E. coli expression and immunological assessment of expressed recombinant Newcastle disease virus hemagglutinin-neuraminidase protein in chickens.

Abstract:

:Every year, the poultry industry experiences significant economic losses due to epidemics of Newcastle disease virus (NDV). Developing new vaccines by identifying and using the immunogenic hemagglutinin-neuraminidase (HN) protein can protect the poultry industry. In the present study, the full-length HN protein was expressed in Escherichia coli (E. coli) BL21 (DE3) cells, purified via affinity chromatography and detected via western blot analysis using His-specific antibodies. The purified HN protein was further evaluated in chickens to study the immune response against NDV. The successful production of HN-specific IgY proved the activity of the purified HN protein. IgY was present in the serum of immunized chickens. However, the immune response was higher in chickens immunized with purified HN protein along with complete and incomplete adjuvants than in chickens immunized with only the HN protein. Keywords: protein; Newcastle disease virus; poultry; infectious diseases; vaccines.

journal_name

Acta Virol

journal_title

Acta virologica

authors

Shahid N,Rao AQ,Ahad A,Gul A,Latif A,Azam S,Shahid M,Akhtar S,Shahid AA,Husnain T

doi

10.4149/av_2020_310

subject

Has Abstract

pub_date

2020-01-01 00:00:00

pages

331-337

issue

3

eissn

0001-723X

issn

1336-2305

journal_volume

64

pub_type

杂志文章
  • Rapid detection of respiratory syncytial virus in clinical specimens.

    abstract::Results of the direct immunofluorescence (IF) test in 152 clinical specimens (throat swabs) were compared with those of respiratory syncytial virus (RSV) isolation. The prevalence of RSV infections in the upper and lower respiratory tract was high especially in infants until 12 months of age. The average RSV isolation...

    journal_title:Acta virologica

    pub_type: 杂志文章

    doi:

    authors: Mlinarić-Galinović G,Ugrcić I,Cvetković M,Pende B,Ivanković D

    更新日期:1987-09-01 00:00:00

  • A new cell line from the embryonic tissues of Culex tritaeniorhynchus and its susceptibility to certain flaviviruses.

    abstract::A new cell line from the embryonic tissue of Culex tritaeniorhynchus mosquito was established. Morphological studies carried out at the 45th passage level (P-45) showed four different cell types viz. epithelial-like cells, fibroblast-like cells, giant cells and vacuolated cells. Karyological studies indicated diploid ...

    journal_title:Acta virologica

    pub_type: 杂志文章

    doi:

    authors: Athawale SS,Sudeep AB,Barde PV,Jadi R,Pant U,Mishra AC,Mourya DT

    更新日期:2002-01-01 00:00:00

  • Increased incidence of mumps in the Czech Republic in the years 2011 and 2012.

    abstract::A nation-wide vaccination against mumps that had been launched in the Czech Republic in 1987 eliminated great outbreaks (up to 100,000 cases per year) of this disease in 1955-1988, but did not prevent small outbreaks (a few thousand cases per year) in 1995-1996, 2005-2007, and 2010-2012. The extent of these small outb...

    journal_title:Acta virologica

    pub_type: 杂志文章

    doi:

    authors: Lexová P,Limberková R,Cástková J,Kynčl J

    更新日期:2013-01-01 00:00:00

  • JC polyomavirus reactivation is not associated with herpes zoster.

    abstract::Herpes zoster (HZ) is a neurocutaneous disease caused by Varicella-zoster virus (VZV) as a consequence of declined cell-mediated immunity, immune suppression and immunodeficiency. As reactivation of JC polyomavirus (JCPyV) might be linked with immunodeficiency or immunosuppressive therapy, the relationship between HZ ...

    journal_title:Acta virologica

    pub_type: 杂志文章

    doi:

    authors: Jeong BH,Park SJ,Koo DW,Kim YS

    更新日期:2006-01-01 00:00:00

  • Kinetic analysis of RNA editing of Newcastle disease virus P gene in the early period of infection.

