Abstract：
UNLABELLED:Influenza A (H1N1) virus is an important pathogen that can be transmitted in the enzootic cycle involved in influenza epidemics. In this study, we analyzed natural selection on the 260 sequences of subtype 1 hemagglutinin (HA1) gene of H1N1 viruses isolated from humans and swine in Asia. Nonsynonymous and synonymous substitution rates (dN, dS) were determined for each amino acid site in the HA1 coding region. Four and two positively selected sites were identified in human and swine viruses, respectively. These sites might be involved in the change of antigenic epitopes to evade the host immune system. The results indicate that more diversifying and less purifying selection controls HA1 of human viruses compared with the swine viruses. Furthermore, thirteen sites evolving differently in human and swine viruses were identified in the HA1. These sites may play a role in the cross-species transmission. The analysis of adaptive evolution of the HA1 can provide valuable information for accelerating vaccine and drug development for prevention and treatment of influenza A (H1N1) in Asia. KEYWORDS:Influenza A virus; H1N1; hemagglutinin; positive selection; antigenic epitope.
journal_name
Acta Viroljournal_title
Acta virologicaauthors
Xu Z,Zhou R,Jin M,Chen Hdoi
10.4149/av_2010_02_113subject
Has Abstractpub_date
2010-01-01 00:00:00pages
113-8issue
2eissn
0001-723Xissn
1336-2305journal_volume
54pub_type
杂志文章相关文献
ACTA VIROLOGICA文献大全abstract：:Hemagglutinin-neuraminidase (HN) protein of Newcastle disease virus (NDV) plays a crucial role in induction of immune response against Newcastle disease infection. Mutation in residues 347 and 362 of HN linear antigenic site has been identified to be responsible for antigenic variations. Hence we studied antigenic dif...
journal_title：Acta virologica
pub_type： 杂志文章
doi：10.4149/av_2017_405
更新日期：2017-01-01 00:00:00
abstract：:The influenza virus M2 protein has an ion channel activity that permits ions to enter the virion during its uncoating and also modulates pH of intracellular compartments. M2 protein is a homotetramer consisting of either a pair of disulfide-linked dimers or a disulfide-linked tetramer. The M2 trans-membrane domain pep...
journal_title：Acta virologica
pub_type： 杂志文章,评审
doi：
更新日期：1995-06-01 00:00:00
abstract：:Betaflexiviridae is a family of plant-infecting RNA viruses with 11 recognized genera, of which genomes have diverse organization with three to six open reading frames (ORFs). A genome sequence of a novel Betaflexiviridae species, named Salvia divinorum RNA virus 1 (SdRV1), was identified in Salvia divinorum, herbal m...
journal_title：Acta virologica
pub_type： 杂志文章
doi：10.4149/av_2019_401
更新日期：2019-01-01 00:00:00
abstract：:Extreme antigenic and genetic heterogeneity of serotype A foot-and-mouth disease virus (FMDV) population has resulted in change of vaccine strains in India twice in the last decade. In such a situation, complete characterization of the vaccine strains is imperative. With regard to the frequent outbreaks of this diseas...
journal_title：Acta virologica
pub_type： 杂志文章
doi：10.4149/av_2011_04_349
更新日期：2011-01-01 00:00:00
abstract：:Sera of the influenza patients and healthy controls were tested for some types of autoantibody (SMA, ANA, ABBA, AMA). They were detected in 83.8% of the patients' sera and in 16.6% of controls. SMA were present in 77.4%, ANA in 54.8%, and ABBA in 16.1% of the patient's sera. AMA were not detected. A majority of the se...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1976-06-01 00:00:00
abstract：:In this study, DNA vaccines consisting of vector IRES-gD expressing Herpes simplex virus 1 (HSV-1) glycoprotein D (gD) and vector IRES-gD-IL-2 coexpressing HSV-1 gD and interleukin-2 (IL-2), respectively, were constructed. After intramuscular inoculation, both vaccines induced in BALB/c mice antibodies as assayed by E...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：2006-01-01 00:00:00
abstract：:Tick-borne viruses are causative agents of several important human diseases. Tick-borne encephalitis virus (TBEV) is the most prominent representative considered medically to be the most important arbovirus (arthropod-borne virus) in Europe and northern Asia. Tick-borne virus transmission cycles are determined by the ...
