Abstract：
:The binding of immune and preimmune rabbit sera to fixed VERO cells infected with herpes simplex virus type 1 (HSV 1) was measured by quantitative immunofluorescence. False positive binding of the preimmune serum (PS) occurred from 8 hr post infection (p.i.), while the immune serum (IS) revealed positive fluorescence starting with 4 hr p.i. The false binding of PS decreased significantly (P = 0.01) if the cells were cultured in the presence of 2-deoxy-D-glucose (DOG). Statistical analysis of data on the binding of IS to infected cells kept in the presence or absence of DOG showed that the mean values of immunofluorescence were not significantly different (P less than 0.1). Cells cultured for 8 hr p.i. in the presence of 6 mM DOG seem to be a suitable antigen for detection of the actual level of specific antibodies by indirect immunofluorescence.
journal_name
Acta Viroljournal_title
Acta virologicaauthors
Rajcáni J,Hulíková Z,Mucha Vsubject
Has Abstractpub_date
1979-09-01 00:00:00pages
403-11issue
5eissn
0001-723Xissn
1336-2305journal_volume
23pub_type
杂志文章相关文献
ACTA VIROLOGICA文献大全abstract：:ORF 29 of Bombyx mori nucleopolyhedrovirus (BmNPV ORF29), a conserved gene among all the lepidopteran baculoviruses, was subjected to analysis of sequence and transcription/translation in the insect BmN cells in vitro. His-tagged BmNPV ORF29 fusion protein was expressed in Escherichia coli, purified and used for the p...
journal_title：Acta virologica
pub_type： 杂志文章
doi：10.4149/av_2010_04_275
更新日期：2010-01-01 00:00:00
abstract：:Red clover mottle virus was kept in 0.1 M phosphate buffer, pH 7.0, at 4, --20 and --60 degrees C for 1 and 4 weeks and 3 months. The degree of virion disintegration was the highest at --20 degrees C and the lowest at 4 degrees C. Two cycles of freezing and thawing destroyed the virions. In frozen samples (at both --2...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1977-01-01 00:00:00
abstract：:In vivo phagocytosis activity of macrophages (PAM) was temporarily suppressed in mice by application of a suspension of microscopic from particles. As demonstrated, a reversible block of 70% of PAM was accompanied by a marked increase of the lethality during the acute tick-borne encephalitis (TBE) virus infection. Asy...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1985-05-01 00:00:00
abstract：:The rate of the synthesis of tick-borne encephalitis (TBE) virus-specified proteins at high multiplicity of infection (MOI of 100 PFU per cell) was the highest by 8-14 hr post-infection (p.i.) as compared to the lower MOI of 4 PFU per cell (14 to 17 hr p.i.). The first virus-specific proteins appeared in cells from 2 ...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1986-07-01 00:00:00
abstract：:Replication of vaccinia virus DNA in chick embryo fibroblasts at a high multiplicity of infection started after 4-6 hr, reached a maximum at the 12th hr and was completed 18 hr after inoculation (p.i.). An analysis of newly synthesised DNAs in the period of the highest rate of synthesis revealed the formation of short...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1981-01-01 00:00:00
abstract：:Neonatal rat heart muscle primary cell cultures were infected with influenza A viruses. Depending on the infectious dose, functional and structural changes of the heart muscle cells could be registered. The system seems suitable for testing the cytotoxicity of different influenza virus strains. ...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1985-12-01 00:00:00
abstract：:Influenza is an ancient disease that has infected humans in irregular intervals throughout recorded history. The most infamous pandemic was "Spanish Flu" which affected large parts of the world population and killed in 1918-1919, at a rough estimate, at least 50 million people. More recently, two influenza A pandemics...
