Abstract:
:Two different radio-labeled nucleic acid probes, prepared from reverse transcription-polymerase chain reaction (RT-PCR) amplified variable region of VP2 and VP1 gene sequences of a highly virulent infectious bursal disease virus (IBDV), were tested for their ability to detect field isolates of IBDV directly in clinical bursal tissue specimens and vaccine strains of IBDV in tissue cultures. The VP2 gene probe was able to detect both field isolates and vaccine strains of IBDV under high as well as low stringency while the VP1 gene probe could differentiate under high stringency field isolates from vaccine strains, hybridizing only with RNA of field isolates. The sensitivity of both the probes was found to be 4 ng of purified viral RNA.
journal_name
Acta Viroljournal_title
Acta virologicaauthors
Katari RS,Tiwari AK,Butchaiah G,Kataria JMsubject
Has Abstractpub_date
2000-10-01 00:00:00pages
259-63issue
5eissn
0001-723Xissn
1336-2305journal_volume
44pub_type
杂志文章相关文献
ACTA VIROLOGICA文献大全abstract::The effects of edeine, hygromycin B and alpha-sarcin on the synthesis of virus-specific proteins and formation of infectious virions was studied in cells infected with fowl plague virus (FPV). The manifestation of the antiviral effect of edeine depended on the peculiarities of the FPV strains and the host-cell systems...
journal_title:Acta virologica
pub_type: 杂志文章
doi:
更新日期:1981-05-01 00:00:00
abstract::The achievements and perspectives of genetic manipulations are described aiming at preparation of first generation subunit vaccines based on the synthesis in bacterial and eukaryotic cells of full-sized virion proteins expressing the main antigenic determinants. The preparation of such vaccines in bacterial cells seem...
journal_title:Acta virologica
pub_type: 杂志文章,评审
doi:
更新日期:1985-05-01 00:00:00
abstract::Hypothiocyanite (OSCN-) is a natural component of human saliva and is produced by the lactoperoxidase (LPO)/thiocyanate/hydrogen peroxide (H2O2) system. OSCN- has been previously shown to exhibit antiviral activity against influenza viruses (IFV) A/H1N1/2009 and A/H1N2/2009 in vitro as well as antimicrobial and antifu...
journal_title:Acta virologica
pub_type: 杂志文章
doi:10.4149/av_2018_408
更新日期:2018-01-01 00:00:00
abstract::Homologous interference in vitro is defined as the ability of primary viral infection to prevent secondary homologous superinfection. Non-cytopathic bovine viral diarrhea virus (ncp BVDV) has been classified according to the exaltation of Newcastle disease phenomenon (END) as END positive (E+) and END negative (E-) st...
journal_title:Acta virologica
pub_type: 杂志文章
doi:10.4149/av_2018_228
更新日期:2018-01-01 00:00:00
abstract::Eperythrozoon was detected in suckling mice inoculated with a suspension of nymphal Ixodes ricinus ticks. The organism was filterable through 220 nm Millipore membranes, moderately sensitive to diethyl ether, pathogenic to suckling but not to adult mice when given intracerebrally, intraperitoneally or subcutaneously, ...
journal_title:Acta virologica
pub_type: 杂志文章
doi:
更新日期:1993-12-01 00:00:00
abstract::The samples from eggplants showing mixed symptoms of little leaf and mosaic were collected from two districts (Mirzapur and Varanasi) of Uttar Pradesh, India. The total nucleic acid extracted from these samples was amplified by PCR using universal 16S rRNA primers specific to phytoplasma and primers specific to DNA-A-...
journal_title:Acta virologica
pub_type: 杂志文章
doi:10.4149/av_2018_218
更新日期:2018-01-01 00:00:00
abstract::Whitefly-transmitted viruses of the genus Begomovirus (the family Geminiviridae) have become a limiting factor for agricultural productivity in many warmer parts of the world. The economies of Pakistan and India have, since the early 1990s, suffered losses due to cotton leaf curl disease (CLCuD). The disease is caused...
journal_title:Acta virologica
pub_type: 杂志文章
doi:10.4149/av_2019_101
更新日期:2019-01-01 00:00:00
abstract:UNLABELLED:Antigenic and genetic typing of pestiviruses isolated from Indian sheep and goats was carried out. Testing of 1777 sheep and 1026 goat blood samples collected between 2004 and 2008 resulted in isolation of twelve pestiviruses, seven from sheep and five from goats. All of them were antigenically typed as bovi...
