Abstract：
:Cyanobacteria display a large diversity of cellular forms ranging from unicellular to complex multicellular filaments or aggregates. Species in the group present a wide range of metabolic characteristics including the fixation of atmospheric nitrogen, resistance to extreme environments, production of hydrogen, secondary metabolites and exopolysaccharides. These characteristics led to the growing interest in cyanobacteria across the fields of ecology, evolution, cell biology and biotechnology. The number of available cyanobacterial genome sequences has increased considerably in recent years, with more than 140 fully sequenced genomes to date. Genetic engineering of cyanobacteria is widely applied to the model unicellular strains Synechocystis sp. PCC 6803 and Synechococcus elongatus PCC 7942. However the establishment of transformation protocols in many other cyanobacterial strains is challenging. One obstacle to the development of these novel model organisms is that many species have doubling times of 48 h or more, much longer than the bacterial models E. coli or B. subtilis. Furthermore, cyanobacterial defense mechanisms against foreign DNA pose a physical and biochemical barrier to DNA insertion in most strains. Here we review the various barriers to DNA uptake in the context of lateral gene transfer among microbes and the various mechanisms for DNA acquisition within the prokaryotic domain. Understanding the cyanobacterial defense mechanisms is expected to assist in the development and establishment of novel transformation protocols that are specifically suitable for this group.
journal_name
Biol Resjournal_title
Biological researchauthors
Stucken K,Koch R,Dagan Tdoi
10.4067/S0716-97602013000400009subject
Has Abstractpub_date
2013-01-01 00:00:00pages
373-82issue
4eissn
0716-9760issn
0717-6287pii
S0716-97602013000400009journal_volume
46pub_type
杂志文章,评审abstract：BACKGROUND:Psoriasis is a common chronic inflammatory skin disease. Keratinocytes hyperproliferation and excessive inflammatory response contribute to psoriasis pathogenesis. The agents able to attenuate keratinocytes hyperproliferation and excessive inflammatory response are considered to be potentially useful for pso...
journal_title：Biological research
pub_type： 杂志文章
doi：10.1186/s40659-020-00316-0
更新日期：2020-10-20 00:00:00
abstract：:Tumor growth is the result of combined cell proliferation overwhelming cell death and neoangiogenesis. This report shows CAM angiogenesis promoted by TA3 tumor supernatant with or without low dosis of betamethasone (Minimal antiangiogenic concentration: beta-MAAC). Methylcellulose discs instilled with 10 microliters o...
journal_title：Biological research
pub_type： 杂志文章
doi：10.4067/s0716-97602001000300010
更新日期：2001-01-01 00:00:00
abstract：BACKGROUND:Accumulating studies have demonstrated that high-mobility group A2 (HMGA2), an oncofetal protein, plays a role in tumor development and progression. However, the molecular role of HMGA2 in ovarian carcinoma is yet to be established. MicroRNAs (miRNAs), a group of small noncoding RNAs, negatively regulate gen...
journal_title：Biological research
pub_type： 杂志文章
doi：10.1186/s40659-018-0199-y
更新日期：2018-11-24 00:00:00
abstract：:Skeletal muscles have the potential to regenerate by activation of quiescent satellite cells, however, the molecular signature that governs satellite cells during muscle regeneration is not well defined. Myosin light chains (Myls) are sarcomere-related proteins as traditional regulator of muscle contraction. In this r...
journal_title：Biological research
pub_type： 杂志文章
doi：/S0716-97602009000100013
更新日期：2009-01-01 00:00:00
abstract：:Brain cells have a highly active oxidative metabolism, yet they contain only low to moderate superoxide dismutase and catalase activities. Thus, their antioxidant defenses rely mainly on cellular reduced glutathione levels. In this work, in cortical neurons we characterized viability and changes in reduced and oxidize...
journal_title：Biological research
pub_type： 杂志文章
doi：10.4067/s0716-97602006000100012
更新日期：2006-01-01 00:00:00
abstract：:Eight Chilean strains of Dunaliella salina obtained within a restricted geographic range, but exhibiting a high variability in their morphology, rate of growth and carotenogenic capacity, were analyzed by Random Amplified Polymorphic DNA (RAPD-PCR) Twenty of the 50 random primers (D, P, OPA and OPD series) that were t...
journal_title：Biological research
pub_type： 杂志文章
doi：10.4067/s0716-97602001000100012
更新日期：2001-01-01 00:00:00
abstract：BACKGROUND:Left ventricular diastolic dysfunction is one of the main characteristics of heart failure patients with a preserved left ventricular ejection fraction. As bilirubin is regarded as an important endogenous antioxidant molecule, serum total bilirubin levels were compared between heart failure patients with a p...
journal_title：Biological research
pub_type： 杂志文章
doi：10.1186/0717-6287-47-7
更新日期：2014-03-26 00:00:00
abstract：:The historical, lexical and conceptual issues embedded in stem cell biology are reviewed from technical, ethical, philosophical, judicial, clinical, economic and biopolitical perspectives. The mechanisms assigning the simultaneous capacity to self-renew and to differentiate to stem cells (immortal template DNA and asy...