journal_title：Acta virologica
pub_type： 杂志文章,评审
doi：10.4149/av_2013_02_123
更新日期：2013-01-01 00:00:00
abstract：:In September 2011, the leaf samples of hosta cultivar 'Sum and substance' were collected from the collection of Gryshko' National Botanical Garden in Kyiv. The leaves showed dark green streaking and puckering along the leaf veins. Transmission electron microscopy revealed the presence of filamentous viral particles 13...
journal_title：Acta virologica
pub_type： 杂志文章
doi：10.4149/av_2017_415
更新日期：2017-01-01 00:00:00
abstract：:Monocytes/macrophages have been known to play an important role in the initiation and propagation of human immunodeficiency virus 1 (HIV-1) infection. To analyze the function of these cells during the clinical asymptomatic period of infection, we examined the effect of murine peritoneal macrophages and human periphera...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1997-02-01 00:00:00
abstract：:Group A rotavirus infections were detected in 93 of 410 fecal samples from children with acute diarrhea, admitted in three main hospitals of Asunción, Paraguay, from August 1998 to August 2000. Most of the rotavirus-infected patients were admitted during the winter season in the three epidemic years. The rotavirus inf...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：2003-01-01 00:00:00
abstract：:Structural proteins V1, V2 and V3 of Japanese encephalitis virus (JEV) were isolated and purified by means of polyacrylamide gel electrophoresis (PAGE) in the presence of sodium dodecyl sulfate (SDS) and extracted from the sliced gel. The purified and renatured V3 envelope protein was found to bind to receptors for JE...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1982-09-01 00:00:00
abstract：:Analysis of cowpox outbreaks revealed an extremely wide range of virus pathogenicity including 9 orders of mammals. Recent serological and virological data support the hypothesis that wild rodents may be a natural reservoir of cowpox virus. Cowpox infection of humans occurring without any contact with infected cattle ...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1984-09-01 00:00:00
abstract：:RNA interference (RNAi) is a biological phenomenon in which introduction of a small, double-stranded interfering RNAs (siRNAs) into a cell causes a specific degradation of homologous single-stranded RNA. siRNA can be delivered into the cell by different approaches including synthetic RNA, in vitro transcribed RNA and ...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：2005-01-01 00:00:00
abstract：:Differences in interfering activities were demonstrated between low-passage epidemic and thermosensitive cold-adapted strains of influenza viruses A and B in experiments on chick embryos inoculated on the chorioallantoic membranes and on white mice. At a high multiplicity of infection the thermosensitive variants show...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1982-05-01 00:00:00
abstract：:RNA of partially purified Kemerovo virus sedimented in a relatively broad region in sucrose density gradient with a peak at 14 S. Approximately 65% of this RNA was resistant to the action of ribonuclease which indicates the double-stranded nature of Kemerovo virus RNA. ...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1975-01-01 00:00:00
abstract：:A rat glioma cell line, C6-BU-1, showed differential susceptibility to herpes simplex virus type 1 (HSV-1) and type 2 (HSV-2), namely, all the HSV-1 strains tested so far persisted in this cell line but the HSV-2 strains did not. Two clones were derived from C6-BU-1 cells and designated C-17 and C-72. The C-72 as well...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1991-04-01 00:00:00
abstract：:Eperythrozoon was detected in suckling mice inoculated with a suspension of nymphal Ixodes ricinus ticks. The organism was filterable through 220 nm Millipore membranes, moderately sensitive to diethyl ether, pathogenic to suckling but not to adult mice when given intracerebrally, intraperitoneally or subcutaneously, ...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1993-12-01 00:00:00
abstract：:Identification of structural protein relationships is likely to be important for virus assembly and anti-WSSV strategies. In this paper, VP90 of white spot syndrome virus (WSSV) was characterized. Temporal-transcription analysis showed that VP90 is expressed in the late stage of WSSV infection. Far-western and matrix-...