    abstract:UNLABELLED:As a paramyxovirus, Newcastle disease virus (NDV) has the ability to edit its P (phosphoprotein) gene to synthesize three kinds of viral protein (P, V and W). It is technically very difficult to differentiate P, V and W mRNAs, and little was known about NDV regulation of RNA-editing frequency. To investigate...

    journal_title:Acta virologica

    pub_type: 杂志文章

    doi:10.4149/av_2016_01_71

    authors: Qiu X,Fu Q,Meng C,Yu S,Zhan Y,Dong L,Ren T,Sun Y,Tan L,Song C,Han X,Ding C

    更新日期:2016-03-01 00:00:00

  • Latent infection and measles meningoencephalitis in Syrian hamsters.

    abstract::A strain designated 42-Edmonston was isolated from the brain of a Syrian hamster 30 days after inoculation during the neonatal period with the reference "wild" measles virus strain Edmonston which produced a latent infection accompanied by production of measles antibody and insignificant pathomorphological changes. In...

    journal_title:Acta virologica

    pub_type: 杂志文章

    doi:

    authors: Eliseeva IYa,Ravkina LI,Karmysheva VYa

    更新日期:1977-05-01 00:00:00

  • Increased virulence of Marek's disease virus type 1 vaccine strain CV1988 after adaptation to qt35 cells.

    abstract::CVI988/Rispens strain of Marek's disease virus type I (MDV-1) is widely used as efficient vaccine to control Marek's disease (MD) in chicken flocks. Similarly to other live MD vaccine viruses it is propagated in freshly prepared chicken embryo fibroblasts (CEF). In this study, MDV-1 CVI988/Rispens strain was adapted t...

    journal_title:Acta virologica

    pub_type: 杂志文章

    doi:

    authors: Majerciak V,Valkova A,Szabová D,Geerligs H,Zelník V

    更新日期:2001-04-01 00:00:00

  • Effect of cellular inhibitors on the infection of various susceptible cells with vesicular stomatitis virus.

    abstract::The effect of some cellular function inhibitors on adsorption and successive events of penetration of vesicular stomatitis virus to phylogenetically unrelated permissive cells was investigated. Treatment of HeLa, CER, EPC and Aedes albopictus cells with colcemide and cytochalasin D which affect cytoskeleton organizati...

    journal_title:Acta virologica

    pub_type: 杂志文章

    doi:

    authors: Superti F,Seganti L,Orsi N

    更新日期:1988-11-01 00:00:00

  • Comparative trachea transcriptome analysis between avian infectious bronchitis virus and avian pathogenic E. coli individual infection and co-infection in SPF chickens.

    abstract::Avian infectious bronchitis virus (IBV) and avian pathogenic Escherichia coli (APEC) are two important respiratory pathogens in the chicken. The co-infection can lead to chronic complications and considerable economic losses in the poultry industry worldwide. In the present study, we compared differential transcriptio...

    journal_title:Acta virologica

    pub_type: 杂志文章

    doi:10.4149/av_2020_413

    authors: Hashemi S,Ghalyanchilangeroudi A,Hosseini SM,Sheikhi N

    更新日期:2020-01-01 00:00:00

  • Coxiella burnetii phase I and II proteins studied by SDS-page.

    abstract::Coxiella burnetii cells in both phase I and II reveal in sodium dodecylsulphate-polyacrylamide gel electrophoresis (SDS-PAGE) similar protein profiles with only small differences. C. burnetii protein profile in SDS-PAGE depended on the method of purification of C. burnetii cells from chick embryo yolk sacs. Immune mou...

    journal_title:Acta virologica

    pub_type: 杂志文章

    doi:

    authors: Lukácová M,Kazár J,Gajdosová E

    更新日期:1994-10-01 00:00:00

  • Influenza virus M2 protein and haemagglutinin conformation changes during intracellular transport.

