Abstract:
:Matrix metalloproteinases (MMPs) and tissue inhibitor of metalloproteinases (TIMPs) regulate the structural integrity of the extracellular matrix (ECM). Constitutive expression of human TIMP-3 in human DLD colon carcinoma cells renewed serum-responses and inhibited tumour formation in nude mice. To elucidate the mechanism of TIMP-3-mediated tumour suppression, we compared parental DLD and TIMP-3 expressing DLD cells (TIMP-3/DLD), finding them to be significantly different. TIMP-3/DLD cultures have fewer mitotic cells, are delayed in G1, and die after serum starvation. TIMP-3/DLD conditioned media activates cell death on fibroblast cells. The cell death induced by serum starvation and conditioned media was inhibited by 70%, in the presence of neutralizing tumour necrosis factor alpha (TNF-alpha) antibody. TIMP-3/DLD whole cell lysate contained p55 TNF-alpha receptor, while vector/DLD lysate had p55 TNF-alpha receptor and p46 soluble TNF-alpha inhibitor. Vector/DLD conditioned media had p46, while no soluble TNF-alpha receptor was detected in TIMP-3/DLD conditioned media. In addition, FACS analysis revealed that TIMP-3/DLD cells have more TNF-alpha surface binding sites, suggesting a direct correlation between TIMP-3 expression and surface receptors. The mechanism of tumorigenic reversion induced by TIMP-3 in DLD cells may involve protection of receptors from the proteolytic activity of MMPs. Putative TIMP-3-mediated inhibition of MMPs restores the TNF-alpha p55 signalling pathway and the carcinoma cell is killed by autocrine TNF-alpha. Thus, DLD cells have specific ECM MMPs that cleave cytokines and cytokine receptors. TIMP-3 specifically inhibits MMPs involved in receptor shedding.
journal_name
Cytokinejournal_title
Cytokineauthors
Smith MR,Kung H,Durum SK,Colburn NH,Sun Ydoi
10.1006/cyto.1997.0233subject
Has Abstractpub_date
1997-10-01 00:00:00pages
770-80issue
10eissn
1043-4666issn
1096-0023pii
S1043466697902337journal_volume
9pub_type
杂志文章相关文献
CYTOKINE文献大全abstract::Interleukin (IL-) 2 and IL-15 share the IL-2 receptor betagamma c subunits (IL-2Rbetagamma c) but have specific, unique alpha receptor subunits. We studied species specificity of human (hu), simian (si), and mouse (mu) IL-15 and found that hu and si IL-15 behaved similarly in all systems investigated. Hu and mu IL-15 ...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1006/cyto.2002.1989
更新日期:2002-11-07 00:00:00
abstract::Interleukin-8 (IL-8/CXCL8) is widely expressed in fetal tissues although inflammatory changes are not seen. Circulating IL-8 is comprised of an endothelial-derived [ala-IL-8](77) isoform and another, more potent [ser-IL-8](72) secreted by most other cells; [ala-IL-8](77) can be converted into [ser-IL-8](72) by proteol...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1016/j.cyto.2008.12.022
更新日期:2009-04-01 00:00:00
abstract::Paraquat (PQ) is a herbicide which is highly pneumotoxic by generating reactive oxygen intermediates (ROI). Pro-inflammatory cytokines, particularly IL-1 and TNF, have been implicated in some ROI-mediated pathologies, including bleomycin toxicity and ischaemia/reperfusion injury. We have studied the effect of PQ on th...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1016/1043-4666(93)90045-7
更新日期:1993-09-01 00:00:00
abstract::Recently, differences in the levels of various chemokines and cytokines were reported in patients with myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS) as compared with controls. Moreover, the analyte profile differed between chronic ME/CFS patients of long duration versus patients with disease of less than...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1016/j.cyto.2015.11.018
更新日期:2016-02-01 00:00:00
abstract::Galectin-3 is a β-galactoside-binding lectin that plays an important role in the modulation of immune responses. It has been shown to aggravate joint inflammation and destruction in experimental arthritis. We investigated the role of galectin-3 in TLR-induced cell activation in human synovial fibroblasts (SF) in order...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1016/j.cyto.2015.01.016
更新日期:2015-05-01 00:00:00
abstract::Bullous pemphigoid (BP) is an autoimmune blistering skin disease characterized by the presence of autoantibodies to normal components of the hemidesmosome (BPAg1 and BPAg2). Histology of skin lesions demonstrate a subepidermal vesicle and a predominantly eosinophilic dermal cellular infiltrate. Interleukin 5 (IL-5) pl...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1006/cyto.2000.0791
更新日期:2001-01-07 00:00:00
abstract:OBJECTIVE:This study evaluates the potential of gingival crevicular fluid and serum cytokines as HIV stage biomarkers. METHODS:Gingival crevicular fluid (GCF) and serum samples from 78 HIV-positive adult male subjects (cases) and 39 HIV-negative male subjects (controls) from Mexico were examined for 17 cytokines using...
