Abstract:
:The regeneration of natural tetraploid T. pratense, originated from Erzurum-Turkey, is reported in this study. This plant has low seed setting and hard seed problems due to polyploidy. Hypocotyl, cotyledon, apical meristems, epicotyl and young primary leaves were inoculated on MS and PC-L2 media containing different concentrations of BAP and NAA as growth regulators. The best shoot formation has been observed on explants initiated from apical meristem placed on PC-L2 medium that includes 2 mg dm(-3) BAP and 1 mg dm(-3) NAA. 94.4% of the shoots originated from calli were rooted on PC-L2 medium with 1 mg dm(-3) NAA. In vitro organogenesis has been accomplished in the natural tetraploid T. pratense regenerated plants successively transferred to the field.
journal_name
Biol Resjournal_title
Biological researchauthors
Cölgeçen H,Toker MCdoi
/S0716-97602008000100004subject
Has Abstractpub_date
2008-01-01 00:00:00pages
25-31issue
1eissn
0716-9760issn
0717-6287pii
S0716-97602008000100004journal_volume
41pub_type
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