Abstract：
:The techniques of reverse transcription-polymerase chain reaction (RT-PCR) and restriction analysis were used to differentiate highly virulent Indian field isolates of infectious bursal disease virus (IBDV) from vaccine strains. Primers were designed to amplify the variable region of VP2 gene coding for major virus neutralizing epitopes. The 552 bp PCR products generated from four vaccine strains and five field isolates were digested with restriction enzymes DraI, HhaI, MvaI, StuI, StyI, and TaqI, which could differentiate field isolates from vaccine strains. Based on restriction enzyme profiles derived from published sequences, Indian field isolates seem to be closely related to highly virulent Japanese, European, and Chinese strains of the virus.
journal_name
Acta Viroljournal_title
Acta virologicaauthors
Kataria RS,Tiwari AK,Butchaiah G,Kataria JMsubject
Has Abstractpub_date
1999-08-01 00:00:00pages
245-9issue
4eissn
0001-723Xissn
1336-2305journal_volume
43pub_type
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