Abstract:
:Angiotensin-(1-7) [Ang-(1-7)] possesses novel biological functions that are distinct from angiotensin II (Ang II). In coronary arteries, the octapeptide Ang II and the heptapeptide Ang-(1-7) exert opposing actions. Ang II elicits vasoconstriction and Ang-(1-7) is a vasodilator. Ang-(1-7) elicits vasodilation by an endothelium-dependent release of nitric oxide. Further, the vasorelaxant activity is markedly attenuated by the bradykinin (BK) B2 receptor antagonist icatibant and does not appear to be associated with the synthesis and release of prostaglandins. Ang-(1-7) vasodilation is mediated by a non-AT1/AT2 receptor, since [Sar1Thr8]-Ang II, but neither candesartan, an AT1 receptor antagonist, nor PD123319, an AT2 receptor antagonist, blocked the response. Specific and high affinity binding of 125I-Ang-(1-7) to the endothelial layer of canine coronary arteries was demonstrated using in vitro emulsion autoradiography. Binding was effectively competed for by either unlabeled Ang-(1-7) or the specific Ang-(1-7) antagonist [D-Ala7]-Ang-(1-7). Additionally, Ang-(1-7) potentiated synergistically BK-induced vasodilation. The EC50 of BK vasodilation (2.45 +/- 0.51 nmol/L vs 0.37 +/- 0.08 nmol/L) was shifted 6.6-fold left-ward in the presence of 2 mumol/L concentration of Ang-(1-7). The potentiated response was specific for BK, since Ang-(1-7) did not augment the vasodilation produced by either acetylcholine or sodium nitroprusside; further, it was specific for Ang-(1-7), since neither Ang I nor Ang II augmented the BK response. In contrast to the vasodilator actions of Ang-(1-7), the potentiated response was not blocked by candesartan, PD123319 or [Sar1Thr8]-Ang II. Novel studies from our group demonstrate that Ang-(1-7) is both a substrate and inhibitor for angiotensin converting enzyme (ACE). Ang-(1-7) was shown to retard the degradation of 125I-[Tyr0]-BK in coronary rings. These studies describe novel actions of Ang-(1-7) as a vasodilator and a local synergistic modulator of kinin-induced vasodilation in coronary arteries.
journal_name
Biol Resjournal_title
Biological researchauthors
Brosnihan KB,Li P,Tallant EA,Ferrario CMsubject
Has Abstractpub_date
1998-01-01 00:00:00pages
227-34issue
3eissn
0716-9760issn
0717-6287journal_volume
31pub_type
杂志文章,评审abstract::By using the fluorescent Ca2+ indicator fura 2, submicromolar levels of intracellular Ca2+ have been detected in Trypanosoma cruzi different stages. The intracellular transport mechanisms involved in maintaining Ca2+ homeostasis in T. cruzi have been characterized by measuring Ca2+ transport in digitonin-permeabilized...
journal_title:Biological research
pub_type: 杂志文章
doi:
更新日期:1993-01-01 00:00:00
abstract::Duchenne muscular dystrophy (DMD) is secondary to loss-of-function mutations in the dystrophin gene. The causes underlying the progression of DMD, differential muscle involvement, and the discrepancies in phenotypes among species with the same genetic defect are not understood. The mdx mouse, an animal model with dyst...
journal_title:Biological research
pub_type: 杂志文章,评审
doi:10.4067/s0716-97602005000400010
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journal_title:Biological research
pub_type: 杂志文章
doi:10.1186/s40659-015-0057-0
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journal_title:Biological research
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doi:10.4067/s0716-97602000000200013
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journal_title:Biological research
pub_type: 临床试验,杂志文章
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journal_title:Biological research
pub_type: 杂志文章
doi:/S0716-97602008000200012
更新日期:2008-01-01 00:00:00
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journal_title:Biological research
pub_type: 杂志文章
doi:10.4067/s0716-97602003000300009
更新日期:2003-01-01 00:00:00
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journal_title:Biological research
pub_type: 杂志文章
doi:
更新日期:1998-01-01 00:00:00
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doi:10.1186/0717-6287-47-7
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journal_title:Biological research
pub_type: 杂志文章,评审
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pub_type: 杂志文章
doi:10.1186/s40659-016-0088-1
更新日期:2016-07-02 00:00:00
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journal_title:Biological research
pub_type: 杂志文章,评审
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journal_title:Biological research
pub_type: 杂志文章
doi:/S0716-97602009000400006
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journal_title:Biological research
pub_type: 杂志文章
doi:
更新日期:1993-01-01 00:00:00
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journal_title:Biological research
pub_type: 杂志文章
doi:/S0716-97602011000400008
更新日期:2011-01-01 00:00:00
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journal_title:Biological research
pub_type: 杂志文章,评审
doi:
更新日期:1993-01-01 00:00:00
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journal_title:Biological research
pub_type: 杂志文章
doi:/S0716-97602008000400005
更新日期:2008-01-01 00:00:00
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journal_title:Biological research
pub_type: 杂志文章
doi:10.4067/s0716-97602006000300010
更新日期:2006-01-01 00:00:00
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journal_title:Biological research
pub_type: 杂志文章
doi:
更新日期:1993-01-01 00:00:00
abstract::Peroxisomes are thought to be formed by division of pre-existing peroxisomes after the import of newly synthesized proteins. However, it has been recently suggested that the endoplasmic reticulum (ER) provides an alternative de novo mechanism for peroxisome biogenesis in some cells. To test a possible role of the ER-G...
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更新日期:2007-01-01 00:00:00
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journal_title:Biological research
pub_type: 杂志文章
doi:/S0716-97602009000200003
更新日期:2009-01-01 00:00:00
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更新日期:2013-01-01 00:00:00
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journal_title:Biological research
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更新日期:1994-01-01 00:00:00
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abstract:BACKGROUND:Tear desiccation on a glass surface followed by transmitted-light microscopy has served as diagnostic test for dry eye. Four distinctive morphological domains (zones I, II, III and transition band) have been recently recognized in tear microdesiccates. Physicochemical dissimilarities among those domains hamp...
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更新日期:2016-06-03 00:00:00