Abstract：
BACKGROUND:Ubiquitin specific peptidase 39 (USP39), an essential factor in the assembly of the mature spliceosome complex, has an aberrant expression in several cancer. However, its function and the corresponding mechanism on human osteosarcoma has not been fully explored yet. METHODS:The mRNA and DNA copies of USP39 were increased in osteosarcoma cancer tissues compared with the one in human normal tissues according to datasets from the publicly available Oncomine database. A further western blot analysis also demonstrated an aberrant endogenous expression of USP39 in three different osteosarcoma cells. Then lentivirus-mediated short hairpin RNA (shRNA) was designed to silence USP39 in human osteosarcoma cell line U2OS, which is used to test the impact of USP39-silencing on cellular proliferation, colony formation, cell cycle distribution and apoptosis. RESULTS:Knockdown of USP39 expression in U2OS cell significantly decreased cell proliferation, impaired colony formation ability. A further analysis indicated suppression of USP39 arrested cell cycle progression at G2/M phase via p21 dependent way. In addition, the results of Annexin V/7-AAD staining suggested the knockdown of USP39 could promote U2OS cell apoptosis through PARP cleavage. CONCLUSIONS:These results uncover the critical role of USP39 in regulating cancer cell mitosis and indicate USP39 is critical for osteosarcoma tumorigenesis.
journal_name
Biol Resjournal_title
Biological researchauthors
Gan Z,Han K,Lin S,Hu H,Shen Z,Min Ddoi
10.1186/s40659-017-0121-zsubject
Has Abstractpub_date
2017-04-12 00:00:00pages
15issue
1eissn
0716-9760issn
0717-6287pii
10.1186/s40659-017-0121-zjournal_volume
50pub_type
杂志文章abstract：:Hepcidin (Hepc) is a 25 amino acid cationic peptide with broad antibacterial and antifungal actions. A likely role for Hepc in iron metabolism was suggested by the observation that mice having disruption of the gene encoding the transcription factor USF2 failed to produce Hepc mRNA and developed spontaneous visceral i...
journal_title：Biological research
pub_type： 杂志文章
doi：10.4067/s0716-97602006000100022
更新日期：2006-01-01 00:00:00
abstract：:The present work was intended to evaluate the preparation of antigens, as well as the production and characterization of anti cAMP and cGMP antibodies. Such antibodies were obtained from rabbits, and we used 2'O-succinyl cyclic nucleotide derivate, conjugated with human serum albumin, as antigen. The characterization ...
journal_title：Biological research
pub_type： 杂志文章
doi：
更新日期：1994-01-01 00:00:00
abstract：BACKGROUND:Left ventricular diastolic dysfunction is one of the main characteristics of heart failure patients with a preserved left ventricular ejection fraction. As bilirubin is regarded as an important endogenous antioxidant molecule, serum total bilirubin levels were compared between heart failure patients with a p...
journal_title：Biological research
pub_type： 杂志文章
doi：10.1186/0717-6287-47-7
更新日期：2014-03-26 00:00:00
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journal_title：Biological research
pub_type： 杂志文章,评审
doi：10.4067/s0716-97602002000200004
更新日期：2002-01-01 00:00:00
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journal_title：Biological research
pub_type： 杂志文章
doi：10.1186/s40659-015-0054-3
更新日期：2015-11-19 00:00:00
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journal_title：Biological research
pub_type： 杂志文章
doi：10.4067/S0716-97602012000100003
更新日期：2012-01-01 00:00:00
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journal_title：Biological research
pub_type： 历史文章,杂志文章
doi：10.4067/S0716-97602012000300012
更新日期：2012-01-01 00:00:00
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journal_title：Biological research
pub_type： 杂志文章
doi：10.1186/s40659-017-0141-8
更新日期：2017-10-17 00:00:00
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journal_title：Biological research
pub_type： 杂志文章
doi：/S0716-97602008000400005
更新日期：2008-01-01 00:00:00
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journal_title：Biological research
pub_type： 杂志文章
doi：10.4067/s0716-97602002000300012
更新日期：2002-01-01 00:00:00
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journal_title：Biological research
pub_type： 杂志文章
doi：10.1186/0717-6287-47-41
更新日期：2014-09-05 00:00:00
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journal_title：Biological research
pub_type： 杂志文章
doi：
更新日期：1992-01-01 00:00:00
abstract：BACKGROUND:Pancreatic ductal adenocarcinoma (PDAC) is highly aggressive with poor prognosis. Long non-coding RNAs (lncRNAs), a group of non-coding RNAs, play important roles in the progression of PDAC. This study aimed to investigate the potential involvement of lncRNA CCAT2 in PDAC tumorigenesis. METHODS:Expression o...
