Abstract：
:Mouse polyclonal antibodies were prepared against broad bean stain virus (BBSV, Comovirus group) and its coat protein subunits, large (L) and small (S) protein. These subunits were less immunogenic than native virus. Antibodies against L protein (Anti-L) and native virus (Anti-BBSV) did not react in immunoblots with S protein, but Anti-BBSV antibody reacted with S protein in plate-trapped antigen ELISA. Anti-S antibodies did not react with the related red clover mottle comovirus (RCMV), but Anti-L and Anti-BBSV antibodies reacted with RCMV similarly to BBSV. We assume that all the linear epitopes of the BBSV S protein are hidden in the native virions and the antigenic similarity between BBSV and RCMV is based mainly on their common linear L-specific epitopes.
journal_name
Acta Viroljournal_title
Acta virologicaauthors
Subr Z,Matisová J,Gallo Jsubject
Has Abstractpub_date
1994-06-01 00:00:00pages
129-32issue
3eissn
0001-723Xissn
1336-2305journal_volume
38pub_type
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