journal_title：Biological research
pub_type： 历史文章,杂志文章,评审
doi：10.4067/S0716-97602012000300002
更新日期：2012-01-01 00:00:00
abstract：:The anti-inflammatory and redox protective effects of the citronellal (CT) were evaluated using in vivo and in vitro tests. Intraperitoneal (i.p.) administration of CT (50, 100, and 200 mg/kg) inhibited (p < 0.05) the carrageenan-induced leukocyte migration to the peritoneal cavity. Additionally, the carrageenan- and ...
journal_title：Biological research
pub_type： 杂志文章
doi：/S0716-97602011000400008
更新日期：2011-01-01 00:00:00
abstract：:This study examined the effects of estrogen supplementation on markers of neutrophil infiltration and damage in skeletal muscle of rats following ischemia. Male and female gonad-intact rats, with or without 14 days of estrogen supplementation were subjected to two hours of hind-limb ischemia and sacrificed at 24, 48 o...
journal_title：Biological research
pub_type： 杂志文章
doi：10.4067/s0716-97602005000200011
更新日期：2005-01-01 00:00:00
abstract：:Control of smooth muscle is vital for health. The major route to contraction is a rise in intracellular [Ca2+], determined by the entry and efflux of Ca2+ and release and re-uptake into the sarcoplasmic reticulum (SR). We review these processes in myometrium, to better understand excitation-contraction coupling and de...
journal_title：Biological research
pub_type： 杂志文章
doi：10.4067/s0716-97602004000400017
更新日期：2004-01-01 00:00:00
abstract：:Although there is extensive literature about the effects of stimulants on sustained attention tasks in attentional deficit/hyperactivity disorder (ADHD), little is known about the effect of these drugs on other attentional tasks involving different neural systems. In this study we measured the effect of stimulants on ...
journal_title：Biological research
pub_type： 杂志文章
doi：10.4067/s0716-97602004000300010
更新日期：2004-01-01 00:00:00
abstract：BACKGROUND:Papillary thyroid cancer (PTC) is the most common malignancy of all thyroid cancers. LncRNA LINC00460 has been proved to play roles in the oncogenesis and progression of various tumors, including papillary thyroid cancer. However, the potential molecular mechanism of LINC00460 in PTC is poorly investigated. ...
journal_title：Biological research
pub_type： 杂志文章
doi：10.1186/s40659-019-0269-9
更新日期：2019-12-23 00:00:00
abstract：:Shigella flexneri causes bacillary dysentery in humans. Essential to the establishment of the disease is the invasion of the colonic epithelial cells. Here we investigated the role of the lipopolysaccharide (LPS) O antigen in the ability of S. flexneri to adhere to and invade polarized Caco-2 cells. The S. flexneri 2a...
journal_title：Biological research
pub_type： 杂志文章
doi：10.4067/S0716-97602012000100003
更新日期：2012-01-01 00:00:00
abstract：:Hearing loss is the most common inherited sensorial deficiency in humans; about 1 in 1000 children suffer from severe or profound hearing loss at birth. Mutations in the GJB2 gene are the most common cause of prelingual, non-syndromic autosomal recessive deafness in many populations; the c.35delG mutation is the most ...
journal_title：Biological research
pub_type： 杂志文章
doi：10.4067/S0716-97602013000300003
更新日期：2013-01-01 00:00:00
abstract：:Red blood cell carbonic anhydrase (CA) activity has not been studied in high altitude natives. Because CA is an intraerythocytic enzyme and high altitude natives are polycythemic, it is important to know if the activity of CA per red cell volume is different from that of their sea level counterparts. Blood was collect...
journal_title：Biological research
pub_type： 杂志文章
doi：10.4067/s0716-97602000000300006
更新日期：2000-01-01 00:00:00
abstract：:The ascorbate-glutathione (ASC-GSH) cycle has an important role in defensive processes against oxidative damage generated by drought stress. In this study, the changes that take place in apoplastic and symplastic ASC-GSH cycle enzymes of the leaf and petiole were investigated under drought stress causing leaf rolling ...
journal_title：Biological research
pub_type： 杂志文章
doi：/S0716-97602009000300006
更新日期：2009-01-01 00:00:00
abstract：:By using the fluorescent Ca2+ indicator fura 2, submicromolar levels of intracellular Ca2+ have been detected in Trypanosoma cruzi different stages. The intracellular transport mechanisms involved in maintaining Ca2+ homeostasis in T. cruzi have been characterized by measuring Ca2+ transport in digitonin-permeabilized...
journal_title：Biological research
pub_type： 杂志文章
doi：
更新日期：1993-01-01 00:00:00
abstract：:Bovine median eminence contains a factor difference from gonadotropin-releasing hormone (GnRH) than increases basal luteinizing hormone (LH) secretion and potentiates GnRH-stimulated LH release. We compared the effect of hypothalamic neuropeptides on basal and GnRH-stimulated LH secretion using rat pituitary cells und...
