Abstract:
:Electron microscopy showed that commerical poly I: C consisted of molecules varying in length from less than 0.05 nm to more than 5 nm and also in morphology . To clarify the relationship between its molecular size and interferon-inducing activity, poly I: C was sonicated or fractionated by sucrose density gradient centrifugation, and the molecular length distribution and interferon-inducing activity of each preparation was determined in vivo and in vitro. The results showed that (1) poly I : C molecules 0.1-0.3 nm long were the most effective for interferon induction; (2) sonication of poly I : C reduced its molecular length and also the interferon-inducing activity, the degree of reduction varying in different fractions; and (3) the interferon-inducing activity of poly I: C of 0.1-0.3 nm obtained by sucrose density gradient centrifugation was higher than that poly I: C of corresponding length prepared by sonication.
journal_name
Acta Viroljournal_title
Acta virologicaauthors
Arimura Hsubject
Has Abstractpub_date
1975-11-01 00:00:00pages
457-66issue
6eissn
0001-723Xissn
1336-2305journal_volume
19pub_type
杂志文章相关文献
ACTA VIROLOGICA文献大全abstract::Phages are excellent models for studying the mechanism of DNA replication in prokaryotes. Identification of phage proteins involved in phage DNA replication is the first prerequisite for elucidation of the phage replication module. We focused on replication proteins gp41 (a putative helicase from SF2 superfamily), gp4...
journal_title:Acta virologica
pub_type: 杂志文章
doi:10.4149/av_2014_02_152
更新日期:2014-01-01 00:00:00
abstract::Herpes simplex virus 1 (HSV-1) is one of the most prevalent human pathogens in both industrialized and developing countries. This study was performed to analyze the antiviral activity of purified flavonoid from Polygonum perfoliatum L. against HSV-1 infection in vitro and in vivo. Flavonoid showed no inhibitory effect...
journal_title:Acta virologica
pub_type: 杂志文章
doi:10.4149/av_2014_04_368
更新日期:2014-01-01 00:00:00
abstract::A peculiar grouping of Creutzfeldt-Jacob disease (CJD) and of other organic presenile dementia (OPD) cases in a small, prevalently rural area in Czechoslovakia to the east of 19 degrees 35' E and to the north of 48 degrees 15' N was studied. Between August, 1972 and November, 1976, three histologically proven CJD case...
journal_title:Acta virologica
pub_type: 杂志文章
doi:
更新日期:1978-03-01 00:00:00
abstract::With only a single class of antiviral drugs existing for treatment of influenza (neuraminidase inhibitors), the search for novel effective compounds is urgently needed. We evaluated a low molecular mass compound, enisamium iodide (FAV00A), against influenza virus infections in primary differentiated normal human bronc...
journal_title:Acta virologica
pub_type: 杂志文章
doi:10.4149/av_2018_211
更新日期:2018-01-01 00:00:00
abstract::Several putative cell surface receptors have been identified in the Hepatitis C virus (HCV) infection including CD81, low-density lipoprotein (LDL) receptor, scavenger receptor class B type I (SR-BI), and highly sulfated heparan sulfate (HS). In this study, we showed that the binding of soluble heparin to the envelope...
journal_title:Acta virologica
pub_type: 杂志文章
doi:
更新日期:2008-01-01 00:00:00
abstract::Four isolates of measles virus (Gag, Il, Buk and Shed) were obtained from suspensions of mononuclear cells from patients at the active stage of the disease. Vero cells were used for the virus isolation. All the isolates caused in the infected cell culture the appearance of symplasts of differently sized, star- or spin...
journal_title:Acta virologica
pub_type: 杂志文章
doi:
更新日期:1992-12-01 00:00:00
abstract::A highly specific rabbit antiserum raised against anionic peroxidase (PRX) purified from cucumber (Cucumis sativus L., cv. Laura) cotyledons infected with tobacco necrosis virus (TNV) was used to further investigate the antigenic relatedness of peroxidases in the selected plant species. Immunoblot analysis using nativ...
journal_title:Acta virologica
pub_type: 杂志文章
doi:
更新日期:1996-06-01 00:00:00
abstract::Five monoclonal antibodies (MoAbs) against Indian reference/vaccine strain of foot-and-mouth disease (FMD) virus subtype A22 (IND17/77) and a guinea pig antibody against a synthetic peptide representing amino acids (aa) 136-151 of VP1 polypeptide of A22 virus were used in the study. All the antibodies either failed to...
