Abstract：
:The RNA-dependent RNA polymerase (RdRp) in viruses of the family Flaviviridae plays an important role in the viral replication process and in the forming of a replicase complex. We used small interfering RNAs (siRNAs) corresponding to the highly conservative Motif V of RdRp gene of different viruses to examine their role in modulating the expression of RdRp. Evaluation of the expression of RdRps was performed by the fluorescence, flow cytometry, Western blotting, and real-time PCR. We found that Classical swine fever virus (CSFV) siRNA could completely block the transcription and expression of RdRp. Additionally, Hepatitis C virus (HCV) siRNA could cause effective inhibition of RdRp, whereas Japanese encephalitis virus siRNA did not show significant repression of corresponding RdRp. These results demonstrated that siRNAs inhibited the expression of tested RdRps at the transcription level or at the posttranscriptional processing to a different extent.
journal_name
Acta Viroljournal_title
Acta virologicaauthors
Xu Z,Li X,Liu R,Si Y,Sun M,Jin M,Chen H,Qian Psubject
Has Abstractpub_date
2007-01-01 00:00:00pages
195-201issue
3eissn
0001-723Xissn
1336-2305journal_volume
51pub_type
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