Abstract:
:Feulgen-DNA cytophotometry and 3H-thymidine autoradiography were used to evaluate therapeutic cytapheresis effects on cell cycle distributions of peripheral blood leukemic cells in 15 acute leukemia, 5 CML and 8 CLL patients and of bone marrow erythroblasts in 18 PV patients. Both individual cytapheresis procedures and a long-term program of cytapheresis altered cell cycle distributions and biochemistry which may be summarized as follows: An increase in percentage of cells in S-phase (acute leukemia, CML); a decrease in the differences between values of S-cell number obtained by cytophotometry and autoradiography (acute leukemia, CML, PV); a decrease in some cases in enlarged fraction of cells in G2 (blast crisis of CML, PV); an activation of G0----G1 transition and an increase in the PAS-positive lymphocyte fraction in CLL patients. An apparent increase of blasts in S-phase was found after a pheresis in acute leukemia patients who responded to subsequent chemotherapy. In "nonresponders", a pheresis had no effect on cell cycle distributions. The results of the study demonstrate that despite the wide variability of individual responses, cytapheresis activates cell proliferation and metabolic processes in patients with hematological malignancies.
journal_name
Leuk Resjournal_title
Leukemia researchauthors
Kotelnikov VM,Kasatkina VV,Dultsina SM,Sarytcheva TG,Kalinin NN,Kovaleva LG,Poljanskaja AM,Isajev VG,Khoroshko ND,Lishmanova NGdoi
10.1016/0145-2126(87)90186-xsubject
Has Abstract,Author List Incompletepub_date
1987-01-01 00:00:00pages
397-403issue
4eissn
0145-2126issn
1873-5835journal_volume
11pub_type
杂志文章abstract::Gain-of-function mutations in the proto-oncogene c-kit that induce constitutive kinase activity of its product, KIT protein, are characteristic of human mast cell disease and are believed to play a central role in mast cell leukemia oncogenesis, proliferation and survival. Nuclear overexpression of the Wnt effector be...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2007.08.023
更新日期:2008-05-01 00:00:00
abstract::CFW/D mice injected neonatally with a type B retrovirus, (DMBA-LV), rapidly develop thymic lymphomas. The present study examined simultaneously the karyotype and the expression of surface antigens on thymocytes from DMBA-LV treated mice at 28, 35 and 42 days of age during the development of lymphomas, and of cells fro...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/0145-2126(89)90122-7
更新日期:1989-01-01 00:00:00
abstract::Aneuploidy is a very common prognostic factor in multiple myeloma (MM). Nonhyperdiploidy including near-tetraploidy (NT) is a poor prognostic indicator, compared to hyperdiploidy in multiple myeloma (MM). NT results from endoduplication of hypodiploidy. We report of a 55-year-old female patient diagnosed with advanced...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2010.01.013
更新日期:2010-07-01 00:00:00
abstract::We investigated the possible influence of recombinant (r) sIL-6R on the growth of three IL-6 non-responsive or weakly IL-6 responsive long-term myeloma cell lines. The three cell lines chosen for the study (U266, L363 and Fravel) all expressed gp130 but differed in their expression of IL-6R and IL-6. mRNA analysis by ...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/0145-2126(95)00077-1
更新日期:1996-04-01 00:00:00
abstract::Kaposi's sarcoma-associated herpesvirus (KSHV) is associated with three distinct lymphoproliferative disorders: primary effusion lymphoma (PEL), multicentric Castleman's disease (MCD) and germinotropic lymphoproliferative disorder (GLD). KSHV positive lymphocytes in GLD and in most cases of PEL are co-infected by Epst...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2003.08.003
更新日期:2004-04-01 00:00:00
abstract::Leukemic blasts of two patients with acute leukemia exhibited similar characteristics. They were heterogeneous in size with a diameter of 14-30 microns in smears and unclassifiable by morphological, cytochemical, immunophenotypic and ultrastructural examinations. Cytogenetic examinations of both revealed a near-tetrap...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/0145-2126(94)90087-6
更新日期:1994-07-01 00:00:00
abstract::A clonal origin of hematopoiesis was studied by investigation of X-chromosome inactivation patterns (XCIP) in isolated granulocyte, CD14(+) and CD3(+) subpopulations obtained from bone marrow and peripheral blood of 36 female patients with primary myelodysplastic syndrome (MDS). Clonality was assessed by PCR amplifica...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2004.08.008
