Abstract:
:By using the fluorescent Ca2+ indicator fura 2, submicromolar levels of intracellular Ca2+ have been detected in Trypanosoma cruzi different stages. The intracellular transport mechanisms involved in maintaining Ca2+ homeostasis in T. cruzi have been characterized by measuring Ca2+ transport in digitonin-permeabilized cells. Two intracellular calcium transport systems have been detected. Ca2+ uptake by the mitochondria occurs by an electrophoretic mechanism, is inhibited by antimycin A, FCCP, and ruthenium red, and stimulated by respiratory substrates, phosphate and acetate. This pool has a high capacity and low affinity for Ca2+ and is able to buffer external Ca2+ at concentrations in the range of 0.6-0.7 microM. Ca2+ uptake by the endoplasmic reticulum is inhibited by high concentrations of vanadate and anticalmodulin agents, and stimulated by ATP. This pool has a low capacity and a high affinity for Ca2+ and is able to buffer external Ca2+ at concentrations in the range of 0.05-1.0 microM. In addition, calmodulin has been purified from T. cruzi epimastigotes and shown to stimulate the homologous plasma membrane Ca(2+)-ATPase and cyclic-AMP phosphodiesterase. The gene encoding this protein has been cloned and sequenced and shown to have a great homology to mammalian calmodulin. The role of the plasma membrane of T. cruzi in the regulation of [Ca2+]i has been studied using fura 2-loaded epimastigotes or plasma membrane vesicles prepared from epimastigotes. Plasma membrane vesicles transport Ca2+ in the presence of Mg2+ and have a high affinity, vanadate-sensitive (Ca(2+)-Mg2+)-ATPase with an apparent Km for free Ca2+ of 0.3 microM.(ABSTRACT TRUNCATED AT 250 WORDS)
journal_name
Biol Resjournal_title
Biological researchauthors
Docampo Rsubject
Has Abstractpub_date
1993-01-01 00:00:00pages
189-96issue
1-2eissn
0716-9760issn
0717-6287journal_volume
26pub_type
杂志文章abstract::The major cysteine proteinase from Trypanosoma cruzi, cruzipain, can be obtained essentially homogeneous, starting from crude epimastigote extracts, in one step, by affinity chromatography on Cystatin-Sepharose (specific for cysteine proteinases) or ConA-Sepharose (specific for high mannose N-linked glycoproteins). Th...
journal_title:Biological research
pub_type: 杂志文章
doi:
更新日期:1993-01-01 00:00:00
abstract:BACKGROUND:α-Farnesene is a volatile sesquiterpene synthesized by the plant mevalonate (MVA) pathway through the action of α-farnesene synthase. The α-farnesene synthase 1 (MdAFS1) gene was isolated from apple peel (var. white winter pearmain), and transformed into tobacco (Nicotiana tabacum NC89). The transgenic plant...
journal_title:Biological research
pub_type: 杂志文章
doi:10.1186/s40659-016-0088-1
更新日期:2016-07-02 00:00:00
abstract::The development of plant transformation in the mid-1980s and of many new tools for cell biology, molecular genetics, and biochemistry has resulted in enormous progress in plant biology in the past decade. With the completion of the genome sequence of Arabidopsis thaliana just around the corner, we can expect even fast...
journal_title:Biological research
pub_type: 杂志文章,评审
doi:
更新日期:1999-01-01 00:00:00
abstract::The aim of this study was to investigate the in vitro cytotoxic activity of total extract of MeOH (70%) and partition fractions of hexan, chloroform (CHCL3), ethylacetate (EtOAc) and MeOH-H2O of brown algae species (Sargassum swartzii, Cystoseira myrica, Colpomenia sinuosa) found in the Persian Gulf against in differe...
journal_title:Biological research
pub_type: 杂志文章
doi:/S0716-97602010000100005
更新日期:2010-01-01 00:00:00
abstract::As regards their morphology and biology, tumours consist of heterogeneous cell populations. The cancer stem cell (CSC) hypothesis assumes that a tumour is hierarchically organized and not all of the cells are equally capable of generating descendants, similarly to normal tissue. The only cells being able to self-renew...
journal_title:Biological research
pub_type: 杂志文章,评审
doi:10.1186/0717-6287-47-66
更新日期:2014-12-10 00:00:00
abstract::The purpose of this study was to carry out a bibliometric analysis of the articles published in Chile during 1987-2006 that were categorized into the Plant Science discipline by ISI. The data was extracted from the Web of Science datábase, obtaining a total of 911 articles for analysis. Almost half of the articles wer...
journal_title:Biological research
pub_type: 杂志文章
doi:/S0716-97602008000200002
更新日期:2008-01-01 00:00:00
abstract:BACKGROUND:Understanding the molecular basis underlying the formation of bone-forming osteocytes and lipid-storing adipocytes will help provide insights into the cause of disorders originating in stem/progenitor cells and develop therapeutic treatments for bone- or adipose-related diseases. In this study, the role of R...
