Abstract：
:ORF 29 of Bombyx mori nucleopolyhedrovirus (BmNPV ORF29), a conserved gene among all the lepidopteran baculoviruses, was subjected to analysis of sequence and transcription/translation in the insect BmN cells in vitro. His-tagged BmNPV ORF29 fusion protein was expressed in Escherichia coli, purified and used for the production of an antiserum. All the homologs of BmNPV ORF29 in baculoviruses contain a Nudix motif with slight modifications in the N-terminal part. A 654 nts-long transcript of BmNPV ORF29 in BmN cells was detected at 3-48 hrs p.i. indicating the early nature of the gene. In the virus-infected cells, the BmNPV ORF29 protein of 26 K was present in both the cytoplasm and nucleus. In the virions, the protein was present in Budded virus (BV), but not in Occlusion-derived virus (ODV). These results suggest that BmNPV ORF29 is a functional ORF of BmNPV, which encodes a 26 K protein expressed in the early stage of infection cycle.
journal_name
Acta Viroljournal_title
Acta virologicaauthors
Chen H,Li G,Huang G,Chen K,Yao Q,Guo Zdoi
10.4149/av_2010_04_275subject
Has Abstractpub_date
2010-01-01 00:00:00pages
275-80issue
4eissn
0001-723Xissn
1336-2305journal_volume
54pub_type
杂志文章相关文献
ACTA VIROLOGICA文献大全abstract：:A recently developed helper virus-free HSV-1 vector system has potential for both gene therapy and physiological studies, but relatively low vector titers have complicated use of this system. In this report, we explored improving the vector titers by isolating a Vero cell line that uses the tetracycline-regulated prom...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：2000-12-01 00:00:00
abstract：:The cold-adapted temperature-sensitive (ts) influenza virus strain A/Leningrad/134/17/57 (H2N2) multiplied well at 32 degrees C (optimal temperature); lower titres of infectious virus were obtained in developing chick embryos at 40 degrees C. In a canine kidney (MDCK) cell line and in primary calf kidney (CK) cells an...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1982-07-01 00:00:00
abstract：:The rate of the synthesis of tick-borne encephalitis (TBE) virus-specified proteins at high multiplicity of infection (MOI of 100 PFU per cell) was the highest by 8-14 hr post-infection (p.i.) as compared to the lower MOI of 4 PFU per cell (14 to 17 hr p.i.). The first virus-specific proteins appeared in cells from 2 ...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1986-07-01 00:00:00
abstract：:Evidence has been obtained indicating the association of certain biological properties of Rickettsia prowazekii with their capacity for persistence. Highly virulent rickettsial cultures inducing increased levels of complement-fixing antibodies were shown to cause a high percentage of rickettsial carriers in infected c...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1983-11-01 00:00:00
abstract：:Transfer factor specific for herpes simplex virus (HSV) type 1 (TFHSV-1) was prepared from splenic cells of HSV-1 immunized mice. Protection was transferred with TFHSV-1 to nonimmune mouse recipients. The TFHSV-1 injected mice had a higher survival rate after lethal HSV-1 challenge as compared to mice injected with a ...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1987-11-01 00:00:00
abstract：:Numerous cytokines and chemokines are involved in inflammatory and immune response. Whereas some of them inhibit virus replication in vitro directly or increase the patients' T4-lymphocyte level, others effects are not so clear. Using human immunodeficiency virus (HIV) and cell cultures we have studied the antiviral e...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1999-02-01 00:00:00
abstract：:The equine influenza virus, Ludhiana/5/87, isolated from the clinical material during the epidemic of equine influenza in India in 1987 was inhibited in haemagglutination-inhibition test by the antiserum against the prototype A/eq/Prague/1/56 (H7N7) virus and by post-epidemic horse sera. In haemagglutinin and neuramin...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1994-02-01 00:00:00
abstract：:Some differences were established between mopyridone-sensitive (MCU-s) wild strain and mopyridone-resistant (MCU-r) mutant progenies of influenza virus A/Hong Kong/1/68 (H3N2). The virions of MCU-r mutant had a lower buoyant density in linear sucrose gradient as compared to those of MCU-s strain, and an increased abil...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1995-04-01 00:00:00
abstract：:In the summer 1999, a measles outbreak occurred in Uruguai. During this outbreak 58 cases were recorded, 36 of which were laboratory confirmed as positive for measles virus (MV) IgM. The cases occurred in touristic places (Montevideo and Maldonado) predominantly among health facilities and tourist service personnel. U...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：2000-02-01 00:00:00
abstract：:Tick-borne viruses are causative agents of several important human diseases. Tick-borne encephalitis virus (TBEV) is the most prominent representative considered medically to be the most important arbovirus (arthropod-borne virus) in Europe and northern Asia. Tick-borne virus transmission cycles are determined by the ...
