Abstract:
:Peroxisomes are thought to be formed by division of pre-existing peroxisomes after the import of newly synthesized proteins. However, it has been recently suggested that the endoplasmic reticulum (ER) provides an alternative de novo mechanism for peroxisome biogenesis in some cells. To test a possible role of the ER-Golgi transit in peroxisome biogenesis in mammalian cells, we evaluated the biogenesis of three peroxisomal membrane proteins (PMPs): ALDRP (adrenoleukodystrophy related protein), PMP70 and Pex3p in CHO cells. We constructed chimeric genes encoding these PMPs and green fluorescent protein (GFP), and transiently transfected them to wild type and mutant CHO cells, in which normal peroxisomes were replaced by peroxisomal membrane ghosts. The expressed proteins were targeted to peroxisomes and peroxisomal ghosts correctly in the presence or absence of Brefeldin A (BFA), a drug known to block the ER-Golgi transit. Furthermore, low temperature did not disturb the targeting of Pex3p-GFP to peroxisomes. We also constructed two chimeric proteins of PMPs containing an ER retention signal "DEKKMP": GFP-ALDRP-DEKKMP and myc- Pex3p-DEKKMP. These proteins were mostly targeted to peroxisomes. No colocalization with an ER maker was found. These results suggest that the classical ER-Golgi pathway does not play a major role in the biogenesis of mammalian PMPs.
journal_name
Biol Resjournal_title
Biological researchauthors
Toro A,Arredondo C,Córdova G,Araya C,Palacios JL,Venegas A,Morita M,Imanaka T,Santos MJdoi
10.4067/s0716-97602007000200014subject
Has Abstractpub_date
2007-01-01 00:00:00pages
231-49issue
2eissn
0716-9760issn
0717-6287pii
S0716-97602007000200014journal_volume
40pub_type
杂志文章abstract::Apoptosis is a means by which organisms dispose of unwanted cells without inducing an inflammatory response. Alterations in apoptosis is a common process by which cells become cancerous. Paradoxically, many cancer chemotherapeutics preferentially kill cancer cells by inducing apoptosis. Diacylglycerol is a lipid secon...
journal_title:Biological research
pub_type: 杂志文章,评审
doi:10.4067/s0716-97602002000200014
更新日期:2002-01-01 00:00:00
abstract::The neuropeptide oxytocin (OXT) is a key factor in the initiation and regulation of sociosexual behavior. The present study analyzes the effects of cohabitation and social challenge on plasma OXT concentration rates in guinea pig pairs in relation to male sociosexual behavior. The cohabitation phase lasted 3 days. On ...
journal_title:Biological research
pub_type: 杂志文章
doi:10.4067/s0716-97602006000200007
更新日期:2006-01-01 00:00:00
abstract::Although several histone variants have been studied in both animal and plant organisms, little is known about their distribution during processes that involve alterations in chromatin function, such as differentiation, dedifferentiation and hormone treatment. In this study we evaluated the ratio of each histone varian...
journal_title:Biological research
pub_type: 杂志文章
doi:/S0716-97602009000400006
更新日期:2009-01-01 00:00:00
abstract::As regards their morphology and biology, tumours consist of heterogeneous cell populations. The cancer stem cell (CSC) hypothesis assumes that a tumour is hierarchically organized and not all of the cells are equally capable of generating descendants, similarly to normal tissue. The only cells being able to self-renew...
journal_title:Biological research
pub_type: 杂志文章,评审
doi:10.1186/0717-6287-47-66
更新日期:2014-12-10 00:00:00
abstract:BACKGROUND:miR-214 was demonstrated to be upregulated in models of renal disease and promoted fibrosis in renal injury independent of TGF-β signaling in vivo. However, the detailed role of miR-214 in acute kidney injury (AKI) and its underlying mechanism are still largely unknown. METHODS:In this study, an I/R-induced...
journal_title:Biological research
pub_type: 杂志文章
doi:10.1186/s40659-018-0179-2
更新日期:2018-09-04 00:00:00
abstract::Interstitial fluid flow stress is one of the most important mechanical stimulations of bone cells under physiological conditions. Osteocytes and osteoblasts act as primary mechanosensors within bones, and in vitro are able to respond to fluid shear stress, both morphologically and functionally. However, there is littl...
journal_title:Biological research
pub_type: 杂志文章
doi:10.4067/S0716-97602012000200008
更新日期:2012-01-01 00:00:00
abstract::The development of plant transformation in the mid-1980s and of many new tools for cell biology, molecular genetics, and biochemistry has resulted in enormous progress in plant biology in the past decade. With the completion of the genome sequence of Arabidopsis thaliana just around the corner, we can expect even fast...
journal_title:Biological research
pub_type: 杂志文章,评审
doi:
更新日期:1999-01-01 00:00:00
abstract:BACKGROUND:The role of interleukin family in colon cancer remained controversial. The purpose of this study was to investigate the association between interleukin family and colon cancer progression through bioinformatics methods and to validate such association in clinical patients. METHODS:A total of 15 differential...
