Optimum conditions for the storage of potato virus S.

Abstract:

:The effect of storage conditions on the serological activity of two strains of potato virus S (PVS), Andean and the ordinary, was studied by ELISA. Virus purificates, infected leaves and their homogenates, stored in lyophilized, frozen and dissolved form at various temperatures were tested. Virus purificates were most stable in lyophilized form, their activity decreased after 9 months only by 20-30%. Also non-purified virus was most stable as a lyophilized leaf homogenate, its activity decreased after 12 months by 30%. When lyophilized leaves were stored, the virus activity dropped after 12 months by 45%. Both the Andean and the ordinary strain of PVS behaved similarly during storage under the conditions tested.

journal_name

Acta Virol

journal_title

Acta virologica

authors

Cerovská N,Filigarová M

subject

Has Abstract

pub_date

1995-02-01 00:00:00

pages

51-2

issue

1

eissn

0001-723X

issn

1336-2305

journal_volume

39

pub_type

杂志文章
  • Evaluation of avidin-biotin ELISA for the detection of antibodies to goat poxvirus using noninfectious diagnostic reagent.

    abstract::A noninfectious soluble antigen fraction of goat poxvirus (GPV) fractionated by ammonium sulfate precipitation was tested for its suitability as coating antigen in an indirect enzyme-linked immunosorbent assay (ELISA). Accordingly, an avidin-biotin ELISA for the detection of GPV antibodies was optimized and evaluated ...

    journal_title:Acta virologica

    pub_type: 杂志文章

    doi:

    authors: Rao TV,Malik P,Asgola D

    更新日期:1999-10-01 00:00:00

  • Protective efficacy of IFN-ω AND IFN-λs against influenza viruses in induced A549 cells.

    abstract::The interferon system represents one of the components of the first line defence against influenza virus infection. Interferon omega (IFN-ω) is antigenetically different from IFN-α and IFN-β and can affect patients who are resistant to these IFNs. To improve the biological characterization of IFN-ω, we compared its ac...

    journal_title:Acta virologica

    pub_type: 杂志文章

    doi:10.4149/av_2015_04_413

    authors: Škorvanová L,Švančarová P,Svetlíková D,Betáková T

    更新日期:2015-12-01 00:00:00

  • Cross-reactive killer T lymphocytes in a flavivirus infection.

    abstract::Splenocytes from mice immunized intravenously with one dose of Langat or Yellow fever viruses were cytotoxic for target cells infected with virulent tick-borne encephalitis (TBE) virus; cytotoxicity was measured by 51Cr release from target L929 cells. No significant lysis of the TBE virus-infected taget cells with spl...

    journal_title:Acta virologica

    pub_type: 杂志文章

    doi:

    authors: Gajdosová E,Mayer V,Oravec C

    更新日期:1980-06-01 00:00:00

  • Escape mutants of foot-and-mouth disease virus selected by monoclonal antibodies directed to a trypsin-sensitive neutralization epitope.

    abstract::Five monoclonal antibodies (MoAbs) against Indian reference/vaccine strain of foot-and-mouth disease (FMD) virus subtype A22 (IND17/77) and a guinea pig antibody against a synthetic peptide representing amino acids (aa) 136-151 of VP1 polypeptide of A22 virus were used in the study. All the antibodies either failed to...

    journal_title:Acta virologica

    pub_type: 杂志文章

    doi:

    authors: Das BR,Pattnaik B,Venkataramanan R,Rai DV

    更新日期:1997-06-01 00:00:00

  • An enzyme-linked immunosorbent assay (ELISA) for measurement of antibodies against equine herpesvirus 2 in equine sera.

    abstract::An indirect enzyme-linked immunosorbent assay (ELISA) was developed for the detection of antibodies against equine herpesvirus type 2 (EHV-2) in equine sera. The optimal conditions of antigen concentration, and serum and conjugate dilutions were established by chequerboard titrations. When the standard ELISA test was ...

