Abstract：
:Mouse sera collected from day 4 to day 133 postinfection (p.i.) with phase I Coxiella burnetii strain Nine Mile were analysed by immunoblotting with phase I C. burnetii cell lysate. Antibodies of IgG class protein antigens were revealed already on day 10 p.i. (60, 49 and 27 kD proteins), followed by those to 77 kD protein from day 18 p.i. and to further 7 (from 42 to 70 kD) proteins from day 28 p.i. IgG antibody reaction was observed also with 5 antigens in 14-20 kD region corresponding to lipopolysaccharides from day 22 p.i. Surprisingly, antibodies of IgM type appeared later (from day 22 p.i.) and were directed only to protein antigens, most markedly to 60 and 77 kD proteins. Differences in immunoblot patterns observed with the serum collected on day 72 p.i. before and after absorption to phase I and phase II C. burnetii cells, and to phase I cells treated by trichloroacetic acid (TCA) or KIO4, indicate the surface localization of protein phase I antigenic epitopes, which can be destroyed partly by TCA and almost completely by KIO4 treatment.
journal_name
Acta Viroljournal_title
Acta virologicaauthors
Novák M,Brezina R,Kazár Jsubject
Has Abstractpub_date
1992-01-01 00:00:00pages
39-44issue
1eissn
0001-723Xissn
1336-2305journal_volume
36pub_type
杂志文章相关文献
ACTA VIROLOGICA文献大全abstract：:A comparative polarographic study on the alkaline degradation of tobacco mosaic virus (TMV) strain vulgare and its mutant TMV 483, having histidine instead of glutamine at position 9 in the polypeptide chain, was performed. In the course of alkaline degradation and subsequent incubation in the supporting electrolyte a...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1977-09-01 00:00:00
abstract：:Tick-borne encephalitis (TBE), a disease caused by tick-borne encephalitis virus (TBEV), represents a serious neural infection of humans in Europe and Asia. The main reservoir hosts of TBEV are ticks, rodents and insectivores, but domestic animals may also be infected with the virus. This review summarizes what is kno...
journal_title：Acta virologica
pub_type： 杂志文章
doi：10.4149/av_2020_212
更新日期：2020-01-01 00:00:00
abstract：:A region of the UL24 gene of six Australian field isolates of Bovine herpesvirus 2 (BHV-2) was sequenced after a passage in Madin-Darby bovine kidney (MDBK) cells by polymerase chain reaction (PCR). While the PCR product covered the first half of the UL24 gene, a particular interest was focused on the 274-297 nucleoti...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：2002-01-01 00:00:00
abstract：:An atypical mild isolate HV5 of Grapevine fanleaf virus (GFLV) was found in a South Moravian viticulture region in Czech Republic. Partial sequence of its RNA2 was determined and compared with available sequences of typical GFLV isolates. Two genomic regions, namely a 814 nt-long one spanning the movement protein (MP)...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：2006-01-01 00:00:00
abstract：:Cytomegalovirus (CMV) antibody was detected in human sera by the gel precipitin test. The results were closely related to those obtained by complement fixation. The simplicity of the test makes it useful for the routine serological diagnosis of CMV infection. ...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1978-01-01 00:00:00
abstract：:Cauliflower mosaic virus (CaMV) - a plant pararetrovirus that naturally causes diseases in Brassicaceae and Solanaceae plant hosts worldwide - has been detected by PCR for the first time in herbarium samples of Usnea sp. lichens. The virus's presence in these lichens did not result in any micro- or macromorphological ...
