Abstract:
:The anti-tumor effect of R-Phycoerythrin (R-PE) from Porphyra haitanensis was studied using cell line HeLa as an in vitro model and Sarcoma-180 (S180) tumor-bearing mice as an in vivo model. The results showed that the combination treatment of R-PE and photodynamic therapy PDT) significantly inhibited the growth of HeLa cells up to 81.5%, with a fair dose-effect relationship, but did not inhibit endothelial cells. The annexin v-fitc/PI fluorescence staining experiments demonstrated that at doses between 0~60µg/mL, apoptosis cells and later stage apoptosis cells or necrosis cells increased significantly as the R-PE dosage increased. DNA electrophoresis showed that after R-PE+PDT treatment of HeLa cells for 24 hours, a light "smear" band between 100~400bp appeared to indicate the degradation of genomic DNA. The QRT-PCR results showed that R-PE+PDT treatment increased caspase-3 and caspase-10 gene expression and decreased the Bcl-2 gene expression level significantly as the R-PE dose increased, implying that R-PE promoted HeLa cell apoptosis. Compared with untreated S180 tumor-bearing mice, R-PE injection significantly inhibited the growth of S180 in tumor-bearing mice up to 41.3% at a dose of 300mg-kg⁻¹. Simultaneously, the significant increase of superoxide dismutase (SOD) activity in serum (p < 0.01) and the decrease of the malondialdehyde (MDA) level in liver suggests that R-PE improved the anti-oxidant ability of the S180 tumor-bearing mice, which may related to its antitumor effect. In addition, the R-PE caused a significant increase (p < 0.05) in the spleen index and thymus index, and a significant increase (p < 0.01) in lymphocyte proliferation, NK cell kill activity and the TNF-α level in the serum of S180 tumor-bearing mice. These results strongly suggest that the antitumor effect of R-PE from Porphyra haitanensis functioned by increasing the immunity and antioxidant ability of S180 tumor-bearing mice, promoting apoptosis by increasing protease gene expression and TNF-α secretion.
journal_name
Biol Resjournal_title
Biological researchauthors
Pan Q,Chen M,Li J,Wu Y,Zhen C,Liang Bdoi
10.4067/S0716-97602013000100013subject
Has Abstractpub_date
2013-01-01 00:00:00pages
87-95issue
1eissn
0716-9760issn
0717-6287pii
S0716-97602013000100013journal_volume
46pub_type
杂志文章,评审abstract::Hematopoietic stem cell transplantation is the accepted therapy of choice for a variety of malignant and non-malignant diseases in children and adults. Initially developed as rescue therapy for a patient with cancer after high doses of chemotherapy and radiation as well as the correction of severe deficiencies in the ...
journal_title:Biological research
pub_type: 历史文章,杂志文章
doi:10.4067/S0716-97602012000300012
更新日期:2012-01-01 00:00:00
abstract::Angiotensin-(1-7) [Ang-(1-7)] possesses novel biological functions that are distinct from angiotensin II (Ang II). In coronary arteries, the octapeptide Ang II and the heptapeptide Ang-(1-7) exert opposing actions. Ang II elicits vasoconstriction and Ang-(1-7) is a vasodilator. Ang-(1-7) elicits vasodilation by an end...
journal_title:Biological research
pub_type: 杂志文章,评审
doi:
更新日期:1998-01-01 00:00:00
abstract:BACKGROUND:Accumulating studies have demonstrated that high-mobility group A2 (HMGA2), an oncofetal protein, plays a role in tumor development and progression. However, the molecular role of HMGA2 in ovarian carcinoma is yet to be established. MicroRNAs (miRNAs), a group of small noncoding RNAs, negatively regulate gen...