    journal_title:Acta virologica

    pub_type: 杂志文章

    doi:

    authors: Fu ZF,Denby L,Lien DH,Robinson AJ

    更新日期:1987-11-01 00:00:00

  • Hepatitis B surface antigen/IgM complexes: relation to receptors for polymerized human serum albumin, hepatitis B virus (HBV) DNA polymerase activity and HBV markers.

    abstract::The presence and the level of hepatitis B surface antigen (HBsAg)/IgM complexes were determined in 54 chronic HBsAg carriers in relation to receptors for polymerized human serum albumin (pHSA-R) tested by specific radioimmunoassay, and to hepatitis B virus-DNA polymerase (HBV-DNAp). HBsAg/IgM complexes, correlated sig...

    journal_title:Acta virologica

    pub_type: 杂志文章

    doi:

    authors: Mora I,Carreño V,Porres JC,Gutiez J,Guio CH

    更新日期:1987-03-01 00:00:00

  • Inhibition of expression of rna polymerase with small interfering RNAs targeting a conserved motif in the respective viral genes in viruses of the family Flaviviridae.

    abstract::The RNA-dependent RNA polymerase (RdRp) in viruses of the family Flaviviridae plays an important role in the viral replication process and in the forming of a replicase complex. We used small interfering RNAs (siRNAs) corresponding to the highly conservative Motif V of RdRp gene of different viruses to examine their r...

    journal_title:Acta virologica

    pub_type: 杂志文章

    doi:

    authors: Xu Z,Li X,Liu R,Si Y,Sun M,Jin M,Chen H,Qian P

    更新日期:2007-01-01 00:00:00

  • Autoantibodies in sera of influenza patients.

    abstract::Sera of the influenza patients and healthy controls were tested for some types of autoantibody (SMA, ANA, ABBA, AMA). They were detected in 83.8% of the patients' sera and in 16.6% of controls. SMA were present in 77.4%, ANA in 54.8%, and ABBA in 16.1% of the patient's sera. AMA were not detected. A majority of the se...

    journal_title:Acta virologica

    pub_type: 杂志文章

    doi:

    authors: Loza-Tulimowska M,Semkow R,Michalak T,Nowoslawski A

    更新日期:1976-06-01 00:00:00

  • Hepatitis B vaccine alone or in combination with anti-HBs immunoglobulin in the perinatal prophylaxis of babies born to HBsAg carrier mothers.

    abstract::The efficacy of hepatitis B virus (HBV) vaccine alone (group I) or in combination with hepatitis B immunoglobulin (HBIG) (group II) for prevention of perinatal transmission of the virus was assessed in 21 and 24 neonates, respectively. 58 infants who could not be vaccinated constituted the control group. It was observ...

    journal_title:Acta virologica

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:

    authors: Sehgal A,Gupta I,Sehgal R,Ganguly NK

    更新日期:1992-08-01 00:00:00

  • Effects of monoparacoumarylputrescinium chloride on the hypersensitive reaction of Gomphrena globosa leaves to tomato bushy stunt virus.

    abstract::Monoparacoumarylputrescinium chloride (pCPH), supplied to detached leaves of Gomphrena globosa via the petiole, induced interference with tomato bushy stunt virus infection by reducing the number and size of the necrotic local lesions. The phenolic compound neither inactivated directly the virus in vitro, nor induced ...

    journal_title:Acta virologica

    pub_type: 杂志文章

    doi:

    authors: Pennazio S,Dellamonica G,Redolfi P

    更新日期:1981-03-01 00:00:00

  • Correlation between molecular size and interferon- inducing activity of poly I:C.

    abstract::Electron microscopy showed that commerical poly I: C consisted of molecules varying in length from less than 0.05 nm to more than 5 nm and also in morphology . To clarify the relationship between its molecular size and interferon-inducing activity, poly I: C was sonicated or fractionated by sucrose density gradient ce...