journal_title：Acta virologica
pub_type： 杂志文章
doi：10.4149/av_2016_02_196
更新日期：2016-06-01 00:00:00
abstract：:Differences in interfering activities were demonstrated between low-passage epidemic and thermosensitive cold-adapted strains of influenza viruses A and B in experiments on chick embryos inoculated on the chorioallantoic membranes and on white mice. At a high multiplicity of infection the thermosensitive variants show...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1982-05-01 00:00:00
abstract：:Cytotoxic T lymphocyte (CTL) activity of draining lymph node (DLN) cells isolated from BALB/c mice infected with ectromelia virus (EV) was examined using a fluorometric cell-mediated cytotoxicity (CMC) assay. Specific lysis of target cells A20 and EMT-6 primed with EV was demonstrated. The classical CD8+ cytolytic pat...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1996-11-01 00:00:00
abstract：:The coat proteins of both ordinary (PVSO) and Andean (PVSA) strains were shown to be similar in molecular weights, peptide maps, amino acid analysis and reaction to a range of carlavirus antiseras. ...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1992-03-01 00:00:00
abstract：:The sensitivity of enzyme linked immunoassay (ELISA) was compared to that of haemagglutination inhibition (HI) test in detection of humoral antibodies in birds vaccinated with Newcastle disease virus (NDV). The highest antibody titre at day 21 post-vaccination as detected by ELISA in the sera of vaccinated birds was 2...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1985-03-01 00:00:00
abstract：:A noninfectious soluble antigen fraction of goat poxvirus (GPV) fractionated by ammonium sulfate precipitation was tested for its suitability as coating antigen in an indirect enzyme-linked immunosorbent assay (ELISA). Accordingly, an avidin-biotin ELISA for the detection of GPV antibodies was optimized and evaluated ...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1999-10-01 00:00:00
abstract：:The level of the Epstein-Barr virus (EBV)-determined nuclear antigen (EBNA-1) encoded by the Bam HI-K fragment of EBV DNA remained unchanged in P3HR-1 cells after induction of the productive cycle of virus replication by sodium-n-butyrate and 12-o-tetradecanoylphorbol-13-acetate (TPA). This was shown by the same capac...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1988-07-01 00:00:00
abstract：:The prevalence of hepatitis C virus (HCV) infection in the population of human immunodeficiency virus 1 (HIV-1)-infected individuals from Slovenia and Croatia was determined. One hundred and sixty-six out of a total of 188 Slovenian HIV-1-infected individuals and 120 subjects who were randomly chosen out of a total 34...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：2002-01-01 00:00:00
abstract：:Neopterin and soluble CD14 (sCD14) are detected at high levels in hepatitis C virus (HCV) infections. We aimed to evaluate the role of these plasma immune activation biomarkers, for the indirect assessment of immune activation status of patients with low anti-HCV reactivity and a HCV infection. Low anti-HCV reactivity...
journal_title：Acta virologica
pub_type： 杂志文章,多中心研究
doi：10.4149/av_2017_304
更新日期：2017-01-01 00:00:00
abstract：:ORF 29 of Bombyx mori nucleopolyhedrovirus (BmNPV ORF29), a conserved gene among all the lepidopteran baculoviruses, was subjected to analysis of sequence and transcription/translation in the insect BmN cells in vitro. His-tagged BmNPV ORF29 fusion protein was expressed in Escherichia coli, purified and used for the p...
journal_title：Acta virologica
pub_type： 杂志文章
doi：10.4149/av_2010_04_275
更新日期：2010-01-01 00:00:00
abstract：:Over a 5-year observation period in a children-institution community, the severity of respiratory-syncytial (RS) virus infection varied within a broad range depending on biological properties of the circulating virus population. The type of virus population was characterized by a complex of biological features, namely...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1985-07-01 00:00:00
abstract：:Effects of certain groups of nutrients such as glucose, essential amino acids (AA), nonessential AA, vitamins and trace nutrients on the multiplication of various strains of Japanese encephalitis virus (JEV) were studied with an aim to optimise the conditions for cultivation of the virus in porcine stable (PS) kidney ...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1995-12-01 00:00:00
abstract：:Histology of skin lesions and demonstration in them of Rickettsia conorii by direct immunofluorescence test (DIF) are presented in 13 patients with Mediterranean spotted fever (MSF). The lymphohistiocytic vasculitis which dominated the picture is not specific, however, it could be suggestive for the diagnosis of ricke...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1991-11-01 00:00:00
abstract：:We present serological evidence of infection with western and eastern type of haemorrhagic fever with renal syndrome (HFRS) virus in patient's sera from Slovakia by indirect immunofluorescence. Treatment of sera with 2-mercaptoethanol decreased levels of haemagglutination-inhibition (HI) antibodies to the eastern type...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1990-08-01 00:00:00
abstract：:Five H5N2 avian influenza viruses (AIVs) were isolated in Xianghai National Nature Reserve, Jilin Province, China in October 2011. They were all identified as low pathogenic AIVs (LPAIVs) based on their deduced amino acid sequences at the cleavage site of HA protein. Phylogenetic analysis revealed that all gene segmen...