journal_title:Biological research
pub_type: 杂志文章
doi:10.1186/s40659-020-00287-2
更新日期:2020-05-07 00:00:00
abstract::Vasopressin and bradykinin are two of the most important peptides in regulating vascular tone, water, and ionic balance in the body, and thus they play a key role in controlling blood pressure. In addition to being a potent vasoconstrictor, Vasopressin also has an antidiuretic activity in the kidney, whereas kinins re...
journal_title:Biological research
pub_type: 杂志文章,评审
doi:
更新日期:1999-01-01 00:00:00
abstract::Unilateral brain damage which includes the hippocampus leads to memory impairments consistent with hemispheric specialization on the same side. Damage to the corpus callosum, the major connecting pathway between the left and right hemispheres, also leads to memory impairments. This suggests both hemispheric specializa...
journal_title:Biological research
pub_type: 杂志文章,评审
doi:
更新日期:1995-01-01 00:00:00
abstract::The purpose of this study was to carry out a bibliometric analysis of the articles published in Chile during 1987-2006 that were categorized into the Plant Science discipline by ISI. The data was extracted from the Web of Science datábase, obtaining a total of 911 articles for analysis. Almost half of the articles wer...
journal_title:Biological research
pub_type: 杂志文章
doi:/S0716-97602008000200002
更新日期:2008-01-01 00:00:00
abstract::Bilateral aspiration lesions of the fimbria-fornix were performed in 10 male Sprague Dawley rats weighing 240-300 g under chloral hydrate narcose (420 mg/kg). Another 9 animals were operated in the same way, but no aspiration was carried out to constitute a control group. A week after surgery recording and stimulation...
journal_title:Biological research
pub_type: 杂志文章
doi:
更新日期:1996-01-01 00:00:00
abstract:BACKGROUND:CCL2 was up-regulated in neurons and involved in microglia activation and neurological decline in mice suffering from hepatic encephalopathy (HE). However, no data exist concerning the effect of neuron-derived CCL2 on microglia activation in vitro. METHODS:The rats were pretreated with CCL2 receptor inhibit...
journal_title:Biological research
pub_type: 杂志文章
doi:10.1186/s40659-017-0130-y
更新日期:2017-09-04 00:00:00
abstract::This study examined the effects of estrogen supplementation on markers of neutrophil infiltration and damage in skeletal muscle of rats following ischemia. Male and female gonad-intact rats, with or without 14 days of estrogen supplementation were subjected to two hours of hind-limb ischemia and sacrificed at 24, 48 o...
journal_title:Biological research
pub_type: 杂志文章
doi:10.4067/s0716-97602005000200011
更新日期:2005-01-01 00:00:00
abstract::The ascorbate-glutathione (ASC-GSH) cycle has an important role in defensive processes against oxidative damage generated by drought stress. In this study, the changes that take place in apoplastic and symplastic ASC-GSH cycle enzymes of the leaf and petiole were investigated under drought stress causing leaf rolling ...
journal_title:Biological research
pub_type: 杂志文章
doi:/S0716-97602009000300006
更新日期:2009-01-01 00:00:00
abstract::After the progress made during the genomics era, bioinformatics was tasked with supporting the flow of information generated by nanobiotechnology efforts. This challenge requires adapting classical bioinformatic and computational chemistry tools to store, standardize, analyze, and visualize nanobiotechnological inform...
journal_title:Biological research
pub_type: 杂志文章
doi:10.4067/S0716-97602011000100006
更新日期:2011-01-01 00:00:00
abstract::Eight Chilean strains of Dunaliella salina obtained within a restricted geographic range, but exhibiting a high variability in their morphology, rate of growth and carotenogenic capacity, were analyzed by Random Amplified Polymorphic DNA (RAPD-PCR) Twenty of the 50 random primers (D, P, OPA and OPD series) that were t...
journal_title:Biological research
pub_type: 杂志文章
doi:10.4067/s0716-97602001000100012
更新日期:2001-01-01 00:00:00
abstract:BACKGROUND:Psoralen is a coumarin-like and coumarin-related benzofuran glycoside, which is a commonly used traditional Chinese medicine to treat patients with kidney and spleen-yang deficiency symptom. Psoralen has been reported to show estrogen-like activity, antioxidant activity, osteoblastic proliferation accelerati...
journal_title:Biological research
pub_type: 杂志文章
doi:10.1186/s40659-019-0241-8
更新日期:2019-07-05 00:00:00
abstract::Tumor growth is the result of combined cell proliferation overwhelming cell death and neoangiogenesis. This report shows CAM angiogenesis promoted by TA3 tumor supernatant with or without low dosis of betamethasone (Minimal antiangiogenic concentration: beta-MAAC). Methylcellulose discs instilled with 10 microliters o...