更新日期:2005-04-01 00:00:00
abstract::Useful prognostic markers for patients with diffuse large B cell lymphoma (DLBCL) have been reported. To identify which biomarker best predicts the prognosis of patients with DLBCL, we performed a retrospective study that included 319 DLBCL patients who had received rituximab, cyclophosphamide, doxorubicin, vincristin...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2015.08.016
更新日期:2015-08-29 00:00:00
abstract::We have analysed the configuration of immunoassociated genes and the karyotypes of 30 patients with acute myelocytic leukemia (AML) and 10 with chronic myelocytic leukemia in blast crisis (CML-BC). In AML, the frequencies of T-cell receptor (TcR) beta, gamma, and delta chain and immunoglobulin heavy and light chain ge...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/0145-2126(91)90070-a
更新日期:1991-01-01 00:00:00
abstract::Malignant B-1 cells derived from NZB mice, a murine model of chronic lymphocytic leukemia, produce significantly higher levels of IL-10 mRNA than normal B-1 or B cells. IL-10 may act as an autocrine growth factor for malignant B-1 cells. By addition of antisense oligodeoxynucleotides specific for IL-10 mRNA, we were a...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/0145-2126(94)00129-x
更新日期:1995-03-01 00:00:00
abstract::We recently confirmed that ciclopirox olamine inhibits Wnt/beta catenin signalling in myeloma. Griseofulvin (GF) has similar chemical features as compared to ciclopirox olamine. In this study the anti-tumor effect of GF was investigated. GF demonstrated a major apoptotic activity in various human and murine myeloma an...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2010.10.008
更新日期:2011-08-01 00:00:00
abstract::We wondered whether there was any association between the extent of increased angiogenesis and IgV(H) gene mutational status, expression of CD38 and ZAP-70 in early B-cell chronic lymphocytic leukemia (CLL) patients. Circulating levels of vascular endothelial growth factor (VEGF) correlated positively with ZAP-70-expr...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2007.03.009
更新日期:2007-11-01 00:00:00
abstract::Exogenous expression of the transcription factor Scl (Tal1) in WEHI-3B D+ myelomonocytic leukemia cells interferes with their capacity to respond to all-trans retinoic acid (ATRA) induced differentiation; combination of ATRA with LiCl, however, circumvents the inhibition of differentiation produced by Scl. To gain inf...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2004.03.017
更新日期:2004-11-01 00:00:00
abstract::Using a short term liquid system we have shown that blood from some patients with primary myelofibrosis (PMF) and chronic granulocytic leukaemia (CGL) in megakaryoblastic transformation (CGL-Mk) gives rise to large numbers of progenitor cells committed to the megakaryocyte (Mk) lineage. As assessed by indirect immunof...
journal_title:Leukemia research
pub_type: 历史文章,杂志文章
doi:10.1016/0145-2126(85)90108-0
更新日期:1985-01-01 00:00:00
abstract::The aim of this study was to evaluate effect of c-KIT mutations on RUNX1/RUNX1T1 fusion transcript expression in patients with t(8;21)-positive AML. Fifty patients diagnosed with t(8;21)-positive AML for recent 10 years were enrolled. Patients with c-KIT mutations tended to achieve a greater than 3-log reduction in RU...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2013.02.010
更新日期:2013-07-01 00:00:00
abstract::This study was conducted to determine the frequency of the most common fusion genes in Mexican pediatric patients with acute lymphoblastic leukemia (ALL). Molecular analysis using RT-PCR was carried out in 53-blood samples: 52 patients with de novo ALL and one with relapsed ALL. The ETV6-RUNX1 fusion was found in 7 ca...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2008.03.021
更新日期:2008-10-01 00:00:00
abstract:OBJECTIVES:Follicular lymphoma (FL) is generally considered an indolent disorder but a significant subset of patients shows a worse outcome. Aim of this study was to validate the FLIPI score in an independent series of follicular lymphoma patients and to correlate prognostic categories with the period of diagnosis and ...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2005.07.006
更新日期:2006-03-01 00:00:00
abstract::Hyperdiploid and non-hyperdiploid multiple myeloma represents distinct biological entities characterized by different patterns of genetic changes. We sought to determine whether ploidy category (non-hyperdiploid versus hyperdiploid) remains stable over time from diagnosis to progression. Of the 43 patients studied (39...
