Investigation of the relationship between Epstein-Barr virus and ordinary gastric carcinoma using the nested polymerase chain reaction.

Abstract:

:To investigate whether Epstein-Barr virus (EBV) is associated with ordinary gastric carcinoma and to determine its genotype, samples from ordinary gastric carcinoma from 65 patients (40 males, 20 females) and 21 endoscopic biopsies from 7 individuals with non-neoplastic mucosa were analysed using one-stage and nested (two-stage) polymerase chain reaction (PCR) assays. The nested PCR assay yielded 56.9% (37/65) and 52.3% (11/21) positivity for the ordinary gastric carcinoma and control cases, respectively; these results were significantly than those of the one-stage PCR assay (13.5% and 0%, respectively). The EBV positivity showed similar rate in male and female patients (60% versus 52%, P > 0.05). The dominant genotype of EBV was 1 (92%) followed by mixture of 1 + 2 (5.4%) and 2 (2.6%). In conclusion, similar positivity rates of EBV in neoplastic and non-neoplastic tissues suggest that the relationship of this virus to the ordinary gastric carcinoma is not clear.

journal_name

Acta Virol

journal_title

Acta virologica

authors

Durmaz R,Aydin A,Köroglu M,Durmaz B,Ciralik H

subject

Has Abstract

pub_date

1998-12-01 00:00:00

pages

359-63

issue

6

eissn

0001-723X

issn

1336-2305

journal_volume

42

pub_type

杂志文章
  • Transmission of alfalfa mosaic virus through Nicandra physaloides seeds and its localization in embryo cotyledons.

    abstract::Alfalfa mosaic virus (AMV) was found to be transmitted through seeds of Nicandra physaloids L. The average seed transmission rate of the AMV isolates T6, LMBG-4 and ST amounted to 23, 4 and 0 per cent, repectively. In the cytoplasm of parenchyma cells of embryo cotyledons of seeds from plants infected with the T6 isol...

    journal_title:Acta virologica

    pub_type: 杂志文章

    doi:

    authors: Gallo J,Ciampor F

    更新日期:1977-07-01 00:00:00

  • Inhibition of expression of rna polymerase with small interfering RNAs targeting a conserved motif in the respective viral genes in viruses of the family Flaviviridae.

    abstract::The RNA-dependent RNA polymerase (RdRp) in viruses of the family Flaviviridae plays an important role in the viral replication process and in the forming of a replicase complex. We used small interfering RNAs (siRNAs) corresponding to the highly conservative Motif V of RdRp gene of different viruses to examine their r...

    journal_title:Acta virologica

    pub_type: 杂志文章

    doi:

    authors: Xu Z,Li X,Liu R,Si Y,Sun M,Jin M,Chen H,Qian P

    更新日期:2007-01-01 00:00:00

  • E-rosette forming cells and humoral antibody titres in humans after vaccination with three different inactivated influenza virus vaccines A/USSR/92/77 (H1N1).

    abstract::The number of E-rosette forming cells and the serum haemagglutination inhibition (HI) antibody titres were examined in 37 volunteers immediately before and 14, 28, 35 and 63 days after immunization with three inactivated influenza virus vaccines A/USSR/92/77 (H1N1)--NIB 6 and in 11 non-vaccinated controls. From the fo...

    journal_title:Acta virologica

    pub_type: 杂志文章

    doi:

    authors: Schmidt S,Süss J,Oehring H,Schmidt J

    更新日期:1982-12-01 00:00:00

  • Evaluation of avidin-biotin ELISA for the detection of antibodies to goat poxvirus using noninfectious diagnostic reagent.

    abstract::A noninfectious soluble antigen fraction of goat poxvirus (GPV) fractionated by ammonium sulfate precipitation was tested for its suitability as coating antigen in an indirect enzyme-linked immunosorbent assay (ELISA). Accordingly, an avidin-biotin ELISA for the detection of GPV antibodies was optimized and evaluated ...

    journal_title:Acta virologica

    pub_type: 杂志文章

    doi:

    authors: Rao TV,Malik P,Asgola D

    更新日期:1999-10-01 00:00:00

  • Genetic characterization of wild-type measles viruses isolated during the 1998 measles epidemic in Argentina.