journal_title:Biological research
pub_type: 杂志文章
doi:10.1186/s40659-017-0148-1
更新日期:2017-12-26 00:00:00
abstract::Santiago de Chile has a high level of air pollution with ozone (O3), carbon monoxide (CO) and particles equal or smaller than 10 microns (PM10) usually exceeding the accepted standards. This situation should be noxious for the exposed population and particularly--in the case of O3 and PM10--for the respiratory system....
journal_title:Biological research
pub_type: 杂志文章
doi:
更新日期:1998-01-01 00:00:00
abstract::Interstitial fluid flow stress is one of the most important mechanical stimulations of bone cells under physiological conditions. Osteocytes and osteoblasts act as primary mechanosensors within bones, and in vitro are able to respond to fluid shear stress, both morphologically and functionally. However, there is littl...
journal_title:Biological research
pub_type: 杂志文章
doi:10.4067/S0716-97602012000200008
更新日期:2012-01-01 00:00:00
abstract:BACKGROUND:Asthma is a complex disease influenced by multiple genetic and environmental factors. While Madeira has the highest prevalence of asthma in Portugal (14.6%), the effect of both genetic and environmental factors in this population has never been assessed. We categorized 98 asthma patients according to the Glo...
journal_title:Biological research
pub_type: 杂志文章
doi:10.1186/0717-6287-47-40
更新日期:2014-09-04 00:00:00
abstract::Treatment of tumor-bearing mice with LD12.5 values of iodoacetate; IAA (1.84 mg/100 g b.w.) and/or dimethylsulphoxide; DMSO (350 mg/100 g b.w.) significantly increased the cumulative mean survival time and percentage of survivors and reduced the mean tumor weight, compared to tumor-bearing controls, however, a more pr...
journal_title:Biological research
pub_type: 杂志文章
doi:10.4067/s0716-97602003000200015
更新日期:2003-01-01 00:00:00
abstract::Studies of kallikrein-kinin systems since the 1930's have now led to substantial insight into the importance of this vasoactive system in the regulation of cardiovascular and renal function. It now seems clear that before long, these insights will lead to new therapeutic attacks upon diseases of the cardiovascular sys...
journal_title:Biological research
pub_type: 杂志文章,评审
doi:
更新日期:1998-01-01 00:00:00
abstract::Renal response to atrial natriuretic peptide in chronic cholestasis was studied in anaesthetized rats and in their isolated perfused kidneys. Cholestasis was induced by bile duct section after ligature, while controls were sham operated. Three weeks after surgery, cholestatic rats showed moderate arterial hypotension,...
journal_title:Biological research
pub_type: 杂志文章
doi:
更新日期:1993-01-01 00:00:00
abstract::Tumor growth is the result of combined cell proliferation overwhelming cell death and neoangiogenesis. This report shows CAM angiogenesis promoted by TA3 tumor supernatant with or without low dosis of betamethasone (Minimal antiangiogenic concentration: beta-MAAC). Methylcellulose discs instilled with 10 microliters o...
journal_title:Biological research
pub_type: 杂志文章
doi:10.4067/s0716-97602001000300010
更新日期:2001-01-01 00:00:00
abstract::The aim of this study is to describe the ultrastructural changes of the egg apparatus associated with fertilisation of the natural tetraploid Trifolium pratense. The pollen tube enters one of the synergids through the filliform apparatus from the micropyle. Before the entry of the pollen tube into the embryo sac, one ...
journal_title:Biological research
pub_type: 杂志文章
doi:/S0716-97602009000100003
更新日期:2009-01-01 00:00:00
abstract::The hypothesis that the vacation-study-expectancy scholar regime produces most of the monthly rhythm of the age at menarche (AaM) was tested. Studies on monthly menarche incidence (MI) refuted climatic factors as a main factor in this rhythm, and indicated that the main factor of this rhythm is the succession of expec...
journal_title:Biological research
pub_type: 杂志文章,评审
doi:10.4067/S0716-97602013000100003
更新日期:2013-01-01 00:00:00
abstract::Hematopoietic stem cell transplantation is the accepted therapy of choice for a variety of malignant and non-malignant diseases in children and adults. Initially developed as rescue therapy for a patient with cancer after high doses of chemotherapy and radiation as well as the correction of severe deficiencies in the ...
journal_title:Biological research
pub_type: 历史文章,杂志文章
doi:10.4067/S0716-97602012000300012
更新日期:2012-01-01 00:00:00
abstract:BACKGROUND:The role of interleukin family in colon cancer remained controversial. The purpose of this study was to investigate the association between interleukin family and colon cancer progression through bioinformatics methods and to validate such association in clinical patients. METHODS:A total of 15 differential...
journal_title:Biological research
pub_type: 杂志文章
doi:10.1186/s40659-020-00287-2
更新日期:2020-05-07 00:00:00
abstract::In the last few decades, several works on event related potentials (ERPs hereafter) during sleep have been reported. In spite of numerous studies, clear methodological rules for this kind of study are often missing, making it difficult to valorize the scope of these results. We propose here a description of methodolog...
