Abstract:
:A 189-bp repeat has been reported previously to be present in the replication origin (ORI) of Human cytomegalovirus (HCMV) Towne strain and to contain essential and supporting sequences (Chen et al., Arch. Virol. 141, 13301 (1996)). In this study, clones of HCMV strain Towne were isolated and analyzed for the copy number of the 189-bp repeat within its ORI (oriLyt). Southern blot analysis revealed that out of 14 clones, two contained a dimer, two contained a tetramer, and the remaining contained a trimer of the 189-bp repeat. Nucleotide sequence analysis disclosed that, within the dimer and the tetramer, the 189-bp repeat was present in a directly repeated manner. In this way we demonstrated an HCMV intrastrain variation in the copy number of the 189-bp repeat. Dot blot hybridization and growth curve analysis showed that the clones containing the dimer or the tetramer replicated in infected cells with similar efficiencies. We conclude that the HCMV oriLyt with spatial separation of the essential flanking regions by various copy number of the 189-bp repeat acts as ORI in the viral genome.
journal_name
Acta Viroljournal_title
Acta virologicaauthors
Chen Z,Watanabe Ssubject
Has Abstractpub_date
2007-01-01 00:00:00pages
21-6issue
1eissn
0001-723Xissn
1336-2305journal_volume
51pub_type
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