    abstract::The influenza virus M2 protein has an ion channel activity that permits ions to enter the virion during its uncoating and also modulates pH of intracellular compartments. M2 protein is a homotetramer consisting of either a pair of disulfide-linked dimers or a disulfide-linked tetramer. The M2 trans-membrane domain pep...

    journal_title:Acta virologica

    pub_type: 杂志文章,评审

    doi:

    authors: Ciampor F,Cmarko D,Cmarková J,Závodská E

    更新日期:1995-06-01 00:00:00

  • Nature and antigen-specific activities of transfer factor against herpes simplex virus type 1.

    abstract::Transfer factor specific for herpes simplex virus (HSV) type 1 (TFHSV-1) was prepared from splenic cells of HSV-1 immunized mice. Protection was transferred with TFHSV-1 to nonimmune mouse recipients. The TFHSV-1 injected mice had a higher survival rate after lethal HSV-1 challenge as compared to mice injected with a ...

    journal_title:Acta virologica

    pub_type: 杂志文章

    doi:

    authors: Huang LL,Su CZ,Wan ZF

    更新日期:1987-11-01 00:00:00

  • Histopathology and immunopathology of skin biopsy specimens in Mediterranean spotted fever.

    abstract::Histology of skin lesions and demonstration in them of Rickettsia conorii by direct immunofluorescence test (DIF) are presented in 13 patients with Mediterranean spotted fever (MSF). The lymphohistiocytic vasculitis which dominated the picture is not specific, however, it could be suggestive for the diagnosis of ricke...

    journal_title:Acta virologica

    pub_type: 杂志文章

    doi:

    authors: Dujella J,Morović M,Dzelalija B,Gverić M,Novaković S

    更新日期:1991-11-01 00:00:00

  • Virion proteins and the perspectives of gene manipulations in vaccine preparation.

    abstract::The achievements and perspectives of genetic manipulations are described aiming at preparation of first generation subunit vaccines based on the synthesis in bacterial and eukaryotic cells of full-sized virion proteins expressing the main antigenic determinants. The preparation of such vaccines in bacterial cells seem...

    journal_title:Acta virologica

    pub_type: 杂志文章,评审

    doi:

    authors: Tickhonenko TI

    更新日期:1985-05-01 00:00:00

  • VP90 of white spot syndrome virus interacts with VP26 and VP28.

    abstract::Identification of structural protein relationships is likely to be important for virus assembly and anti-WSSV strategies. In this paper, VP90 of white spot syndrome virus (WSSV) was characterized. Temporal-transcription analysis showed that VP90 is expressed in the late stage of WSSV infection. Far-western and matrix-...

    journal_title:Acta virologica

    pub_type: 杂志文章

    doi:10.4149/av_2012_01_57

    authors: Li Q,Liu QH,Huang J

    更新日期:2012-01-01 00:00:00

  • Autoantibodies in sera of influenza patients.

    abstract::Sera of the influenza patients and healthy controls were tested for some types of autoantibody (SMA, ANA, ABBA, AMA). They were detected in 83.8% of the patients' sera and in 16.6% of controls. SMA were present in 77.4%, ANA in 54.8%, and ABBA in 16.1% of the patient's sera. AMA were not detected. A majority of the se...

    journal_title:Acta virologica

    pub_type: 杂志文章

    doi:

    authors: Loza-Tulimowska M,Semkow R,Michalak T,Nowoslawski A

    更新日期:1976-06-01 00:00:00

  • Detection by immunofluorescence of avian myeloblastosis virus reverse transcriptase.

    abstract::Peripheral blood cells of avian myeloblastosis virus (AMV-(infected chickens were examined at various intervals post infection by immunofluorescence. AMV revertase was identified in pro- and myeloblasts; it was localized mainly in the perinuclear zone or throughout the cytoplasm. No revertase was found in erythrocytes...

    journal_title:Acta virologica

    pub_type: 杂志文章

    doi:

    authors: Karmysheva VY,Graevskaya NA,Sito AF,Sushko LN

    更新日期:1976-08-01 00:00:00

  • Protective efficacy of IFN-ω AND IFN-λs against influenza viruses in induced A549 cells.

