference in vitro is defined as the ability of primary viral infection to prevent secondary homologous superinfection. Non-cytopathic bovine viral diarrhea virus (ncp BVDV) has been classified according to the exaltation of Newcastle disease phenomenon (END) as END positive (E+) and END negative (E-) st...

    journal_title:Acta virologica

    pub_type: 杂志文章

    doi:10.4149/av_2018_228

    authors: Muhsen M,Lee P

    更新日期:2018-01-01 00:00:00

  • Eperythrozoon--a lesson for arbovirologists.

    abstract::Eperythrozoon was detected in suckling mice inoculated with a suspension of nymphal Ixodes ricinus ticks. The organism was filterable through 220 nm Millipore membranes, moderately sensitive to diethyl ether, pathogenic to suckling but not to adult mice when given intracerebrally, intraperitoneally or subcutaneously, ...

    journal_title:Acta virologica

    pub_type: 杂志文章

    doi:

    authors: Hubálek Z,Juricová Z,Halouzka J

    更新日期:1993-12-01 00:00:00

  • Molecular detection and characterization of phytoplasma in association with begomovirus in eggplant.

    abstract::The samples from eggplants showing mixed symptoms of little leaf and mosaic were collected from two districts (Mirzapur and Varanasi) of Uttar Pradesh, India. The total nucleic acid extracted from these samples was amplified by PCR using universal 16S rRNA primers specific to phytoplasma and primers specific to DNA-A-...

    journal_title:Acta virologica

    pub_type: 杂志文章

    doi:10.4149/av_2018_218

    authors: Venkataravanappa V,Prasanna HC,Lakshminarayana CN,Reddy MK

    更新日期:2018-01-01 00:00:00

  • The antisense 5' end of the V2 gene confers enhanced resistance against the monopartite begomovirus cotton leaf curl Kokhran virus-Burewala strain.

    abstract::Whitefly-transmitted viruses of the genus Begomovirus (the family Geminiviridae) have become a limiting factor for agricultural productivity in many warmer parts of the world. The economies of Pakistan and India have, since the early 1990s, suffered losses due to cotton leaf curl disease (CLCuD). The disease is caused...

    journal_title:Acta virologica

    pub_type: 杂志文章

    doi:10.4149/av_2019_101

    authors: Ali I,Khurshid M,Iqbal Z,Shafiq M,Amin I,Mansoor S,Briddon RW

    更新日期:2019-01-01 00:00:00

  • Genetic variety of bovine viral diarrhea virus 1 strains isolated from sheep and goats in India.

    abstract:UNLABELLED:Antigenic and genetic typing of pestiviruses isolated from Indian sheep and goats was carried out. Testing of 1777 sheep and 1026 goat blood samples collected between 2004 and 2008 resulted in isolation of twelve pestiviruses, seven from sheep and five from goats. All of them were antigenically typed as bovi...

    journal_title:Acta virologica

    pub_type: 杂志文章

    doi:10.4149/av_2012_03_209

    authors: Mishra N,Pitale SS,Rajukumar K,Prakash A,Behera SP,Nema RK,Dubey SC

    更新日期:2012-01-01 00:00:00

  • Antiviral effect of a derivative of isonicotinic acid enisamium iodide (FAV00A) against influenza virus.

    abstract::With only a single class of antiviral drugs existing for treatment of influenza (neuraminidase inhibitors), the search for novel effective compounds is urgently needed. We evaluated a low molecular mass compound, enisamium iodide (FAV00A), against influenza virus infections in primary differentiated normal human bronc...

    journal_title:Acta virologica

    pub_type: 杂志文章

    doi:10.4149/av_2018_211

    authors: Cocking D,Cinatl J,Boltz DA,Peng X,Johnson W,Muzzio M,Syarkevych O,Kostyuk G,Goy A,Mueller L,Margitich VI

    更新日期:2018-01-01 00:00:00

  • Protection of rabbits against lapinized rinderpest virus with purified envelope glycoproteins of peste-des-petits-ruminants and rinderpest viruses.

    abstract::Haemagglutinin (HA) and fusion (F) proteins of peste-des-petits-ruminants virus (PPRV) and rinderpest virus (RPV) were purified by immunoaffinity chromatography. The purified proteins were characterized by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate (SDS-PAGE). Rabbit hyperimmune sera ...