journal_title:Biological research
pub_type: 杂志文章
doi:10.1186/s40659-018-0199-y
更新日期:2018-11-24 00:00:00
abstract::The neuropeptide oxytocin (OXT) is a key factor in the initiation and regulation of sociosexual behavior. The present study analyzes the effects of cohabitation and social challenge on plasma OXT concentration rates in guinea pig pairs in relation to male sociosexual behavior. The cohabitation phase lasted 3 days. On ...
journal_title:Biological research
pub_type: 杂志文章
doi:10.4067/s0716-97602006000200007
更新日期:2006-01-01 00:00:00
abstract::Bilateral aspiration lesions of the fimbria-fornix were performed in 10 male Sprague Dawley rats weighing 240-300 g under chloral hydrate narcose (420 mg/kg). Another 9 animals were operated in the same way, but no aspiration was carried out to constitute a control group. A week after surgery recording and stimulation...
journal_title:Biological research
pub_type: 杂志文章
doi:
更新日期:1996-01-01 00:00:00
abstract::Object recognition memory allows discrimination between novel and familiar objects. This kind of memory consists of two components: recollection, which depends on the hippocampus, and familiarity, which depends on the perirhinal cortex (Pcx). The importance of brain-derived neurotrophic factor (BDNF) for recognition m...
journal_title:Biological research
pub_type: 杂志文章
doi:/S0716-97602010000200013
更新日期:2010-01-01 00:00:00
abstract::X-Ray diffraction is the only method currently available for the determination of the detailed three-dimensional structure of macromolecules. In this article, the principles behind the use of X-ray crystallography in the study of protein structure are presented. The results obtained in its application to several enzym...
journal_title:Biological research
pub_type: 杂志文章,评审
doi:
更新日期:1996-01-01 00:00:00
abstract::Tumor growth is the result of combined cell proliferation overwhelming cell death and neoangiogenesis. This report shows CAM angiogenesis promoted by TA3 tumor supernatant with or without low dosis of betamethasone (Minimal antiangiogenic concentration: beta-MAAC). Methylcellulose discs instilled with 10 microliters o...
journal_title:Biological research
pub_type: 杂志文章
doi:10.4067/s0716-97602001000300010
更新日期:2001-01-01 00:00:00
abstract::The ascorbate-glutathione (ASC-GSH) cycle has an important role in defensive processes against oxidative damage generated by drought stress. In this study, the changes that take place in apoplastic and symplastic ASC-GSH cycle enzymes of the leaf and petiole were investigated under drought stress causing leaf rolling ...
journal_title:Biological research
pub_type: 杂志文章
doi:/S0716-97602009000300006
更新日期:2009-01-01 00:00:00
abstract:BACKGROUND:The root of Angelica sinensis (AS), also known as "Dang-gui," was a popular herbal medicine widely used in the treatment of gynecological diseases in China, Korea, and Japan for a long time. This study aimed to determine the effects of ethyl acetate fraction from Angelica sinensis (EAAS) on the interleukin-1...
journal_title:Biological research
pub_type: 杂志文章
doi:10.1186/0717-6287-47-41
更新日期:2014-09-05 00:00:00
abstract:BACKGROUND:The Tridax procumbens flavonoids (TPF), are well known for their medicinal properties among local natives. The TPF are traditionally used for dropsy, anaemia, arthritis, gout, asthma, ulcer, piles, and urinary problems. It also used in treating gastric problems, body pain, and rheumatic pains of joints. The ...
journal_title:Biological research
pub_type: 杂志文章
doi:10.1186/s40659-015-0043-6
更新日期:2015-09-12 00:00:00
abstract::We review the development of our knowledge and interpretations of the intermediary metabolism of Trypanosoma (Schizotrypanum) cruzi. Already in the 1950's it was clearly established that when this organism was exposed to large external concentrations of carbohydrates it was unable to catabolize them completely, even i...
journal_title:Biological research
pub_type: 杂志文章,评审
doi:
更新日期:1993-01-01 00:00:00
abstract:BACKGROUND:Prognosis remains one of most crucial determinants of gastric cancer (GC) treatment, but current methods do not predict prognosis accurately. Identification of additional biomarkers is urgently required to identify patients at risk of poor prognoses. METHODS:Tissue microarrays were used to measure expressio...