journal_title:Acta virologica
pub_type: 杂志文章
doi:
更新日期:1997-06-01 00:00:00
abstract::A nation-wide vaccination against mumps that had been launched in the Czech Republic in 1987 eliminated great outbreaks (up to 100,000 cases per year) of this disease in 1955-1988, but did not prevent small outbreaks (a few thousand cases per year) in 1995-1996, 2005-2007, and 2010-2012. The extent of these small outb...
journal_title:Acta virologica
pub_type: 杂志文章
doi:
更新日期:2013-01-01 00:00:00
abstract::Two Infectious bursal disease virus (IBDV) isolates, NP1SSH and NP2K were obtained from a severe infectious bursal disease (IBD) outbreak in Nepal in 2002. The hypervariable (HV) region of VP2 gene (1326 bp) of the isolates was generated by RT-PCR and sequenced. The obtained nucleotide sequences were compared with tho...
journal_title:Acta virologica
pub_type: 杂志文章
doi:
更新日期:2005-01-01 00:00:00
abstract::We isolated plaque-size variants of a South American strain of yellow fever virus, and compared their ability to infect orally and be transmitted by vector Aedes aegypti mosquitoes with that of the uncloned, parental virus. We analyzed the same clonal isolates in mouse virulence experiments. No significant differences...
journal_title:Acta virologica
pub_type: 杂志文章
doi:
更新日期:1988-05-01 00:00:00
abstract::Peripheral blood cells of avian myeloblastosis virus (AMV-(infected chickens were examined at various intervals post infection by immunofluorescence. AMV revertase was identified in pro- and myeloblasts; it was localized mainly in the perinuclear zone or throughout the cytoplasm. No revertase was found in erythrocytes...
journal_title:Acta virologica
pub_type: 杂志文章
doi:
更新日期:1976-08-01 00:00:00
abstract::The ability of human peripheral blood leukocytes to produce interferon in response to phage double-stranded (ds) RNA was studied. Under the conditions used, interferon was produced not only by lymphocytes but also by polymorphs and monocytes. These "pure" cultures showed no marked differences in the degree of interfer...
journal_title:Acta virologica
pub_type: 杂志文章
doi:
更新日期:1977-11-01 00:00:00
abstract:UNLABELLED:DNA vaccines containing the capsid precursor polypeptide P1 gene of foot-and-mouth disease virus (FMDV) alone or combined with the VP22 gene of bovine herpesvirus 1 (BVP22) as molecular adjuvant were constructed and used for immunization of BALB/c mice. The latter were challenged with FMDV and their humoral ...
journal_title:Acta virologica
pub_type: 杂志文章
doi:10.4149/av_2012_02_111
更新日期:2012-01-01 00:00:00
abstract::Genetic composition and biological properties of influenza virus recombinants A/PR/8/34 (H1N1) and A/Greifswald/6/74 (H3N2) were analysed. The haemagglutinin (HA) gene of the strain A/PR/8/34 was shown an important part of the gene complex determining the virulence for mice, the yield of HA and the plaque forming capa...
journal_title:Acta virologica
pub_type: 杂志文章
doi:
更新日期:1983-11-01 00:00:00
abstract::According to the morphology, type and function of its structural components the segmented genome of type C influenza virus is similar to that of influenza viruses A and B. However, type C influenza virus differs from them by the reticular structure of virion surface, absence of the surface glycoprotein neuraminidase, ...
journal_title:Acta virologica
pub_type: 杂志文章
doi:
更新日期:1984-07-01 00:00:00
abstract::A strain designated 42-Edmonston was isolated from the brain of a Syrian hamster 30 days after inoculation during the neonatal period with the reference "wild" measles virus strain Edmonston which produced a latent infection accompanied by production of measles antibody and insignificant pathomorphological changes. In...
journal_title:Acta virologica
pub_type: 杂志文章
doi:
更新日期:1977-05-01 00:00:00
abstract::Chromic chloride and tannic acid methods were elaborated to bind purified adenovirus hexons to sheep red blood cells (SRBC). Either method gave very sensitive haemagglutination (HA) with adenovirus antisera, but treatment with tannic acid was more sensitive. Using this method, specific antibodies present in polyclonal...
journal_title:Acta virologica
pub_type: 杂志文章
doi:
更新日期:1985-09-01 00:00:00
abstract::An indirect immunofluorescence assay (IFA) using a recently developed commercial kit for detecting antibodies against Coxiella burnetii (C.b.), the etiological agent of Q fever, has been evaluated using human field serum samples. The IFA was compared with an ELISA and a complement fixation test (CFT). The IFA was base...