    journal_title:Acta virologica

    pub_type: 杂志文章

    doi:

    authors: Devireddy LR,Raghavan R,Ramachandran S,Subbarao SM

    更新日期:1998-11-01 00:00:00

  • Analysis of hepatitis C virus isolates using molecular and serological typing methods.

    abstract::This study comprised 100 persons with antibodies to hepatitis C virus (HCV), including 77 intravenous drug users (IVDUs). They were tested with serological HCV typing assays (Murex HCV serotyping 1-6 assay; Chiron RIBA HCV Serotyping SIA). Patients with a positive polymerase chain reaction (PCR) for HCV (n = 66) were ...

    journal_title:Acta virologica

    pub_type: 杂志文章

    doi:

    authors: Cretel E,Gallian P,Obadia Y,Rousseau S,de Micco P,de Lamballerie X

    更新日期:1997-10-01 00:00:00

  • Activities of a lagging DNA strand synthesis of nucleoprotein complexes harboring an extrachromosomal DNA closely related to avian myeloblastosis virus core-bound DNA.

    abstract::Nucleoprotein (NP) complexes constituting the material of the postmicrosomal sediment (POMS) and its three basic components (A, B, C) (Ríman and Sulová, 1997a), harboring an extrachromosomal DNA closely related to AMV DNA were found to possess DNA- and RNA-synthesizing activities (SAs) reflecting the ability of this m...

    journal_title:Acta virologica

    pub_type: 杂志文章

    doi:

    authors: Ríman J,Sulová A

    更新日期:1997-08-01 00:00:00

  • Effect of repeated Ribavirin treatment on grapevine viruses.

    abstract::The effect of Ribavirin treatment for the chemotherapy of several grapevine viruses was evaluated. Four grapevine cultivars were repeatedly treated with Ribavirin in two different concentrations and with three different lengths of treatment. Repeating the Ribavirin treatment always had a significant effect on the numb...

    journal_title:Acta virologica

    pub_type: 杂志文章

    doi:10.4149/av_2016_04_400

    authors: Komínek P,Komínková M,Jandová B

    更新日期:2016-01-01 00:00:00

  • Two biologically distinct isolates of Zucchini yellow mosaic virus lack seed transmissibility in cucumber.

    abstract::The seed transmission of the Zucchini yellow mosaic virus (ZYMV) was studied in cucumber using two isolates unrelated in their biological characteristics. Although the virus could be readily detected in mature seeds harvested from infected cucumbers, the seedlings obtained from infected germinated seeds tested negativ...

    journal_title:Acta virologica

    pub_type: 杂志文章

    doi:

    authors: Glasa M,Kollerova E

    更新日期:2007-01-01 00:00:00

  • Localization of human recombinant adenoviral vectors to the mitochondria following transduction of human cell lines.

    abstract::Recently, mitochondria have been shown to have a vital role in the innate immune response to viral infection. In response, viruses such as adenovirus, have developed mechanisms to alter mitochondrial function through direct or indirect interaction with the mitochondria. The interaction of human recombinant adenoviral ...

    journal_title:Acta virologica

    pub_type: 杂志文章

    doi:10.4149/av_2019_114

    authors: Morrison BJ,Abu-Asab M,Morris JC,Steel JC

    更新日期:2019-01-01 00:00:00

  • Persistence of tick-borne encephalitis virus in monkeys. VI. Pathomorphology of chronic infection in central nervous system.

    abstract::Histological changes were studied in the central nervous system (CNS) of 58 Macaca rhesus monkeys, infected intracerebrally (i.c.) and subcutaneously (s.c.) with tick-borne encephalitis (TBE) virus. Subacute degenerative process in the CNS occurred in 7 monkeys on days 15-24 post-infection (p.i.), while chronic degene...

    journal_title:Acta virologica

    pub_type: 杂志文章

    doi:

    authors: Frolova MP,Pogodina VV

    更新日期:1984-05-01 00:00:00

  • Effect of 2-deoxy-D-glucose on the binding of preimmune and immune sera to herpes simplex virus-infected cells as measured by quantitative immunofluorescence.