journal_title：Acta virologica
pub_type： 杂志文章,评审
doi：
更新日期：2006-01-01 00:00:00
abstract：:Eperythrozoon was detected in suckling mice inoculated with a suspension of nymphal Ixodes ricinus ticks. The organism was filterable through 220 nm Millipore membranes, moderately sensitive to diethyl ether, pathogenic to suckling but not to adult mice when given intracerebrally, intraperitoneally or subcutaneously, ...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1993-12-01 00:00:00
abstract：:A mathematical model of the pathogenesis of experimental Mengo virus infection in mice has been developed and fitted using kinetic data of both virus multiplication in different organs and mortality. The behaviour of the model proved to be bistable. In contrast to the widely accepted hypothesis that an acutely virus-i...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1987-08-01 00:00:00
abstract：:The techniques of reverse transcription-polymerase chain reaction (RT-PCR) and restriction analysis were used to differentiate highly virulent Indian field isolates of infectious bursal disease virus (IBDV) from vaccine strains. Primers were designed to amplify the variable region of VP2 gene coding for major virus ne...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1999-08-01 00:00:00
abstract：:Plum pox virus (PPV) isolates are grouped into three clusters differentiated by biological, serological, molecular and epidemiological characteristics: Marcus (M), Dideron (D) and Cherry (C). The El Amar (EA) isolate that does not fit any of the above groups is also known. Monoclonal antibodies (MAbs) that specificall...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1998-09-01 00:00:00
abstract：:In result of 20 pregnancies complicated by varicella 11 healthy and 6 defective offspring were born, 2 pregnancies were aborted by the physician and one pregnancy terminated by stillbirth of 3 siblings. Laboratory investigation of 16 pregnancies has shown that transplacental transfer of varicella-zoster (VZ) virus did...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1986-05-01 00:00:00
abstract：:Neopterin and soluble CD14 (sCD14) are detected at high levels in hepatitis C virus (HCV) infections. We aimed to evaluate the role of these plasma immune activation biomarkers, for the indirect assessment of immune activation status of patients with low anti-HCV reactivity and a HCV infection. Low anti-HCV reactivity...
journal_title：Acta virologica
pub_type： 杂志文章,多中心研究
doi：10.4149/av_2017_304
更新日期：2017-01-01 00:00:00
abstract：UNLABELLED:DNA vaccines containing the capsid precursor polypeptide P1 gene of foot-and-mouth disease virus (FMDV) alone or combined with the VP22 gene of bovine herpesvirus 1 (BVP22) as molecular adjuvant were constructed and used for immunization of BALB/c mice. The latter were challenged with FMDV and their humoral ...
journal_title：Acta virologica
pub_type： 杂志文章
doi：10.4149/av_2012_02_111
更新日期：2012-01-01 00:00:00
abstract：:The seed transmission of the Zucchini yellow mosaic virus (ZYMV) was studied in cucumber using two isolates unrelated in their biological characteristics. Although the virus could be readily detected in mature seeds harvested from infected cucumbers, the seedlings obtained from infected germinated seeds tested negativ...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：2007-01-01 00:00:00
abstract：:The achievements and perspectives of genetic manipulations are described aiming at preparation of first generation subunit vaccines based on the synthesis in bacterial and eukaryotic cells of full-sized virion proteins expressing the main antigenic determinants. The preparation of such vaccines in bacterial cells seem...
journal_title：Acta virologica
pub_type： 杂志文章,评审
doi：
更新日期：1985-05-01 00:00:00
abstract：:Antigenic relationships between the four subgroups of the Kemerovo serogroup of tick-borne orbiviruses were examined. Kemerovo (KEM subgroup), Broadhaven (BRD) and Wexford (WEX) [Great Island (GI) sub-group], Chenuda (CNU sub-group), and Wad Medani (WM sub-group) viruses cross-reacted in immunofluorescence tests. Comp...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1989-01-01 00:00:00
abstract：:A lipophilic thermostable lipopolysaccharide (LPS) complex was isolated by phenol extraction from purified suspensions of the typhus group rickettsiae. The LPS complex is antigenic and possesses some endotoxic properties such as toxicity for actinomycin D-treated mice, pyrogenicity for rabbits and guinea pigs, ability...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1977-09-01 00:00:00
abstract：:Monoclonal antibodies (MAbs) were prepared against ICP4 of Marek's disease virus (MDV). Mice were inoculated with ICP4 obtained from High-Five insect cells infected with a recombinant baculovirus expressing ICP4. MAbs were selected by enzyme-linked immunosorbent assay (ELISA) using MDV-infected and control chick kidne...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1999-04-01 00:00:00
abstract：:Avian infectious bronchitis virus (IBV) and avian pathogenic Escherichia coli (APEC) are two important respiratory pathogens in the chicken. The co-infection can lead to chronic complications and considerable economic losses in the poultry industry worldwide. In the present study, we compared differential transcriptio...
