Plant regeneration of natural tetraploid Trifolium Hum pratense L.

Abstract:

:The regeneration of natural tetraploid T. pratense, originated from Erzurum-Turkey, is reported in this study. This plant has low seed setting and hard seed problems due to polyploidy. Hypocotyl, cotyledon, apical meristems, epicotyl and young primary leaves were inoculated on MS and PC-L2 media containing different concentrations of BAP and NAA as growth regulators. The best shoot formation has been observed on explants initiated from apical meristem placed on PC-L2 medium that includes 2 mg dm(-3) BAP and 1 mg dm(-3) NAA. 94.4% of the shoots originated from calli were rooted on PC-L2 medium with 1 mg dm(-3) NAA. In vitro organogenesis has been accomplished in the natural tetraploid T. pratense regenerated plants successively transferred to the field.

journal_name

Biol Res

journal_title

Biological research

authors

Cölgeçen H,Toker MC

doi

/S0716-97602008000100004

subject

Has Abstract

pub_date

2008-01-01 00:00:00

pages

25-31

issue

1

eissn

0716-9760

issn

0717-6287

pii

S0716-97602008000100004

journal_volume

41

pub_type

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