    journal_title:Acta virologica

    pub_type: 杂志文章

    doi:

    authors: Arimura H

    更新日期:1975-11-01 00:00:00

  • Targeting microRNA-122: walking on cutting edge of hepatitis C virus infection therapy.

    abstract::Hepatitis C virus (HCV) infection, with an estimated 170 million carriers worldwide, remains a major cause of chronic liver disease. Current anti-HCV treatments have significant side effects and have met with only partial success. Therefore, a more effective therapeutic modality for HCV infection is needed. The stabil...

    journal_title:Acta virologica

    pub_type: 杂志文章,评审

    doi:10.4149/av_2014_04_301

    authors: Motavaf M,Safari S,Alavian SM

    更新日期:2014-01-01 00:00:00

  • Cell-mediated immune reactions in measles.

    abstract::Cell-mediated reactions in measles cases, in direct contacts and healthy children were tested. Indices of phytohaemagglutinin-stimulated blastic transformation and leukocyte migration inhibition were evaluated. Positive reactions were found in the first and second weeks after manifestation of clinical symptoms. The ap...

    journal_title:Acta virologica

    pub_type: 杂志文章

    doi:

    authors: Kańtoch M,Abramow-Newerly W,Jankowski M,Imbs D,Litwińska B,Stepień R,Slowińska I,Kubica J,Boguslawska J

    更新日期:1980-03-01 00:00:00

  • Virus-induced decrease in the activity of serum blocking factor(s): a mechanism of activation of the autoreactive T effectors in graft versus host reaction and of controlling T suppressors.

    abstract::It has been shown that serum blocking factor(s) (SBF) previously detected in normal mice bind(s) to receptors on the surface membranes of virus-induced autoreactive T lymphocytes (ARTL) and to that of ARTL activity inhibiting T suppressors (TSar). The interaction of SBF with the receptors is reversible, H-2 restricted...

    journal_title:Acta virologica

    pub_type: 杂志文章

    doi:

    authors: Khozinsky VV,Semenov BF

    更新日期:1985-01-01 00:00:00

  • Live tissue culture influenza vaccine for oral administration. I. Specific immune response to monovaccine A (H3N2) in volunteers.

    abstract::Oral immunization of volunteers with a live tissue culture influenza A (H3N2) vaccine induced an increase in virus neutralizing (VN), haemagglutination inhibiting (HI) and neuraminidase inhibiting (NI) antibody. The dynamics of antibody and IgM and IgG immunoglobulin formation in the blood depended to a great extent o...

    journal_title:Acta virologica

    pub_type: 杂志文章

    doi:

    authors: Alekseeva AK,Andzhaparidze OG,Gracheva EN,Sidorova LA,Melnikova SK

    更新日期:1979-01-01 00:00:00

  • Enzyme-immunoassay in the diagnosis of human hepatitis A: specific and non-specific reactions.

    abstract::Solid-phase enzyme-immunoassay (EIA) used for the detection of hepatitis A virus (HAV) often reveals a non-specific activity which can be reduced or fully eliminated in the presence of normal serum. The factor responsible for this activity appeared to be a non-viral EIA-active material (NVEAM) that non-specifically re...

    journal_title:Acta virologica

    pub_type: 杂志文章

    doi:

    authors: Tolskaya EA,Savinskaya SS,Balayan MS,Andzhaparidze AG,Kolesnikova MS

    更新日期:1983-01-01 00:00:00

  • A virus-inducible cucumber anionic peroxidase has a serological counterpart in different plant species.

    abstract::A highly specific rabbit antiserum raised against anionic peroxidase (PRX) purified from cucumber (Cucumis sativus L., cv. Laura) cotyledons infected with tobacco necrosis virus (TNV) was used to further investigate the antigenic relatedness of peroxidases in the selected plant species. Immunoblot analysis using nativ...

    journal_title:Acta virologica

    pub_type: 杂志文章

    doi:

    authors: Repka V

    更新日期:1996-06-01 00:00:00

  • Malvastrum yellow vein Yunnan virus is amonopartite begomovirus.

