Abstract：
BACKGROUND:miR-214 was demonstrated to be upregulated in models of renal disease and promoted fibrosis in renal injury independent of TGF-β signaling in vivo. However, the detailed role of miR-214 in acute kidney injury (AKI) and its underlying mechanism are still largely unknown. METHODS:In this study, an I/R-induced rat AKI model and a hypoxia-induced NRK-52E cell model were used to study AKI. The concentrations of kidney injury markers serum creatinine, blood urea nitrogen, and kidney injury molecule-1 were measured. The expressions of miR-214, tumor necrosis factor-α, interleukin (IL)-1β, IL-6, were detected by RT-qPCR. The protein levels of Bcl-2, Bax, Dickkopf-related protein 3, β-catenin, c-myc, and cyclinD1 were determined by western blot. Cell apoptosis and caspase 3 activity were evaluated by flow cytometry analysis and caspase 3 activity assay, respectively. Luciferase reporter assay was used to confirm the interaction between miR-214 and Dkk3. RESULTS:miR-214 expression was induced in ischemia-reperfusion (I/R)-induced AKI rat and hypoxic incubation of NRK-52E cells. Overexpression of miR-214 alleviated hypoxia-induced NRK-52E cell apoptosis while inhibition of miR-214 expression exerted the opposite effect. Dkk3 was identified as a target of miR-214. Anti-miR-214 abolished the inhibitory effects of DKK3 knockdown on hypoxia-induced NRK-52E cell apoptosis by inactivation of Wnt/β-catenin signaling. Moreover, miR-214 ameliorated AKI in vivo by inhibiting apoptosis and fibrosis through targeting Dkk3 and activating Wnt/β-catenin pathway. CONCLUSION:miR-214 ameliorates AKI by inhibiting apoptosis through targeting Dkk3 and activating Wnt/β-catenin signaling pathway, offering the possibility of miR-214 in the therapy of ischemic AKI.
journal_name
Biol Resjournal_title
Biological researchauthors
Zhu X,Li W,Li Hdoi
10.1186/s40659-018-0179-2subject
Has Abstractpub_date
2018-09-04 00:00:00pages
31issue
1eissn
0716-9760issn
0717-6287pii
10.1186/s40659-018-0179-2journal_volume
51pub_type
杂志文章abstract：:Despite the fact that the concept of sheet-flow in the pulmonary microcirculation of mammals was introduced more than three decades ago, the capillary circulatory model still prevails in the physiological literature. Since cardiac output is identical in the systemic and in pulmonary circulations, it is noteworthy that...
journal_title：Biological research
pub_type： 杂志文章
doi：10.4067/s0716-97602005000100007
更新日期：2005-01-01 00:00:00
abstract：:Cyanobacteria display a large diversity of cellular forms ranging from unicellular to complex multicellular filaments or aggregates. Species in the group present a wide range of metabolic characteristics including the fixation of atmospheric nitrogen, resistance to extreme environments, production of hydrogen, seconda...
journal_title：Biological research
pub_type： 杂志文章,评审
doi：10.4067/S0716-97602013000400009
更新日期：2013-01-01 00:00:00
abstract：:The anti-tumor effect of R-Phycoerythrin (R-PE) from Porphyra haitanensis was studied using cell line HeLa as an in vitro model and Sarcoma-180 (S180) tumor-bearing mice as an in vivo model. The results showed that the combination treatment of R-PE and photodynamic therapy PDT) significantly inhibited the growth of He...
journal_title：Biological research
pub_type： 杂志文章,评审
doi：10.4067/S0716-97602013000100013
更新日期：2013-01-01 00:00:00
abstract：:7-ketocholesterol (7-KC) differs from cholesterol by a functional ketone group at C7. It is an oxygenated cholesterol derivative (oxysterol), commonly present in oxidized low-density lipoprotein (LDL). Oxysterols are generated and participate in several physiologic and pathophysiologic processes. For instance, the cyt...
journal_title：Biological research
pub_type： 杂志文章
doi：/S0716-97602010000400008
更新日期：2010-01-01 00:00:00
abstract：:Santiago de Chile has a high level of air pollution with ozone (O3), carbon monoxide (CO) and particles equal or smaller than 10 microns (PM10) usually exceeding the accepted standards. This situation should be noxious for the exposed population and particularly--in the case of O3 and PM10--for the respiratory system....
journal_title：Biological research
pub_type： 杂志文章
doi：
更新日期：1998-01-01 00:00:00
abstract：:In the last twenty years an important effort in brain sciences, especially in cognitive science, has been the development of mathematical tool that can deal with the complexity of extensive recordings corresponding to the neuronal activity obtained from hundreds of neurons. We discuss here along with some historical i...
journal_title：Biological research
pub_type： 杂志文章,评审
doi：/S0716-97602007000500009
更新日期：2007-01-01 00:00:00
abstract：:As regards their morphology and biology, tumours consist of heterogeneous cell populations. The cancer stem cell (CSC) hypothesis assumes that a tumour is hierarchically organized and not all of the cells are equally capable of generating descendants, similarly to normal tissue. The only cells being able to self-renew...
