Abstract：
:7-ketocholesterol (7-KC) differs from cholesterol by a functional ketone group at C7. It is an oxygenated cholesterol derivative (oxysterol), commonly present in oxidized low-density lipoprotein (LDL). Oxysterols are generated and participate in several physiologic and pathophysiologic processes. For instance, the cytotoxic effects of oxidized LDL have been widely attributed to bioactive compounds like oxysterols. The toxicity is in part due to 7-KC. Here we aimed to demonstrate the possibility of incorporating 7-KC into the synthetic nanoemulsion LDE, which resembles LDL in composition and behavior. This would provide a suitable artificial particle resembling LDL to study 7-KC metabolism. We were able to incorporate 7-KC in several amounts into LDE. The incorporation was evaluated and confirmed by several methods, including gel filtration chromatography, using radiolabeled lipids. The incorporation did not change the main lipid composition characteristics of the new nanoparticle. Particle sizes were also evaluated and did not differ from LDE. In vivo studies were performed by injecting the nanoemulsion into mice. The plasma kinetics and the targeted organs were the same as described for LDE. Therefore, 7-KC-LDE maintains composition, size and some functional characteristics of LDE and could be used in experiments dealing with 7-ketocholesterol metabolism in lipoproteins.
journal_name
Biol Resjournal_title
Biological researchauthors
Favero GM,Maranhão RC,Maria DA,Levy D,Bydlowski SPdoi
/S0716-97602010000400008subject
Has Abstractpub_date
2010-01-01 00:00:00pages
439-44issue
4eissn
0716-9760issn
0717-6287pii
S0716-97602010000400008journal_volume
43pub_type
杂志文章abstract：:Breast cancer is the most common malignancy among women. Chilean studies reveal that this cancer presents the third highest mortality rate. A family history of breast cancer is one of the major risk factors for the development of this disease. BRCA1 and BRCA2 are the two main hereditary breast cancer susceptibility ge...
journal_title：Biological research
pub_type： 杂志文章
doi：10.4067/s0716-97602002000100011
更新日期：2002-01-01 00:00:00
abstract：:A peptide from hindguts of the Triatoma infestans, the hematophagous Chagas' insect vector, activates adenylyl cyclase activity in Trypanosoma cruzi epimastigote membranes and stimulates the in vitro differentiation of epimastigotes (proliferative and non-infectious forms) to metacyclic trypomastigotes (non-proliferat...
journal_title：Biological research
pub_type： 杂志文章
doi：
更新日期：1993-01-01 00:00:00
abstract：:The present work was intended to evaluate the preparation of antigens, as well as the production and characterization of anti cAMP and cGMP antibodies. Such antibodies were obtained from rabbits, and we used 2'O-succinyl cyclic nucleotide derivate, conjugated with human serum albumin, as antigen. The characterization ...
journal_title：Biological research
pub_type： 杂志文章
doi：
更新日期：1994-01-01 00:00:00
abstract：:The anti-inflammatory and redox protective effects of the citronellal (CT) were evaluated using in vivo and in vitro tests. Intraperitoneal (i.p.) administration of CT (50, 100, and 200 mg/kg) inhibited (p < 0.05) the carrageenan-induced leukocyte migration to the peritoneal cavity. Additionally, the carrageenan- and ...
journal_title：Biological research
pub_type： 杂志文章
doi：/S0716-97602011000400008
更新日期：2011-01-01 00:00:00
abstract：:Thymulin is a well-characterized thymic hormone that exists as a nonapeptide coupled to equimolar amounts of Zn2+. Thymulin is known to have multiple biological roles, including T cell differentiation, immune regulation, and analgesic functions. It has been shown that thymulin is produced by the reticulo-epithelial ce...
journal_title：Biological research
pub_type： 杂志文章
doi：/S0716-97602010000300005
更新日期：2010-01-01 00:00:00
abstract：:This study examined the effects of estrogen supplementation on markers of neutrophil infiltration and damage in skeletal muscle of rats following ischemia. Male and female gonad-intact rats, with or without 14 days of estrogen supplementation were subjected to two hours of hind-limb ischemia and sacrificed at 24, 48 o...
journal_title：Biological research
pub_type： 杂志文章
doi：10.4067/s0716-97602005000200011
更新日期：2005-01-01 00:00:00
abstract：BACKGROUND:This study aimed to determine the seasonal changes of total antioxidant activity and phenolic compounds in samples taken from leaves (April, July, October) and stems (April, July, October, January) of some almond (Prunus amygdalus L.) varieties (Nonpareil, Ferragnes and Texas). RESULTS:It was indicated that...
journal_title：Biological research
pub_type： 杂志文章
doi：10.1186/0717-6287-47-9
更新日期：2014-04-01 00:00:00
abstract：:The aim of this study is to describe the ultrastructural changes of the egg apparatus associated with fertilisation of the natural tetraploid Trifolium pratense. The pollen tube enters one of the synergids through the filliform apparatus from the micropyle. Before the entry of the pollen tube into the embryo sac, one ...
