Abstract：
:Methods for ex vivo purging of neoplastic cells from harvested marrow are being developed to increase the efficacy of autologous transplantation. One approach is selective photosensitization, using sensitizing compounds and light radiation. Pyrene-containing fatty acids and lipids are potent photosensitizers, e.g. 12-(1-pyrene)dodecanoic acid (P12), is taken up preferentially by leukemic cells and undergo photoexcitation when exposed to long wave ultra-violet light, resulting in selective killing of leukemic cells. These compounds are incorporated into the neutral- and phospho-lipids of the cells. The presence of intracellular pyrene-linked lipids might present a potential hazard in applying these agents for clinical use. We have, therefore, studied a series of other pyrene-linked compounds with the objective of finding a non-metabolizable photosensitizing agent that can be easily removed from the cells. In the present paper we report the results with pyrene butanol (P4-OH), a pyrene linked short-chain alcohol. When compared to P12, P4-OH was found to be taken up by cells most rapidly and reached saturation within minutes. It did not undergo any metabolism and washing the cells with serum-containing salt solutions removed practically all the P4-OH. This compound was found to be an efficient photosensitizer (in terms of concentrations and time of incubation with the cells) and selective to leukemic cells--it caused a 99% reduction in leukemic clonogenic cells under conditions that normal hemopoietic progenitors remained almost intact. These properties make P4-OH a potential photosensitizer for clinical application.
journal_name
Leuk Resjournal_title
Leukemia researchauthors
Fibach E,Rabia SA,Gatt Sdoi
10.1016/0145-2126(92)90170-ckeywords:
subject
Has Abstractpub_date
1992-01-01 00:00:00pages
453-62issue
5eissn
0145-2126issn
1873-5835journal_volume
16pub_type
杂志文章abstract：:We have established a new T-cell line (CL-8313) that produces interleukin-3 from the spleen cells of mice with a radiation-induced myeloproliferative disorder. IL-3 activity was detected at an extremely high level in the culture medium of the CL-8313 cell line in the absence of any exogenous stimulator. A large amount...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/0145-2126(88)90005-7
更新日期：1988-01-01 00:00:00
abstract：:Essential thrombocythemia (ET) is an uncommon chronic myeloproliferative disorder with no cure. Patients with ET are at risk of different complications, and currently there are no optimal prognostic standards to predict severe post-diagnosis complications such as thrombosis and hemorrhage. In this study, we retrospect...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2018.03.013
更新日期：2018-06-01 00:00:00
abstract：:A permanent cell line, MN 60, was established from the peripheral blood of a patient with an acute lymphoblastic leukemia (ALL) classified morphologically as being of the L3 type. Cell growth started rapidly in vitro and no feeder cells were needed. Cells of the MN-60 line were identical to the original leukemic cells...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/0145-2126(82)90085-6
更新日期：1982-01-01 00:00:00
abstract：:The purpose of this study was to determine the sensitivity to merocyanine 540 (MC 540)-mediated photolysis of normal human hematopoietic progenitor cells and four leukemia cell lines (Daudi, Raji, K562 and HL-60). Late erythroid progenitors were the most sensitive normal cells. Early erythroid progenitors were of inte...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/0145-2126(87)90104-4
更新日期：1987-01-01 00:00:00
abstract：:Acute promyelocytic leukemia (APL) is highly malignant and frequently expresses the PML-RARalpha (promyelocytic leukemia-retinoic acid receptor-alpha) fusion protein. This fusion protein is targeted by all-trans retinoic acid (ATRA) and arsenic trioxide (As2O3), presently used in APL therapy. We have evaluated effects...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2007.06.021
更新日期：2008-02-01 00:00:00
abstract：:Imatinib mesylate, a Bcr/Abl tyrosine kinase inhibitor, is widely used in treating chronic myeloid leukemia. However, drug-resistance of leukemia cells becomes an emergent problem. Herein, various flavonoids were screened for applicability in leukemia treatment, and 3-hydroxyflavone (3-HF) was found to be most effecti...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2012.05.018
更新日期：2012-09-01 00:00:00
abstract：:We designed a pharmacokinetic and pharmacodynamic phase I study of sequential topotecan (2.55-6.3mg/m2) by 72h infusion followed by five daily doses of etoposide for patients with refractory acute leukemia based upon synergistic anti-tumor activity of topoisomerase I and II inhibitors in vitro. Eight of the 29 patient...