journal_title：Acta virologica
pub_type： 杂志文章,评审
doi：10.4149/av_2013_02_123
更新日期：2013-01-01 00:00:00
abstract：:Identification of structural protein relationships is likely to be important for virus assembly and anti-WSSV strategies. In this paper, VP90 of white spot syndrome virus (WSSV) was characterized. Temporal-transcription analysis showed that VP90 is expressed in the late stage of WSSV infection. Far-western and matrix-...
journal_title：Acta virologica
pub_type： 杂志文章
doi：10.4149/av_2012_01_57
更新日期：2012-01-01 00:00:00
abstract：:The uses of the nonionic detergent Triton X-100 (TX-100) and the cationic cetyltrimethylammonium bromide (CTAB) for the production of tick-borne encephalitis (TBE) virus subunits were compared. TX-100 splitted the virus into haemagglutinating envelope components which contain the virion proteins V3 and V1 and into a l...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1979-05-01 00:00:00
abstract：:A new cell line from the embryonic tissue of Culex tritaeniorhynchus mosquito was established. Morphological studies carried out at the 45th passage level (P-45) showed four different cell types viz. epithelial-like cells, fibroblast-like cells, giant cells and vacuolated cells. Karyological studies indicated diploid ...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：2002-01-01 00:00:00
abstract：:Chikungunya (CHIK) virus is prevalent throughout Southeast Asia and Africa. It has caused numerous large outbreaks in India. No active or passive surveillance has been carried out since the last epidemic occurring in 1971. During a recent outbreak of Dengue (DEN)-like illness in eastern India, Aedes aegypti mosquitoes...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：2001-01-01 00:00:00
abstract：:The cytotoxicity and antiviral activity of the caffeic acid oxidation product (KOP), a higher molecular polyphenolic compound of strong antiviral activity against herpesvirus hominis type 1 and type 2 (HVH 1, HVH 2), were tested in 6 cell cultures: rabbit kidney primary cells (RKP), rabbit testis primary cells (RTP), ...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1983-05-01 00:00:00
abstract：:Sensitivity and specificity of a modified agar gel immuno-diffusion (AGID) test for the diagnosis of enzootic bovine leukosis was compared in 609 sera using the standard method, the modified test, and ELISA. The modifications concerned the composition of agar gel (1.8% Bacto-agar Difco), addition of polyethylene glyco...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1989-03-01 00:00:00
abstract：:An assay protocol based on exploiting the polymerase chain reaction (PCR) for the direct detection of influenza B virus in throat swabs is described. By the use of PCR with nested primers, it was possible to detect the virus in throat swabs. Dilution experiments showed that as little as 1 plaque forming unit of virus ...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1992-05-01 00:00:00
abstract：:In order to investigate the growth of hepatitis A virus (HAV) in murine cells, L929 cells of the established mouse cell line were transfected with the virion RNA or infected with the virions and examined for the formation of negative-strand RNA and the rise of the viral infectivity titer. In both the transfected and i...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1996-09-01 00:00:00
abstract：:A nation-wide vaccination against mumps that had been launched in the Czech Republic in 1987 eliminated great outbreaks (up to 100,000 cases per year) of this disease in 1955-1988, but did not prevent small outbreaks (a few thousand cases per year) in 1995-1996, 2005-2007, and 2010-2012. The extent of these small outb...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：2013-01-01 00:00:00
abstract：:The binding of immune and preimmune rabbit sera to fixed VERO cells infected with herpes simplex virus type 1 (HSV 1) was measured by quantitative immunofluorescence. False positive binding of the preimmune serum (PS) occurred from 8 hr post infection (p.i.), while the immune serum (IS) revealed positive fluorescence ...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1979-09-01 00:00:00