journal_title：Acta virologica
pub_type： 杂志文章
doi：10.4149/av_2015_02_140
更新日期：2015-06-01 00:00:00
abstract：:The uses of the nonionic detergent Triton X-100 (TX-100) and the cationic cetyltrimethylammonium bromide (CTAB) for the production of tick-borne encephalitis (TBE) virus subunits were compared. TX-100 splitted the virus into haemagglutinating envelope components which contain the virion proteins V3 and V1 and into a l...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1979-05-01 00:00:00
abstract：:Human influenza virus A/Krasnodar/101/59 (H2N2) was passaged in chick fibroblast cultures in the presence of trypsin at suboptimal temperature. The virus which underwent 16 passages at 28 degrees C possessed cold-adapted (ca) and temperature sensitive (ts) phenotypes and formed larger plaques at the optimal temperatur...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1984-09-01 00:00:00
abstract：:We characterized leukocytes in peripheral blood of BALB/c mice infected with mouse herpesvirus isolate 72 (MHV-72) representing an isolate of mouse herpesvirus strain 68 (MHV-68, species Murid herpesvirus 4, genus Rhadinovirus, subfamily Gammaherpesvirinae, family Herpesviridae) (van Regenmortel et al., 2000). In acut...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：2002-01-01 00:00:00
abstract：:Protective effects of different types of Q fever vaccines, namely untreated Coxiella burnetii phase I cells (Cb I) or Cb I cells treated with chloroform-methanol (CM) mixture (Cb I-CM) and of a Q fever chemovaccine obtained by trichloroacetic acid extraction (TCAE) from intact Cb I cells, were compared in mice and gui...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1986-11-01 00:00:00
abstract：UNLABELLED:Antigenic and genetic typing of pestiviruses isolated from Indian sheep and goats was carried out. Testing of 1777 sheep and 1026 goat blood samples collected between 2004 and 2008 resulted in isolation of twelve pestiviruses, seven from sheep and five from goats. All of them were antigenically typed as bovi...
journal_title：Acta virologica
pub_type： 杂志文章
doi：10.4149/av_2012_03_209
更新日期：2012-01-01 00:00:00
abstract：:Among diseases transmitted by ticks in the Czech Republic, tick-borne encephalitis (TBE) caused by Tick-borne encephalitis virus (TBEV) and Lyme disease caused by Borrelia burgdorferi spirochete are most important. We propose an effective and specific test for detection of TBEV in a single tick or a pool of ticks base...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：2004-01-01 00:00:00
abstract：:In order to determine the definitive importance of T- and B-lymphocytes in immunity to Rickettsia conorii, mice genetically deficient in T-cells, B-cells, or both T- and B-cells were infected experimentally. T-lymphocytes rather than humoral antibodies were crucial to rickettsial clearance and a reduced mortality rate...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1984-11-01 00:00:00
abstract：:The genome sequence of a novel species of the genus Waikavirus (the family Secoviridae), which we named Brassica napus RNA virus 1 (BnRV1), was identified in a rapeseed (Brassica napus) transcriptome dataset. The BnRV1 genome was 12,293 nucleotides long followed by a poly(A) tail. Two open reading frames (ORFs), calle...
journal_title：Acta virologica
pub_type： 杂志文章
doi：10.4149/av_2019_205
更新日期：2019-01-01 00:00:00
abstract：:In postnatally acquired lymphocytic choriomeningitis (LCM), specific immunofluorescent (IF) IgM antibody was found nearly in all the examined sera from 23 patients for 6-8 months after onset of the disease, IgA antibody in two-thirds of the patients and only during the first 5 weeks of the disease. In the first weeks,...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1978-05-01 00:00:00
abstract：:This study reports on our experience with whole herpes simplex vaccines in Bulgaria for a period of 17 years. More than 1,500 immunized patients with herpes ophtalmicus showed a reduction of the recovery period, reduced number of the recurrences and reduced risk of visual damage. More than 14,000 patients suffering fr...
journal_title：Acta virologica
pub_type： 临床试验,杂志文章
doi：
更新日期：1994-08-01 00:00:00