journal_title：Leukemia research
pub_type： 临床试验,杂志文章
doi：10.1016/s0145-2126(02)00079-6
更新日期：2003-01-01 00:00:00
abstract：:CD200, a membrane glycoprotein of the immunoglobulin superfamily, is overexpressed in CLL. Soluble in serum CD200 (sCD200) is correlated with poor prognosis in CLL. ADAM (a disintegrin and metalloproteinase) enzymes are implicated in membrane protein shedding. ADAM28 mRNA expression in CLL was correlated with plasma s...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2013.04.014
更新日期：2013-07-01 00:00:00
abstract：:A single patient who had a leukemic relapse six months after receiving a syngeneic bone marrow transplant was given "adoptive chemoimmunotherapy." Lymphocytes from the patients identical twin were alloactivated and grown in T cell growth factor in vitro. After receiving chemotherapy to reduce the number of relapsed le...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/0145-2126(84)90110-3
更新日期：1984-01-01 00:00:00
abstract：:The involvement of histone lysine methyltransferases (HMT) in carcinogenesis is not well understood. Here, we describe a dose-dependent growth and survival inhibitory effects of BIX-01294, a specific inhibitor of euchromatic HMT2, in promyelocytic leukemia HL-60 and NB4 cells. BIX-01294 combined with all-trans retinoi...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2014.04.003
更新日期：2014-07-01 00:00:00
abstract：:The in-vitro effects of deoxycoformycin (dCF) on dATP, NAD, ATP and DNA strand breaks have been evaluated in the cells from 42 patients with various types of chronic lymphoid leukemia. These included 18 with B-cell chronic lymphoid leukemias of different types (BCL); 10 with hairy cell leukemia (HCL) and 14 with T-cel...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/0145-2126(87)90140-8
更新日期：1987-01-01 00:00:00
abstract：:In 141 adult patients with diagnosis of acute myeloid leukemia the overall expression and intensity of expression of CD34 antigen on leukemic cells was investigated. Myeloid blasts were tested by applying direct immunofluorescence staining using anti-CD34 fluorescein monoclonal antibody in flow cytometry. CD34 antigen...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/s0145-2126(97)00015-5
更新日期：1997-07-01 00:00:00
abstract：:The thymic stroma has long been implicated in AKR thymic leukaemia. In this study an extensive panel of monoclonal antibodies was used to investigate changes in the AKR thymic microenvironment, in parallel with thymocyte differentiation of normal (2 month), preleukaemic (5-7 month) and leukaemic (> 7 month) mice. We f...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/0145-2126(95)00102-6
更新日期：1996-10-01 00:00:00
abstract：:The technology of array comparative genomic hybridization (array-CGH/aCGH) enabled the identification of novel genomic aberrations in chronic lymphocytic leukemia (CLL) including the monoallelic and biallelic deletions affecting 22q11 locus. In contrast to previous publications, we hypothesized that the described 22q1...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2013.03.018
更新日期：2013-07-01 00:00:00
abstract：BACKGROUND:Therapy strategies for myelodysplastic syndromes (MDS) and juvenile myelomonocytic leukemia (JMML) vary considerably. OBJECTIVE:To review the treatment of Brazilian children who were diagnosed with MDS or JMML in the past decade and reported to the Brazilian Cooperative Group on Pediatric Myelodysplastic Sy...
journal_title：Leukemia research
pub_type： 杂志文章,多中心研究
doi：10.1016/j.leukres.2004.01.008
更新日期：2004-09-01 00:00:00
abstract：:During monocyte-macrophage differentiation of HL-60 cells by 12-O-tetradecanoyl phorbol 13-acetate, the intracellular globular(G)-actin and polymerized(F)-actin increased 3-fold and 1.7-fold, respectively. Time course studies showed that these changes of actin levels were nearly coincident with the development of macr...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/0145-2126(89)90121-5
更新日期：1989-01-01 00:00:00
abstract：:Nitric oxide (NO) has been shown to be cytotoxic for normal and transformed cell lines. One of the intracellular targets for NO action is glutathione (GSH). GSH determinates cellular redox potential and modulates several biological events. During oxidative and nitrosative stress, glutathione system imbalance is associ...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2005.06.021
更新日期：2006-02-01 00:00:00
abstract：:Outcomes in patients with secondary acute myeloid leukemia (sAML) (including therapy related myeloid neoplasms and AML with myelodysplasia related changes (MRC)) are poor. Patients treated with hypomethylating agents (HMAs) for antecedent hematological malignancy (AHM) have suboptimal responses to induction chemothera...
journal_title：Leukemia research
pub_type： 杂志文章,多中心研究
doi：10.1016/j.leukres.2020.106367
更新日期：2020-06-01 00:00:00
abstract：:This study was aimed to illustrate the significance of minimal residual disease (MRD) assessment on day 22 in childhood acute lymphoblastic leukemia. MRD were measured on day 22, day 36, week 12, month 6 and month 12 by four-color flow cytometry. The 5-year cumulative incidence of relapse was significantly different f...