abstract：:Mouse polyclonal antibodies were prepared against broad bean stain virus (BBSV, Comovirus group) and its coat protein subunits, large (L) and small (S) protein. These subunits were less immunogenic than native virus. Antibodies against L protein (Anti-L) and native virus (Anti-BBSV) did not react in immunoblots with S...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1994-06-01 00:00:00
abstract：:Among diseases transmitted by ticks in the Czech Republic, tick-borne encephalitis (TBE) caused by Tick-borne encephalitis virus (TBEV) and Lyme disease caused by Borrelia burgdorferi spirochete are most important. We propose an effective and specific test for detection of TBEV in a single tick or a pool of ticks base...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：2004-01-01 00:00:00
abstract：:Mouse sera collected from day 4 to day 133 postinfection (p.i.) with phase I Coxiella burnetii strain Nine Mile were analysed by immunoblotting with phase I C. burnetii cell lysate. Antibodies of IgG class protein antigens were revealed already on day 10 p.i. (60, 49 and 27 kD proteins), followed by those to 77 kD pro...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1992-01-01 00:00:00
abstract：:Herpes zoster (HZ) is a neurocutaneous disease caused by Varicella-zoster virus (VZV) as a consequence of declined cell-mediated immunity, immune suppression and immunodeficiency. As reactivation of JC polyomavirus (JCPyV) might be linked with immunodeficiency or immunosuppressive therapy, the relationship between HZ ...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：2006-01-01 00:00:00
abstract：:Thirty four chemical compounds were injected into rabies infected mice by intramuscular (i.m.) route. Twenty four compounds such as well known therapeutic agents: amantadine, lipacids, phenol compounds, didemnin-B, procaine, nucleosides analogues (ribavirin, tiazofurin, pyrazofurin) had no effect. Two compounds had a ...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1988-01-01 00:00:00
abstract：:Acute gastroenteritis (AGE) is one of the most frequently occuring illnesses in children and adults worldwide. In February 2017, two AGE outbreaks occurred in two adjacent schools in Huzhou city, Zhenjiang province of China. We detected high percentages of recombinant norovirus GII.P16/GII.2 in one school and chicken ...
journal_title：Acta virologica
pub_type： 杂志文章
doi：10.4149/av_2019_312
更新日期：2019-01-01 00:00:00
abstract：:Every year, the poultry industry experiences significant economic losses due to epidemics of Newcastle disease virus (NDV). Developing new vaccines by identifying and using the immunogenic hemagglutinin-neuraminidase (HN) protein can protect the poultry industry. In the present study, the full-length HN protein was ex...
journal_title：Acta virologica
pub_type： 杂志文章
doi：10.4149/av_2020_310
更新日期：2020-01-01 00:00:00
abstract：:An improved method of isolation of Coxiella burnetti proteins was developed. It consists of a combination of detergent (sodium dodecyl sulphate (SDS) or sodium deoxycholate (DOC) and hot phenol treatments. The resulting phenol phase (PP) contained either lipopolysaccharide-(LPS) free proteins (DOC extraction) or prote...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1996-02-01 00:00:00
abstract：:Peanut stripe virus (PStV) is one of the most common viruses infecting peanut that causes great economic losses every year. The 3ʹ-terminal 1082 bp of 74 PStV isolates collected from 12 districts of Shandong province, China were sequenced. Their coat protein (CP) genes were 864 bp in length and shared identities of 98...
journal_title：Acta virologica
pub_type： 杂志文章
doi：10.4149/av_2011_02_123
更新日期：2011-01-01 00:00:00
abstract：:Nonlethal infection was induced in laboratory mice with sublethal doses of rabies strains isolated from small wild rodents. Three types of nonlethal infecion were observed: persistent--with virus irregularly reisolable for at least 56 days after inoculation: latent--with negative virus reisolation but presence of infe...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1980-09-01 00:00:00