    abstract::The binding of immune and preimmune rabbit sera to fixed VERO cells infected with herpes simplex virus type 1 (HSV 1) was measured by quantitative immunofluorescence. False positive binding of the preimmune serum (PS) occurred from 8 hr post infection (p.i.), while the immune serum (IS) revealed positive fluorescence ...

    journal_title:Acta virologica

    pub_type: 杂志文章

    doi:

    authors: Rajcáni J,Hulíková Z,Mucha V

    更新日期:1979-09-01 00:00:00

  • Sepcific immunofluorescent IgG, IgM, and IgA antibodies in lymphocytic choriomeningitis.

    abstract::In postnatally acquired lymphocytic choriomeningitis (LCM), specific immunofluorescent (IF) IgM antibody was found nearly in all the examined sera from 23 patients for 6-8 months after onset of the disease, IgA antibody in two-thirds of the patients and only during the first 5 weeks of the disease. In the first weeks,...

    journal_title:Acta virologica

    pub_type: 杂志文章

    doi:

    authors: Sheinbergas MM,Verikene VV,Maslinskas VY,Lyubetsky VB

    更新日期:1978-05-01 00:00:00

  • Effect of cellular inhibitors on the infection of various susceptible cells with vesicular stomatitis virus.

    abstract::The effect of some cellular function inhibitors on adsorption and successive events of penetration of vesicular stomatitis virus to phylogenetically unrelated permissive cells was investigated. Treatment of HeLa, CER, EPC and Aedes albopictus cells with colcemide and cytochalasin D which affect cytoskeleton organizati...

    journal_title:Acta virologica

    pub_type: 杂志文章

    doi:

    authors: Superti F,Seganti L,Orsi N

    更新日期:1988-11-01 00:00:00

  • Effects of monoparacoumarylputrescinium chloride on the hypersensitive reaction of Gomphrena globosa leaves to tomato bushy stunt virus.

    abstract::Monoparacoumarylputrescinium chloride (pCPH), supplied to detached leaves of Gomphrena globosa via the petiole, induced interference with tomato bushy stunt virus infection by reducing the number and size of the necrotic local lesions. The phenolic compound neither inactivated directly the virus in vitro, nor induced ...

    journal_title:Acta virologica

    pub_type: 杂志文章

    doi:

    authors: Pennazio S,Dellamonica G,Redolfi P

    更新日期:1981-03-01 00:00:00

  • Coxsackievirus infection of mice. I. Viral kinetics and histopathological changes in mice experimentally infected with coxsackieviruses B3 and B4 by oral route.

    abstract::We followed the viral kinetics and histopathological changes in different organs of immunocompetent mice infected orally with coxsackieviruses B3 (CVB3) Nancy strain and B4 (CVB4) JVB strain separately. The viruses used were not adapted to mouse organs. In the acute phase of infection, the viral kinetics indicated vir...

    journal_title:Acta virologica

    pub_type: 杂志文章

    doi:

    authors: Bopegamage S,Borsanyiová M,Vargová A,Petrovicová A,Benkovicová M,Gomolcák P

    更新日期:2003-01-01 00:00:00

  • Experimental double infection with Coxiella burnetii and tick-borne encephalitis virus in Dermacentor reticulatus ticks.

    abstract::Experimental parenteral inoculation of Dermacentor reticulatus ticks with the rickettsia Coxiella burnetii (C.b.) and tick-borne encephalitis (TBE) virus resulted in a generalized rickettsial and viral infection, irrespective of whether the agents were given simultaneously or by a 7 days interval apart. Both agents mu...

    journal_title:Acta virologica

    pub_type: 杂志文章

    doi:

    authors: Rehácek J,Kovácová E,Ciampor F,Gresíková M,Tarasevich IV

    更新日期:1987-01-01 00:00:00

  • Effect of maternal varicella-zoster virus infection on the outcome of pregnancy and the analysis of transplacental virus transmission.

    abstract::In result of 20 pregnancies complicated by varicella 11 healthy and 6 defective offspring were born, 2 pregnancies were aborted by the physician and one pregnancy terminated by stillbirth of 3 siblings. Laboratory investigation of 16 pregnancies has shown that transplacental transfer of varicella-zoster (VZ) virus did...

    journal_title:Acta virologica

    pub_type: 杂志文章

    doi:

    authors: Trlifajová J,Benda R,Benes C

    更新日期:1986-05-01 00:00:00

  • A rickettsia-like organism showing positive immunofluorescence with antisera to Coxiella burnetii in Haemaphysalis inermis ticks.