journal_title:Acta virologica
pub_type: 杂志文章
doi:10.4149/av_2012_03_209
更新日期:2012-01-01 00:00:00
abstract::With only a single class of antiviral drugs existing for treatment of influenza (neuraminidase inhibitors), the search for novel effective compounds is urgently needed. We evaluated a low molecular mass compound, enisamium iodide (FAV00A), against influenza virus infections in primary differentiated normal human bronc...
journal_title:Acta virologica
pub_type: 杂志文章
doi:10.4149/av_2018_211
更新日期:2018-01-01 00:00:00
abstract::Haemagglutinin (HA) and fusion (F) proteins of peste-des-petits-ruminants virus (PPRV) and rinderpest virus (RPV) were purified by immunoaffinity chromatography. The purified proteins were characterized by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate (SDS-PAGE). Rabbit hyperimmune sera ...
journal_title:Acta virologica
pub_type: 杂志文章
doi:
更新日期:1998-11-01 00:00:00
abstract::This study comprised 100 persons with antibodies to hepatitis C virus (HCV), including 77 intravenous drug users (IVDUs). They were tested with serological HCV typing assays (Murex HCV serotyping 1-6 assay; Chiron RIBA HCV Serotyping SIA). Patients with a positive polymerase chain reaction (PCR) for HCV (n = 66) were ...
journal_title:Acta virologica
pub_type: 杂志文章
doi:
更新日期:1997-10-01 00:00:00
abstract::Nucleoprotein (NP) complexes constituting the material of the postmicrosomal sediment (POMS) and its three basic components (A, B, C) (Ríman and Sulová, 1997a), harboring an extrachromosomal DNA closely related to AMV DNA were found to possess DNA- and RNA-synthesizing activities (SAs) reflecting the ability of this m...
journal_title:Acta virologica
pub_type: 杂志文章
doi:
更新日期:1997-08-01 00:00:00
abstract::The effect of Ribavirin treatment for the chemotherapy of several grapevine viruses was evaluated. Four grapevine cultivars were repeatedly treated with Ribavirin in two different concentrations and with three different lengths of treatment. Repeating the Ribavirin treatment always had a significant effect on the numb...
journal_title:Acta virologica
pub_type: 杂志文章
doi:10.4149/av_2016_04_400
更新日期:2016-01-01 00:00:00
abstract::The seed transmission of the Zucchini yellow mosaic virus (ZYMV) was studied in cucumber using two isolates unrelated in their biological characteristics. Although the virus could be readily detected in mature seeds harvested from infected cucumbers, the seedlings obtained from infected germinated seeds tested negativ...
journal_title:Acta virologica
pub_type: 杂志文章
doi:
更新日期:2007-01-01 00:00:00
abstract::Recently, mitochondria have been shown to have a vital role in the innate immune response to viral infection. In response, viruses such as adenovirus, have developed mechanisms to alter mitochondrial function through direct or indirect interaction with the mitochondria. The interaction of human recombinant adenoviral ...
journal_title:Acta virologica
pub_type: 杂志文章
doi:10.4149/av_2019_114
更新日期:2019-01-01 00:00:00
abstract::Histological changes were studied in the central nervous system (CNS) of 58 Macaca rhesus monkeys, infected intracerebrally (i.c.) and subcutaneously (s.c.) with tick-borne encephalitis (TBE) virus. Subacute degenerative process in the CNS occurred in 7 monkeys on days 15-24 post-infection (p.i.), while chronic degene...
journal_title:Acta virologica
pub_type: 杂志文章
doi:
更新日期:1984-05-01 00:00:00
abstract::The binding of immune and preimmune rabbit sera to fixed VERO cells infected with herpes simplex virus type 1 (HSV 1) was measured by quantitative immunofluorescence. False positive binding of the preimmune serum (PS) occurred from 8 hr post infection (p.i.), while the immune serum (IS) revealed positive fluorescence ...
journal_title:Acta virologica
pub_type: 杂志文章
doi:
更新日期:1979-09-01 00:00:00
abstract::In postnatally acquired lymphocytic choriomeningitis (LCM), specific immunofluorescent (IF) IgM antibody was found nearly in all the examined sera from 23 patients for 6-8 months after onset of the disease, IgA antibody in two-thirds of the patients and only during the first 5 weeks of the disease. In the first weeks,...
journal_title:Acta virologica
pub_type: 杂志文章
doi:
更新日期:1978-05-01 00:00:00
abstract::The effect of some cellular function inhibitors on adsorption and successive events of penetration of vesicular stomatitis virus to phylogenetically unrelated permissive cells was investigated. Treatment of HeLa, CER, EPC and Aedes albopictus cells with colcemide and cytochalasin D which affect cytoskeleton organizati...