journal_title:Biological research
pub_type: 杂志文章
doi:10.4067/s0716-97602001000300010
更新日期:2001-01-01 00:00:00
abstract::X-Ray diffraction is the only method currently available for the determination of the detailed three-dimensional structure of macromolecules. In this article, the principles behind the use of X-ray crystallography in the study of protein structure are presented. The results obtained in its application to several enzym...
journal_title:Biological research
pub_type: 杂志文章,评审
doi:
更新日期:1996-01-01 00:00:00
abstract:BACKGROUND:Psoriasis is a common chronic inflammatory skin disease. Keratinocytes hyperproliferation and excessive inflammatory response contribute to psoriasis pathogenesis. The agents able to attenuate keratinocytes hyperproliferation and excessive inflammatory response are considered to be potentially useful for pso...
journal_title:Biological research
pub_type: 杂志文章
doi:10.1186/s40659-020-00316-0
更新日期:2020-10-20 00:00:00
abstract::Many motor skills, once acquired, are stored over a long time period, probably sustained by permanent neuronal changes. Thus, in this paper we have investigated with quantitative stereology the generation and persistence of neuronal density changes in primary motor cortex (MI) following motor skill learning (skilled r...
journal_title:Biological research
pub_type: 杂志文章
doi:/S0716-97602008000400005
更新日期:2008-01-01 00:00:00
abstract::Brain cells have a highly active oxidative metabolism, yet they contain only low to moderate superoxide dismutase and catalase activities. Thus, their antioxidant defenses rely mainly on cellular reduced glutathione levels. In this work, in cortical neurons we characterized viability and changes in reduced and oxidize...
journal_title:Biological research
pub_type: 杂志文章
doi:10.4067/s0716-97602006000100012
更新日期:2006-01-01 00:00:00
abstract::The objective of this study was to test the hypothesis that root of maguey (Agave salmiana Otto ex Salm-Dyck) seedlings reacts during the first 24 h to low substrate water potential (PsiW), by anatomical modifications. Three-4 cm root length seedlings were planted in vermiculite for 24 h at PsiW between -0.03 and -2.3...
journal_title:Biological research
pub_type: 杂志文章
doi:/S0716-97602009000200013
更新日期:2009-01-01 00:00:00
abstract::Thymulin is a well-characterized thymic hormone that exists as a nonapeptide coupled to equimolar amounts of Zn2+. Thymulin is known to have multiple biological roles, including T cell differentiation, immune regulation, and analgesic functions. It has been shown that thymulin is produced by the reticulo-epithelial ce...
journal_title:Biological research
pub_type: 杂志文章
doi:/S0716-97602010000300005
更新日期:2010-01-01 00:00:00
abstract::Breast cancer is the most common malignancy among women. Chilean studies reveal that this cancer presents the third highest mortality rate. A family history of breast cancer is one of the major risk factors for the development of this disease. BRCA1 and BRCA2 are the two main hereditary breast cancer susceptibility ge...
journal_title:Biological research
pub_type: 杂志文章
doi:10.4067/s0716-97602002000100011
更新日期:2002-01-01 00:00:00
abstract::Galectins (S-type or S-Lac lectins) are a well-defined family of beta-galactoside animal lectins characterized by a high sequence homology in the carbohydrate-binding domain. We have previously purified and characterized the S-type lectin from the ovary of the toad Bufo arenarum. In this study, we purified the S-type ...
journal_title:Biological research
pub_type: 杂志文章
doi:
更新日期:1997-01-01 00:00:00
abstract:BACKGROUND:The Hawthorn (Crateagus sp.) mostly occurs around the temperate region of the world with a high number of species, producing a fruit with numerous beneficial effects for human health. The aim of the study was to determine organic acid and sugar contents in the fruit of a number of hawthorn species grown in E...
journal_title:Biological research
pub_type: 杂志文章
doi:10.1186/0717-6287-47-21
更新日期:2014-05-30 00:00:00
abstract::The regeneration of natural tetraploid T. pratense, originated from Erzurum-Turkey, is reported in this study. This plant has low seed setting and hard seed problems due to polyploidy. Hypocotyl, cotyledon, apical meristems, epicotyl and young primary leaves were inoculated on MS and PC-L2 media containing different c...
journal_title:Biological research
pub_type: 杂志文章
doi:/S0716-97602008000100004
更新日期:2008-01-01 00:00:00
abstract::The major cysteine proteinase from Trypanosoma cruzi, cruzipain, can be obtained essentially homogeneous, starting from crude epimastigote extracts, in one step, by affinity chromatography on Cystatin-Sepharose (specific for cysteine proteinases) or ConA-Sepharose (specific for high mannose N-linked glycoproteins). Th...
journal_title:Biological research
pub_type: 杂志文章
doi:
更新日期:1993-01-01 00:00:00