journal_title：Biological research
pub_type： 杂志文章,评审
doi：10.1186/0717-6287-47-66
更新日期：2014-12-10 00:00:00
abstract：:The present work was intended to evaluate the preparation of antigens, as well as the production and characterization of anti cAMP and cGMP antibodies. Such antibodies were obtained from rabbits, and we used 2'O-succinyl cyclic nucleotide derivate, conjugated with human serum albumin, as antigen. The characterization ...
journal_title：Biological research
pub_type： 杂志文章
doi：
更新日期：1994-01-01 00:00:00
abstract：:Hematopoietic stem cell transplantation is the accepted therapy of choice for a variety of malignant and non-malignant diseases in children and adults. Initially developed as rescue therapy for a patient with cancer after high doses of chemotherapy and radiation as well as the correction of severe deficiencies in the ...
journal_title：Biological research
pub_type： 历史文章,杂志文章
doi：10.4067/S0716-97602012000300012
更新日期：2012-01-01 00:00:00
abstract：:Apoptosis is a means by which organisms dispose of unwanted cells without inducing an inflammatory response. Alterations in apoptosis is a common process by which cells become cancerous. Paradoxically, many cancer chemotherapeutics preferentially kill cancer cells by inducing apoptosis. Diacylglycerol is a lipid secon...
journal_title：Biological research
pub_type： 杂志文章,评审
doi：10.4067/s0716-97602002000200014
更新日期：2002-01-01 00:00:00
abstract：:The radiobiocomplexes labeled with technetium-99m (Tc-99m) have been widely used in nuclear medicine in single photon emission computed tomography and in basic research. The aim of this study was to assess the influence of a Nectandra membranacea extract on the bioavailability of the sodium pertechnetate (Na(99m)TcO(4...
journal_title：Biological research
pub_type： 杂志文章
doi：/S0716-97602007000200004
更新日期：2007-01-01 00:00:00
abstract：:The historical, lexical and conceptual issues embedded in stem cell biology are reviewed from technical, ethical, philosophical, judicial, clinical, economic and biopolitical perspectives. The mechanisms assigning the simultaneous capacity to self-renew and to differentiate to stem cells (immortal template DNA and asy...
journal_title：Biological research
pub_type： 历史文章,杂志文章,评审
doi：10.4067/S0716-97602012000300002
更新日期：2012-01-01 00:00:00
abstract：:Twelve biological variables were submitted to dimensional analysis in accordance with the MLT-system of physics (M, mass; L, length; T, time). Each of these variables has a characteristic numerical value for the exponents alpha for mass, beta for length, and gamma for time. By means of Newton's reduction coefficient (...
journal_title：Biological research
pub_type： 杂志文章
doi：
更新日期：1992-01-01 00:00:00
abstract：:X-Ray diffraction is the only method currently available for the determination of the detailed three-dimensional structure of macromolecules. In this article, the principles behind the use of X-ray crystallography in the study of protein structure are presented. The results obtained in its application to several enzym...
journal_title：Biological research
pub_type： 杂志文章,评审
doi：
更新日期：1996-01-01 00:00:00
abstract：:The neuropeptide oxytocin (OXT) is a key factor in the initiation and regulation of sociosexual behavior. The present study analyzes the effects of cohabitation and social challenge on plasma OXT concentration rates in guinea pig pairs in relation to male sociosexual behavior. The cohabitation phase lasted 3 days. On ...
journal_title：Biological research
pub_type： 杂志文章
doi：10.4067/s0716-97602006000200007
更新日期：2006-01-01 00:00:00
abstract：:Treatment of tumor-bearing mice with LD12.5 values of iodoacetate; IAA (1.84 mg/100 g b.w.) and/or dimethylsulphoxide; DMSO (350 mg/100 g b.w.) significantly increased the cumulative mean survival time and percentage of survivors and reduced the mean tumor weight, compared to tumor-bearing controls, however, a more pr...
journal_title：Biological research
pub_type： 杂志文章
doi：10.4067/s0716-97602003000200015
更新日期：2003-01-01 00:00:00
abstract：:Object recognition memory allows discrimination between novel and familiar objects. This kind of memory consists of two components: recollection, which depends on the hippocampus, and familiarity, which depends on the perirhinal cortex (Pcx). The importance of brain-derived neurotrophic factor (BDNF) for recognition m...
journal_title：Biological research
pub_type： 杂志文章
doi：/S0716-97602010000200013
更新日期：2010-01-01 00:00:00
abstract：BACKGROUND:The characterization of the dynamic process of veins walls is essential to understand venous functioning under normal and pathological conditions. However, little work has been done on dynamic venous properties. AIM:To characterize vein compliance (C), viscosity (eta), peak-strain (W St) and dissipated (W(D...
journal_title：Biological research
pub_type： 杂志文章
doi：/S0716-97602008000200012
更新日期：2008-01-01 00:00:00
abstract：:The aim of this study was to investigate the in vitro cytotoxic activity of total extract of MeOH (70%) and partition fractions of hexan, chloroform (CHCL3), ethylacetate (EtOAc) and MeOH-H2O of brown algae species (Sargassum swartzii, Cystoseira myrica, Colpomenia sinuosa) found in the Persian Gulf against in differe...