    abstract::The interferon system represents one of the components of the first line defence against influenza virus infection. Interferon omega (IFN-ω) is antigenetically different from IFN-α and IFN-β and can affect patients who are resistant to these IFNs. To improve the biological characterization of IFN-ω, we compared its ac...

    journal_title:Acta virologica

    pub_type: 杂志文章

    doi:10.4149/av_2015_04_413

    authors: Škorvanová L,Švančarová P,Svetlíková D,Betáková T

    更新日期:2015-12-01 00:00:00

  • Dynamic model of the pathogenesis of Mengo virus infection in mice.

    abstract::A mathematical model of the pathogenesis of experimental Mengo virus infection in mice has been developed and fitted using kinetic data of both virus multiplication in different organs and mortality. The behaviour of the model proved to be bistable. In contrast to the widely accepted hypothesis that an acutely virus-i...

    journal_title:Acta virologica

    pub_type: 杂志文章

    doi:

    authors: Guthke R,Veckenstedt A,Güttner J,Stracke R,Bergter F

    更新日期:1987-08-01 00:00:00

  • Cell-mediated immune reactions in measles.

    abstract::Cell-mediated reactions in measles cases, in direct contacts and healthy children were tested. Indices of phytohaemagglutinin-stimulated blastic transformation and leukocyte migration inhibition were evaluated. Positive reactions were found in the first and second weeks after manifestation of clinical symptoms. The ap...

    journal_title:Acta virologica

    pub_type: 杂志文章

    doi:

    authors: Kańtoch M,Abramow-Newerly W,Jankowski M,Imbs D,Litwińska B,Stepień R,Slowińska I,Kubica J,Boguslawska J

    更新日期:1980-03-01 00:00:00

  • Production of defective virus by terminally differentiated myotubes infected with Rous sarcoma virus.

    abstract::The generally accepted concept that the replication of Rous sarcoma virus (RSV) is dependent on host cell DNA synthesis was reexamined. As the host we used terminally differentiated myotubes (MT), in which no cellular DNA synthesis is observed. As an extension of our previous study which indicated that RSV-infected MT...

    journal_title:Acta virologica

    pub_type: 杂志文章

    doi:

    authors: Park HT,Hara H,Fujihara M,Hsia KJ,Tanaka A,Furuse T,Iwasaki Y,Kaji A

    更新日期:1995-09-01 00:00:00

  • Tick-borne encephalitis virus-specific RT-PCR--a rapid test for detection of the pathogen without viral RNA purification.

    abstract::Among diseases transmitted by ticks in the Czech Republic, tick-borne encephalitis (TBE) caused by Tick-borne encephalitis virus (TBEV) and Lyme disease caused by Borrelia burgdorferi spirochete are most important. We propose an effective and specific test for detection of TBEV in a single tick or a pool of ticks base...

    journal_title:Acta virologica

    pub_type: 杂志文章

    doi:

    authors: Rudenko N,Golovchenko M,Cihlárova V,Grubhoffer L

    更新日期:2004-01-01 00:00:00

  • Oligonucleotide fingerprint analysis on Japanese encephalitis virus strains after passage histories.

    abstract::Genetic variation in Japanese encephalitis (JE) virus strains after various passage histories was examined by oligonucleotide fingerprints of the 42S genome RNA. The results indicated that genome changes occurred during passages of new virus isolates through cell lines or mouse brains, especially at earlier passage le...

    journal_title:Acta virologica

    pub_type: 杂志文章

    doi:

    authors: Hori H,Igarashi A,Yoshida I,Takagi M

    更新日期:1986-09-01 00:00:00

  • Next generation tau models in Alzheimer's disease research - virus based gene delivery systems.

    abstract::Alzheimer's disease (AD) the most common form of dementia is characterized by cognitive decline and progressive loss of neurons in the central nervous system. Despite huge scientific progress, there are only few animal models that recapitulate at least majority of the AD pathology and related symptomatology. Therefore...