journal_title:Cytokine
pub_type: 临床试验,杂志文章,多中心研究
doi:10.1016/j.cyto.2016.12.019
更新日期:2017-03-01 00:00:00
abstract::The aim of this study was to identify key cytokine polymorphisms associated with disease susceptibility, clinical phenotype, and outcome in patients with chronic anterior uveitis (CAU) as compared to those with recurrent self-limiting anterior uveitis (RAU). One hundred fifty seven British Caucasian patients with ante...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1016/j.cyto.2006.08.003
更新日期:2006-08-01 00:00:00
abstract::Specific high titre polyclonal antibodies rapidly obtained by intralymphnode immunization of rabbits with recombinant M-CSF and LIF (< 60 micrograms/animal) have been used to develop specific, accurate and sensitive EIAs. The same batch of purified anti-M-CSF or anti-LIF Igs has been used for the coating of 96-well pl...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1006/cyto.1996.0078
更新日期:1996-07-01 00:00:00
abstract:BACKGROUND:Hypertension is one of the most prominent risk factors for coronary artery disease (CAD). Treatment of hypertension is therefore important for reducing cardiovascular events and the progression of atherosclerosis. Several treatment strategies are common in clinical practice for example the use of ACE-blocker...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1016/j.cyto.2013.02.001
更新日期:2013-05-01 00:00:00
abstract::The in vivo influence of recombinant human erythropoietin (rhEpo) and iron on human neutrophil (PMN) antimicrobial function was assessed. A total of 21 preterm infants were randomized to receive either 200 U/kg/other day of rHuEPO+12 mg/kg/day of iron (EPO+high Fe, seven infants) or 200 U/kg/other day of rhEPO+4 mg/kg...
journal_title:Cytokine
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1006/cyto.1998.0393
更新日期:1999-01-01 00:00:00
abstract::We have recently reported the identification of four novel members of the interleukin-1 (IL-1) family which we designated as IL-1 homologue 1-4 (IL-1H1-4). These proteins exhibit significant sequence homology to other members of the IL-1 family. Of these homologues, only IL-1H4 (renamed IL-1F7b) was predicted to conta...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1006/cyto.2002.0873
更新日期:2002-04-21 00:00:00
abstract:BACKGROUND:Among all cytokines involved in the pathogenesis and in the progression of psoriasis, Tumor Necrosis Factor (TNF)-alpha and interleukin (IL)-17 play a pivotal role. OBJECTIVE:The aim of the present study was to mimic a psoriatic microenvironment and to investigate the early effects of TNF-alpha and IL-17 in...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1016/j.cyto.2014.03.003
更新日期:2014-07-01 00:00:00
abstract::The hypothalamic-pituitary-adrenal (HPA) axis is stimulated during immune and inflammatory processes. Interleukin 1beta (IL-1beta) and lipopolysaccharide (LPS) are known to be potent stimulators of this axis. During postnatal development, the rat seems to be hyporesponsive to many stimuli. The effects of repeated syst...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1006/cyto.1999.0423
更新日期:1999-03-01 00:00:00
abstract::Cytotoxic action of leukocytes may be involved in the pathogenesis of inflammatory heart muscle disorders. We investigated the expression of rat leukocyte chemotactic cytokines--cytokine induced neutrophil chemoattractant (CINC) and JE--in cultured neonatal rat cardiac myocytes; CINC belongs to the interleukin 8 (IL-8...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1006/cyto.1995.0037
更新日期:1995-04-01 00:00:00
abstract::Immunoincompetence is a profound problem in end-stage renal failure patients undergoing hemodialysis, and chronic inflammation with altered serum levels of inflammation markers has been reported. Gene expression patterns have had little relevance for leukocyte research so far because of limitations in transcript level...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1016/j.cyto.2004.12.011
更新日期:2005-05-07 00:00:00
abstract::In this study we have examined the effect of agents known to perturb certain signal transduction pathways on the biological responses of target cells to stimulation with interleukin-1 (IL-1). In the murine thymoma cell line EL4, IL-1 stimulation results in the secretion of interleukin-2 (IL-2), which was subsequently ...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1016/1043-4666(91)90009-3
更新日期:1991-01-01 00:00:00
abstract:BACKGROUND:Associations between polymorphisms in interleukins and breast cancer (BC) were already investigated by many studies, yet with controversial findings. The aim of this meta-analysis was to better clarify associations between polymorphisms in interleukins and BC by combing the results of all relevant articles. ...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1016/j.cyto.2020.154988
更新日期:2020-03-09 00:00:00