    abstract::Nine samples of diseased Malvastrum coromandelianum plants collected from the fields in Yunnan province of China were found to be infected with Malvastrum yellow vein Yunnan virus (MYVYNV), when tested by PCR using specific primers. The results of PCR and Southern blot analysis showed that only 4 samples out of 9 were...

    journal_title:Acta virologica

    pub_type: 杂志文章

    doi:10.4149/av_2010_01_21

    authors: Jiang T,Liu P,Liao BL,Wu JX,Huang CJ

    更新日期:2010-01-01 00:00:00

  • Oligonucleotide fingerprint analysis on Japanese encephalitis virus strains after passage histories.

    abstract::Genetic variation in Japanese encephalitis (JE) virus strains after various passage histories was examined by oligonucleotide fingerprints of the 42S genome RNA. The results indicated that genome changes occurred during passages of new virus isolates through cell lines or mouse brains, especially at earlier passage le...

    journal_title:Acta virologica

    pub_type: 杂志文章

    doi:

    authors: Hori H,Igarashi A,Yoshida I,Takagi M

    更新日期:1986-09-01 00:00:00

  • Oxygen tension and virus replication.

    abstract::An evidence is accumulating that the oxygen tension exerts significant effect on the virus replication in vitro. When the in vitro oxygen tension is maintained at an in vivo physiological level, as a rule higher yields of human viruses are seen that at conventional culturing with access of an unphysiologically high ox...

    journal_title:Acta virologica

    pub_type: 杂志文章,评审

    doi:

    authors: Ebbesen P,Zachar V

    更新日期:1998-12-01 00:00:00

  • Correlation between reproductive and interfering activities of epidemic and thermosensitive influenza virus strains.

    abstract::Differences in interfering activities were demonstrated between low-passage epidemic and thermosensitive cold-adapted strains of influenza viruses A and B in experiments on chick embryos inoculated on the chorioallantoic membranes and on white mice. At a high multiplicity of infection the thermosensitive variants show...

    journal_title:Acta virologica

    pub_type: 杂志文章

    doi:

    authors: Aleksandrova GI,Dekhtyareva NI,Medvedeva TE,Shneider MA

    更新日期:1982-05-01 00:00:00

  • Quantitative and rapid interference of bovine viral diarrhea virus BVDV/END- and BVDV/END+ strains.

    abstract::Homologous interference in vitro is defined as the ability of primary viral infection to prevent secondary homologous superinfection. Non-cytopathic bovine viral diarrhea virus (ncp BVDV) has been classified according to the exaltation of Newcastle disease phenomenon (END) as END positive (E+) and END negative (E-) st...

    journal_title:Acta virologica

    pub_type: 杂志文章

    doi:10.4149/av_2018_228

    authors: Muhsen M,Lee P

    更新日期:2018-01-01 00:00:00

  • Genotypic identification of seven Rickettsia conorii strains.

    abstract::Restriction endonuclease fragment length polymorphism (RFLP) analysis and polymerase chain reaction (PCR) were used to characterize the genotypic diversity of 7 strains of Rickettsia conorii from South Africa, Ethiopia, Morocco, India and Russia. The strains of R. conorii were divided into four genotypes by Rsa I or P...

    journal_title:Acta virologica

    pub_type: 杂志文章

    doi:

    authors: Zhang XF,Fan MY,Chen J,Bi DZ

    更新日期:1994-02-01 00:00:00

  • Coxsackievirus infection of mice. I. Viral kinetics and histopathological changes in mice experimentally infected with coxsackieviruses B3 and B4 by oral route.

    abstract::We followed the viral kinetics and histopathological changes in different organs of immunocompetent mice infected orally with coxsackieviruses B3 (CVB3) Nancy strain and B4 (CVB4) JVB strain separately. The viruses used were not adapted to mouse organs. In the acute phase of infection, the viral kinetics indicated vir...