journal_title：Acta virologica
pub_type： 杂志文章
doi：10.4149/av_2012_01_57
更新日期：2012-01-01 00:00:00
abstract：:Although most pathogenic rickettsiae are obligate intracellular parasites, it is clear that they are bacteria. As such, form and function in rickettsiae are closely similar to form and function found in their free-living counterparts. This review of rickettsiae as bacteria portrays the broad similarities of rickettsia...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1985-09-01 00:00:00
abstract：:Solid-phase enzyme-immunoassay (EIA) used for the detection of hepatitis A virus (HAV) often reveals a non-specific activity which can be reduced or fully eliminated in the presence of normal serum. The factor responsible for this activity appeared to be a non-viral EIA-active material (NVEAM) that non-specifically re...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1983-01-01 00:00:00
abstract：:The H9N2 influenza virus has been frequently endemic in poultry, infected mammals and humans and has threatened public health. It is therefore imperative to understand the molecular mechanism enabling this virus to jump from avian to mammalian species. In this study, two H9N2 influenza viruses were isolated from the s...
journal_title：Acta virologica
pub_type： 杂志文章
doi：10.4149/av_2020_109
更新日期：2020-01-01 00:00:00
abstract：:Hepatitis C virus (HCV) infection represents a serious public health problem worldwide. Development of new therapeutics against HCV has been hampered by the lack of a small-animal model. Until now, it has been unclear which host factors influence the HCV infection. It was known that human CD81 (hCD81) or low-density l...
journal_title：Acta virologica
pub_type： 杂志文章
doi：10.4149/av_2009_03_185
更新日期：2009-01-01 00:00:00
abstract：:The coat proteins of both ordinary (PVSO) and Andean (PVSA) strains were shown to be similar in molecular weights, peptide maps, amino acid analysis and reaction to a range of carlavirus antiseras. ...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1992-03-01 00:00:00
abstract：:Group A rotavirus is the leading cause of acute gastroenteritis in cattle and swine. Although, vaccination against this virus is an effective strategy for prevention, additional strategy to control disease is necessary. Egg yolk immunoglobulin (IgY)-based passive immunization could be a better option in preventing thi...
journal_title：Acta virologica
pub_type： 杂志文章
doi：10.4149/av_2017_02_03
更新日期：2017-01-01 00:00:00
abstract：:We characterized leukocytes in peripheral blood of BALB/c mice infected with mouse herpesvirus isolate 72 (MHV-72) representing an isolate of mouse herpesvirus strain 68 (MHV-68, species Murid herpesvirus 4, genus Rhadinovirus, subfamily Gammaherpesvirinae, family Herpesviridae) (van Regenmortel et al., 2000). In acut...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：2002-01-01 00:00:00
abstract：:Viraemia was studied in adult Clethrionomys glareolus subcutaneously infected with 12 strains of tick-borne encephalitis (TBE) virus isolated in western and eastern foci of TBE. Nine strains caused viraemia regularly or irregularly, reaching titres higher than the threshold level of TBE virus infectivity for its vecto...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1979-05-01 00:00:00
abstract：:An indirect enzyme-linked immunosorbent assay (ELISA) was developed for the detection of antibodies against equine herpesvirus type 2 (EHV-2) in equine sera. The optimal conditions of antigen concentration, and serum and conjugate dilutions were established by chequerboard titrations. When the standard ELISA test was ...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1987-11-01 00:00:00
abstract：:Acute gastroenteritis (AGE) is one of the most frequently occuring illnesses in children and adults worldwide. In February 2017, two AGE outbreaks occurred in two adjacent schools in Huzhou city, Zhenjiang province of China. We detected high percentages of recombinant norovirus GII.P16/GII.2 in one school and chicken ...
journal_title：Acta virologica
pub_type： 杂志文章
doi：10.4149/av_2019_312
更新日期：2019-01-01 00:00:00
abstract：:Interferon (IFN) was induced in chicken peripheral blood leukocytes by exposure to Concanavalin A (Con A). It has reached a maximum level 96 hr after stimulation with the optimum dose of 10 micrograms/ml. The IFN was partially purified by chromatography on controlled-pore glass adsorbent and was characterized as a fai...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1986-03-01 00:00:00
abstract：:CVI988/Rispens strain of Marek's disease virus type I (MDV-1) is widely used as efficient vaccine to control Marek's disease (MD) in chicken flocks. Similarly to other live MD vaccine viruses it is propagated in freshly prepared chicken embryo fibroblasts (CEF). In this study, MDV-1 CVI988/Rispens strain was adapted t...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：2001-04-01 00:00:00
