Abstract：
:In this study, a major part of genome of the pestivirus isolate 297 from Slovakia, comprising the 7195 nt-long 5΄-UTR-NS3 region was sequenced and analyzed. Conserved cleavage sites between individual viral proteins of this region were determined and the number of amino acids of respective proteins was estimated as follows: 168 for Npro, 100 for C, 227 for Erns, 195 for E1, 373 for E2, 70 for p7, 453 for NS2, and 683 for NS3. Based on sequence and phylogenetic analysis of 5΄-UTR, Npro, and E2 the isolate 297 was characterized as a border disease virus of genotype 3. It was found to be distinct from other BDV-3 strains analyzed so far, consequently forming a distinct branch within the phylogenetic clade. All these data expand a relatively limited knowledge of genetic properties of individual BDV genotypes and strains circulating in the Central Europe.
journal_name
Acta Viroljournal_title
Acta virologicaauthors
Lešková V,Jacková A,Vlasáková M,Vilček Sdoi
10.4149/av_2013_01_17subject
Has Abstractpub_date
2013-01-01 00:00:00pages
17-25issue
1eissn
0001-723Xissn
1336-2305journal_volume
57pub_type
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