Abstract：
:Transducin serves as a mediator between the receptor protein, rhodopsin, and the effector protein, cGMP phosphodiesterase, in the visual process. Transducin is a protein composed of three polypeptides: T alpha, T beta, and T gamma, and acts as two functional units, the alpha-subunit and the beta gamma-complex. In the present study, I describe an efficient and fast method of purifying T alpha and T beta gamma using chromatography on a blue agarose column connected in tandem with an omega-amino octylagarose column. The recombination of T alpha and T beta gamma reconstitutes the functional heterotrimeric holoprotein, as demonstrated by the recovery of three native properties of transducin: 1) its capacity to exchange guanine nucleotide, 2) its GTP hydrolytic activity, and 3) the ADP-ribosylation of T alpha catalysed by pertussis toxin.
journal_name
Biol Resjournal_title
Biological researchauthors
Bubis Jsubject
Has Abstractpub_date
1995-01-01 00:00:00pages
291-9issue
4eissn
0716-9760issn
0717-6287journal_volume
28pub_type
杂志文章abstract：:The objective of this study was to test the hypothesis that root of maguey (Agave salmiana Otto ex Salm-Dyck) seedlings reacts during the first 24 h to low substrate water potential (PsiW), by anatomical modifications. Three-4 cm root length seedlings were planted in vermiculite for 24 h at PsiW between -0.03 and -2.3...
journal_title：Biological research
pub_type： 杂志文章
doi：/S0716-97602009000200013
更新日期：2009-01-01 00:00:00
abstract：:7-ketocholesterol (7-KC) differs from cholesterol by a functional ketone group at C7. It is an oxygenated cholesterol derivative (oxysterol), commonly present in oxidized low-density lipoprotein (LDL). Oxysterols are generated and participate in several physiologic and pathophysiologic processes. For instance, the cyt...
journal_title：Biological research
pub_type： 杂志文章
doi：/S0716-97602010000400008
更新日期：2010-01-01 00:00:00
abstract：:The protein nucleolin, functionally involved in the main steps of ribosome biogenesis, is codified by a single copy gene in mammals. Here we report that at least three different genes codify for this protein in carp fish (Cyprinus carpio). This is the first description of the genomic organization of nucleolin in a tel...
journal_title：Biological research
pub_type： 杂志文章
doi：10.4067/s0716-97602006000200017
更新日期：2006-01-01 00:00:00
abstract：:The aim of this study was to compare the effects of Mediterranean-type diet (MD), high-fat diet (HFD), and red wine supplementation on plasma concentration of emergent haemostatic cardiovascular risk factors (HCVRF) and on variables of primary haemostasis (bleeding time, plasma von Willebrand factor and platelet aggre...
journal_title：Biological research
pub_type： 临床试验,杂志文章
doi：10.4067/s0716-97602004000200007
更新日期：2004-01-01 00:00:00
abstract：BACKGROUND:Papillary thyroid cancer (PTC) is the most common malignancy of all thyroid cancers. LncRNA LINC00460 has been proved to play roles in the oncogenesis and progression of various tumors, including papillary thyroid cancer. However, the potential molecular mechanism of LINC00460 in PTC is poorly investigated. ...
journal_title：Biological research
pub_type： 杂志文章
doi：10.1186/s40659-019-0269-9
更新日期：2019-12-23 00:00:00
abstract：:Invasive properties of 15 strains of thermotolerant Campylobacter (12 C. jejuni and 3 C. coli) were studied using HeLa cells cultures. In four of them (3 C. jejuni and 1 C. coli), randomly selected, intestinal perfusion experiments were conducted in order to asses enterotoxigenicity. All strains were able to invade He...
journal_title：Biological research
pub_type： 杂志文章
doi：
更新日期：1995-01-01 00:00:00
abstract：BACKGROUND:Limonoids are highly oxygenated compounds with a prototypical structure. Their occurrence in the plant kingdom is mainly confined to plant families of Meliaceae and Rutaceae. Owing to their wide range of pharmacological and therapeutic properties, this study was aimed at investigating the potential nitric ox...
journal_title：Biological research
pub_type： 杂志文章
doi：10.1186/s40659-015-0049-0
更新日期：2015-10-13 00:00:00
abstract：:Exposure to arsenic via drinking water is considered as a worldwide problem. Studies have shown that arsenic exposure during pregnancy affects embryogenesis and offspring development in rats and mice. Zinc as a micronutrient regulates many physiological functions, including an antioxidative role under various toxic co...
