Abstract：
:The present work was intended to evaluate the preparation of antigens, as well as the production and characterization of anti cAMP and cGMP antibodies. Such antibodies were obtained from rabbits, and we used 2'O-succinyl cyclic nucleotide derivate, conjugated with human serum albumin, as antigen. The characterization of the antibodies was monitored by their immunoreactivity with the labelled antigen [125I]-cyclic nucleotide. This assay consists in a competition between a labelled and an unlabelled antigen for a fixed number of binding sites present in the specific antibody. The antibodies were specific for the inducing antigens. Cross-reactivity tests showed low degree competition between the immunogen and other antigens. The very high affinity, high quality and specificity of the generated antibodies indicate that they may be used not only in radioimmunoassay and immunocytochemistry methodologies, but also as bioblockers of physiological pathways.
journal_name
Biol Resjournal_title
Biological researchauthors
Defagot C,Zubin Psubject
Has Abstractpub_date
1994-01-01 00:00:00pages
193-7issue
3-4eissn
0716-9760issn
0717-6287journal_volume
27pub_type
杂志文章abstract：BACKGROUND:Prognosis remains one of most crucial determinants of gastric cancer (GC) treatment, but current methods do not predict prognosis accurately. Identification of additional biomarkers is urgently required to identify patients at risk of poor prognoses. METHODS:Tissue microarrays were used to measure expressio...
journal_title：Biological research
pub_type： 杂志文章
doi：10.1186/s40659-019-0249-0
更新日期：2019-08-09 00:00:00
abstract：BACKGROUND:Previous studies have shown that long noncoding RNA (lncRNA) LINC00483 was aberrantly expressed in human cancers, including gastric cancer. However, the regulatory mechanism of this lncRNA in gastric cancer remains largely unknown. The present study aimed to investigate the effect of LINC00483 on gastric can...
journal_title：Biological research
pub_type： 杂志文章
doi：10.1186/s40659-020-00283-6
更新日期：2020-04-15 00:00:00
abstract：:The species Heteropterys aphrodisiaca is commonly used as a stimulant by popular medicine in the Cerrado, a savanna-like biome, Brazil. Recent studies have proved its protective effects on testes of animals submitted to treatment using Cyclosporine A, as well as its stimulus effect in increasing testosterone secretion...
journal_title：Biological research
pub_type： 杂志文章
doi：/S0716-97602011000300004
更新日期：2011-01-01 00:00:00
abstract：:The development of plant transformation in the mid-1980s and of many new tools for cell biology, molecular genetics, and biochemistry has resulted in enormous progress in plant biology in the past decade. With the completion of the genome sequence of Arabidopsis thaliana just around the corner, we can expect even fast...
journal_title：Biological research
pub_type： 杂志文章,评审
doi：
更新日期：1999-01-01 00:00:00
abstract：:Caffeic acid, rosmarinic acid and oligomers of caffeic acid with multiple catechol groups are all constituents of Salvia officinalis. Their antioxidant potential was investigated with regard to their radical scavenging activity and the stability and structure of the intermediate radicals. Pulse-radiolytic studies reve...
journal_title：Biological research
pub_type： 杂志文章
doi：10.4067/s0716-97602004000200017
更新日期：2004-01-01 00:00:00
abstract：:Peroxisomes are thought to be formed by division of pre-existing peroxisomes after the import of newly synthesized proteins. However, it has been recently suggested that the endoplasmic reticulum (ER) provides an alternative de novo mechanism for peroxisome biogenesis in some cells. To test a possible role of the ER-G...
journal_title：Biological research
pub_type： 杂志文章
doi：10.4067/s0716-97602007000200014
更新日期：2007-01-01 00:00:00
abstract：:Most Colombian populations stem from the admixture of Caucasians, Amerindians and Negroids. In the world, these two latter ethnical groups show a significantly higher prevalence of epilepsy than the former one. We tested the hypothesis that the high prevalence of idiopathic epilepsy with generalized tonic clonic seizu...
journal_title：Biological research
pub_type： 杂志文章
doi：
更新日期：1996-01-01 00:00:00
abstract：:7-ketocholesterol (7-KC) differs from cholesterol by a functional ketone group at C7. It is an oxygenated cholesterol derivative (oxysterol), commonly present in oxidized low-density lipoprotein (LDL). Oxysterols are generated and participate in several physiologic and pathophysiologic processes. For instance, the cyt...
journal_title：Biological research
pub_type： 杂志文章
doi：/S0716-97602010000400008
更新日期：2010-01-01 00:00:00
abstract：:Skeletal muscles have the potential to regenerate by activation of quiescent satellite cells, however, the molecular signature that governs satellite cells during muscle regeneration is not well defined. Myosin light chains (Myls) are sarcomere-related proteins as traditional regulator of muscle contraction. In this r...
