Abstract:
:A strain of rickettsiae, designated Crimea-108, was isolated from ticks Dermacentor marginatus in the Crimea in 1977. Its immunobiological characteristics involve low pathogenicity for experimental animals, moderate infectivity for chick embryos, and antigenic relatedness to spotted fever group (SFG) rickettsiae (R. sibirica, R. conorii, R. akari), especially to R. sibirica. The genotypic characterization of the strain Crimea-108 was carried out in comparison with SFG and typhus group rickettsiae by using restriction fragment length polymorphism (RFLP) analysis and DNA-probe hybridization. The marked similarity was detected between DNA restriction patterns of the strains Crimea-108, R. sibirica and R. conorii, but each of them besides comigrating fragments had specific ones. Genotypic analysis of the strain Crimea-108, the SFG and typhus group rickettsiae by three independent DNA probes, based on R. prowazekii DNA, gave unique hybridization patterns for the Crimea-108 strain with all probes. The obtained data show that the Crimea-108 isolate does not belong to the species of R. sibirica, R. conorii, R. akari. The strain Crimea-108 is a novel strain of SFG rickettsiae for the Crimea region.
journal_name
Acta Viroljournal_title
Acta virologicaauthors
Balayeva NM,Demkin VV,Rydkina EB,Ignatovich VF,Artemiev MI,Lichoded LYa,Genig VAsubject
Has Abstractpub_date
1993-12-01 00:00:00pages
475-83issue
6eissn
0001-723Xissn
1336-2305journal_volume
37pub_type
杂志文章相关文献
ACTA VIROLOGICA文献大全abstract::The presence of interferon and type A immunoglobulins (IgA) was followed up in the nasopharyngeal washings collected from volunteers immunized intranasally with an inactivated influenza vaccine [strain A/Rom 1/73 (H3N2)]. Interferon was detected 24 hours after vaccine administration, its incidence being similar to tha...
journal_title:Acta virologica
pub_type: 杂志文章
doi:
更新日期:1975-05-01 00:00:00
abstract::Viraemia was studied in adult Clethrionomys glareolus subcutaneously infected with 12 strains of tick-borne encephalitis (TBE) virus isolated in western and eastern foci of TBE. Nine strains caused viraemia regularly or irregularly, reaching titres higher than the threshold level of TBE virus infectivity for its vecto...
journal_title:Acta virologica
pub_type: 杂志文章
doi:
更新日期:1979-05-01 00:00:00
abstract::Palma and Bhanja bunyaviruses replicated in Dermacentor marginatus, D. reticulatus, Rhipicephalus sanguineus, R. appendiculatus and Ixodes ricinus ticks after parenteral inoculation and appropriate incubation and feeding. Palma virus was transmitted to D. marginatus and D. reticulatus males, D. reticulatus and R. sang...
journal_title:Acta virologica
pub_type: 杂志文章
doi:
更新日期:1997-12-01 00:00:00
abstract::The techniques of reverse transcription-polymerase chain reaction (RT-PCR) and restriction analysis were used to differentiate highly virulent Indian field isolates of infectious bursal disease virus (IBDV) from vaccine strains. Primers were designed to amplify the variable region of VP2 gene coding for major virus ne...
journal_title:Acta virologica
pub_type: 杂志文章
doi:
更新日期:1999-08-01 00:00:00
abstract::Incubation of Aedes albopictus cells infected with Mayaro virus at 37 degrees C causes inhibition of virus replication. During the first hour post infection (p.i.) incubation at 37 degrees C inhibited cellular and virus proteosynthesis. A preferential translation of heat shock proteins 82 kD and 70 kD was observed. Af...
journal_title:Acta virologica
pub_type: 杂志文章
doi:
更新日期:1989-05-01 00:00:00
abstract::Interferon (IFN) was induced in chicken peripheral blood leukocytes by exposure to Concanavalin A (Con A). It has reached a maximum level 96 hr after stimulation with the optimum dose of 10 micrograms/ml. The IFN was partially purified by chromatography on controlled-pore glass adsorbent and was characterized as a fai...
journal_title:Acta virologica
pub_type: 杂志文章
doi:
更新日期:1986-03-01 00:00:00
abstract::Herpes simplex virus 1 (HSV-1) is one of the most prevalent human pathogens in both industrialized and developing countries. This study was performed to analyze the antiviral activity of purified flavonoid from Polygonum perfoliatum L. against HSV-1 infection in vitro and in vivo. Flavonoid showed no inhibitory effect...
journal_title:Acta virologica
pub_type: 杂志文章
doi:10.4149/av_2014_04_368
更新日期:2014-01-01 00:00:00
abstract::Molecular analysis of histological hallmarks (neurofibrillary tangles, neuritic plaques, neuropil threads and dystrophic neurites) of Alzheimer diseased brain tissues showed that these lesions contain paired helical filaments. Their major constituent is microtubule associated protein tau that is in an abnormally hyper...
