Abstract：
:All-trans retinoic acid (ATRA) or phorbol-12-myristate-13-acetate (PMA) are two representative inducers which induce the differentiation of HL-60 cells to myelocytes and monocytes respectively. Structural modifications of the pentasaccharide core portion of N-glycans on cell surface glycoproteins during differentiation of HL-60 cells induced by these two agents and the enzymatic mechanism of the structural modifications were studied. Both ATRA and PMA enhanced the incorporation of [3H]-labeled mannose into N-glycans of glycoproteins from cell surface. By using E-PHA and WGA lectin affinity chromatography to analyze [3H]-N-glycans prepared from the surface of control, ATRA- and PMA-treated cells, it was found that the content of bisecting GlcNAc (bis-GlcNAc) attached to the core of N-glycans decreased in ATRA-treated cells, but increased in PMA-treated cells. Since GlcNAc T-III is the enzyme responsible for the synthesis of bis-GlcNAc, the change of bis-GlcNAc was consistent with the alteration of GlcNAc T-III activities in ATRA- and PMA-treated cells. Core alpha-1,6 fucose of surface N-glycans decreased during differentiation which was determined by AAL affinity chromatography. This was also compatible with the reduced activity of alpha-1,6 Fuc T. the enzyme catalyzing the synthesis of core Fuc, in ATRA- and PMA-treated cells. The increase of GlcNAc T-III activity and bis-GlcNAc content in N-glycans may be related to cell adhesion observed in monocytic differentiation.
journal_name
Leuk Resjournal_title
Leukemia researchauthors
Li Z,Liu AH,Liu F,Chen HLdoi
10.1016/s0145-2126(98)00068-xsubject
Has Abstractpub_date
1998-08-01 00:00:00pages
727-34issue
8eissn
0145-2126issn
1873-5835pii
S0145-2126(98)00068-Xjournal_volume
22pub_type
杂志文章abstract：:In previous studies, HL60 AML cells treated with tumor necrosis factor-alpha (TNF), interferon-gamma (IFN), and lipopolysaccharides (LPS) displayed decreased growth and viability, enhanced monocytic pathway differentiation and endogenous TNF release. Endogenous TNF release by LPS/TNF/IFN treated HL60 cells was postula...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/0145-2126(94)90004-3
更新日期：1994-01-01 00:00:00
abstract：:The levels of expression of the activation antigen CD98 were studied by mAB CAF7 in 51 newly diagnosed consecutive cases of childhood acute lymphoblastic leukemia aged from 1 to 13 years. The mean follow-up was 8 months. A wide range of CAF7 expression was observed, the highest mean fluorescence intensity exceeding th...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/0145-2126(95)00114-x
更新日期：1996-01-01 00:00:00
abstract：:Ectopic expression of LMO2 occurs in approximately 45% of T-lineage acute lymphoblastic leukemias (T-ALL), sometimes in association with chromosomal translocations. Recently, a lymphoproliferative disorder developed in two participants in a gene therapy trial due to LMO2 activation via integration of the retroviral ve...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2004.05.013
更新日期：2005-01-01 00:00:00
abstract：:We retrospectively analyzed allogenic stem cell transplantation (allo-SCT) outcomes in 82 patients with AML or MDS were conditioned with fludarabine, idarubicin, intravenous-busulfan and cytarabine (FIBA) or busulfan and cyclophosphamide (BuCy). Compared to BuCy regimen, reduced intensity conditioning (RIC) with FIBA ...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2013.08.016
更新日期：2013-11-01 00:00:00
abstract：:The aim of this study is to detect the biologic and/or prognostic significance of survivin (S) and effector protease receptor 1: EPR-1 (E) expression in acute leukemias (34 ALL and 40 AML) by using RT-PCR. S and E expressions were found in 83.8 and 20.3% of the cases, respectively. S was detected in 90%, 76.5% and E w...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2007.01.005
更新日期：2007-11-01 00:00:00
abstract：:Hematopoiesis is regulated by several cytokines with pleiotropic activity. Several evidences have clearly demonstrated that these molecules, formerly regarded as specific for the hematopoietic system, also affect certain endothelial cell functions and that hematopoietic factors clearly influence angiogenesis. This rev...
