Detection of premalignant oral lesions in hamsters with an endoscopic fluorescence imaging system.

Abstract:

BACKGROUND:Various methods of detecting cancer with fluorescence have been developed. One type of fluorescence is based on the tumor-localizing properties of certain dyes. However, the phototoxicity of most known tumor-localizing dyes hinders the safe use of such diagnostic methods. The authors have developed a fluorescence imaging system to detect the distribution of a nontoxic dye, fluorescein, and they have evaluated the feasibility of the system by using it to detect oral dysplastic lesions in hamsters. METHODS:Dysplasia was induced in the cheek pouches of hamsters by application of the carcinogen 9,10-dimethyl-1,2-benzantracene. Fluorescein was administered to the hamsters either intravenously or orally before the fluorescence examination. The endoscopic fluorescence system produced dye-distribution images of both treated and control pouches. Two fluorescence images in different spectral regions were processed for each dye image. Biopsy material from both pouches was examined histopathologically. RESULTS:The accumulation of fluorescein was detected in 22 of 23 specimens containing dysplastic lesions. CONCLUSIONS:These results demonstrate the utility of this fluorescein accumulation method in the detection of dysplasia. The accumulation of fluorescein in dysplastic lesions may point to acidification of interstitial medium in such lesions.

journal_name

Cancer

journal_title

Cancer

authors

Lazarev VV,Roth RA,Kazakevich Y,Hang J

doi

10.1002/(sici)1097-0142(19990401)85:7<1421::aid-cn

subject

Has Abstract

pub_date

1999-04-01 00:00:00

pages

1421-9

issue

7

eissn

0008-543X

issn

1097-0142

pii

10.1002/(SICI)1097-0142(19990401)85:7<1421::AID-CN

journal_volume

85

pub_type

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