journal_title：Acta virologica
pub_type： 杂志文章
doi：10.4149/av_2020_413
更新日期：2020-01-01 00:00:00
abstract：:We isolated plaque-size variants of a South American strain of yellow fever virus, and compared their ability to infect orally and be transmitted by vector Aedes aegypti mosquitoes with that of the uncloned, parental virus. We analyzed the same clonal isolates in mouse virulence experiments. No significant differences...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1988-05-01 00:00:00
abstract：:Herpes simplex virus (HSV) DNA was found by spot blot hybridization in the right nc. amygdalae of 3 out 10 patients who underwent curative stereotactic surgery for severe mental retardation with aggressive behavior and/or paranoic schizophrenia. Of these, 6 were also tested for the presence of CMV DNA sequences with n...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1988-09-01 00:00:00
abstract：:Genetic composition and biological properties of influenza virus recombinants A/PR/8/34 (H1N1) and A/Greifswald/6/74 (H3N2) were analysed. The haemagglutinin (HA) gene of the strain A/PR/8/34 was shown an important part of the gene complex determining the virulence for mice, the yield of HA and the plaque forming capa...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1983-11-01 00:00:00
abstract：:Histology of skin lesions and demonstration in them of Rickettsia conorii by direct immunofluorescence test (DIF) are presented in 13 patients with Mediterranean spotted fever (MSF). The lymphohistiocytic vasculitis which dominated the picture is not specific, however, it could be suggestive for the diagnosis of ricke...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1991-11-01 00:00:00
abstract：:We attempted to quantify the protective potential of polyclonal IgG antibodies specific to the ectodomain of M2 protein (eM2) of influenza A virus (IAV) against lethal influenza infection of mice. For this purpose, eM2 conjugated with keyhole limpet hemocyanin (KLH) or KLH alone were administered with Freund's adjuvan...
journal_title：Acta virologica
pub_type： 杂志文章
doi：10.4149/av_2011_03_261
更新日期：2011-01-01 00:00:00
abstract：:Oral immunization of volunteers with a live tissue culture influenza A (H3N2) vaccine induced an increase in virus neutralizing (VN), haemagglutination inhibiting (HI) and neuraminidase inhibiting (NI) antibody. The dynamics of antibody and IgM and IgG immunoglobulin formation in the blood depended to a great extent o...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1979-01-01 00:00:00
abstract：:Induction of porcine interferon (PoIFN) with different lectins (phytohaemagglutinin, Lens culinaris lectin, Arachis hypogea lectin) in various concentrations (500, 50 and 5 micrograms/ml) was studied. Kinetics of PoIFN appearance was analyzed by antiviral and antiproliferative tests in human transformed and nontransfo...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1994-04-01 00:00:00
abstract：:In order to determine the definitive importance of T- and B-lymphocytes in immunity to Rickettsia conorii, mice genetically deficient in T-cells, B-cells, or both T- and B-cells were infected experimentally. T-lymphocytes rather than humoral antibodies were crucial to rickettsial clearance and a reduced mortality rate...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1984-11-01 00:00:00
abstract：:Chromosomal DNAs isolated from eight individuals from the Slovak population and from lymphoblastoid Namalwa cells were analyzed for the presence of genes coding for three subvariants of human interferon-alpha 2 (IFN-alpha 2), namely a, b, and c. The respective genes are regarded allelic, because they differ in the cod...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1994-04-01 00:00:00
abstract：:Chronic infection of HeLa cells was induced by an attenuated variant of Japanese encephalitis (JE) virus (HeLa-K3 cell line). The chronic infection was characterized by alternating phases of degeneration and recovery of the cell monolayer. JE virus was regularly released into the medium of chronically infected cell cu...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1980-12-01 00:00:00