journal_title：Leukemia research
pub_type： 杂志文章,评审
doi：10.1016/j.leukres.2012.02.003
更新日期：2012-05-01 00:00:00
abstract：:Using the human erythroleukaemic cell line K562 cl.6 and its daunorubicin-resistant subline K/DAU600, and the human T-lymphoblastic leukaemic cell line CCRF-CEM and its vinblastine-resistant subline CEM/VLB100, we have shown that the drug-resistant cell lines were more sensitive to cytotoxicity induced by tumour necro...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/0145-2126(94)00149-5
更新日期：1995-03-01 00:00:00
abstract：:Bcr-Abl tyrosine kinase inhibitor induces apoptosis and erythroid differentiation of K562 cells. During this erythroid differentiation, c-Myc and cyclin D1 transcripts are transiently downregulated. Accordingly, we studied the effect of cyclin D1 overexpression on erythroid differentiation. After treatment with 250 nM...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2003.10.022
更新日期：2004-06-01 00:00:00
abstract：BACKGROUND:Current results regarding treatment outcomes in acute myeloid leukemia (AML) point to significant differences between low- and middle-income countries (LMIC) and high-income countries (HIC). Excluding well-known socioeconomic issues, genetic markers important for prognosis have not been properly incorporated...
journal_title：Leukemia research
pub_type： 杂志文章,多中心研究
doi：10.1016/j.leukres.2017.07.005
更新日期：2017-09-01 00:00:00
abstract：:A novel bone biopsy technique was used to generate a robust stromal cell system to study how stroma modulates CLL B-cell apoptosis and how the leukemic cell-stromal interaction influences secretion of vascular factors. Marrow stromal elements (MSE) rescued CLL B-cells from both spontaneous and drug induced apoptosis, ...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2006.11.024
更新日期：2007-07-01 00:00:00
abstract：:Semenogelin (SEMG) I is a Cancer-Testis (CT) antigen that we have found to be expressed in myeloma cells. In this study, we set out to determine whether the expression of SEMG I could be upregulated pharmacologically. We found that SEMG I expression in myeloma cells can be upregulated by 5-azacytidine, IL-4 and IL-6. ...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2008.03.036
更新日期：2008-12-01 00:00:00
abstract：:The tumor microenvironment (TME) is critical to the longevity of tumor B cells in chronic lymphocytic leukemia (CLL). Bone marrow mesenchymal stem cells (BMMSCs) and the cytokines they produce including IL-6 are important components of the TME in CLL. We found BMMSCs supported the survival of CLL cells in vitro throug...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2018.01.006
更新日期：2018-03-01 00:00:00
abstract：:There is resurgent interest in glucocorticoids (GCs) in the treatment of poor prognosis chronic lymphocytic leukemia (CLL). Little is known however on how GCs induce apoptosis in CLL. Methylprednisolone (MP) induces apoptosis in ZAP-70 positive CLL more readily than in ZAP-70 negative CLL, which is in contrast to the ...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2009.02.022
更新日期：2009-10-01 00:00:00
abstract：:Promoter hypermethylation occurs early in leukemogenesis and seems to be associated with poor prognosis in acute lymphoblastic leukemia (ALL). The methylation status of the promoter region of six genes was analyzed in 71 children with ALL using methylation specific PCR (MSP). Calcitonin (CALCA) and E-cadherin (CDH1) w...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2005.11.016
更新日期：2006-07-01 00:00:00
abstract：:The role of hemin induction of K562 in inositol phospholipid metabolism has not been previously studied. K562 cells were induced to synthesize hemoglobin upon addition of bovine hemin to the culture media. The phospholipid content of K562 was determined before and after the addition of hemin. The results of this study...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/0145-2126(91)90055-x
更新日期：1991-01-01 00:00:00
abstract：:Aggressive chemotherapy of myelodysplastic syndromes is rarely feasible because these disorders predominantly occur in elderly patients who often have concurrent illnesses. Alternative treatment modalities must therefore be evaluated. This review summarizes the results that have been obtained with low-dose chemotherap...
journal_title：Leukemia research
pub_type： 杂志文章,评审
doi：10.1016/0145-2126(92)90058-f
更新日期：1992-01-01 00:00:00
abstract：:The MYBL2 gene encodes a transcription factor implicated in cell proliferation and maturation whose amplification or overexpression has been associated with different human malignancies, suggesting that it could be implicated in tumorigenesis. We analyzed MYBL2 expression and its prognostic value in 291 patients with ...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2013.09.015
更新日期：2013-12-01 00:00:00
abstract：:We analyzed surface antigens, multidrug resistance (MDR) parameters (PGP, MRP, LRP), tissue infiltration parameters (CD18, CD44, VCAM, MMP2), receptors for colony stimulating factors (G-CSFr, GM-CSFr) and cell cycle parameters (Ki-67, topoisomerase IIalpha) in 86 patients with acute lymphoblastic leukemia (ALL). LRP, ...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/s0145-2126(03)00026-2
更新日期：2003-10-01 00:00:00
abstract：:CD13/aminopeptidase-N, an enzyme expressed by myeloid cells, may be important in the regulation and signalling pathways that control myeloid growth and differentiation. In this study we have used the myeloid leukaemic cell line HL60, and its ability to differentiate when induced by all-trans-retinoic acid (ATRA), to s...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/0145-2126(94)90045-0
更新日期：1994-08-01 00:00:00
abstract：:The acid phosphatase (AP), beta-glucuronidase (BG), and alpha-naphthyl-acetate esterase (ANAE) distribution and the morphology of stage 0 B-CLL lymphocytes were studied. The results were compared with the same hydrolase equipment and morphology of normal B-cell populations showing the B-CLL-like phenotype, and thus re...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/0145-2126(87)90074-9
更新日期：1987-01-01 00:00:00
abstract：:A number of inborn errors of purine metabolism have been associated with immunodeficiency diseases. From studies to the possible mechanism(s) leading to the defects in the immune system, it appeared that the accumulation of deoxyATP and deoxyGTP and the subsequent inhibition of ribonucleotide reductase played an impor...