    journal_title:Acta virologica

    pub_type: 杂志文章,评审

    doi:10.4149/av_2017_01_13

    authors: Cubinková V,Valachová B,Brezováková V,Szabó R,Zimová I,Kostecká Z,Jadhav S

    更新日期:2017-01-01 00:00:00

  • Loss of infectivity of red clover mottle virus by lysolecithin.

    abstract::2-0-hexadecyl-glycero-3-phosphocholine (GPC) 1 mmol/l solution reduced the infectivity of red clover mottle virus (RCMV) by over 90%. The binding of GPC molecules to RCMV particles was investigated with 1H-nmr spectroscopy, which showed a striking interaction between virus particles and GPC molecules. In 10 mmol/l GPC...

    journal_title:Acta virologica

    pub_type: 杂志文章

    doi:

    authors: Kluge S,Gawrisch K,Nuhn P

    更新日期:1987-03-01 00:00:00

  • Partial molecular characterization of a mild isolate of Grapevine fanleaf virus from South Moravia, Czech Republic.

    abstract::An atypical mild isolate HV5 of Grapevine fanleaf virus (GFLV) was found in a South Moravian viticulture region in Czech Republic. Partial sequence of its RNA2 was determined and compared with available sequences of typical GFLV isolates. Two genomic regions, namely a 814 nt-long one spanning the movement protein (MP)...

    journal_title:Acta virologica

    pub_type: 杂志文章

    doi:

    authors: Komínek P,Bryxiová M,Glasa M

    更新日期:2006-01-01 00:00:00

  • Molecular detection and characterization of phytoplasma in association with begomovirus in eggplant.

    abstract::The samples from eggplants showing mixed symptoms of little leaf and mosaic were collected from two districts (Mirzapur and Varanasi) of Uttar Pradesh, India. The total nucleic acid extracted from these samples was amplified by PCR using universal 16S rRNA primers specific to phytoplasma and primers specific to DNA-A-...

    journal_title:Acta virologica

    pub_type: 杂志文章

    doi:10.4149/av_2018_218

    authors: Venkataravanappa V,Prasanna HC,Lakshminarayana CN,Reddy MK

    更新日期:2018-01-01 00:00:00

  • To the antigenic classification of some viruses from the tick-borne encephalitis complex by monoclonal antibodies.

    abstract::Two monoclonal antibodies (MoAb) to the Skalica virus from the tick-borne encephalitis (TBE) complex were used to compare Karshi and Royal Farm viruses with the Russian spring-summer encephalitis, Central European encephalitis (Hypr and Kumlinge strains) Skalica, Langat and Powassan viruses. The first MoAb was prepare...

    journal_title:Acta virologica

    pub_type: 杂志文章

    doi:

    authors: Gresíková M,Sekeyová M

    更新日期:1990-02-01 00:00:00

  • Detection of antibodies to the western and the eastern type of haemorrhagic fever with renal syndrome in patient's sera from Slovakia.

    abstract::We present serological evidence of infection with western and eastern type of haemorrhagic fever with renal syndrome (HFRS) virus in patient's sera from Slovakia by indirect immunofluorescence. Treatment of sera with 2-mercaptoethanol decreased levels of haemagglutination-inhibition (HI) antibodies to the eastern type...

    journal_title:Acta virologica

    pub_type: 杂志文章

    doi:

    authors: Gresíková M,Tkachenko EA,Sekeyová M

    更新日期:1990-08-01 00:00:00

  • Ribonuclease-resistant RNA of Kemerovo virus.

    abstract::RNA of partially purified Kemerovo virus sedimented in a relatively broad region in sucrose density gradient with a peak at 14 S. Approximately 65% of this RNA was resistant to the action of ribonuclease which indicates the double-stranded nature of Kemerovo virus RNA. ...

    journal_title:Acta virologica

    pub_type: 杂志文章

    doi:

    authors: Rosenbergová M,Slávik I

    更新日期:1975-01-01 00:00:00