journal_title：Biological research
pub_type： 杂志文章
doi：/S0716-97602009000100013
更新日期：2009-01-01 00:00:00
abstract：BACKGROUND:Psoriasis is a common chronic inflammatory skin disease. Keratinocytes hyperproliferation and excessive inflammatory response contribute to psoriasis pathogenesis. The agents able to attenuate keratinocytes hyperproliferation and excessive inflammatory response are considered to be potentially useful for pso...
journal_title：Biological research
pub_type： 杂志文章
doi：10.1186/s40659-020-00316-0
更新日期：2020-10-20 00:00:00
abstract：:Oxidant/antioxidant imbalance has been reported in some infectious diseases, including community-acquired pneumonia (CAP). The aim was to assess the antioxidant status in adults with CAP and its relationship with clinical severity at admission. Fifty-nine patients with CAP were enrolled and categorized at admission by...
journal_title：Biological research
pub_type： 杂志文章
doi：10.4067/S0716-97602013000200013
更新日期：2013-01-01 00:00:00
abstract：:Calcium regulation of several transcription factors involves different calcium-dependent signaling cascades and engages cytoplasmic as well as nuclear calcium signals. The study of the specific sources of calcium signals involved in regulation of gene expression in skeletal muscle has been addressed only recently. In ...
journal_title：Biological research
pub_type： 杂志文章,评审
doi：10.4067/s0716-97602004000400028
更新日期：2004-01-01 00:00:00
abstract：BACKGROUND:Tear desiccation on a glass surface followed by transmitted-light microscopy has served as diagnostic test for dry eye. Four distinctive morphological domains (zones I, II, III and transition band) have been recently recognized in tear microdesiccates. Physicochemical dissimilarities among those domains hamp...
journal_title：Biological research
pub_type： 杂志文章
doi：10.1186/s40659-016-0089-0
更新日期：2016-06-03 00:00:00
abstract：:We review the development of our knowledge and interpretations of the intermediary metabolism of Trypanosoma (Schizotrypanum) cruzi. Already in the 1950's it was clearly established that when this organism was exposed to large external concentrations of carbohydrates it was unable to catabolize them completely, even i...
journal_title：Biological research
pub_type： 杂志文章,评审
doi：
更新日期：1993-01-01 00:00:00
abstract：BACKGROUND:The root of Angelica sinensis (AS), also known as "Dang-gui," was a popular herbal medicine widely used in the treatment of gynecological diseases in China, Korea, and Japan for a long time. This study aimed to determine the effects of ethyl acetate fraction from Angelica sinensis (EAAS) on the interleukin-1...
journal_title：Biological research
pub_type： 杂志文章
doi：10.1186/0717-6287-47-41
更新日期：2014-09-05 00:00:00
abstract：:A peptide from hindguts of the Triatoma infestans, the hematophagous Chagas' insect vector, activates adenylyl cyclase activity in Trypanosoma cruzi epimastigote membranes and stimulates the in vitro differentiation of epimastigotes (proliferative and non-infectious forms) to metacyclic trypomastigotes (non-proliferat...
journal_title：Biological research
pub_type： 杂志文章
doi：
更新日期：1993-01-01 00:00:00
abstract：:X-Ray diffraction is the only method currently available for the determination of the detailed three-dimensional structure of macromolecules. In this article, the principles behind the use of X-ray crystallography in the study of protein structure are presented. The results obtained in its application to several enzym...
journal_title：Biological research
pub_type： 杂志文章,评审
doi：
更新日期：1996-01-01 00:00:00
abstract：:The radiobiocomplexes labeled with technetium-99m (Tc-99m) have been widely used in nuclear medicine in single photon emission computed tomography and in basic research. The aim of this study was to assess the influence of a Nectandra membranacea extract on the bioavailability of the sodium pertechnetate (Na(99m)TcO(4...
journal_title：Biological research
pub_type： 杂志文章
doi：/S0716-97602007000200004
更新日期：2007-01-01 00:00:00
abstract：BACKGROUND:Melanoma took top position among the lethal cancers and, despite there have been some great attempts made to increase the natural life of patients with metastatic disease, long-lasting and complete remissions are few. Piceatannol, owns the similar function as resveratrol, has been defined as an anti-cancer a...
journal_title：Biological research
pub_type： 杂志文章
doi：10.1186/s40659-017-0141-8
更新日期：2017-10-17 00:00:00
abstract：:In mammals, neurogenesis continues during adulthood in restricted places of the nervous system, namely the subventricular zone, the dentate gyms and the olfactory epithelium. A dual role of the second messenger nitric oxide has been reported in such places, either promoting or inhibiting proliferation of neuronal prec...
