Abstract：
:The uses of the nonionic detergent Triton X-100 (TX-100) and the cationic cetyltrimethylammonium bromide (CTAB) for the production of tick-borne encephalitis (TBE) virus subunits were compared. TX-100 splitted the virus into haemagglutinating envelope components which contain the virion proteins V3 and V1 and into a lipid-free nucleoprotein composed of RNA and V2. The lipid content of the released envelope fragments decreased with increasing detergent concentration. By the use of CTAB, the viral glycoprotein V3 could be prepared in electrophoretically pure and active, i.e. haemagglutinating form.
journal_name
Acta Viroljournal_title
Acta virologicaauthors
Heinz F,Kunz Csubject
Has Abstractpub_date
1979-05-01 00:00:00pages
189-97issue
3eissn
0001-723Xissn
1336-2305journal_volume
23pub_type
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