journal_title:Leukemia research
pub_type: 临床试验,杂志文章
doi:10.1016/j.leukres.2005.07.004
更新日期:2006-03-01 00:00:00
abstract::The frequency of chromosomal alterations was compared among four children groups: those with Down syndrome and acute leukemia (DS/AL), those with acute leukemia (AL), those with only Down syndrome (DS) and healthy children (NC). The frequency of acquired chromosome abnormalities was larger in the AL group, followed by...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2005.03.015
更新日期:2005-11-01 00:00:00
abstract::We report the establishment and characterization, including HLA-typing, immunophenotypic and molecular cytogenetic analysis, of a novel EBV-negative cell line (BLUE-1) derived from adult relapsed sporadic Burkitt lymphoma. BLUE-1 carries the pathognomonic t(8;14)(q24;q32) effecting MYC/IgHJ fusion and a novel t(6;20)(...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2006.03.026
更新日期:2006-11-01 00:00:00
abstract::We analysed the optimal conditions for autologous bone marrow purging using complement binding monoclonal antibodies (mAbs). Twelve mAbs belonging to four clusters (CD9, CD10, CD19, CD24), alone or combined were evaluated by using direct cytotoxicity and clonogenic assays. We observed the following data: (1) optimal c...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/0145-2126(87)90117-2
更新日期:1987-01-01 00:00:00
abstract::Post-transplant leukemic relapse remains the major problem following autologous bone marrow transplantation (ABMT). One possible approach to reducing the relapse rate is to intensify pre-transplant conditioning. Thiotepa (TTP) is an alkylating agent that has been used mainly in breast and ovarian cancer with 20-50% re...
journal_title:Leukemia research
pub_type: 临床试验,杂志文章
doi:10.1016/s0145-2126(98)00102-7
更新日期:1998-11-01 00:00:00
abstract::When exposed to the anthracycline doxorubicin, K562 cells undergo differentiation which is characterized by arrested cell division, an increased mean cell diameter, and the production of hemoglobin. The influence of expression of p28, a low-molecular weight stress protein, on the differentiation of K562 cells was exam...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/s0145-2126(00)00095-3
更新日期:2001-02-01 00:00:00
abstract::Human promyelocytic leukemia cells (HL-60) mature into functional granulocytes in response to in vitro treatment with several classes of chemical agents. Compounds that increase intracellular adenosine 3':5' -cyclic monophosphate induce a modified program of maturation in which the cells demonstrate functional propert...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/0145-2126(85)90311-x
更新日期:1985-01-01 00:00:00
abstract::A single somatic mutation, V617F, in the pseudokinase domain of the Jak2 is the primary cause of many chronic myeloproliferative diseases. As valine 617 of Jak2 is conserved as valine 678 of Tyk2, we examined the effect of a homologous mutation in Tyk2 (V678F Tyk2) on cell growth. V678F Tyk2 augmented the transcriptio...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2006.08.018
更新日期:2007-08-01 00:00:00
abstract::TCL-1 expression is variable in CLL, and no study has examined its association with treatment response. We measured TCL-1 protein in CLL cells from 51 patients who then received pentostatin, cyclophosphamide, and rituximab. TCL-1 expression did not correlate with any pre-treatment characteristics. Lower TCL-1 levels w...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2007.05.020
更新日期:2007-12-01 00:00:00
abstract::Febrile neutropenia (FN) risk-assessment and granulocyte-colony stimulating factor (G-CSF) prophylaxis use in clinical practice was evaluated in patients with diffuse large B-cell lymphoma receiving R-CHOP-21. More G-CSF primary prophylaxis was used in patients assessed as high FN risk, but R-CHOP-21 was associated wi...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2012.02.002
更新日期:2012-05-01 00:00:00
abstract::We reported the different clinical features between Japanese and German refractory anemia (RA) patients in FAB classification. We re-analyzed the clinical features by WHO classification revised in 2008. The frequencies of refractory cytopenia with unilineage dysplasia (RCUD) and myelodysplastic syndrome-unclassified (...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2009.11.015
更新日期:2010-08-01 00:00:00
abstract::Checkpoint genes code for a family of proteins which sense DNA damage in eukaryotic cells. They play an important role in the control of the cell cycle. The human CHK2 is a homolog of the yeast G(2) checkpoint kinases known as CDS1 and RAD53. The CHK2 may be a tumor suppressor gene because it was found to be mutated i...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/s0145-2126(00)00130-2
更新日期:2001-04-01 00:00:00
abstract::A novel Philadelphia (Ph1) chromosome positive cell line, designated KYO-1, was established from the peripheral blood of a patient with chronic myelogenous leukemia (CML) in blastic crisis. Although this line had a unique capacity to differentiate spontaneously along the erythroid and monocytoid lineages as evidenced ...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/0145-2126(85)90314-5
更新日期:1985-01-01 00:00:00