journal_title:Biological research
pub_type: 杂志文章
doi:10.1186/s40659-019-0249-0
更新日期:2019-08-09 00:00:00
abstract:BACKGROUND:Despite manifold benefits of nanoparticles (NPs), less information on the risks of NPs to human health and environment has been studied. Cobalt oxide nanoparticles (Co3O4-NPs) have been reported to cause toxicity in several organisms. In this study, we have investigated the role of Co3O4-NPs in inducing phyt...
journal_title:Biological research
pub_type: 杂志文章
doi:10.1186/s40659-016-0080-9
更新日期:2016-03-18 00:00:00
abstract:BACKGROUND:Zinc finger RNA binding protein (ZFR) is involved in the regulation of growth and cancer development. However, little is known about ZFR function in pancreatic cancer. METHODS:Herein, to investigate whether ZFR is involved in tumor growth, Oncomine microarray data was firstly used to evaluate ZFR gene expre...
journal_title:Biological research
pub_type: 杂志文章
doi:10.1186/s40659-016-0086-3
更新日期:2016-05-13 00:00:00
abstract::Exposure to arsenic via drinking water is considered as a worldwide problem. Studies have shown that arsenic exposure during pregnancy affects embryogenesis and offspring development in rats and mice. Zinc as a micronutrient regulates many physiological functions, including an antioxidative role under various toxic co...
journal_title:Biological research
pub_type: 杂志文章
doi:10.4067/S0716-97602013000200003
更新日期:2013-01-01 00:00:00
abstract::The development of plant transformation in the mid-1980s and of many new tools for cell biology, molecular genetics, and biochemistry has resulted in enormous progress in plant biology in the past decade. With the completion of the genome sequence of Arabidopsis thaliana just around the corner, we can expect even fast...
journal_title:Biological research
pub_type: 杂志文章,评审
doi:
更新日期:1999-01-01 00:00:00
abstract::Extracts prepared from seeds of Manilkara zapota, Anona squamosa, and Tamarindus indica were screened for their antibacterial activity by disc diffusion and broth dilution methods. Acetone and methanol extracts of T. indica seeds were found active against both gram-positive and gram-negative organisms. MIC values of p...
journal_title:Biological research
pub_type: 杂志文章
doi:/S0716-97602010000200003
更新日期:2010-01-01 00:00:00
abstract::This study examined the effects of estrogen supplementation on markers of neutrophil infiltration and damage in skeletal muscle of rats following ischemia. Male and female gonad-intact rats, with or without 14 days of estrogen supplementation were subjected to two hours of hind-limb ischemia and sacrificed at 24, 48 o...
journal_title:Biological research
pub_type: 杂志文章
doi:10.4067/s0716-97602005000200011
更新日期:2005-01-01 00:00:00
abstract::Skeletal muscles have the potential to regenerate by activation of quiescent satellite cells, however, the molecular signature that governs satellite cells during muscle regeneration is not well defined. Myosin light chains (Myls) are sarcomere-related proteins as traditional regulator of muscle contraction. In this r...
journal_title:Biological research
pub_type: 杂志文章
doi:/S0716-97602009000100013
更新日期:2009-01-01 00:00:00
abstract::In the last few decades, several works on event related potentials (ERPs hereafter) during sleep have been reported. In spite of numerous studies, clear methodological rules for this kind of study are often missing, making it difficult to valorize the scope of these results. We propose here a description of methodolog...
journal_title:Biological research
pub_type: 杂志文章
doi:/S0716-97602008000300004
更新日期:2008-01-01 00:00:00
abstract::Loss of Imprinting (LOI) of IGF2 and over-expressed IGF2 are associated with tumorigenesis. Our previous epidemiological study found a relatively high frequency of IGF2 LOI in healthy mid-gestation pregnant women. The aim of this study is to determine whether the expression of IGF2 is associated with its imprinting st...