journal_title：Biological research
pub_type： 杂志文章
doi：10.1186/s40659-017-0149-0
更新日期：2018-01-03 00:00:00
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journal_title：Biological research
pub_type： 杂志文章
doi：10.4067/S0716-97602013000300003
更新日期：2013-01-01 00:00:00
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journal_title：Biological research
pub_type： 杂志文章
doi：
更新日期：1996-01-01 00:00:00
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pub_type： 杂志文章
doi：10.4067/s0716-97602005000200011
更新日期：2005-01-01 00:00:00
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journal_title：Biological research
pub_type： 杂志文章,评审
doi：
更新日期：1995-01-01 00:00:00
abstract：:A peptide from hindguts of the Triatoma infestans, the hematophagous Chagas' insect vector, activates adenylyl cyclase activity in Trypanosoma cruzi epimastigote membranes and stimulates the in vitro differentiation of epimastigotes (proliferative and non-infectious forms) to metacyclic trypomastigotes (non-proliferat...
journal_title：Biological research
pub_type： 杂志文章
doi：
更新日期：1993-01-01 00:00:00
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journal_title：Biological research
pub_type： 杂志文章
doi：/S0716-97602010000400008
更新日期：2010-01-01 00:00:00
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journal_title：Biological research
pub_type： 杂志文章
doi：
更新日期：1995-01-01 00:00:00
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journal_title：Biological research
pub_type： 杂志文章
doi：10.4067/s0716-97602007000100001
更新日期：2007-01-01 00:00:00
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journal_title：Biological research
pub_type： 杂志文章,评审
doi：
更新日期：1999-01-01 00:00:00
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journal_title：Biological research
pub_type： 杂志文章
doi：10.4067/s0716-97602004000200017
更新日期：2004-01-01 00:00:00
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journal_title：Biological research
pub_type： 杂志文章
doi：10.1186/0717-6287-47-40
更新日期：2014-09-04 00:00:00
abstract：BACKGROUND:Impaired wound healing is a complication of diabetes and a serious problem in clinical practice. We previously found that whey protein (WP) was able to regulate wound healing normally in streptozotocin (STZ)-diabetic models. This subsequent study was designed to assess the effect of WP on heat shock protein-...
journal_title：Biological research
pub_type： 杂志文章
doi：10.1186/s40659-015-0044-5
更新日期：2015-10-01 00:00:00
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journal_title：Biological research
pub_type： 杂志文章
doi：/S0716-97602011000400008
更新日期：2011-01-01 00:00:00
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journal_title：Biological research
pub_type： 杂志文章
doi：
更新日期：1994-01-01 00:00:00
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journal_title：Biological research
pub_type： 杂志文章
doi：10.4067/s0716-97602003000200015
更新日期：2003-01-01 00:00:00
abstract：:Santiago de Chile has a high level of air pollution with ozone (O3), carbon monoxide (CO) and particles equal or smaller than 10 microns (PM10) usually exceeding the accepted standards. This situation should be noxious for the exposed population and particularly--in the case of O3 and PM10--for the respiratory system....
journal_title：Biological research
pub_type： 杂志文章
doi：
更新日期：1998-01-01 00:00:00
abstract：:Vasopressin and bradykinin are two of the most important peptides in regulating vascular tone, water, and ionic balance in the body, and thus they play a key role in controlling blood pressure. In addition to being a potent vasoconstrictor, Vasopressin also has an antidiuretic activity in the kidney, whereas kinins re...
journal_title：Biological research
pub_type： 杂志文章,评审
doi：
更新日期：1999-01-01 00:00:00