journal_title：Biological research
pub_type： 杂志文章
doi：/S0716-97602009000100003
更新日期：2009-01-01 00:00:00
abstract：BACKGROUND:The Tridax procumbens flavonoids (TPF), are well known for their medicinal properties among local natives. The TPF are traditionally used for dropsy, anaemia, arthritis, gout, asthma, ulcer, piles, and urinary problems. It also used in treating gastric problems, body pain, and rheumatic pains of joints. The ...
journal_title：Biological research
pub_type： 杂志文章
doi：10.1186/s40659-015-0043-6
更新日期：2015-09-12 00:00:00
abstract：:Twelve biological variables were submitted to dimensional analysis in accordance with the MLT-system of physics (M, mass; L, length; T, time). Each of these variables has a characteristic numerical value for the exponents alpha for mass, beta for length, and gamma for time. By means of Newton's reduction coefficient (...
journal_title：Biological research
pub_type： 杂志文章
doi：
更新日期：1992-01-01 00:00:00
abstract：:Exposure to arsenic via drinking water is considered as a worldwide problem. Studies have shown that arsenic exposure during pregnancy affects embryogenesis and offspring development in rats and mice. Zinc as a micronutrient regulates many physiological functions, including an antioxidative role under various toxic co...
journal_title：Biological research
pub_type： 杂志文章
doi：10.4067/S0716-97602013000200003
更新日期：2013-01-01 00:00:00
abstract：:Peroxisomes are thought to be formed by division of pre-existing peroxisomes after the import of newly synthesized proteins. However, it has been recently suggested that the endoplasmic reticulum (ER) provides an alternative de novo mechanism for peroxisome biogenesis in some cells. To test a possible role of the ER-G...
journal_title：Biological research
pub_type： 杂志文章
doi：10.4067/s0716-97602007000200014
更新日期：2007-01-01 00:00:00
abstract：:The expression of the acetyl xylan esterase II (axeII) gene from Penicillium purpurogenum is repressed by glucose and induced by xylan, as well as to a small degree by xylose and xylitol. This gene is expressed at neutral pH, but not under alkaline or acidic conditions, in agreement with previous findings for other xy...
journal_title：Biological research
pub_type： 杂志文章
doi：10.4067/s0716-97602004000100011
更新日期：2004-01-01 00:00:00
abstract：:Brain cells have a highly active oxidative metabolism, yet they contain only low to moderate superoxide dismutase and catalase activities. Thus, their antioxidant defenses rely mainly on cellular reduced glutathione levels. In this work, in cortical neurons we characterized viability and changes in reduced and oxidize...
journal_title：Biological research
pub_type： 杂志文章
doi：10.4067/s0716-97602006000100012
更新日期：2006-01-01 00:00:00
abstract：BACKGROUND:Understanding the molecular basis underlying the formation of bone-forming osteocytes and lipid-storing adipocytes will help provide insights into the cause of disorders originating in stem/progenitor cells and develop therapeutic treatments for bone- or adipose-related diseases. In this study, the role of R...
journal_title：Biological research
pub_type： 杂志文章
doi：10.1186/s40659-017-0148-1
更新日期：2017-12-26 00:00:00
abstract：:Despite the fact that the concept of sheet-flow in the pulmonary microcirculation of mammals was introduced more than three decades ago, the capillary circulatory model still prevails in the physiological literature. Since cardiac output is identical in the systemic and in pulmonary circulations, it is noteworthy that...
journal_title：Biological research
pub_type： 杂志文章
doi：10.4067/s0716-97602005000100007
更新日期：2005-01-01 00:00:00
abstract：:Cyanobacteria display a large diversity of cellular forms ranging from unicellular to complex multicellular filaments or aggregates. Species in the group present a wide range of metabolic characteristics including the fixation of atmospheric nitrogen, resistance to extreme environments, production of hydrogen, seconda...
journal_title：Biological research
pub_type： 杂志文章,评审
doi：10.4067/S0716-97602013000400009
更新日期：2013-01-01 00:00:00
abstract：:Unilateral brain damage which includes the hippocampus leads to memory impairments consistent with hemispheric specialization on the same side. Damage to the corpus callosum, the major connecting pathway between the left and right hemispheres, also leads to memory impairments. This suggests both hemispheric specializa...
journal_title：Biological research
pub_type： 杂志文章,评审
doi：
更新日期：1995-01-01 00:00:00
abstract：BACKGROUND:Left ventricular diastolic dysfunction is one of the main characteristics of heart failure patients with a preserved left ventricular ejection fraction. As bilirubin is regarded as an important endogenous antioxidant molecule, serum total bilirubin levels were compared between heart failure patients with a p...