journal_title:Biological research
pub_type: 杂志文章
doi:10.4067/S0716-97602012000400004
更新日期:2012-01-01 00:00:00
abstract::Although evidence is accumulating that prenatal testosterone (T) compromises reproductive function in the female, the effects of excess T in utero on the postnatal development of male reproductive function has not been studied. The aim of this study was to assess the influence of prenatal T excess on age-related chang...
journal_title:Biological research
pub_type: 杂志文章
doi:/S0716-97602007000400007
更新日期:2007-01-01 00:00:00
abstract::The objective of this study was to test the hypothesis that root of maguey (Agave salmiana Otto ex Salm-Dyck) seedlings reacts during the first 24 h to low substrate water potential (PsiW), by anatomical modifications. Three-4 cm root length seedlings were planted in vermiculite for 24 h at PsiW between -0.03 and -2.3...
journal_title:Biological research
pub_type: 杂志文章
doi:/S0716-97602009000200013
更新日期:2009-01-01 00:00:00
abstract:BACKGROUND:miR-214 was demonstrated to be upregulated in models of renal disease and promoted fibrosis in renal injury independent of TGF-β signaling in vivo. However, the detailed role of miR-214 in acute kidney injury (AKI) and its underlying mechanism are still largely unknown. METHODS:In this study, an I/R-induced...
journal_title:Biological research
pub_type: 杂志文章
doi:10.1186/s40659-018-0179-2
更新日期:2018-09-04 00:00:00
abstract:BACKGROUND:CCL2 was up-regulated in neurons and involved in microglia activation and neurological decline in mice suffering from hepatic encephalopathy (HE). However, no data exist concerning the effect of neuron-derived CCL2 on microglia activation in vitro. METHODS:The rats were pretreated with CCL2 receptor inhibit...
journal_title:Biological research
pub_type: 杂志文章
doi:10.1186/s40659-017-0130-y
更新日期:2017-09-04 00:00:00
abstract:BACKGROUND:Recently, a continuous growth of interest has been observed in antimicrobial peptides (AMPs) in the light of an alarming increase in resistance of bacteria and fungi against antibiotics. AMPs are used as biomarkers in diagnosis and monitoring of oral cavity pathologies. Therefore, the determination of specif...
journal_title:Biological research
pub_type: 杂志文章
doi:10.1186/s40659-015-0050-7
更新日期:2015-10-31 00:00:00
abstract:BACKGROUND:Osteoarthritis (OA) is a common arthritic disease and multifactorial whole-joint disease. Interactions of chemokines and OA is inadequately documented. RESULTS:In vivo and in vitro studies were conducted to investigate monocyte chemoattractant protein 1 (MCP-1) and receptor chemokine (C-C motif) receptor 2 ...
journal_title:Biological research
pub_type: 杂志文章
doi:10.1186/s40659-015-0057-0
更新日期:2015-11-17 00:00:00
abstract:BACKGROUND:The study was conducted to evaluate the in vitro thrombolytic activity, and in vivo analgesic, anti-inflammatory and antipyretic potentials of different hydrocarbon soluble extracts of Litsea glutinosa leaves for the first time widely used in the folkloric treatments in Bangladesh. This work aimed to create ...
journal_title:Biological research
pub_type: 杂志文章
doi:10.1186/0717-6287-47-56
更新日期:2014-10-29 00:00:00
abstract::Hepcidin (Hepc) is a 25 amino acid cationic peptide with broad antibacterial and antifungal actions. A likely role for Hepc in iron metabolism was suggested by the observation that mice having disruption of the gene encoding the transcription factor USF2 failed to produce Hepc mRNA and developed spontaneous visceral i...
journal_title:Biological research
pub_type: 杂志文章
doi:10.4067/s0716-97602006000100022
更新日期:2006-01-01 00:00:00