    journal_title:Acta virologica

    pub_type: 杂志文章

    doi:

    authors: Bopegamage S,Borsanyiová M,Vargová A,Petrovicová A,Benkovicová M,Gomolcák P

    更新日期:2003-01-01 00:00:00

  • Identification of novel E347Q and G362K amino acid substitutions in HN neutralization epitope and major antigenic difference in novel sub-genotype VIIj isolates.

    abstract::Hemagglutinin-neuraminidase (HN) protein of Newcastle disease virus (NDV) plays a crucial role in induction of immune response against Newcastle disease infection. Mutation in residues 347 and 362 of HN linear antigenic site has been identified to be responsible for antigenic variations. Hence we studied antigenic dif...

    journal_title:Acta virologica

    pub_type: 杂志文章

    doi:10.4149/av_2017_405

    authors: Mayahi V,Esmaelizad M

    更新日期:2017-01-01 00:00:00

  • Effect of repeated Ribavirin treatment on grapevine viruses.

    abstract::The effect of Ribavirin treatment for the chemotherapy of several grapevine viruses was evaluated. Four grapevine cultivars were repeatedly treated with Ribavirin in two different concentrations and with three different lengths of treatment. Repeating the Ribavirin treatment always had a significant effect on the numb...

    journal_title:Acta virologica

    pub_type: 杂志文章

    doi:10.4149/av_2016_04_400

    authors: Komínek P,Komínková M,Jandová B

    更新日期:2016-01-01 00:00:00

  • Coxiella burnetii phase I and II proteins studied by SDS-page.

    abstract::Coxiella burnetii cells in both phase I and II reveal in sodium dodecylsulphate-polyacrylamide gel electrophoresis (SDS-PAGE) similar protein profiles with only small differences. C. burnetii protein profile in SDS-PAGE depended on the method of purification of C. burnetii cells from chick embryo yolk sacs. Immune mou...

    journal_title:Acta virologica

    pub_type: 杂志文章

    doi:

    authors: Lukácová M,Kazár J,Gajdosová E

    更新日期:1994-10-01 00:00:00

  • Activities of a lagging DNA strand synthesis of nucleoprotein complexes harboring an extrachromosomal DNA closely related to avian myeloblastosis virus core-bound DNA.

    abstract::Nucleoprotein (NP) complexes constituting the material of the postmicrosomal sediment (POMS) and its three basic components (A, B, C) (Ríman and Sulová, 1997a), harboring an extrachromosomal DNA closely related to AMV DNA were found to possess DNA- and RNA-synthesizing activities (SAs) reflecting the ability of this m...

    journal_title:Acta virologica

    pub_type: 杂志文章

    doi:

    authors: Ríman J,Sulová A

    更新日期:1997-08-01 00:00:00

  • Influence of the isolation procedure on the structure, neuraminidase and heamagglutinating activities of Sendai virus envelope glycoproteins.

    abstract::The influence of the isolation procedure of Sendai virus glycoproteins from the virus surface on their neuraminidase and haemagglutinating activities, as well as on some structural properties was studied. These glycoproteins exhibited lower specific neuraminidase and haemagglutinating activities than those of intact v...

    journal_title:Acta virologica

    pub_type: 杂志文章

    doi:

    authors: Popa LM,Repanovici R,Samuel I

    更新日期:1978-11-01 00:00:00

  • Recent measles viral activity in Uruguai: serological and genetic approaches.

    abstract::In the summer 1999, a measles outbreak occurred in Uruguai. During this outbreak 58 cases were recorded, 36 of which were laboratory confirmed as positive for measles virus (MV) IgM. The cases occurred in touristic places (Montevideo and Maldonado) predominantly among health facilities and tourist service personnel. U...

    journal_title:Acta virologica

    pub_type: 杂志文章

    doi:

    authors: Cánepa E,Siqueira MM,Hortal M,Friedrich F

    更新日期:2000-02-01 00:00:00