journal_title：Biological research
pub_type： 杂志文章
doi：10.4067/S0716-97602013000200003
更新日期：2013-01-01 00:00:00
abstract：BACKGROUND:Pancreatic ductal adenocarcinoma (PDAC) is highly aggressive with poor prognosis. Long non-coding RNAs (lncRNAs), a group of non-coding RNAs, play important roles in the progression of PDAC. This study aimed to investigate the potential involvement of lncRNA CCAT2 in PDAC tumorigenesis. METHODS:Expression o...
journal_title：Biological research
pub_type： 杂志文章
doi：10.1186/s40659-017-0149-0
更新日期：2018-01-03 00:00:00
abstract：:Loss of Imprinting (LOI) of IGF2 and over-expressed IGF2 are associated with tumorigenesis. Our previous epidemiological study found a relatively high frequency of IGF2 LOI in healthy mid-gestation pregnant women. The aim of this study is to determine whether the expression of IGF2 is associated with its imprinting st...
journal_title：Biological research
pub_type： 杂志文章
doi：10.4067/S0716-97602012000400004
更新日期：2012-01-01 00:00:00
abstract：:In the last few decades, several works on event related potentials (ERPs hereafter) during sleep have been reported. In spite of numerous studies, clear methodological rules for this kind of study are often missing, making it difficult to valorize the scope of these results. We propose here a description of methodolog...
journal_title：Biological research
pub_type： 杂志文章
doi：/S0716-97602008000300004
更新日期：2008-01-01 00:00:00
abstract：:The hypothesis that the vacation-study-expectancy scholar regime produces most of the monthly rhythm of the age at menarche (AaM) was tested. Studies on monthly menarche incidence (MI) refuted climatic factors as a main factor in this rhythm, and indicated that the main factor of this rhythm is the succession of expec...
journal_title：Biological research
pub_type： 杂志文章,评审
doi：10.4067/S0716-97602013000100003
更新日期：2013-01-01 00:00:00
abstract：:Bovine median eminence contains a factor difference from gonadotropin-releasing hormone (GnRH) than increases basal luteinizing hormone (LH) secretion and potentiates GnRH-stimulated LH release. We compared the effect of hypothalamic neuropeptides on basal and GnRH-stimulated LH secretion using rat pituitary cells und...
journal_title：Biological research
pub_type： 杂志文章
doi：
更新日期：1994-01-01 00:00:00
abstract：BACKGROUND:Zinc finger RNA binding protein (ZFR) is involved in the regulation of growth and cancer development. However, little is known about ZFR function in pancreatic cancer. METHODS:Herein, to investigate whether ZFR is involved in tumor growth, Oncomine microarray data was firstly used to evaluate ZFR gene expre...
journal_title：Biological research
pub_type： 杂志文章
doi：10.1186/s40659-016-0086-3
更新日期：2016-05-13 00:00:00
abstract：:Allium cepa L. meristems were used as a plant model to study the p53-independent control of S and G2 phases by checkpoint pathways, in eukaryotic cells. Checkpoint blocks were induced at early and mid S by hydroxyurea. After their spontaneous override, cells became accumulated in G2-prophase, giving rise later on to a...
journal_title：Biological research
pub_type： 杂志文章
doi：10.4067/s0716-97602003000300009
更新日期：2003-01-01 00:00:00
abstract：:Bilateral aspiration lesions of the fimbria-fornix were performed in 10 male Sprague Dawley rats weighing 240-300 g under chloral hydrate narcose (420 mg/kg). Another 9 animals were operated in the same way, but no aspiration was carried out to constitute a control group. A week after surgery recording and stimulation...
journal_title：Biological research
pub_type： 杂志文章
doi：
更新日期：1996-01-01 00:00:00
abstract：:Calcium regulation of several transcription factors involves different calcium-dependent signaling cascades and engages cytoplasmic as well as nuclear calcium signals. The study of the specific sources of calcium signals involved in regulation of gene expression in skeletal muscle has been addressed only recently. In ...
journal_title：Biological research
pub_type： 杂志文章,评审
doi：10.4067/s0716-97602004000400028
更新日期：2004-01-01 00:00:00
abstract：:The regeneration of natural tetraploid T. pratense, originated from Erzurum-Turkey, is reported in this study. This plant has low seed setting and hard seed problems due to polyploidy. Hypocotyl, cotyledon, apical meristems, epicotyl and young primary leaves were inoculated on MS and PC-L2 media containing different c...
journal_title：Biological research
pub_type： 杂志文章
doi：/S0716-97602008000100004
更新日期：2008-01-01 00:00:00
abstract：:Unilateral brain damage which includes the hippocampus leads to memory impairments consistent with hemispheric specialization on the same side. Damage to the corpus callosum, the major connecting pathway between the left and right hemispheres, also leads to memory impairments. This suggests both hemispheric specializa...
