Coxiella burnetii phase I and II proteins studied by SDS-page.

Abstract:

:Coxiella burnetii cells in both phase I and II reveal in sodium dodecylsulphate-polyacrylamide gel electrophoresis (SDS-PAGE) similar protein profiles with only small differences. C. burnetii protein profile in SDS-PAGE depended on the method of purification of C. burnetii cells from chick embryo yolk sacs. Immune mouse sera against C. burnetii phase I cells recognized in phase I and II cell protein profiles mainly the 61 K and 29 K proteins by the immunoblot method. Hyperimmune mouse and rabbit sera against phase I and II cells reacted in different way with phase I and II cells. Sera against phase I cells recognized in both phase I and II profiles more protein bands than sera against phase II cells. Thus phase I LPS present in phase I cells exerted adjuvant effect on the antibody response in animals immunized with phase I cells.

journal_name

Acta Virol

journal_title

Acta virologica

authors

Lukácová M,Kazár J,Gajdosová E

subject

Has Abstract

pub_date

1994-10-01 00:00:00

pages

263-7

issue

5

eissn

0001-723X

issn

1336-2305

journal_volume

38

pub_type

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