    abstract::This study reports on genomic characterization of six measles virus (MV) isolates obtained from a measles epidemic in Argentina in 1998. Reverse transcription-polymerase chain reaction (RT-PCR) and nucleotide sequence analysis of the carboxyl-terminal region of the nucleoprotein (N) gene of these isolates classified a...

    journal_title:Acta virologica

    pub_type: 杂志文章

    doi:

    authors: Baumeister E,Siqueira MM,Savy V,Friedrich F

    更新日期:2000-06-01 00:00:00

  • Role of genes 4 and 6 for the expression of some biological properties of influenza virus A/PR/8/34.

    abstract::Genetic composition and biological properties of influenza virus recombinants A/PR/8/34 (H1N1) and A/Greifswald/6/74 (H3N2) were analysed. The haemagglutinin (HA) gene of the strain A/PR/8/34 was shown an important part of the gene complex determining the virulence for mice, the yield of HA and the plaque forming capa...

    journal_title:Acta virologica

    pub_type: 杂志文章

    doi:

    authors: Döhner L,Herrmann B,Mentel R,Krenz S

    更新日期:1983-11-01 00:00:00

  • High Th1-type cytokine serum levels in patients with infectious mononucleosis.

    abstract::Most adults are asymptomatically infected with Epstein-Barr virus (EBV). Primary EBV infection is commonly associated with acute infectious mononucleosis (IM). T cell immune activation plays an important role in EBV-associated diseases. IM shows a mainly Th1-type profile, so Th1-type cytokines such as interleukin-2 (I...

    journal_title:Acta virologica

    pub_type: 杂志文章

    doi:

    authors: Corsi MM,Ruscica M,Passoni D,Scarmozzino MG,Gulletta E

    更新日期:2004-01-01 00:00:00

  • Localization of human recombinant adenoviral vectors to the mitochondria following transduction of human cell lines.

    abstract::Recently, mitochondria have been shown to have a vital role in the innate immune response to viral infection. In response, viruses such as adenovirus, have developed mechanisms to alter mitochondrial function through direct or indirect interaction with the mitochondria. The interaction of human recombinant adenoviral ...

    journal_title:Acta virologica

    pub_type: 杂志文章

    doi:10.4149/av_2019_114

    authors: Morrison BJ,Abu-Asab M,Morris JC,Steel JC

    更新日期:2019-01-01 00:00:00

  • Immunoblot analysis of antibody response in mice infected with Coxiella burnetii phase I.

    abstract::Mouse sera collected from day 4 to day 133 postinfection (p.i.) with phase I Coxiella burnetii strain Nine Mile were analysed by immunoblotting with phase I C. burnetii cell lysate. Antibodies of IgG class protein antigens were revealed already on day 10 p.i. (60, 49 and 27 kD proteins), followed by those to 77 kD pro...

    journal_title:Acta virologica

    pub_type: 杂志文章

    doi:

    authors: Novák M,Brezina R,Kazár J

    更新日期:1992-01-01 00:00:00

  • Eperythrozoon--a lesson for arbovirologists.

    abstract::Eperythrozoon was detected in suckling mice inoculated with a suspension of nymphal Ixodes ricinus ticks. The organism was filterable through 220 nm Millipore membranes, moderately sensitive to diethyl ether, pathogenic to suckling but not to adult mice when given intracerebrally, intraperitoneally or subcutaneously, ...

    journal_title:Acta virologica

    pub_type: 杂志文章

    doi:

    authors: Hubálek Z,Juricová Z,Halouzka J

    更新日期:1993-12-01 00:00:00

  • A Fe(3+)/DNA complex induces an anti-human immunodeficiency virus factor(s) in CD4+ lymphocyte cell lines.

    abstract::Numerous cytokines and chemokines are involved in inflammatory and immune response. Whereas some of them inhibit virus replication in vitro directly or increase the patients' T4-lymphocyte level, others effects are not so clear. Using human immunodeficiency virus (HIV) and cell cultures we have studied the antiviral e...