journal_title:Acta virologica
pub_type: 杂志文章
doi:
更新日期:2005-01-01 00:00:00
abstract::Cytotoxic T lymphocyte (CTL) activity of draining lymph node (DLN) cells isolated from BALB/c mice infected with ectromelia virus (EV) was examined using a fluorometric cell-mediated cytotoxicity (CMC) assay. Specific lysis of target cells A20 and EMT-6 primed with EV was demonstrated. The classical CD8+ cytolytic pat...
journal_title:Acta virologica
pub_type: 杂志文章
doi:
更新日期:1996-11-01 00:00:00
abstract::The discovery of respiratory tract involvement in the pathogenesis of foot-and-mouth disease (FMD) and the recognition of the role of inhalated aerosols in virus transfer opened new outlooks in the epizootology and prophylaxis of the disease. Nevertheless, FMD should be regarded for an infection transmitted by contami...
journal_title:Acta virologica
pub_type: 杂志文章,评审
doi:
更新日期:1983-07-01 00:00:00
abstract::Coxiella burnetii cells in both phase I and II reveal in sodium dodecylsulphate-polyacrylamide gel electrophoresis (SDS-PAGE) similar protein profiles with only small differences. C. burnetii protein profile in SDS-PAGE depended on the method of purification of C. burnetii cells from chick embryo yolk sacs. Immune mou...
journal_title:Acta virologica
pub_type: 杂志文章
doi:
更新日期:1994-10-01 00:00:00
abstract::After passaging the NWS influenza virus at increasing temperatures in the hamster embryo fibroblast (HEF) cell line, a temperature-resistant (tr) mutant which grew well at 39 degrees C was isolated. Attempts were made to isolate temperature-sensitive (ts) mutants from the tr virus. After growth in the presence of 5-fl...
journal_title:Acta virologica
pub_type: 杂志文章
doi:
更新日期:1976-08-01 00:00:00
abstract::In the summer 1999, a measles outbreak occurred in Uruguai. During this outbreak 58 cases were recorded, 36 of which were laboratory confirmed as positive for measles virus (MV) IgM. The cases occurred in touristic places (Montevideo and Maldonado) predominantly among health facilities and tourist service personnel. U...
journal_title:Acta virologica
pub_type: 杂志文章
doi:
更新日期:2000-02-01 00:00:00
abstract::Conditions of the efficient production of tick-borne encephalitis (TBE) virus in chick embryo cell (CEC) monolayer cultures were investigated. The prerequisites of high recovery of the produced virus appeared to be: the use of dense cell monolayers, reasonable frequency of harvests and a suitable buffering of nutrient...
journal_title:Acta virologica
pub_type: 杂志文章
doi:
更新日期:1983-03-01 00:00:00
abstract::Porcine epidemic diarrhea (PED) caused by virulent strains of porcine epidemic diarrhea virus (PEDV) is a highly contagious enteric disease of swine characterized by severe enteritis, vomiting, and watery diarrhea. This study investigates the subcellular localization and function of PEDV M protein through examination ...
journal_title:Acta virologica
pub_type: 杂志文章
doi:10.4149/av_2015_03_265
更新日期:2015-09-01 00:00:00
abstract::The lytic activity of splenocytes from C3H mice immunized with a highly attenuated line of Langat virus [tick-borne encephalitis (TBE) complex] was determined by the in vitro 51Cr release assay on TBE virus (western subtype)-infected target L929 cells. After the spleen cell suspensions were depleted of T cells with an...
journal_title:Acta virologica
pub_type: 杂志文章
doi:
更新日期:1981-01-01 00:00:00
abstract::The baculovirus-insect cell expression system (BES), one of the most popular systems for expression of eukaryotic proteins, was known to have drawbacks such as laborious manipulation of large-size baculovirus bacmids and the transfection procedure. These problems could be eliminated by direct infection of eukaryotic c...
journal_title:Acta virologica
pub_type: 杂志文章
doi:10.4149/av_2014_01_61
更新日期:2014-01-01 00:00:00
abstract::Every year, the poultry industry experiences significant economic losses due to epidemics of Newcastle disease virus (NDV). Developing new vaccines by identifying and using the immunogenic hemagglutinin-neuraminidase (HN) protein can protect the poultry industry. In the present study, the full-length HN protein was ex...
journal_title:Acta virologica
pub_type: 杂志文章
doi:10.4149/av_2020_310
更新日期:2020-01-01 00:00:00
abstract::Nonlethal infection was induced in laboratory mice with sublethal doses of rabies strains isolated from small wild rodents. Three types of nonlethal infecion were observed: persistent--with virus irregularly reisolable for at least 56 days after inoculation: latent--with negative virus reisolation but presence of infe...
journal_title:Acta virologica
pub_type: 杂志文章
doi:
更新日期:1980-09-01 00:00:00