journal_title：Biological research
pub_type： 杂志文章
doi：10.1186/0717-6287-47-7
更新日期：2014-03-26 00:00:00
abstract：:Although several histone variants have been studied in both animal and plant organisms, little is known about their distribution during processes that involve alterations in chromatin function, such as differentiation, dedifferentiation and hormone treatment. In this study we evaluated the ratio of each histone varian...
journal_title：Biological research
pub_type： 杂志文章
doi：/S0716-97602009000400006
更新日期：2009-01-01 00:00:00
abstract：:Control of smooth muscle is vital for health. The major route to contraction is a rise in intracellular [Ca2+], determined by the entry and efflux of Ca2+ and release and re-uptake into the sarcoplasmic reticulum (SR). We review these processes in myometrium, to better understand excitation-contraction coupling and de...
journal_title：Biological research
pub_type： 杂志文章
doi：10.4067/s0716-97602004000400017
更新日期：2004-01-01 00:00:00
abstract：:Guanine nucleotide binding proteins (GTP-binding proteins) function as transducers of signals in different cellular processes. We have identified several GTP-binding proteins in Trypanosoma cruzi by Western blot analyses. Six polypeptide bands, p20, p25, p28, p31, p37 and p38, were specifically detected in epimastigot...
journal_title：Biological research
pub_type： 杂志文章
doi：
更新日期：1993-01-01 00:00:00
abstract：BACKGROUND:Despite manifold benefits of nanoparticles (NPs), less information on the risks of NPs to human health and environment has been studied. Cobalt oxide nanoparticles (Co3O4-NPs) have been reported to cause toxicity in several organisms. In this study, we have investigated the role of Co3O4-NPs in inducing phyt...
journal_title：Biological research
pub_type： 杂志文章
doi：10.1186/s40659-016-0080-9
更新日期：2016-03-18 00:00:00
abstract：:Oxidant/antioxidant imbalance has been reported in some infectious diseases, including community-acquired pneumonia (CAP). The aim was to assess the antioxidant status in adults with CAP and its relationship with clinical severity at admission. Fifty-nine patients with CAP were enrolled and categorized at admission by...
journal_title：Biological research
pub_type： 杂志文章
doi：10.4067/S0716-97602013000200013
更新日期：2013-01-01 00:00:00
abstract：:Bovine median eminence contains a factor difference from gonadotropin-releasing hormone (GnRH) than increases basal luteinizing hormone (LH) secretion and potentiates GnRH-stimulated LH release. We compared the effect of hypothalamic neuropeptides on basal and GnRH-stimulated LH secretion using rat pituitary cells und...
journal_title：Biological research
pub_type： 杂志文章
doi：
更新日期：1994-01-01 00:00:00
abstract：BACKGROUND:Asthma is a complex disease influenced by multiple genetic and environmental factors. While Madeira has the highest prevalence of asthma in Portugal (14.6%), the effect of both genetic and environmental factors in this population has never been assessed. We categorized 98 asthma patients according to the Glo...
journal_title：Biological research
pub_type： 杂志文章
doi：10.1186/0717-6287-47-40
更新日期：2014-09-04 00:00:00
abstract：BACKGROUND:Psoralen is a coumarin-like and coumarin-related benzofuran glycoside, which is a commonly used traditional Chinese medicine to treat patients with kidney and spleen-yang deficiency symptom. Psoralen has been reported to show estrogen-like activity, antioxidant activity, osteoblastic proliferation accelerati...
journal_title：Biological research
pub_type： 杂志文章
doi：10.1186/s40659-019-0241-8
更新日期：2019-07-05 00:00:00
abstract：:In mammals, neurogenesis continues during adulthood in restricted places of the nervous system, namely the subventricular zone, the dentate gyms and the olfactory epithelium. A dual role of the second messenger nitric oxide has been reported in such places, either promoting or inhibiting proliferation of neuronal prec...
journal_title：Biological research
pub_type： 杂志文章,评审
doi：10.4067/s0716-97602006000500002
更新日期：2006-01-01 00:00:00
abstract：:The radiobiocomplexes labeled with technetium-99m (Tc-99m) have been widely used in nuclear medicine in single photon emission computed tomography and in basic research. The aim of this study was to assess the influence of a Nectandra membranacea extract on the bioavailability of the sodium pertechnetate (Na(99m)TcO(4...
journal_title：Biological research
pub_type： 杂志文章
doi：/S0716-97602007000200004
更新日期：2007-01-01 00:00:00
abstract：:Santiago de Chile has a high level of air pollution with ozone (O3), carbon monoxide (CO) and particles equal or smaller than 10 microns (PM10) usually exceeding the accepted standards. This situation should be noxious for the exposed population and particularly--in the case of O3 and PM10--for the respiratory system....
journal_title：Biological research
pub_type： 杂志文章
doi：
更新日期：1998-01-01 00:00:00