journal_title:Acta virologica
pub_type: 杂志文章,评审
doi:
更新日期:1994-06-01 00:00:00
abstract::Coxiella burnetii cells in both phase I and II reveal in sodium dodecylsulphate-polyacrylamide gel electrophoresis (SDS-PAGE) similar protein profiles with only small differences. C. burnetii protein profile in SDS-PAGE depended on the method of purification of C. burnetii cells from chick embryo yolk sacs. Immune mou...
journal_title:Acta virologica
pub_type: 杂志文章
doi:
更新日期:1994-10-01 00:00:00
abstract::The cold-adapted temperature-sensitive (ts) influenza virus strain A/Leningrad/134/17/57 (H2N2) multiplied well at 32 degrees C (optimal temperature); lower titres of infectious virus were obtained in developing chick embryos at 40 degrees C. In a canine kidney (MDCK) cell line and in primary calf kidney (CK) cells an...
journal_title:Acta virologica
pub_type: 杂志文章
doi:
更新日期:1982-07-01 00:00:00
abstract::Homologs of Bombyx mori nuclear polyhedrosis virus (BmNPV) Bm67 gene ORF67 have been found in the genome of all lepidopteran nuclear polyhedrosis viruses, but their function is still not very clear. In order to analyze it we employed a bacmid harboring the complete BmNPV genome including the Bm67 gene and expressing ...
journal_title:Acta virologica
pub_type: 杂志文章
doi:10.4149/av_2015_01_40
更新日期:2015-03-01 00:00:00
abstract::Herpes zoster (HZ) is a neurocutaneous disease caused by Varicella-zoster virus (VZV) as a consequence of declined cell-mediated immunity, immune suppression and immunodeficiency. As reactivation of JC polyomavirus (JCPyV) might be linked with immunodeficiency or immunosuppressive therapy, the relationship between HZ ...
journal_title:Acta virologica
pub_type: 杂志文章
doi:
更新日期:2006-01-01 00:00:00
abstract::Homologous interference in vitro is defined as the ability of primary viral infection to prevent secondary homologous superinfection. Non-cytopathic bovine viral diarrhea virus (ncp BVDV) has been classified according to the exaltation of Newcastle disease phenomenon (END) as END positive (E+) and END negative (E-) st...
journal_title:Acta virologica
pub_type: 杂志文章
doi:10.4149/av_2018_228
更新日期:2018-01-01 00:00:00
abstract::The sensitivity of enzyme linked immunoassay (ELISA) was compared to that of haemagglutination inhibition (HI) test in detection of humoral antibodies in birds vaccinated with Newcastle disease virus (NDV). The highest antibody titre at day 21 post-vaccination as detected by ELISA in the sera of vaccinated birds was 2...
journal_title:Acta virologica
pub_type: 杂志文章
doi:
更新日期:1985-03-01 00:00:00
abstract::Recently, mitochondria have been shown to have a vital role in the innate immune response to viral infection. In response, viruses such as adenovirus, have developed mechanisms to alter mitochondrial function through direct or indirect interaction with the mitochondria. The interaction of human recombinant adenoviral ...
journal_title:Acta virologica
pub_type: 杂志文章
doi:10.4149/av_2019_114
更新日期:2019-01-01 00:00:00
abstract::The effect of some cellular function inhibitors on adsorption and successive events of penetration of vesicular stomatitis virus to phylogenetically unrelated permissive cells was investigated. Treatment of HeLa, CER, EPC and Aedes albopictus cells with colcemide and cytochalasin D which affect cytoskeleton organizati...
journal_title:Acta virologica
pub_type: 杂志文章
doi:
更新日期:1988-11-01 00:00:00
abstract::An assay protocol based on exploiting the polymerase chain reaction (PCR) for the direct detection of influenza B virus in throat swabs is described. By the use of PCR with nested primers, it was possible to detect the virus in throat swabs. Dilution experiments showed that as little as 1 plaque forming unit of virus ...
journal_title:Acta virologica
pub_type: 杂志文章
doi:
更新日期:1992-05-01 00:00:00
abstract::Tick-borne encephalitis (TBE), a disease caused by tick-borne encephalitis virus (TBEV), represents a serious neural infection of humans in Europe and Asia. The main reservoir hosts of TBEV are ticks, rodents and insectivores, but domestic animals may also be infected with the virus. This review summarizes what is kno...
journal_title:Acta virologica
pub_type: 杂志文章
doi:10.4149/av_2020_212
更新日期:2020-01-01 00:00:00
abstract::Human papillomavirus (HPV) E6 and E7 gene products play a central role in the induction of benign proliferation and malignant transformation by interacting with several cellular regulatory proteins such as p53, p16(INK4a), and nuclear factor kappaB (NF-kappaB). In this study, HPV DNA was detected by in situ hybridizat...
