Abstract：
:Solid-phase enzyme-immunoassay (EIA) used for the detection of hepatitis A virus (HAV) often reveals a non-specific activity which can be reduced or fully eliminated in the presence of normal serum. The factor responsible for this activity appeared to be a non-viral EIA-active material (NVEAM) that non-specifically reacted with normal serum of some mammalian species (human, monkey, rabbit, cattle). The HAV and NVEAM have been separated by CsC1 gradient centrifugation, where the HAV banded in a narrow zone at 1.34 g/cm3, whereas the NVEAM could be found in a wider zone with an average density of 1.31 g/cm3. Non-immunological character of the non-specific activity was demonstrated by the inhibitory effect of weak non-ionic detergents (0.05% Tween 20 or bile, 1:500). The conditions for preferential binding of the HAV by immune sera and elimination of non-specific reaction have been determined.
journal_name
Acta Viroljournal_title
Acta virologicaauthors
Tolskaya EA,Savinskaya SS,Balayan MS,Andzhaparidze AG,Kolesnikova MSsubject
Has Abstractpub_date
1983-01-01 00:00:00pages
43-50issue
1eissn
0001-723Xissn
1336-2305journal_volume
27pub_type
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