Abstract：
:Structural proteins V1, V2 and V3 of Japanese encephalitis virus (JEV) were isolated and purified by means of polyacrylamide gel electrophoresis (PAGE) in the presence of sodium dodecyl sulfate (SDS) and extracted from the sliced gel. The purified and renatured V3 envelope protein was found to bind to receptors for JEV on red blood cells. V3 also bound to haemagglutination inhibition (HI) antibodies from anti-JEV sera. In addition, V3 protein induced neutralizing antibodies against JEV when injected into mice. These facts strongly suggest that V3 protein carries the antigenic epitope(s) that elicit neutralizing antibodies and react with HI antibodies. We conclude the V3 plays an important role in virus infection. V2 protein did not exhibit these biological activities, but antibody against V1 protein, not contaminated with V3 protein, showed slight virus neutralizing activity. Possible implications of these results are discussed.
journal_name
Acta Viroljournal_title
Acta virologicaauthors
Takegami T,Miyamoto H,Nakamura H,Yasui Ksubject
Has Abstractpub_date
1982-09-01 00:00:00pages
312-20issue
5eissn
0001-723Xissn
1336-2305journal_volume
26pub_type
杂志文章相关文献
ACTA VIROLOGICA文献大全abstract：:This study comprised 100 persons with antibodies to hepatitis C virus (HCV), including 77 intravenous drug users (IVDUs). They were tested with serological HCV typing assays (Murex HCV serotyping 1-6 assay; Chiron RIBA HCV Serotyping SIA). Patients with a positive polymerase chain reaction (PCR) for HCV (n = 66) were ...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1997-10-01 00:00:00
abstract：:Two monoclonal antibodies (MoAb) to the Skalica virus from the tick-borne encephalitis (TBE) complex were used to compare Karshi and Royal Farm viruses with the Russian spring-summer encephalitis, Central European encephalitis (Hypr and Kumlinge strains) Skalica, Langat and Powassan viruses. The first MoAb was prepare...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1990-02-01 00:00:00
abstract：:A total of 22 persons, given a single intramuscular dose of 3.1 times 10(6) newborn mouse ic LD50/ml of the E5"14" virus (stored for 26 months at 4 degrees C in lyophilized state), were subjected to rigorous clinical, clinical-laboratory and virological investigations. Clinical observations and laboratory tests (blood...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1975-04-01 00:00:00
abstract：:Different concentrations of chemically-treated (by potassium periodate oxidation or mild acid hydrolysis) purified phase I Coxiella burnetii (C.b.) corpuscles and natural (untreated) purified phase I and phase II C.b. corpuscles were compared by ELISA for detection of both phase I (directed to antigen 1) and phase II ...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1987-05-01 00:00:00
abstract：:We followed the viral kinetics and histopathological changes in different organs of immunocompetent mice infected orally with coxsackieviruses B3 (CVB3) Nancy strain and B4 (CVB4) JVB strain separately. The viruses used were not adapted to mouse organs. In the acute phase of infection, the viral kinetics indicated vir...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：2003-01-01 00:00:00
abstract：UNLABELLED:Influenza A (H1N1) virus is an important pathogen that can be transmitted in the enzootic cycle involved in influenza epidemics. In this study, we analyzed natural selection on the 260 sequences of subtype 1 hemagglutinin (HA1) gene of H1N1 viruses isolated from humans and swine in Asia. Nonsynonymous and sy...