journal_title：Biological research
pub_type： 杂志文章,评审
doi：10.4067/s0716-97602006000500002
更新日期：2006-01-01 00:00:00
abstract：:The aim of this study was to compare the effects of Mediterranean-type diet (MD), high-fat diet (HFD), and red wine supplementation on plasma concentration of emergent haemostatic cardiovascular risk factors (HCVRF) and on variables of primary haemostasis (bleeding time, plasma von Willebrand factor and platelet aggre...
journal_title：Biological research
pub_type： 临床试验,杂志文章
doi：10.4067/s0716-97602004000200007
更新日期：2004-01-01 00:00:00
abstract：:Bovine median eminence contains a factor difference from gonadotropin-releasing hormone (GnRH) than increases basal luteinizing hormone (LH) secretion and potentiates GnRH-stimulated LH release. We compared the effect of hypothalamic neuropeptides on basal and GnRH-stimulated LH secretion using rat pituitary cells und...
journal_title：Biological research
pub_type： 杂志文章
doi：
更新日期：1994-01-01 00:00:00
abstract：:The objective of this study was to test the hypothesis that root of maguey (Agave salmiana Otto ex Salm-Dyck) seedlings reacts during the first 24 h to low substrate water potential (PsiW), by anatomical modifications. Three-4 cm root length seedlings were planted in vermiculite for 24 h at PsiW between -0.03 and -2.3...
journal_title：Biological research
pub_type： 杂志文章
doi：/S0716-97602009000200013
更新日期：2009-01-01 00:00:00
abstract：:Hematopoietic stem cell transplantation is the accepted therapy of choice for a variety of malignant and non-malignant diseases in children and adults. Initially developed as rescue therapy for a patient with cancer after high doses of chemotherapy and radiation as well as the correction of severe deficiencies in the ...
journal_title：Biological research
pub_type： 历史文章,杂志文章
doi：10.4067/S0716-97602012000300012
更新日期：2012-01-01 00:00:00
abstract：BACKGROUND:IcarisideII (ICAII) could promote the differentiation of adipose tissue-derived stem cells (ADSCs) to Schwann cells (SCs), leading to improvement of erectile function (EF) and providing a realistic therapeutic option for the treatment of erectile dysfunction (ED). However, the underlying molecular mechanisms...
journal_title：Biological research
pub_type： 杂志文章
doi：10.1186/s40659-019-0262-3
更新日期：2019-10-03 00:00:00
abstract：:In the last few decades, several works on event related potentials (ERPs hereafter) during sleep have been reported. In spite of numerous studies, clear methodological rules for this kind of study are often missing, making it difficult to valorize the scope of these results. We propose here a description of methodolog...
journal_title：Biological research
pub_type： 杂志文章
doi：/S0716-97602008000300004
更新日期：2008-01-01 00:00:00
abstract：:The anti-inflammatory and redox protective effects of the citronellal (CT) were evaluated using in vivo and in vitro tests. Intraperitoneal (i.p.) administration of CT (50, 100, and 200 mg/kg) inhibited (p < 0.05) the carrageenan-induced leukocyte migration to the peritoneal cavity. Additionally, the carrageenan- and ...
journal_title：Biological research
pub_type： 杂志文章
doi：/S0716-97602011000400008
更新日期：2011-01-01 00:00:00
abstract：:Apoptosis is a means by which organisms dispose of unwanted cells without inducing an inflammatory response. Alterations in apoptosis is a common process by which cells become cancerous. Paradoxically, many cancer chemotherapeutics preferentially kill cancer cells by inducing apoptosis. Diacylglycerol is a lipid secon...
journal_title：Biological research
pub_type： 杂志文章,评审
doi：10.4067/s0716-97602002000200014
更新日期：2002-01-01 00:00:00
abstract：:After the progress made during the genomics era, bioinformatics was tasked with supporting the flow of information generated by nanobiotechnology efforts. This challenge requires adapting classical bioinformatic and computational chemistry tools to store, standardize, analyze, and visualize nanobiotechnological inform...
journal_title：Biological research
pub_type： 杂志文章
doi：10.4067/S0716-97602011000100006
更新日期：2011-01-01 00:00:00
abstract：:Eight Chilean strains of Dunaliella salina obtained within a restricted geographic range, but exhibiting a high variability in their morphology, rate of growth and carotenogenic capacity, were analyzed by Random Amplified Polymorphic DNA (RAPD-PCR) Twenty of the 50 random primers (D, P, OPA and OPD series) that were t...
journal_title：Biological research
pub_type： 杂志文章
doi：10.4067/s0716-97602001000100012
更新日期：2001-01-01 00:00:00
