Abstract:
:Virus-specific structures with sedimentation coefficients of 250-300, 200 and 150 S were isolated from the polysome fraction of Sendai virus-infected Ahrlich ascitic carcinoma cells treated with cycloheximide, at early stages of infection (1.5 to 2 hours after inoculation). All these 3 types of structure contained both parental and newly synthesized viral RNA. RNA extracted from these structures consisted of 2 components sedimenting in sucrose density gradients in the zones of 50-70 and 35-40 S. Both components contained parental and newly synthesized RNA and were partially resistant to ribonuclease. RNA extracted from rapidly sedimenting structures (250-300 S) contained mainly the 50-70 S component; RNA recovered from 200 S structures contained the 35-40 S component. By analogy with reported data, the isolated forms of RNA have been characterized as transcriptive intermediates.
journal_name
Acta Viroljournal_title
Acta virologicaauthors
Vladimirtseva EA,Bakunts KA,Lebedeva EN,Bukrinskaya AGsubject
Has Abstractpub_date
1975-09-01 00:00:00pages
374-80issue
5eissn
0001-723Xissn
1336-2305journal_volume
19pub_type
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