journal_title：Leukemia research
pub_type： 临床试验,杂志文章
doi：10.1016/j.leukres.2012.03.014
更新日期：2012-08-01 00:00:00
abstract：:A novel bone biopsy technique was used to generate a robust stromal cell system to study how stroma modulates CLL B-cell apoptosis and how the leukemic cell-stromal interaction influences secretion of vascular factors. Marrow stromal elements (MSE) rescued CLL B-cells from both spontaneous and drug induced apoptosis, ...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2006.11.024
更新日期：2007-07-01 00:00:00
abstract：:Gain-of-function mutations in the proto-oncogene c-kit that induce constitutive kinase activity of its product, KIT protein, are characteristic of human mast cell disease and are believed to play a central role in mast cell leukemia oncogenesis, proliferation and survival. Nuclear overexpression of the Wnt effector be...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2007.08.023
更新日期：2008-05-01 00:00:00
abstract：:In adult T-cell leukemia/lymphoma (ATL), it is difficult to achieve remission and the reason for the resistance to chemotherapeutic agents may be linked to the presence of multidrug resistance (MDR) proteins. Lung resistance-related protein (LRP), multidrug resistance-associated protein and P-glycoprotein are three MD...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2006.10.012
更新日期：2007-04-01 00:00:00
abstract：BACKGROUND:Growing evidence demonstrates that an increased number of CD68 positive tumor-associated macrophages (TAM) is associated with decreased survival in patients with newly diagnosed classic Hodgkin lymphoma (HL). However, the impact of TAM in relapsed and refractory disease is unknown. DESIGN AND METHODS:To inv...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2013.03.021
更新日期：2013-09-01 00:00:00
abstract：:Argininosuccinate synthetase (ASS) is a ATP-dependent and rate-limiting enzyme of the urea cycle which catalyzes L-citrulline to L-arginine in combination with argininosuccinate lyase (ASL). We demonstrate here that (a) human normal T and B lymphocytes did not express ASS activity, (b) however, three adult T leukemia ...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/0145-2126(90)90184-b
更新日期：1990-01-01 00:00:00
abstract：:Inhibition of cyclooxygenase 2 (COX-2) by the selective COX-2 inhibitor celecoxib has been suggested as potentially useful for B-cell lymphoma therapy. However, additional pharmacological activities of celecoxib have been discovered and have challenged the notion that its antitumor effects are mediated primarily via t...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2009.09.028
更新日期：2010-02-01 00:00:00
abstract：:Homoharringtonine combined aclarubicin and cytarabine (HAA) has been demonstrated to achieve a high remission rate and provide a survival advantage in acute myeloid leukemia (AML). To investigate whether HAA is an ideal induction regimen for t(8;21)AML, we retrospectively analyzed the data of 140 patients from the las...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2016.02.012
更新日期：2016-05-01 00:00:00
abstract：:Angiogenesis is a complex process controlled by the balance of a large number of regulating factors, the pro- and anti-angiogenic factors. Dysregulation of angiogenesis occurs in various pathologies and is one of the hallmarks for cancer. Recent emphasis on the microenvironment's influence in chronic lymphocytic leuke...
journal_title：Leukemia research
pub_type： 杂志文章,评审
doi：10.1016/j.leukres.2012.05.021
更新日期：2012-10-01 00:00:00
abstract：:The biological progression of chronic myeloid leukaemia is often associated with secondary cytogenetic abnormalities but the molecular mechanisms underlying this progression are poorly understood. This study explores the association of c-myc gene amplification with the progression of chronic myeloid leukaemia in fourt...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/s0145-2126(98)00097-6
更新日期：1998-10-01 00:00:00
abstract：:In this study, the expression and functional characterization of currents through the CFTR (cystic fibrosis transmembrane regulator) and ORCC (outwardly rectifying chloride channels) were determined in wild-type K562 chronic human leukemia cells (K562-WT) and in its resistant counterpart, the vincristine resistant cel...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2005.02.007
更新日期：2005-09-01 00:00:00
abstract：:The concurrent development of chronic myeloid (CML) or myelomonocytic (CMML) leukemia in patients with systemic mastocytosis (SM) is a well recognized phenomenon. Although the leukemia often resembles CML in morphological and in clinical terms, a Ph-Chromosome-positive variant has not been reported in SM so far. We he...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2005.04.004
更新日期：2005-10-01 00:00:00
