Abstract:
:Every year, the poultry industry experiences significant economic losses due to epidemics of Newcastle disease virus (NDV). Developing new vaccines by identifying and using the immunogenic hemagglutinin-neuraminidase (HN) protein can protect the poultry industry. In the present study, the full-length HN protein was expressed in Escherichia coli (E. coli) BL21 (DE3) cells, purified via affinity chromatography and detected via western blot analysis using His-specific antibodies. The purified HN protein was further evaluated in chickens to study the immune response against NDV. The successful production of HN-specific IgY proved the activity of the purified HN protein. IgY was present in the serum of immunized chickens. However, the immune response was higher in chickens immunized with purified HN protein along with complete and incomplete adjuvants than in chickens immunized with only the HN protein. Keywords: protein; Newcastle disease virus; poultry; infectious diseases; vaccines.
journal_name
Acta Viroljournal_title
Acta virologicaauthors
Shahid N,Rao AQ,Ahad A,Gul A,Latif A,Azam S,Shahid M,Akhtar S,Shahid AA,Husnain Tdoi
10.4149/av_2020_310subject
Has Abstractpub_date
2020-01-01 00:00:00pages
331-337issue
3eissn
0001-723Xissn
1336-2305journal_volume
64pub_type
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