journal_title:Acta virologica
pub_type: 杂志文章
doi:
更新日期:1988-11-01 00:00:00
abstract::Monoparacoumarylputrescinium chloride (pCPH), supplied to detached leaves of Gomphrena globosa via the petiole, induced interference with tomato bushy stunt virus infection by reducing the number and size of the necrotic local lesions. The phenolic compound neither inactivated directly the virus in vitro, nor induced ...
journal_title:Acta virologica
pub_type: 杂志文章
doi:
更新日期:1981-03-01 00:00:00
abstract::We followed the viral kinetics and histopathological changes in different organs of immunocompetent mice infected orally with coxsackieviruses B3 (CVB3) Nancy strain and B4 (CVB4) JVB strain separately. The viruses used were not adapted to mouse organs. In the acute phase of infection, the viral kinetics indicated vir...
journal_title:Acta virologica
pub_type: 杂志文章
doi:
更新日期:2003-01-01 00:00:00
abstract::Experimental parenteral inoculation of Dermacentor reticulatus ticks with the rickettsia Coxiella burnetii (C.b.) and tick-borne encephalitis (TBE) virus resulted in a generalized rickettsial and viral infection, irrespective of whether the agents were given simultaneously or by a 7 days interval apart. Both agents mu...
journal_title:Acta virologica
pub_type: 杂志文章
doi:
更新日期:1987-01-01 00:00:00
abstract::In result of 20 pregnancies complicated by varicella 11 healthy and 6 defective offspring were born, 2 pregnancies were aborted by the physician and one pregnancy terminated by stillbirth of 3 siblings. Laboratory investigation of 16 pregnancies has shown that transplacental transfer of varicella-zoster (VZ) virus did...
journal_title:Acta virologica
pub_type: 杂志文章
doi:
更新日期:1986-05-01 00:00:00
abstract::A rickettsia-like organism (RLO) was detected in the oocytes of Haemaphysalis inermis ticks. The RLOs were about 5 microns long bent rods with a definite inner structure. They were Gram-negative and could be visualized by Giemsa but not by Gimenez staining. Attempts to cultivate the RLO in chick embryo yolk sacs, vari...
journal_title:Acta virologica
pub_type: 杂志文章
doi:
更新日期:1981-09-01 00:00:00
abstract::Gamma irradiation is a non-thermal processing technique used to disinfect harmful microorganisms in agriculture. This technology has been shown to be an effective method to control bacterial and fungal plant pathogens. However, its effect on viral plant pathogen is less understood. Gamma irradiation was evaluated for ...
journal_title:Acta virologica
pub_type: 杂志文章
doi:10.4149/av_2017_02_14
更新日期:2017-01-01 00:00:00
abstract::Five H5N2 avian influenza viruses (AIVs) were isolated in Xianghai National Nature Reserve, Jilin Province, China in October 2011. They were all identified as low pathogenic AIVs (LPAIVs) based on their deduced amino acid sequences at the cleavage site of HA protein. Phylogenetic analysis revealed that all gene segmen...
journal_title:Acta virologica
pub_type: 杂志文章
doi:10.4149/av_2015_02_140
更新日期:2015-06-01 00:00:00
abstract::Human influenza virus A/Krasnodar/101/59 (H2N2) was passaged in chick fibroblast cultures in the presence of trypsin at suboptimal temperature. The virus which underwent 16 passages at 28 degrees C possessed cold-adapted (ca) and temperature sensitive (ts) phenotypes and formed larger plaques at the optimal temperatur...
journal_title:Acta virologica
pub_type: 杂志文章
doi:
更新日期:1984-09-01 00:00:00
abstract::The first confirmed evidence of scrapie in Slovakia was demonstrated in one sheep of the autochthonous Merino breed from the southeastern part of the country. The reported scrapie was diagnosed during compulsory transmissible spongiform encephalitis (TSE) screening of sheep over 9 months of age assigned for consumptio...
journal_title:Acta virologica
pub_type: 杂志文章
doi:
更新日期:2003-01-01 00:00:00
abstract:KEYWORDS:virus sanitation; in vitro explants; antiviral mechanism; virus longevity. ...
journal_title:Acta virologica
pub_type: 杂志文章
doi:10.4149/av_2012_02_159
更新日期:2012-01-01 00:00:00
abstract::Peripheral blood cells of avian myeloblastosis virus (AMV-(infected chickens were examined at various intervals post infection by immunofluorescence. AMV revertase was identified in pro- and myeloblasts; it was localized mainly in the perinuclear zone or throughout the cytoplasm. No revertase was found in erythrocytes...
journal_title:Acta virologica
pub_type: 杂志文章
doi:
更新日期:1976-08-01 00:00:00