    abstract::A rickettsia-like organism (RLO) was detected in the oocytes of Haemaphysalis inermis ticks. The RLOs were about 5 microns long bent rods with a definite inner structure. They were Gram-negative and could be visualized by Giemsa but not by Gimenez staining. Attempts to cultivate the RLO in chick embryo yolk sacs, vari...

    journal_title:Acta virologica

    pub_type: 杂志文章

    doi:

    authors: Kovácová E,Rehácek J,Ciampor F

    更新日期:1981-09-01 00:00:00

  • Inactivation of tobacco mosaic virus using gamma irradiation and its potential modes of action.

    abstract::Gamma irradiation is a non-thermal processing technique used to disinfect harmful microorganisms in agriculture. This technology has been shown to be an effective method to control bacterial and fungal plant pathogens. However, its effect on viral plant pathogen is less understood. Gamma irradiation was evaluated for ...

    journal_title:Acta virologica

    pub_type: 杂志文章

    doi:10.4149/av_2017_02_14

    authors: Jeong RD,Choi HS

    更新日期:2017-01-01 00:00:00

  • Sequence and phylogenetic analyses of five low pathogenic avian influenza H5N2 viruses isolated in China.

    abstract::Five H5N2 avian influenza viruses (AIVs) were isolated in Xianghai National Nature Reserve, Jilin Province, China in October 2011. They were all identified as low pathogenic AIVs (LPAIVs) based on their deduced amino acid sequences at the cleavage site of HA protein. Phylogenetic analysis revealed that all gene segmen...

    journal_title:Acta virologica

    pub_type: 杂志文章

    doi:10.4149/av_2015_02_140

    authors: Yang S,Chen G,Sun J,Li F,Hua HC

    更新日期:2015-06-01 00:00:00

  • Ts mutations in the genome of cold-adapted variants of human influenza virus A/Krasnodar/101/59 (H2N2) at different passage levels and their reproduction in lungs of hamsters.

    abstract::Human influenza virus A/Krasnodar/101/59 (H2N2) was passaged in chick fibroblast cultures in the presence of trypsin at suboptimal temperature. The virus which underwent 16 passages at 28 degrees C possessed cold-adapted (ca) and temperature sensitive (ts) phenotypes and formed larger plaques at the optimal temperatur...

    journal_title:Acta virologica

    pub_type: 杂志文章

    doi:

    authors: Panzig B,Döhner L,Lisovskaya KB,Ghendon YZ

    更新日期:1984-09-01 00:00:00

  • First confirmed sheep scrapie with A136R154Q171 genotype in Slovakia.

    abstract::The first confirmed evidence of scrapie in Slovakia was demonstrated in one sheep of the autochthonous Merino breed from the southeastern part of the country. The reported scrapie was diagnosed during compulsory transmissible spongiform encephalitis (TSE) screening of sheep over 9 months of age assigned for consumptio...

    journal_title:Acta virologica

    pub_type: 杂志文章

    doi:

    authors: Matúsková M,Csóková N,Filipcík P,Hanusovská E,Bíres J,Cabadaj R,Kontsek P,Novák M

    更新日期:2003-01-01 00:00:00

  • Eradication trials of tobacco mosaic virus using chemical drugs.

    abstract:KEYWORDS:virus sanitation; in vitro explants; antiviral mechanism; virus longevity. ...

    journal_title:Acta virologica

    pub_type: 杂志文章

    doi:10.4149/av_2012_02_159

    authors: Luvisi A,Panattoni A,Triolo E

    更新日期:2012-01-01 00:00:00

  • Detection by immunofluorescence of avian myeloblastosis virus reverse transcriptase.

    abstract::Peripheral blood cells of avian myeloblastosis virus (AMV-(infected chickens were examined at various intervals post infection by immunofluorescence. AMV revertase was identified in pro- and myeloblasts; it was localized mainly in the perinuclear zone or throughout the cytoplasm. No revertase was found in erythrocytes...

    journal_title:Acta virologica

    pub_type: 杂志文章

    doi:

    authors: Karmysheva VY,Graevskaya NA,Sito AF,Sushko LN

    更新日期:1976-08-01 00:00:00