In situ detection of DNA-binding proteins in herpes simplex virus type 1-infected cells.

Abstract:

:An in situ assay for detecting DNA-binding proteins in herpes simplex virus type 1 (HSV-1)-infected cells is described. Seventeen HSV-induced DNA-binding species were visible with nicked, double-stranded DNA as a substrate, while fourteen virus-induced DNA-binding fractions were present in gels containing nuclease-treated, single-stranded DNA. The effects of HSV on cellular DNA-binding protein expression could also be seen. The resolution of DNA-binding fractions was dependent upon the type of DNA substrate utilized, high salt extraction of DNA-binding components and their physical separation from infected cell DNAs, dialysis of the high salt and the length of DNase treatment of gels following electrophoresis.

journal_name

Acta Virol

journal_title

Acta virologica

authors

Mohammad F,Farber E

subject

Has Abstract

pub_date

1989-09-01 00:00:00

pages

401-9

issue

5

eissn

0001-723X

issn

1336-2305

journal_volume

33

pub_type

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