    journal_title:Acta virologica

    pub_type: 杂志文章

    doi:

    authors: Nossik D,Kaplina E,Nossik N,Kalnina L,Tsutsumi R,Miura Y,Sera K,Itoh C,Sato S,Lvov D

    更新日期:1999-02-01 00:00:00

  • The presence of interferon and type A immunoglobulins in the nasopharyngeal secretions of volunteers immunized with an inactivated influenza vaccine.

    abstract::The presence of interferon and type A immunoglobulins (IgA) was followed up in the nasopharyngeal washings collected from volunteers immunized intranasally with an inactivated influenza vaccine [strain A/Rom 1/73 (H3N2)]. Interferon was detected 24 hours after vaccine administration, its incidence being similar to tha...

    journal_title:Acta virologica

    pub_type: 杂志文章

    doi:

    authors: Danielescu G,Barbu C,Sorodoc Y,Cajal N,Sărăteanu D

    更新日期:1975-05-01 00:00:00

  • Expression of recombinant proteins in insect cells by their direct infection with Escherichia coli transformed with baculovirus bacmids.

    abstract::The baculovirus-insect cell expression system (BES), one of the most popular systems for expression of eukaryotic proteins, was known to have drawbacks such as laborious manipulation of large-size baculovirus bacmids and the transfection procedure. These problems could be eliminated by direct infection of eukaryotic c...

    journal_title:Acta virologica

    pub_type: 杂志文章

    doi:10.4149/av_2014_01_61

    authors: Yu X,Hua J,Hu G,Zhang J,Ma L

    更新日期:2014-01-01 00:00:00

  • JC polyomavirus reactivation is not associated with herpes zoster.

    abstract::Herpes zoster (HZ) is a neurocutaneous disease caused by Varicella-zoster virus (VZV) as a consequence of declined cell-mediated immunity, immune suppression and immunodeficiency. As reactivation of JC polyomavirus (JCPyV) might be linked with immunodeficiency or immunosuppressive therapy, the relationship between HZ ...

    journal_title:Acta virologica

    pub_type: 杂志文章

    doi:

    authors: Jeong BH,Park SJ,Koo DW,Kim YS

    更新日期:2006-01-01 00:00:00

  • Optimum conditions for the storage of potato virus S.

    abstract::The effect of storage conditions on the serological activity of two strains of potato virus S (PVS), Andean and the ordinary, was studied by ELISA. Virus purificates, infected leaves and their homogenates, stored in lyophilized, frozen and dissolved form at various temperatures were tested. Virus purificates were most...

    journal_title:Acta virologica

    pub_type: 杂志文章

    doi:

    authors: Cerovská N,Filigarová M

    更新日期:1995-02-01 00:00:00

  • Detection of influenza B virus in throat swabs using the polymerase chain reaction.

    abstract::An assay protocol based on exploiting the polymerase chain reaction (PCR) for the direct detection of influenza B virus in throat swabs is described. By the use of PCR with nested primers, it was possible to detect the virus in throat swabs. Dilution experiments showed that as little as 1 plaque forming unit of virus ...

    journal_title:Acta virologica

    pub_type: 杂志文章

    doi:

    authors: Yamada A,Imanishi J

    更新日期:1992-05-01 00:00:00

  • Detection of antibodies to the western and the eastern type of haemorrhagic fever with renal syndrome in patient's sera from Slovakia.

    abstract::We present serological evidence of infection with western and eastern type of haemorrhagic fever with renal syndrome (HFRS) virus in patient's sera from Slovakia by indirect immunofluorescence. Treatment of sera with 2-mercaptoethanol decreased levels of haemagglutination-inhibition (HI) antibodies to the eastern type...

    journal_title:Acta virologica

    pub_type: 杂志文章

    doi:

    authors: Gresíková M,Tkachenko EA,Sekeyová M

    更新日期:1990-08-01 00:00:00

  • Virus-induced decrease in the activity of serum blocking factor(s): a mechanism of activation of the autoreactive T effectors in graft versus host reaction and of controlling T suppressors.