journal_title：Biological research
pub_type： 杂志文章
doi：
更新日期：1994-01-01 00:00:00
abstract：BACKGROUND:Previous studies have shown that long noncoding RNA (lncRNA) LINC00483 was aberrantly expressed in human cancers, including gastric cancer. However, the regulatory mechanism of this lncRNA in gastric cancer remains largely unknown. The present study aimed to investigate the effect of LINC00483 on gastric can...
journal_title：Biological research
pub_type： 杂志文章
doi：10.1186/s40659-020-00283-6
更新日期：2020-04-15 00:00:00
abstract：BACKGROUND:Limonoids are highly oxygenated compounds with a prototypical structure. Their occurrence in the plant kingdom is mainly confined to plant families of Meliaceae and Rutaceae. Owing to their wide range of pharmacological and therapeutic properties, this study was aimed at investigating the potential nitric ox...
journal_title：Biological research
pub_type： 杂志文章
doi：10.1186/s40659-015-0049-0
更新日期：2015-10-13 00:00:00
abstract：:As regards their morphology and biology, tumours consist of heterogeneous cell populations. The cancer stem cell (CSC) hypothesis assumes that a tumour is hierarchically organized and not all of the cells are equally capable of generating descendants, similarly to normal tissue. The only cells being able to self-renew...
journal_title：Biological research
pub_type： 杂志文章,评审
doi：10.1186/0717-6287-47-66
更新日期：2014-12-10 00:00:00
abstract：:Thymulin is a well-characterized thymic hormone that exists as a nonapeptide coupled to equimolar amounts of Zn2+. Thymulin is known to have multiple biological roles, including T cell differentiation, immune regulation, and analgesic functions. It has been shown that thymulin is produced by the reticulo-epithelial ce...
journal_title：Biological research
pub_type： 杂志文章
doi：/S0716-97602010000300005
更新日期：2010-01-01 00:00:00
abstract：:Santiago de Chile has a high level of air pollution with ozone (O3), carbon monoxide (CO) and particles equal or smaller than 10 microns (PM10) usually exceeding the accepted standards. This situation should be noxious for the exposed population and particularly--in the case of O3 and PM10--for the respiratory system....
journal_title：Biological research
pub_type： 杂志文章
doi：
更新日期：1998-01-01 00:00:00
abstract：:Caffeic acid, rosmarinic acid and oligomers of caffeic acid with multiple catechol groups are all constituents of Salvia officinalis. Their antioxidant potential was investigated with regard to their radical scavenging activity and the stability and structure of the intermediate radicals. Pulse-radiolytic studies reve...
journal_title：Biological research
pub_type： 杂志文章
doi：10.4067/s0716-97602004000200017
更新日期：2004-01-01 00:00:00
abstract：BACKGROUND:The Hawthorn (Crateagus sp.) mostly occurs around the temperate region of the world with a high number of species, producing a fruit with numerous beneficial effects for human health. The aim of the study was to determine organic acid and sugar contents in the fruit of a number of hawthorn species grown in E...
journal_title：Biological research
pub_type： 杂志文章
doi：10.1186/0717-6287-47-21
更新日期：2014-05-30 00:00:00
abstract：:Peroxisomes are thought to be formed by division of pre-existing peroxisomes after the import of newly synthesized proteins. However, it has been recently suggested that the endoplasmic reticulum (ER) provides an alternative de novo mechanism for peroxisome biogenesis in some cells. To test a possible role of the ER-G...
journal_title：Biological research
pub_type： 杂志文章
doi：10.4067/s0716-97602007000200014
更新日期：2007-01-01 00:00:00
abstract：:Loss of Imprinting (LOI) of IGF2 and over-expressed IGF2 are associated with tumorigenesis. Our previous epidemiological study found a relatively high frequency of IGF2 LOI in healthy mid-gestation pregnant women. The aim of this study is to determine whether the expression of IGF2 is associated with its imprinting st...
journal_title：Biological research
pub_type： 杂志文章
doi：10.4067/S0716-97602012000400004
更新日期：2012-01-01 00:00:00
abstract：BACKGROUND:Although tropical climate of Thailand is suitably endowed with biodiversity of insects, flies of medical importance is not well investigated. Using information from literature search, fly survey approach and specialist's experience, we review database of Sarcophaga (Liosarcophaga) dux Thomson (Diptera: Sarco...
journal_title：Biological research
pub_type： 杂志文章,评审
doi：10.1186/0717-6287-47-14
更新日期：2014-04-01 00:00:00
abstract：:We review the development of our knowledge and interpretations of the intermediary metabolism of Trypanosoma (Schizotrypanum) cruzi. Already in the 1950's it was clearly established that when this organism was exposed to large external concentrations of carbohydrates it was unable to catabolize them completely, even i...
journal_title：Biological research
pub_type： 杂志文章,评审
doi：
更新日期：1993-01-01 00:00:00