journal_title：Acta virologica
pub_type： 杂志文章
doi：10.4149/av_2010_02_113
更新日期：2010-01-01 00:00:00
abstract：:The lytic activity of splenocytes from C3H mice immunized with a highly attenuated line of Langat virus [tick-borne encephalitis (TBE) complex] was determined by the in vitro 51Cr release assay on TBE virus (western subtype)-infected target L929 cells. After the spleen cell suspensions were depleted of T cells with an...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1981-01-01 00:00:00
abstract：:Five monoclonal antibodies (MoAbs) against Indian reference/vaccine strain of foot-and-mouth disease (FMD) virus subtype A22 (IND17/77) and a guinea pig antibody against a synthetic peptide representing amino acids (aa) 136-151 of VP1 polypeptide of A22 virus were used in the study. All the antibodies either failed to...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1997-06-01 00:00:00
abstract：:Chikungunya (CHIK) virus is prevalent throughout Southeast Asia and Africa. It has caused numerous large outbreaks in India. No active or passive surveillance has been carried out since the last epidemic occurring in 1971. During a recent outbreak of Dengue (DEN)-like illness in eastern India, Aedes aegypti mosquitoes...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：2001-01-01 00:00:00
abstract：:A lipophilic thermostable lipopolysaccharide (LPS) complex was isolated by phenol extraction from purified suspensions of the typhus group rickettsiae. The LPS complex is antigenic and possesses some endotoxic properties such as toxicity for actinomycin D-treated mice, pyrogenicity for rabbits and guinea pigs, ability...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1977-09-01 00:00:00
abstract：:Chromosomal DNAs isolated from eight individuals from the Slovak population and from lymphoblastoid Namalwa cells were analyzed for the presence of genes coding for three subvariants of human interferon-alpha 2 (IFN-alpha 2), namely a, b, and c. The respective genes are regarded allelic, because they differ in the cod...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1994-04-01 00:00:00
abstract：:Haemocytes of laboratory bred half-engorged Hyalomma dromedarii ticks phagocytized intracoelomally inoculated Coxiella burnetii organisms. Significantly higher phagocytosis of phase II than phase I C. burnetii was observed, irrespective of whether live, killed untreated or killed organisms treated with chloroform-meth...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1984-01-01 00:00:00
abstract：:Antibodies against (a) naked particles, (b) virus envelope, and (c) whole herpes simplex virus (HSV) type 1 were investigated. Immune rabbit serum against naked particles (and formalized naked particles) contained virus neutralizing (VN) antibody in a low titre as compared to the titre of complement-fixing (CF) antibo...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1976-02-01 00:00:00
abstract：:Monoparacoumarylputrescinium chloride (pCPH), supplied to detached leaves of Gomphrena globosa via the petiole, induced interference with tomato bushy stunt virus infection by reducing the number and size of the necrotic local lesions. The phenolic compound neither inactivated directly the virus in vitro, nor induced ...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1981-03-01 00:00:00
abstract：:Clover blotch virus (CBV) was purified by differential centrifugation or precipitation with polyethylene glycol 6000 or 20000. Analytical centrifugation of purified CBV preparations revealed 2 components, the proportions of the lighter (96 S) nad heavier (112 S) being 88 and 12%, respectively. RNA isolated from purifi...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1975-09-01 00:00:00
abstract：:Most adults are asymptomatically infected with Epstein-Barr virus (EBV). Primary EBV infection is commonly associated with acute infectious mononucleosis (IM). T cell immune activation plays an important role in EBV-associated diseases. IM shows a mainly Th1-type profile, so Th1-type cytokines such as interleukin-2 (I...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：2004-01-01 00:00:00
abstract：:Eperythrozoon was detected in suckling mice inoculated with a suspension of nymphal Ixodes ricinus ticks. The organism was filterable through 220 nm Millipore membranes, moderately sensitive to diethyl ether, pathogenic to suckling but not to adult mice when given intracerebrally, intraperitoneally or subcutaneously, ...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1993-12-01 00:00:00
abstract：:A noninfectious soluble antigen fraction of goat poxvirus (GPV) fractionated by ammonium sulfate precipitation was tested for its suitability as coating antigen in an indirect enzyme-linked immunosorbent assay (ELISA). Accordingly, an avidin-biotin ELISA for the detection of GPV antibodies was optimized and evaluated ...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1999-10-01 00:00:00
abstract：:A method for the preparation of stable suspensions of erythrocytes sensitized with herpes simplex virus (HSV) antigen and for their use in the passive haemagglutination test (PHAT) was developed. Formolized sheep erythrocytes were treated with tannin and sensitized with HSV antigen prepared from infected chick embryo ...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1976-10-01 00:00:00
abstract：:Restriction endonuclease fragment length polymorphism (RFLP) analysis and polymerase chain reaction (PCR) were used to characterize the genotypic diversity of 7 strains of Rickettsia conorii from South Africa, Ethiopia, Morocco, India and Russia. The strains of R. conorii were divided into four genotypes by Rsa I or P...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1994-02-01 00:00:00
abstract：:S100P belongs to several members of the S100 family of calcium-binding proteins, associated with malignant phenotype. Altered levels of S100P expression have been described at different stages and types of cancer. Transcriptional regulation involves different pathways activated by glucocorticoids, growth factors and b...