journal_title：Biological research
pub_type： 杂志文章,评审
doi：
更新日期：1995-01-01 00:00:00
abstract：:By using the fluorescent Ca2+ indicator fura 2, submicromolar levels of intracellular Ca2+ have been detected in Trypanosoma cruzi different stages. The intracellular transport mechanisms involved in maintaining Ca2+ homeostasis in T. cruzi have been characterized by measuring Ca2+ transport in digitonin-permeabilized...
journal_title：Biological research
pub_type： 杂志文章
doi：
更新日期：1993-01-01 00:00:00
abstract：:Hearing loss is the most common inherited sensorial deficiency in humans; about 1 in 1000 children suffer from severe or profound hearing loss at birth. Mutations in the GJB2 gene are the most common cause of prelingual, non-syndromic autosomal recessive deafness in many populations; the c.35delG mutation is the most ...
journal_title：Biological research
pub_type： 杂志文章
doi：10.4067/S0716-97602013000300003
更新日期：2013-01-01 00:00:00
abstract：:Hyptidendron canum (Pohl ex Benth.) Harley is a native tree of the Brazilian Savannah. The fish Oreochromis niloticus L. was used as an experimental model to determine the bioactivity of the crude ethanol extract as well as ethyl acetate, hexanic and chloroform fractions obtained from its leaves. The plant ethanol ext...
journal_title：Biological research
pub_type： 杂志文章
doi：/S0716-97602009000200003
更新日期：2009-01-01 00:00:00
abstract：:Prolactin (PRL) is a pituitary hormone that plays important roles in mammalian reproductive physiology, specially lactation. The regulation of PRL secretion shows important species differences. To study PRL regulation in a subhuman primate, the Cebus apella, we developed an heterologous radioimmunoassay using an antib...
journal_title：Biological research
pub_type： 杂志文章
doi：
更新日期：1997-01-01 00:00:00
abstract：:Many motor skills, once acquired, are stored over a long time period, probably sustained by permanent neuronal changes. Thus, in this paper we have investigated with quantitative stereology the generation and persistence of neuronal density changes in primary motor cortex (MI) following motor skill learning (skilled r...
journal_title：Biological research
pub_type： 杂志文章
doi：/S0716-97602008000400005
更新日期：2008-01-01 00:00:00
abstract：:Twelve biological variables were submitted to dimensional analysis in accordance with the MLT-system of physics (M, mass; L, length; T, time). Each of these variables has a characteristic numerical value for the exponents alpha for mass, beta for length, and gamma for time. By means of Newton's reduction coefficient (...
journal_title：Biological research
pub_type： 杂志文章
doi：
更新日期：1992-01-01 00:00:00
abstract：:Duchenne muscular dystrophy (DMD) is secondary to loss-of-function mutations in the dystrophin gene. The causes underlying the progression of DMD, differential muscle involvement, and the discrepancies in phenotypes among species with the same genetic defect are not understood. The mdx mouse, an animal model with dyst...
journal_title：Biological research
pub_type： 杂志文章,评审
doi：10.4067/s0716-97602005000400010
更新日期：2005-01-01 00:00:00
abstract：:Oxidative stress occurs early in the progression of Alzheimer disease, significantly before the development of the pathologic hallmarks, neurofibrillary tangles and senile plaques. In the first stage of development of the disease, amyloid-beta deposition and hyperphosphorylated tau function as compensatory responses a...
journal_title：Biological research
pub_type： 杂志文章,评审
doi：10.4067/s0716-97602006000100002
更新日期：2006-01-01 00:00:00
abstract：BACKGROUND:Prunella vulgaris L. is a medical plant cultivated in sloping, sun-shaded areas in China. Recently, owing to air-environmental stress, especially drought stress strongly inhibits plant growth and development, the appropriate fertilizer supply can alleviate these effects. However, these is little information ...
journal_title：Biological research
pub_type： 杂志文章
doi：10.1186/s40659-016-0069-4
更新日期：2016-02-24 00:00:00
abstract：BACKGROUND:This study aimed to determine the seasonal changes of total antioxidant activity and phenolic compounds in samples taken from leaves (April, July, October) and stems (April, July, October, January) of some almond (Prunus amygdalus L.) varieties (Nonpareil, Ferragnes and Texas). RESULTS:It was indicated that...
journal_title：Biological research
pub_type： 杂志文章
doi：10.1186/0717-6287-47-9
更新日期：2014-04-01 00:00:00
abstract：BACKGROUND:Tear desiccation on a glass surface followed by transmitted-light microscopy has served as diagnostic test for dry eye. Four distinctive morphological domains (zones I, II, III and transition band) have been recently recognized in tear microdesiccates. Physicochemical dissimilarities among those domains hamp...
journal_title：Biological research
pub_type： 杂志文章
doi：10.1186/s40659-016-0089-0
更新日期：2016-06-03 00:00:00
