Use of Tomato leaf curl virus (TYLCV) truncated Rep gene sequence to engineer TYLCV resistance in tomato plants.

Abstract:

:Tomato yellow leaf curl disease causes severe losses in tomato production throughout Mediterranean countries including Tunisia. In order to generate engineered resistance to this disease, an intron-hairpin RNA construct harboring a Tomato yellow leaf curl Sardinia virus (TYLCSV) truncated replication-associated protein (Rep) gene was used to transform genotype of tomato plants. Prepared transgenic plants were agro-inoculated with Tunisian infectious strain of TYLCSV and screened for the resistance to infection. The infected transgenic plants were divided into 3 different groups according to their specific symptoms. Only one of them contained transgenic plants fully resistant to the tomato yellow leaf curl disease.

journal_name

Acta Virol

journal_title

Acta virologica

authors

Ben Tamarzizt H,Gharsallah Chouchane S,Lengliz R,Maxwell DP,Marrakchi M,Fakhfakh H,Gorsane F

doi

10.4149/av_2009_02_99

subject

Has Abstract

pub_date

2009-01-01 00:00:00

pages

99-104

issue

2

eissn

0001-723X

issn

1336-2305

journal_volume

53

pub_type

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