journal_title：Acta virologica
pub_type： 杂志文章,评审
doi：10.4149/av_2013_02_238
更新日期：2013-01-01 00:00:00
abstract：:It has been shown that serum blocking factor(s) (SBF) previously detected in normal mice bind(s) to receptors on the surface membranes of virus-induced autoreactive T lymphocytes (ARTL) and to that of ARTL activity inhibiting T suppressors (TSar). The interaction of SBF with the receptors is reversible, H-2 restricted...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1985-01-01 00:00:00
abstract：:Human rotavirus strain 649 was isolated from a child with clinical picture of acute intestinal infection in the West European part of the U.S.S.R. This strain was adapted to MA-104 (rhesus monkey kidney) cells. By electrophoresis and haemagglutination it differed from the human rotavirus strain Wa and from the simian ...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1991-01-01 00:00:00
abstract：:Immobilization stress (hypokinesis) in Balb/c mice may aggravate asymptomatic infection with Langat virus (strain TP-21) as evidenced by 4-fold increased lethality in comparison with control animals. The virus levels in the spleen and brain of stressed and infected mice and the in vitro yield of the virus in immunocom...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1990-05-01 00:00:00
abstract：:Protective effects of different types of Q fever vaccines, namely untreated Coxiella burnetii phase I cells (Cb I) or Cb I cells treated with chloroform-methanol (CM) mixture (Cb I-CM) and of a Q fever chemovaccine obtained by trichloroacetic acid extraction (TCAE) from intact Cb I cells, were compared in mice and gui...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1986-11-01 00:00:00
abstract：:Besides other factors, occurrence of plum pox virus (PPV)-caused spots and mosaic symptoms on leaves of stone fruits are known to influence important physiological functions including production of assimilates. Apricot (Prunus armeniaca L.) seedlings were used as a test material for typical manifestation of symptoms o...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1998-09-01 00:00:00
abstract：:Hemagglutinin-neuraminidase (HN) protein of Newcastle disease virus (NDV) plays a crucial role in induction of immune response against Newcastle disease infection. Mutation in residues 347 and 362 of HN linear antigenic site has been identified to be responsible for antigenic variations. Hence we studied antigenic dif...
journal_title：Acta virologica
pub_type： 杂志文章
doi：10.4149/av_2017_405
更新日期：2017-01-01 00:00:00
abstract：:A decrease in creatine phosphokinase (CPK) activity was observed in plasma membranes (PM) treated with native A/WSN influenza virus but not in PM treated with heat-inactivated virus. The decrease in CPK activity depended on the amount of virus added to PM, on the pH of the medium and on the quality of the isolated PM....
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1975-04-01 00:00:00
abstract：:One-step real-time RT-PCR assay was developed for quantification of the immediate-early (IE), namely IE1 and IE2 transcripts of Rat cytomegalovirus (RCMV), strain ALL-03 in rat embryonic fibroblast cells (REF). This in-house SYBR Green I based RT-PCR was shown to have higher amplification efficiency and detection limi...
journal_title：Acta virologica
pub_type： 杂志文章
doi：10.4149/av_2009_04_261
更新日期：2009-01-01 00:00:00
abstract：:While the main interest in the pathogenesis of herpes simplex virus (HSV) in the sixties had been focussed on acute infections, in the seventies latent infection has become the main foal of investigation. Despite of overwhelming literature, the HSV persistence has remained a continuing problem from the practical as we...
journal_title：Acta virologica
pub_type： 杂志文章,评审
doi：
更新日期：1983-09-01 00:00:00