journal_title:Biological research
pub_type: 杂志文章
doi:/S0716-97602008000300004
更新日期:2008-01-01 00:00:00
abstract:BACKGROUND:miR-214 was demonstrated to be upregulated in models of renal disease and promoted fibrosis in renal injury independent of TGF-β signaling in vivo. However, the detailed role of miR-214 in acute kidney injury (AKI) and its underlying mechanism are still largely unknown. METHODS:In this study, an I/R-induced...
journal_title:Biological research
pub_type: 杂志文章
doi:10.1186/s40659-018-0179-2
更新日期:2018-09-04 00:00:00
abstract::The regeneration of natural tetraploid T. pratense, originated from Erzurum-Turkey, is reported in this study. This plant has low seed setting and hard seed problems due to polyploidy. Hypocotyl, cotyledon, apical meristems, epicotyl and young primary leaves were inoculated on MS and PC-L2 media containing different c...
journal_title:Biological research
pub_type: 杂志文章
doi:/S0716-97602008000100004
更新日期:2008-01-01 00:00:00
abstract::Bovine median eminence contains a factor difference from gonadotropin-releasing hormone (GnRH) than increases basal luteinizing hormone (LH) secretion and potentiates GnRH-stimulated LH release. We compared the effect of hypothalamic neuropeptides on basal and GnRH-stimulated LH secretion using rat pituitary cells und...
journal_title:Biological research
pub_type: 杂志文章
doi:
更新日期:1994-01-01 00:00:00
abstract:BACKGROUND:The study was conducted to evaluate the in vitro thrombolytic activity, and in vivo analgesic, anti-inflammatory and antipyretic potentials of different hydrocarbon soluble extracts of Litsea glutinosa leaves for the first time widely used in the folkloric treatments in Bangladesh. This work aimed to create ...
journal_title:Biological research
pub_type: 杂志文章
doi:10.1186/0717-6287-47-56
更新日期:2014-10-29 00:00:00
abstract:BACKGROUND:CCL2 was up-regulated in neurons and involved in microglia activation and neurological decline in mice suffering from hepatic encephalopathy (HE). However, no data exist concerning the effect of neuron-derived CCL2 on microglia activation in vitro. METHODS:The rats were pretreated with CCL2 receptor inhibit...
journal_title:Biological research
pub_type: 杂志文章
doi:10.1186/s40659-017-0130-y
更新日期:2017-09-04 00:00:00
abstract::Extracts prepared from seeds of Manilkara zapota, Anona squamosa, and Tamarindus indica were screened for their antibacterial activity by disc diffusion and broth dilution methods. Acetone and methanol extracts of T. indica seeds were found active against both gram-positive and gram-negative organisms. MIC values of p...
journal_title:Biological research
pub_type: 杂志文章
doi:/S0716-97602010000200003
更新日期:2010-01-01 00:00:00
abstract:BACKGROUND:Prognosis remains one of most crucial determinants of gastric cancer (GC) treatment, but current methods do not predict prognosis accurately. Identification of additional biomarkers is urgently required to identify patients at risk of poor prognoses. METHODS:Tissue microarrays were used to measure expressio...
journal_title:Biological research
pub_type: 杂志文章
doi:10.1186/s40659-019-0249-0
更新日期:2019-08-09 00:00:00
abstract::Changes in the redox state of the intracellular ryanodine receptor/Ca2+ release channels of skeletal and cardiac muscle or brain cortex neurons affect their activity. In particular, agents that oxidize or alkylate free SH residues of the channel protein strongly enhance Ca(2+)-induced Ca2+ release, whereas reducing ag...
journal_title:Biological research
pub_type: 杂志文章,评审
doi:10.4067/s0716-97602004000400007
更新日期:2004-01-01 00:00:00
abstract::The aim of this study was to compare the effects of Mediterranean-type diet (MD), high-fat diet (HFD), and red wine supplementation on plasma concentration of emergent haemostatic cardiovascular risk factors (HCVRF) and on variables of primary haemostasis (bleeding time, plasma von Willebrand factor and platelet aggre...
journal_title:Biological research
pub_type: 临床试验,杂志文章
doi:10.4067/s0716-97602004000200007
更新日期:2004-01-01 00:00:00
abstract:BACKGROUND:chronic hypoxia increases basal ventilation and pulmonary vascular resistance, with variable changes in arterial blood pressure and heart rate, but it's impact on heart rate variability and autonomic regulation have been less well examined. We studied changes in arterial blood pressure, heart rate and heart ...
journal_title:Biological research
pub_type: 杂志文章
doi:10.1186/s40659-018-0207-2
更新日期:2018-12-20 00:00:00
abstract::The neuropeptide oxytocin (OXT) is a key factor in the initiation and regulation of sociosexual behavior. The present study analyzes the effects of cohabitation and social challenge on plasma OXT concentration rates in guinea pig pairs in relation to male sociosexual behavior. The cohabitation phase lasted 3 days. On ...
journal_title:Biological research
pub_type: 杂志文章
doi:10.4067/s0716-97602006000200007
更新日期:2006-01-01 00:00:00