journal_title：Biological research
pub_type： 杂志文章
doi：/S0716-97602010000100005
更新日期：2010-01-01 00:00:00
abstract：:Interstitial fluid flow stress is one of the most important mechanical stimulations of bone cells under physiological conditions. Osteocytes and osteoblasts act as primary mechanosensors within bones, and in vitro are able to respond to fluid shear stress, both morphologically and functionally. However, there is littl...
journal_title：Biological research
pub_type： 杂志文章
doi：10.4067/S0716-97602012000200008
更新日期：2012-01-01 00:00:00
abstract：BACKGROUND:Asthma is a complex disease influenced by multiple genetic and environmental factors. While Madeira has the highest prevalence of asthma in Portugal (14.6%), the effect of both genetic and environmental factors in this population has never been assessed. We categorized 98 asthma patients according to the Glo...
journal_title：Biological research
pub_type： 杂志文章
doi：10.1186/0717-6287-47-40
更新日期：2014-09-04 00:00:00
abstract：BACKGROUND:Melanoma took top position among the lethal cancers and, despite there have been some great attempts made to increase the natural life of patients with metastatic disease, long-lasting and complete remissions are few. Piceatannol, owns the similar function as resveratrol, has been defined as an anti-cancer a...
journal_title：Biological research
pub_type： 杂志文章
doi：10.1186/s40659-017-0141-8
更新日期：2017-10-17 00:00:00
abstract：BACKGROUND:The Hawthorn (Crateagus sp.) mostly occurs around the temperate region of the world with a high number of species, producing a fruit with numerous beneficial effects for human health. The aim of the study was to determine organic acid and sugar contents in the fruit of a number of hawthorn species grown in E...
journal_title：Biological research
pub_type： 杂志文章
doi：10.1186/0717-6287-47-21
更新日期：2014-05-30 00:00:00
abstract：:A peptide from hindguts of the Triatoma infestans, the hematophagous Chagas' insect vector, activates adenylyl cyclase activity in Trypanosoma cruzi epimastigote membranes and stimulates the in vitro differentiation of epimastigotes (proliferative and non-infectious forms) to metacyclic trypomastigotes (non-proliferat...
journal_title：Biological research
pub_type： 杂志文章
doi：
更新日期：1993-01-01 00:00:00
abstract：:Duchenne muscular dystrophy (DMD) is secondary to loss-of-function mutations in the dystrophin gene. The causes underlying the progression of DMD, differential muscle involvement, and the discrepancies in phenotypes among species with the same genetic defect are not understood. The mdx mouse, an animal model with dyst...
journal_title：Biological research
pub_type： 杂志文章,评审
doi：10.4067/s0716-97602005000400010
更新日期：2005-01-01 00:00:00
abstract：:Hepcidin (Hepc) is a 25 amino acid cationic peptide with broad antibacterial and antifungal actions. A likely role for Hepc in iron metabolism was suggested by the observation that mice having disruption of the gene encoding the transcription factor USF2 failed to produce Hepc mRNA and developed spontaneous visceral i...
journal_title：Biological research
pub_type： 杂志文章
doi：10.4067/s0716-97602006000100022
更新日期：2006-01-01 00:00:00
abstract：:Shigella flexneri causes bacillary dysentery in humans. Essential to the establishment of the disease is the invasion of the colonic epithelial cells. Here we investigated the role of the lipopolysaccharide (LPS) O antigen in the ability of S. flexneri to adhere to and invade polarized Caco-2 cells. The S. flexneri 2a...
journal_title：Biological research
pub_type： 杂志文章
doi：10.4067/S0716-97602012000100003
更新日期：2012-01-01 00:00:00
abstract：BACKGROUND:Zinc finger RNA binding protein (ZFR) is involved in the regulation of growth and cancer development. However, little is known about ZFR function in pancreatic cancer. METHODS:Herein, to investigate whether ZFR is involved in tumor growth, Oncomine microarray data was firstly used to evaluate ZFR gene expre...
journal_title：Biological research
pub_type： 杂志文章
doi：10.1186/s40659-016-0086-3
更新日期：2016-05-13 00:00:00
abstract：BACKGROUND:This study aimed to determine the seasonal changes of total antioxidant activity and phenolic compounds in samples taken from leaves (April, July, October) and stems (April, July, October, January) of some almond (Prunus amygdalus L.) varieties (Nonpareil, Ferragnes and Texas). RESULTS:It was indicated that...
journal_title：Biological research
pub_type： 杂志文章
doi：10.1186/0717-6287-47-9
更新日期：2014-04-01 00:00:00
abstract：:Invasive properties of 15 strains of thermotolerant Campylobacter (12 C. jejuni and 3 C. coli) were studied using HeLa cells cultures. In four of them (3 C. jejuni and 1 C. coli), randomly selected, intestinal perfusion experiments were conducted in order to asses enterotoxigenicity. All strains were able to invade He...
journal_title：Biological research
pub_type： 杂志文章
doi：
更新日期：1995-01-01 00:00:00