    abstract::It has been shown that serum blocking factor(s) (SBF) previously detected in normal mice bind(s) to receptors on the surface membranes of virus-induced autoreactive T lymphocytes (ARTL) and to that of ARTL activity inhibiting T suppressors (TSar). The interaction of SBF with the receptors is reversible, H-2 restricted...

    journal_title:Acta virologica

    pub_type: 杂志文章

    doi:

    authors: Khozinsky VV,Semenov BF

    更新日期:1985-01-01 00:00:00

  • An enzyme linked immunoassay for estimation of antibodies to Newcastle disease virus strains.

    abstract::The sensitivity of enzyme linked immunoassay (ELISA) was compared to that of haemagglutination inhibition (HI) test in detection of humoral antibodies in birds vaccinated with Newcastle disease virus (NDV). The highest antibody titre at day 21 post-vaccination as detected by ELISA in the sera of vaccinated birds was 2...

    journal_title:Acta virologica

    pub_type: 杂志文章

    doi:

    authors: Mishra SC,Rai A,Jaiswal TN

    更新日期:1985-03-01 00:00:00

  • Cytotoxic T lymphocyte control during ectromelia (mousepox) virus infection: interaction between MHC-restricted cells analyzed by non-radioactive fluorometry.

    abstract::Cytotoxic T lymphocyte (CTL) activity of draining lymph node (DLN) cells isolated from BALB/c mice infected with ectromelia virus (EV) was examined using a fluorometric cell-mediated cytotoxicity (CMC) assay. Specific lysis of target cells A20 and EMT-6 primed with EV was demonstrated. The classical CD8+ cytolytic pat...

    journal_title:Acta virologica

    pub_type: 杂志文章

    doi:

    authors: Toka FN,Niemiałtowski MG,Spohr de Faundez I,Gieryńska M

    更新日期:1996-11-01 00:00:00

  • Histopathology and immunopathology of skin biopsy specimens in Mediterranean spotted fever.

    abstract::Histology of skin lesions and demonstration in them of Rickettsia conorii by direct immunofluorescence test (DIF) are presented in 13 patients with Mediterranean spotted fever (MSF). The lymphohistiocytic vasculitis which dominated the picture is not specific, however, it could be suggestive for the diagnosis of ricke...

    journal_title:Acta virologica

    pub_type: 杂志文章

    doi:

    authors: Dujella J,Morović M,Dzelalija B,Gverić M,Novaković S

    更新日期:1991-11-01 00:00:00

  • Enzyme-immunoassay in the diagnosis of human hepatitis A: specific and non-specific reactions.

    abstract::Solid-phase enzyme-immunoassay (EIA) used for the detection of hepatitis A virus (HAV) often reveals a non-specific activity which can be reduced or fully eliminated in the presence of normal serum. The factor responsible for this activity appeared to be a non-viral EIA-active material (NVEAM) that non-specifically re...

    journal_title:Acta virologica

    pub_type: 杂志文章

    doi:

    authors: Tolskaya EA,Savinskaya SS,Balayan MS,Andzhaparidze AG,Kolesnikova MS

    更新日期:1983-01-01 00:00:00

  • Coxiella burnetii antigens for detection of Q fever antibodies by ELISA in human sera.

    abstract::Different concentrations of chemically-treated (by potassium periodate oxidation or mild acid hydrolysis) purified phase I Coxiella burnetii (C.b.) corpuscles and natural (untreated) purified phase I and phase II C.b. corpuscles were compared by ELISA for detection of both phase I (directed to antigen 1) and phase II ...

    journal_title:Acta virologica

    pub_type: 杂志文章

    doi:

    authors: Kovácová E,Gallo J,Schramek S,Kazár J,Brezina R

    更新日期:1987-05-01 00:00:00

  • Hepatitis B vaccine alone or in combination with anti-HBs immunoglobulin in the perinatal prophylaxis of babies born to HBsAg carrier mothers.

    abstract::The efficacy of hepatitis B virus (HBV) vaccine alone (group I) or in combination with hepatitis B immunoglobulin (HBIG) (group II) for prevention of perinatal transmission of the virus was assessed in 21 and 24 neonates, respectively. 58 infants who could not be vaccinated constituted the control group. It was observ...