abstract::Thrombopoietin (TPO) plays a pivotal role in megakaryopoiesis. TPO initiates its biological effects by binding to its receptor Mpl. A recombinant protein consisting of a carrier Fc domain linked to multiple Mpl-binding domains was constructed, and is called AMG531. To define the biological activity of AMG531, we exami...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1016/j.cyto.2003.05.001
更新日期:2004-01-21 00:00:00
abstract::Cytokine profiles were studied during 19 episodes of chemotherapy-induced neutropenia in 17 patients with haematological malignancies. Circulating concentrations of interleukin 1 alpha (IL-1 alpha), tumour necrosis factor alpha (TNF-alpha) and IL-1 receptor antagonist (IL-1ra) were measured before chemotherapy and the...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1006/cyto.1997.0223
更新日期:1997-09-01 00:00:00
abstract::We evaluated the spontaneous IL17, IFNgamma and IL10 production by peripheral blood mononuclear cells from patients affected by clinically isolated syndromes (CIS) suggestive of multiple sclerosis (MS) both in acute phase and in remission, relapsing remitting MS (RRMS) both in relapse and in remission, not-relapsing s...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1016/j.cyto.2008.08.007
更新日期:2008-10-01 00:00:00
abstract::Human interferon regulatory factor 7 (IRF7) plays an important role in the innate antiviral immune response. To date, the characteristics and functions of waterfowl IRF7 have not been clarified. This study reports the cDNA sequence, tissue distribution, and antiviral function of duck IRF7. The duck IRF7 gene has a 153...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1016/j.cyto.2018.06.001
更新日期:2019-01-01 00:00:00
abstract::Inflammation may play a major role in the pathogenesis of preeclampsia (PE). In this meta-analysis, we determined whether maternal polymorphisms and serum concentrations of tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), and interleukin-10 (IL-10) were associated with PE. All studies investigating the assoc...
journal_title:Cytokine
pub_type: 杂志文章,meta分析
doi:10.1016/j.cyto.2011.09.021
更新日期:2011-12-01 00:00:00
abstract::Pregnancy in sickle cell disease is a problem due to the adverse outcomes related to the disease. Research into the role of chemokines in sickle cell disease is available, but studies investigating the disease in pregnancy are scarce. Our data show the chemokine profiles of pregnant women with sickle cell disease comp...
journal_title:Cytokine
pub_type: 临床试验,杂志文章
doi:10.1016/j.cyto.2018.07.002
更新日期:2019-01-01 00:00:00
abstract::The development of acidic environments is a hallmark of inflammatory processes of different etiology. We have previously shown that transient exposure to acidic conditions, similar to those encountered in vivo, induces the activation of neutrophils and the phenotypic maturation of dendritic cells. We here report that ...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1016/j.cyto.2011.11.013
更新日期:2012-02-01 00:00:00
abstract:PURPOSE:Mycoplasma pneumoniae (M. pneumoniae) is a common causative agent of pneumonia in children. The aim of this study is to determine whether there is any difference in selected cytokine or chemokines response in asthmatic children compared to non-asthmatic children during acute M. pneumoniae pneumonia. METHODS:Se...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1016/j.cyto.2011.02.008
更新日期:2011-05-01 00:00:00
abstract::The induction of cytokine secretion by human peripheral blood (PB) T cells was examined. Highly purified T cells stimulated with interleukin 7 (IL-7), in the absence of co-mitogen, secreted IL-2, IL-4, IL-6 and interferon gamma (IFN-gamma) upon restimulation with phorbol ester and ionomycin. In contrast, induction of ...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1016/1043-4666(92)90006-d
更新日期:1992-11-01 00:00:00
abstract::We investigated the effects of two low doses of interferon-alpha (IFN-alpha) on nocturnal sleep in 18 healthy men by means of polysomnographic sleep recordings. At 1900h, human recombinant IFN-alpha (1000 or 10000 U/kg body weight) or placebo was administered subcutaneously. Between 2300h and 0700h subjects were allow...
journal_title:Cytokine
pub_type: 临床试验,杂志文章
doi:10.1006/cyto.1999.0587
更新日期:2000-05-01 00:00:00
abstract:OBJECTIVE:Atherosclerosis is a chronic inflammatory disease regulated by T lymphocyte subsets. Recently, CD4(+) CD25(+) Foxp3(+) regulatory T (Treg) cells and Th17 cells have been described as two distinct subsets and have the opposite effects on autoimmunity. Clinical observation has revealed that the Th17/Treg imbala...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1016/j.cyto.2009.09.007
更新日期:2010-02-01 00:00:00
abstract::It is known that both interleukin-4 (IL-4) and IL-13 are produced by Th2-type cells and share similar biological functions with each other. However, recently accumulated evidences have revealed that IL-4 may be involved in the Th1-type response. Both thymus and activation-regulated chemokine (TARC/CCL17), a ligand for...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1006/cyto.2002.1965
更新日期:2002-10-07 00:00:00