journal_title:Acta virologica
pub_type: 杂志文章
doi:10.4149/av_2009_01_43
更新日期:2009-01-01 00:00:00
abstract::RNA interference (RNAi) is a biological phenomenon in which introduction of a small, double-stranded interfering RNAs (siRNAs) into a cell causes a specific degradation of homologous single-stranded RNA. siRNA can be delivered into the cell by different approaches including synthetic RNA, in vitro transcribed RNA and ...
journal_title:Acta virologica
pub_type: 杂志文章
doi:
更新日期:2005-01-01 00:00:00
abstract::Immobilization stress (hypokinesis) in Balb/c mice may aggravate asymptomatic infection with Langat virus (strain TP-21) as evidenced by 4-fold increased lethality in comparison with control animals. The virus levels in the spleen and brain of stressed and infected mice and the in vitro yield of the virus in immunocom...
journal_title:Acta virologica
pub_type: 杂志文章
doi:
更新日期:1990-05-01 00:00:00
abstract::ORF 29 of Bombyx mori nucleopolyhedrovirus (BmNPV ORF29), a conserved gene among all the lepidopteran baculoviruses, was subjected to analysis of sequence and transcription/translation in the insect BmN cells in vitro. His-tagged BmNPV ORF29 fusion protein was expressed in Escherichia coli, purified and used for the p...
journal_title:Acta virologica
pub_type: 杂志文章
doi:10.4149/av_2010_04_275
更新日期:2010-01-01 00:00:00
abstract::Among diseases transmitted by ticks in the Czech Republic, tick-borne encephalitis (TBE) caused by Tick-borne encephalitis virus (TBEV) and Lyme disease caused by Borrelia burgdorferi spirochete are most important. We propose an effective and specific test for detection of TBEV in a single tick or a pool of ticks base...
journal_title:Acta virologica
pub_type: 杂志文章
doi:
更新日期:2004-01-01 00:00:00
abstract::This study reports on our experience with whole herpes simplex vaccines in Bulgaria for a period of 17 years. More than 1,500 immunized patients with herpes ophtalmicus showed a reduction of the recovery period, reduced number of the recurrences and reduced risk of visual damage. More than 14,000 patients suffering fr...
journal_title:Acta virologica
pub_type: 临床试验,杂志文章
doi:
更新日期:1994-08-01 00:00:00
abstract::Human influenza virus A/Krasnodar/101/59 (H2N2) was passaged in chick fibroblast cultures in the presence of trypsin at suboptimal temperature. The virus which underwent 16 passages at 28 degrees C possessed cold-adapted (ca) and temperature sensitive (ts) phenotypes and formed larger plaques at the optimal temperatur...
journal_title:Acta virologica
pub_type: 杂志文章
doi:
更新日期:1984-09-01 00:00:00
abstract::An atypical mild isolate HV5 of Grapevine fanleaf virus (GFLV) was found in a South Moravian viticulture region in Czech Republic. Partial sequence of its RNA2 was determined and compared with available sequences of typical GFLV isolates. Two genomic regions, namely a 814 nt-long one spanning the movement protein (MP)...
journal_title:Acta virologica
pub_type: 杂志文章
doi:
更新日期:2006-01-01 00:00:00
abstract::The plaque size and distribution of prototype La Crosse (LAC) and Jamestown Canyon (JC) viruses were investigated in Vero cell cultures. The effect of serial passage of the viruses in their natural vertebrate hosts - the chipmunk and grey squirrel for LAC and the white-tailed deer for JC virus - was studied. Prototype...
journal_title:Acta virologica
pub_type: 杂志文章
doi:
更新日期:1975-07-01 00:00:00
abstract::An analysis of the methods of testing the antiviral activity in vitro (in cell cultures) and in vivo (in experimental virus infections) is presented. The necessity of applying two-stage screening in vitro is emphasized. The chemotherapeutic viral models is in vitro testing as well as the principles of modelling of exp...
journal_title:Acta virologica
pub_type: 杂志文章
doi:
更新日期:1978-07-01 00:00:00
abstract::Monocytes/macrophages have been known to play an important role in the initiation and propagation of human immunodeficiency virus 1 (HIV-1) infection. To analyze the function of these cells during the clinical asymptomatic period of infection, we examined the effect of murine peritoneal macrophages and human periphera...
journal_title:Acta virologica
pub_type: 杂志文章
doi:
更新日期:1997-02-01 00:00:00
abstract::Antibodies against (a) naked particles, (b) virus envelope, and (c) whole herpes simplex virus (HSV) type 1 were investigated. Immune rabbit serum against naked particles (and formalized naked particles) contained virus neutralizing (VN) antibody in a low titre as compared to the titre of complement-fixing (CF) antibo...
journal_title:Acta virologica
pub_type: 杂志文章
doi:
更新日期:1976-02-01 00:00:00