journal_title：Leukemia research
pub_type： 杂志文章,评审
doi：10.1016/0145-2126(83)90042-5
更新日期：1983-01-01 00:00:00
abstract：:Methods for ex vivo purging of neoplastic cells from harvested marrow are being developed to increase the efficacy of autologous transplantation. One approach is selective photosensitization, using sensitizing compounds and light radiation. Pyrene-containing fatty acids and lipids are potent photosensitizers, e.g. 12-...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/0145-2126(92)90170-c
更新日期：1992-01-01 00:00:00
abstract：:MicroRNAs (miRNAs) are small endogenous RNA molecules that regulate gene expression at the post-transcriptional level through its sequence complementation with target mRNAs. An individual miRNA species can simultaneously influence the expression of multiple genes and conversely, several miRNAs can synchronously contro...
journal_title：Leukemia research
pub_type： 杂志文章,评审
doi：10.1016/j.leukres.2013.08.020
更新日期：2013-11-01 00:00:00
abstract：:AG-013736 is an oral anti-angiogenesis agent with activity against a variety of receptor tyrosine kinases, including VEGFR-1, VEGFR-2, VEGFR-3, c-kit, and PDGFR-beta. A phase 2 study was conducted in patients with poor prognosis AML or MDS. Twelve patients (six AML; six MDS) were treated with AG-013736 at a dose of 10...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2005.10.024
更新日期：2006-07-01 00:00:00
abstract：:Myelodysplastic syndrome (MDS) in children has been reported to be associated with various constitutional anomalies; however, it has not been described previously in patients with Griscelli syndrome (GS). In this report, we present a case with GS and refractory anemia with an excess of blasts. We believe that careful ...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/s0145-2126(98)00075-7
更新日期：1998-09-01 00:00:00
abstract：:Little information is available about the risk factors and means to improve the survival rate of acute leukemia in a rare but often fatal complication after liver transplantation (LT). We report the development of AML-M2 in one of the 764 patients who underwent liver transplantation at our center, and review the liter...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2009.03.035
更新日期：2009-10-01 00:00:00
abstract：:Chemosensitivity of purified AML blasts to daunomycin was examined under conditions of CSF stimulation and compared with clinical outcome of DNR combination chemotherapy. AML blasts from 16 patients were purified, incubated serum-free in the presence of optimal concentrations of a complete cocktail of IL-3, GM-CSF and...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/0145-2126(90)90166-7
更新日期：1990-01-01 00:00:00
abstract：:Fifty-three patients with Ph positive chronic myeloid leukaemia in blastic phase were studied. Additional abnormalities were found in 29 (55%) patients and were more common in myeloid (64%) than lymphoid (45%) blast crisis. The most frequent were +Ph (32%), +8 (28%), +19 (19%), +20 (9%) and +21 (9%). i(17q) (9%) was a...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/0145-2126(94)90023-x
更新日期：1994-05-01 00:00:00
abstract：:The amplified myeloma centrosome has been identified as a therapeutic target. The present study explored the expression and prognostic significance of the centrosome-associated protein PLK1 in myeloma and the effect of BI 2536, a potent and selective inhibitor of PLK1, on myeloma cells. High plasma cell expression of ...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2011.07.016
更新日期：2011-12-01 00:00:00
abstract：:Feulgen-DNA cytophotometry and 3H-thymidine autoradiography were used to evaluate therapeutic cytapheresis effects on cell cycle distributions of peripheral blood leukemic cells in 15 acute leukemia, 5 CML and 8 CLL patients and of bone marrow erythroblasts in 18 PV patients. Both individual cytapheresis procedures an...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/0145-2126(87)90186-x
更新日期：1987-01-01 00:00:00
