VP90 of white spot syndrome virus interacts with VP26 and VP28.

Abstract:

:Identification of structural protein relationships is likely to be important for virus assembly and anti-WSSV strategies. In this paper, VP90 of white spot syndrome virus (WSSV) was characterized. Temporal-transcription analysis showed that VP90 is expressed in the late stage of WSSV infection. Far-western and matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS) assay showed that VP90 interacts with VP26 and VP28, two major envelope proteins. Far-western blot with recombinant VP26 (rVP26), N-terminal end of VP28 (rVP28N) and C-terminal end of VP28 (rVP28C) provided further evidence for interaction of rVP90 and rVP26 or rVP28. These results suggest that VP90 is anchored to the envelope through interacting with VP26 and VP28.

journal_name

Acta Virol

journal_title

Acta virologica

authors

Li Q,Liu QH,Huang J

doi

10.4149/av_2012_01_57

subject

Has Abstract

pub_date

2012-01-01 00:00:00

pages

57-62

issue

1

eissn

0001-723X

issn

1336-2305

journal_volume

56

pub_type

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