Abstract:
:Identification of structural protein relationships is likely to be important for virus assembly and anti-WSSV strategies. In this paper, VP90 of white spot syndrome virus (WSSV) was characterized. Temporal-transcription analysis showed that VP90 is expressed in the late stage of WSSV infection. Far-western and matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS) assay showed that VP90 interacts with VP26 and VP28, two major envelope proteins. Far-western blot with recombinant VP26 (rVP26), N-terminal end of VP28 (rVP28N) and C-terminal end of VP28 (rVP28C) provided further evidence for interaction of rVP90 and rVP26 or rVP28. These results suggest that VP90 is anchored to the envelope through interacting with VP26 and VP28.
journal_name
Acta Viroljournal_title
Acta virologicaauthors
Li Q,Liu QH,Huang Jdoi
10.4149/av_2012_01_57subject
Has Abstractpub_date
2012-01-01 00:00:00pages
57-62issue
1eissn
0001-723Xissn
1336-2305journal_volume
56pub_type
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