    journal_title:Acta virologica

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:

    authors: Sehgal A,Gupta I,Sehgal R,Ganguly NK

    更新日期:1992-08-01 00:00:00

  • Effects of monoparacoumarylputrescinium chloride on the hypersensitive reaction of Gomphrena globosa leaves to tomato bushy stunt virus.

    abstract::Monoparacoumarylputrescinium chloride (pCPH), supplied to detached leaves of Gomphrena globosa via the petiole, induced interference with tomato bushy stunt virus infection by reducing the number and size of the necrotic local lesions. The phenolic compound neither inactivated directly the virus in vitro, nor induced ...

    journal_title:Acta virologica

    pub_type: 杂志文章

    doi:

    authors: Pennazio S,Dellamonica G,Redolfi P

    更新日期:1981-03-01 00:00:00

  • Infectious full-length clones of plant viruses and their use for construction of viral vectors.

    abstract::In the last two decades, preparation of infectious RNA or DNA clones of plant viruses has transformed to a standard laboratory technique, providing an excellent tool for the research of viral gene functions and virus-host interactions. A full-length clone can be transformed in vitro to a viral vector expressing foreig...

    journal_title:Acta virologica

    pub_type: 杂志文章,评审

    doi:

    authors: Nagyová A,Subr Z

    更新日期:2007-01-01 00:00:00

  • Neopterin and soluble CD14 levels as indicators of immune activation in cases with low anti-HCV reactivity and true HCV infection.

    abstract::Neopterin and soluble CD14 (sCD14) are detected at high levels in hepatitis C virus (HCV) infections. We aimed to evaluate the role of these plasma immune activation biomarkers, for the indirect assessment of immune activation status of patients with low anti-HCV reactivity and a HCV infection. Low anti-HCV reactivity...

    journal_title:Acta virologica

    pub_type: 杂志文章,多中心研究

    doi:10.4149/av_2017_304

    authors: Habip Z,Sohrabi P,Saribas S,Caliskan R,Demirci M,Karakullukcu A,Atalik K,Yuksel P,Uysal O,Kosan E,Bahar Tokman H,Kocazeybek B

    更新日期:2017-01-01 00:00:00

  • Effect of cellular inhibitors on the infection of various susceptible cells with vesicular stomatitis virus.

    abstract::The effect of some cellular function inhibitors on adsorption and successive events of penetration of vesicular stomatitis virus to phylogenetically unrelated permissive cells was investigated. Treatment of HeLa, CER, EPC and Aedes albopictus cells with colcemide and cytochalasin D which affect cytoskeleton organizati...

    journal_title:Acta virologica

    pub_type: 杂志文章

    doi:

    authors: Superti F,Seganti L,Orsi N

    更新日期:1988-11-01 00:00:00

  • Experimental Hazara Virus infection in mice.

    abstract::Reproduction of Hazara virus in intracerebrally (ic) inoculated newborn white mice was studied by virological, serological and histological methods, electron microscopy and the direct fluorescent antibody technique. In the course of infection the virus was detectable in the blood, brain and some viscera, the largest a...

    journal_title:Acta virologica

    pub_type: 杂志文章

    doi:

    authors: Smirnova SE,Shestopalova NM,Reingold VN,Zubri GL,Chumakov MP

    更新日期:1977-03-01 00:00:00

  • Studies of some biological and molecular characteristics of avian influenza A/H7 (Hav1) subtypes.

    abstract::Current isolates of the subtypes H7N3 and H7N7 from 1979 to 1981 were examined and compared with the reference strains with regard to their antigenic variability and to their pathogenicity for birds and mammals in order to establish the potentiality of influenza A/H7 virus (Hav1) transmission from birds to mammals. Th...

    journal_title:Acta virologica

    pub_type: 杂志文章

    doi:

    authors: Sinnecker H,Egert J,Eggert HJ,Gogolin J,Gümpel S,Schrader C,Sinnecker R,Zilske E

    更新日期:1986-01-01 00:00:00