Nutrient composition for cultivation of Japanese encephalitis virus in vitro.

Abstract:

:Effects of certain groups of nutrients such as glucose, essential amino acids (AA), nonessential AA, vitamins and trace nutrients on the multiplication of various strains of Japanese encephalitis virus (JEV) were studied with an aim to optimise the conditions for cultivation of the virus in porcine stable (PS) kidney cell cultures. Eagle's Minimal Essential Medium (MEM) was modified by addition of the nutrients in different concentrations and combinations. Glucose was found the most important single nutrient in promoting significantly the virus multiplication. Essential AA alone did not influence the virus yield, while in combination with glucose they caused its marked increase. Vitamins and other nutrients did not stimulate significantly virus multiplication. The study revealed that the extent of the glucose effect depends on the virus strain used.

journal_name

Acta Virol

journal_title

Acta virologica

authors

Kadam VD,Paranjape SP,Gangodkar SV,Deolankar RP

subject

Has Abstract

pub_date

1995-12-01 00:00:00

pages

287-9

issue

5-6

eissn

0001-723X

issn

1336-2305

journal_volume

39

pub_type

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